Biomethanation of Carbon Monoxide by Hyperthermophilic Artificial Archaeal Co-Cultures

Climate neutral and sustainable energy sources will play a key role in future energy production. Biomethanation by gas to gas conversion of flue gases is one option with regard to renewable energy production. Here, we performed the conversion of synthetic carbon monoxide (CO)-containing flue gases to methane (CH4) by artificial hyperthermophilic archaeal co-cultures, consisting of Thermococcus onnurineus and Methanocaldococcus jannaschii, Methanocaldococcus vulcanius, or Methanocaldococcus villosus. Experiments using both chemically defined and complex media were performed in closed batch setups. Up to 10 mol% CH4 was produced by converting pure CO or synthetic CO-containing industrial waste gases at a high rate using a co-culture of T. onnurineus and M. villosus. These findings are a proof of principle and advance the fields of Archaea Biotechnology, artificial microbial ecosystem design and engineering, industrial waste-gas recycling, and biomethanation.

Surface Modification of Stainless-Steel Membrane using a Closed-Batch iCVD Reactor for Oil/Water Separation

Oil-spill is one of the major global issues facing society in this century. The aim of this study was to develop a steel-based membrane for selective separation of oil from oil/water mixture. For this purpose, a single-step, rapid and environmentally friendly closed-batch initiated chemical vapor deposition (iCVD) method was employed to deposit hydrophobic thin film on a stainless-steel mesh. Perfluorodecyl acrylate (PFDA) and tert-butyl peroxide (TBPO) were used as monomer and initiator, respectively. Owing to the inherent vapor-based nature of iCVD method provided excellent conformal coverage on the mesh with high durability. iCVD coated mesh showed 96% oil/water separation efficiency. Highly reproducible results were obtained when the oil/water separation experiments were repeated.

Diurnal Fe(II)/Fe(III) cycling and enhanced O2 production in a simulated Archean marine oxygen oasis

The oxygenation of early Earth’s atmosphere during the Great Oxidation Event, is generally accepted to have been caused by oceanic Cyanobacterial oxygenic photosynthesis. Recent studies suggest that Fe(II) toxicity delayed the Cyanobacterial expansion necessary for the GOE. This study investigates the effects of Fe(II) on two Cyanobacteria, Pseudanabaena sp. PCC7367 and Synechococcus sp. PCC7336, in a simulated shallow-water marine Archean environment. A similar Fe(II) toxicity response was observed as reported for closed batch cultures. This toxicity was not observed in cultures provided with continuous gaseous exchange that showed significantly shorter doubling times than the closed-culture system, even with repeated nocturnal addition of Fe(II) for 12 days. The green rust (GR) formed under high Fe(II) conditions, was not found to be directly toxic to Pseudanabaena sp. PCC7367. In summary, we present evidence of diurnal Fe cycling in a simulated shallow-water marine environment for two ancestral strains of Cyanobacteria, with increased O2 production under anoxic conditions.

Fitting of the In Vitro Gas Production Technique to the Study of High Concentrate Diets

In vitro rumen fermentation systems are often adapted to forage feeding conditions, with pH values ranging in a range close to neutrality (between 6.5 and 7.0). Several attempts using different buffers have been made to control incubation pH in order to evaluate microbial fermentation under conditions simulating high concentrate feeding, but results have not been completely successful because of rapid exhaustion of buffering capacity. Recently, a modification of bicarbonate ion concentration in the buffer of incubation solution has been proposed, which, together with using rumen inoculum from donor ruminants given high-concentrate diets, allows for mimicking such conditions in vitro. It is important to consider that the gas volume recorded is in part directly produced from microbial fermentation of substrates, but also indirectly from the buffering capacity of the medium. Thus, the contribution of each (direct and indirect) gas source to the overall production should be estimated. Another major factor affecting fermentation is the rate of passage, but closed batch systems cannot be adapted to its consideration. Therefore, a simple semicontinuous incubation system has been developed, which studies the rate and extent of fermentation by gas production at the time it allows for controlling medium pH and rate of passage by manual replacement of incubation medium by fresh saliva without including rumen inoculum. The application of this system to studies using high concentrate feeding conditions will also be reviewed here.