<p>Spectrophotometric method of arsenic estimation in nails has four steps: a) washing of nails, b) digestion of nails, c) arsenic generation, and finally d) reading absorbance using spectrophotometer. Although the method is a cheapest one, widely used and effective, it is time consuming, laborious and need caution while using four acids. </p><p><strong>Introduction</strong></p><p>Estimation of total arsenic in water and biological samples are important for the diagnosis of arsenicosis (Misbahuddin, 2015). Biological samples include blood, urine, hair and nail. The normal amount of arsenic in blood is very low and requires sophisticated instrument to detect. Presence of arsenic in urine indicates recent exposure of arsenic. Absence of arsenic in urine sometimes does not exclude the diagnosis of arsenicosis. High concentration of arsenic in hair and nail indicates chronic exposure. The accumulation of arsenic in hair is less than the nail. There is superstition in some countries not to give hair sample. Compared to urine or blood samples, nails have the advantage of facilitating specimen collection, storage, and transportation. Therefore, the estimation of arsenic in nail is the preferred biological sample for easy collection (Karagas et al., 1996).</p><p>Estimation of arsenic in nail can be done using spectrophotometer, atomic absorption spectrometer with hydride generator (AAS-HG), High performance liquid chromatography with hydride generation with atomic fluorescence spectrometer (HPLC-HG-AFS), inductively coupled plasma-mass spectrometry (ICP-MS) (Chen et al., 1999), proton particle-induced X-ray emission and neutron activation analysis. Among these methods spectrophotometric method using silver diethyldithiocarbamate (SDDC) is still an effective, widely used and cheapest method.</p><p>In this method, arsenic reacts with zinc in presence of acid to produce arsine gas (AsH<sub>3</sub>) which is scrubbed through lead acetate impregnated glass wool (remove hydrogen sulfide) and is absorbed in SDDC dissolved in hexamethylenetetramine. The red color developed due to arsine silver diethyldithio-carbamate reaction is measured by spectrophotometer at wavelength of 535 nm.</p><p> </p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/V9bapA0cp1g">Nail wash</a>: 3.2 min</p><p><a href="https://www.youtube.com/v/BrG2TIWOATI">Digestion of nail</a>: 7.0 min</p><p><a href="https://www.youtube.com/v/LfnNzHnIGT8">Arsine generation</a>: 10.0 min</p><p><a href="https://www.youtube.com/v/kyCP0rumD1k">Reading absorbance</a>: 1.2 min</p><p><strong> </strong></p><p> </p>