Evaluation of chemical and microbial quality of food in northern Iran

Background Iran is one of the developing countries and foodborne diseases commonly impose problems for public health, the health care system and the economy. Therefore, this study aimed to evaluate the chemical and microbial quality of food in northern Iran. Methods This cross-sectional study was conducted in 2019. This study was performed on food samples obtained in a straightforward way while visiting food preparation and distribution centers in Babol. Tests related to different food types were performed by laboratory experts. Data collection with a checklist: date and place of sampling, number of samples, type of food, type of test, compliance of results with standards. Data were analyzed by SPSS22 and descriptive statistics, Chi-square and t-test. Results 1043 food samples were tested from 5 groups of dairy products, protein, cereals, vegetables and other food groups. The highest number of samples in the cereal group was 767 samples (73.53%). In the cereal group, most samples were breads. The pH of 11.67% of breads and the salt in 21.49% of breads did not match the standard. The blankit (sodium hydrosulfite) on bread dough were negative. Moisture, gluten, ash and pH match with the standards in all flour samples. The results of microbial tests on sweets and ice cream showed that Escherichia coli and Staphylococcus aureus and Salmonella were negative. Enterobacter aerogenes was positive in 8.20% of sweets, mold and yeast were positive in 19.58%. The results of microbial tests on buttermilk and yogurt, grilled meat and chicken sandwiches, vegetables and salads showed that bacteria Escherichia coli and Staphylococcus aureus and all microorganisms were negative. Mold tests were positive in 11.12% of juice samples. Conclusions The results showed that the foods and drinks supplied in food and drink preparation and distribution centers in Babol in 2019 were of good chemical and microbial quality. In some food groups the results of microbial and chemical testings were negative, i.e. without contamination. Less than 20% of products in the group of cereals and protein products did not match with the standards, which is a satisfactory result compared to other studies conducted in different cities of Iran. These good results for food quality can be explained bythe constructive performance of food health experts that made good controling, monitoring, and food health and hygiene education.

No Difference in Weight Loss, Glucose, Lipids and Vitamin D of Eggs for Breakfast Compared with Cereal for Breakfast during Energy Restriction

The aim of the study was to investigate the impact of consuming 2 eggs for breakfast 5 days per week compared with eating breakfast cereal in a randomized parallel study. Two energy-restricted diets with a similar energy content were compared over a 6-month period. One hundred and ten participants—aged 56 ± 16 years, BMI 34 ± 6 kg·m2, 84 women and 26 men—commenced and 76 completed the study, 33 in the egg group and 43 in the cereal group. Weight loss in completers was 8.1 kg ± 7.0 kg (8.8 ± 6.4%) in the egg group and 7.3 kg ± 4.0 kg (7.6 ± 4.6%) in the cereal group (p < 0.001 for time) but there was no differential effect of diet (p = 0.56). Vitamin D was 55 ± 18 nmol/L at baseline rose at 3 months and fell at 6 months but remained higher than baseline (p < 0.001 for time) with no difference between the groups. Vitamin D levels were inversely correlated with BMI (r = −0.22 p = 0.025) and positively with age (r = 0.26 p = 0.009), and change in Vitamin D was positively correlated with weight change at 3 and 6 months (r = 0.46 and r = 0.41 both p < 0.001). In a post-hoc analysis of obese participants there was an effect of time (p < 0.01) and a time by diet interaction (p < 0.04), such that participants in the egg group maintained the increase in Vitamin D levels at 6-months. There was no effect on glucose and no adverse effects on total and LDL cholesterol, which did not change. In conclusion, both diets achieved clinically meaningful weight loss. There were no adverse effects on LDL-cholesterol, and there may be a beneficial effect on Vitamin D in people with obesity but this remains to be investigated in a prospective study.

Effects of dietary intervention on vitamin B12 status and cognitive level of 18-month-old toddlers in high-poverty areas: a cluster-randomized controlled trial

Background The local diet in high-poverty areas in China is mainly vegetarian, and children may be more vulnerable to vitamin B12 deficiency. Objective The aims of this study were to explore the vitamin B12 status of toddlers living in high-poverty areas of China and to observe the effects of different complementary foods on the vitamin B12 status and cognitive level of these toddlers. Methods The study was nested within a cluster-randomized controlled trial implemented in 60 administrative villages (clusters) of Xichou County in which infants aged 6 months old were randomized to receive 50 g/d of pork (meat group), an equi-caloric fortified cereal supplement (fortified cereal group) or local cereal supplement (local cereal group) for one year. At 18 months, a subsample of the 180 toddlers (60 from each group) was randomly tested for serum vitamin B12 and total homocysteine (tHcy) levels, and their neurodevelopment was evaluated. Results The median serum concentrations of vitamin B12 and tHcy were 360.0 pg/mL and 8.2 μmol/L, respectively, in children aged 18 months. Serum vitamin B12 concentrations less than 300 pg/mL were found in 62 (34.4%) children, and concentrations less than 200 pg/mL were found in 30 (16.7%) children. The median vitamin B12 concentration was significantly different among the three groups (P < 0.001). The highest vitamin B12 level was demonstrated in the fortified cereal group (509.5 pg/mL), followed by the meat group (338.0 pg/mL) and the local cereal group (241.0 pg/mL). Vitamin B12 concentration was positively correlated with the cognitive score (P < 0.001) and the fine motor score (P = 0.023) of the Bayley Scales of Infant Development, 3rd Edition (BSID III) screening test. Compared to the local cereal group, children in the meat group had higher cognitive scores (P < 0.05). Conclusion In poor rural areas of China, vitamin B12 deficiency in toddlers was common due to low dietary vitamin B12 intake. Fortified cereal and meat could help improve the vitamin B12 status of children and might improve their cognitive levels. Trial registration The larger trial in which this study was nested was registered at clinical trials.gov as NCT00726102. It was registered on July 31, 2008.

Paradox of Limited Water Resources in Iran and Goals of Self-Sufficiency in Agricultural Sector

Lack of water resources in Iran, especially in recent years, has faced the agricultural sector as the most important consumer of water resources, with serious challenges. In Iran, the agricultural sector accounted for more than 90% of water consumption. However, the focus on domestic production and self-sufficiency policy in staples (wheat, barley, maize and rice) has been emphasized in general agriculture's policy. This study was conducted to estimate the imported virtual water from the imports of basic products in Iran using defined indicators during 1961-2013. Also this study investigated the possibility of achieving self-sufficiency due to the limited water resources in Iran. The results of this study showed with the increase in cereal imports, virtual water imports from 0.28 billion cubic meters in 1961 increased to 17.6 billion cubic meters in 2013 and on average about 60% of virtual water imports in strategic products is related to wheat imports during the past 53 years. Other products in cereal (barley, maize and rice) are also indicative of the general trend of increasing imports of virtual water in development plans. The estimated long-run elasticity of virtual water imports in the cereal group compared with the country's water resources also showed that with a one percent reduction in renewable water resources of the country, the virtual water import in the main cereal group will increase equivalent to 2.89 percent and the determination coefficient more than 90 percent also confirms this negative relationship. According to this result and the emphasis on the fact that renewable water resources per capita in the country is falling increasingly, it cannot be expected that domestic production could compensate for the imports of the cereal group and virtual water imports with current technology, without increasing the water productivity and without additional harm to water resources.

Efficacy of Milk-based Diets in Persistent Diarrhea: A Randomized, Controlled Trial

Objective. Previous studies have shown increased stool output when children with persistent diarrhea (PD) received milk as the predominant source of nutrition. Methods. We evaluated the efficacy of milk given in modest amounts as a part of a mixed diet in children with PD. One hundred sixteen children 3 to 24 months of age with diarrhea for between 14 days and 12 weeks were allocated to milk-based (n = 60) or milk-free (n = 56) cereal dietary regimens. The two diets were isocaloric (86.9 calories/100 g for ≤9 months; 95.6 cal/100 g for &gt; 9 months) consisting of puffed rice cereal, sugar, and oil differing in only their source of protein, which was either milk or egg white, respectively. An average of 30% of the calories were constituted by milk in the milk-cereal diet. Both diets were offered at the rate of 150 kcal/kg per day. Children receiving milk-cereal consumed an average of 1.9 g/kg lactose per day. Results. The baseline characteristics in the two groups were similar. Comparable amounts of diet were consumed in both groups. The milk-cereal group did not have higher median (range) stool output (g/kg/h) compared with the milk-free group during a 0- to 48-hour (milk-cereal, 1.7 [0.2 to 8.7]; milk-free, 1.5 [0.1 to 6.6]) or 0- to 120-hour (milk-cereal, 1.6 [0.4 to 7.2]; milk-free, 1.3 [0.1 to 7.6]) period. The percentage of weight gain was similar in the two groups, and there were no significant differences in the duration of diarrhea. Overall, 23 children had treatment failures, 10 (17%) in the milk-cereal and 13 (23.6%) in the milk-free groups. Conclusions. Our findings suggest that modest intakes of milk are well tolerated as a part of mixed diet during PD.

A comparison of the effects of feed supplements cobalt and selenium, and perennial and annual pastures on the production of medium Peppin Merino weaner sheep in south-western Australia

Two experiments on weaner production are reported. In experiment 1, the liveweight and wool production were measured in medium Peppin Merino sheep that grazed at 10.5 weanerstha 8 plots of a mixed Phalaris aquatica-subterranean clover pasture or 8 plots of annual pasture (Trifolium subterraneum cv. Woogenellup and volunteer annual grass species). This was repeated over 2 years using autumn-born sheep; 4 groups on each pasture type were offered no supplement, 2 groups a cereal supplement (340 goats), and 2 groups of supplement isoenergetic with the cereal group but having a high protein meal replace some of the cereal (250 g oats and 60 g protein). The feed supplement was offered over the summer (January-April). The sheep on 2 of the unsupplemented plots and 1 of the 2 plots receiving either a cereal or cereal + protein supplement were offered access to a composite mineral block formulated to meet the mineral requirements of sheep with the exception of cobalt and selenium. There were 16 sheep on each plot within each group of 16 weaners, 4 were given an intraruminal cobalt 'bullet', 4 were given 5 mg of selenium orally, 4 given cobalt plus selenium and 4 were untreated controls. Experiment 2 was in year 3 with spring-born weaners on the same plots. The mineral block treatment was discarded on the plots receiving supplement and the effect of supplementary feeding at the beginning of March was compared with feeding in early January; barley was also compared with oats and protein. The stocking rate was raised to 13.5 sheep/ha. There were no statistically significant differences in sheep liveweight due to pasture type in either of the years of experiment 1 or experiment 2. Supplementation with cereals or protein-fortified cereals resulted in a significantly ( P < 0.05) increased liveweight at the end of March (5.6 kg in year 1,2.4 kg in year 2 of experiment 1, and 2.5 kg in experiment 2), and wool production (0.49 kg clean wool in year 1 and 0.3 1 kg in year 2 in experiment 1, and 0.49 in experiment 2). There was a significant liveweight response on the perennial plots to selenium + cobalt in year 1 of experiment 1. All cobalt-treated sheep were heavier ( P < 0.001) in year 2. Neither selenium nor cobalt significantly affected liveweight in experiment 2. The proportion of Phalaris aquatica on the perennial pasture diminished from 18% to less than 9% by the end of year 2 in experiment 1. These results suggest that, if perennial pastures cannot be maintained, then their establishment in the south-west of Western Australia would not result in greater animal production than on annual pasture. Decisions on using supplements would be dependent upon feed and wool prices.

Carcass and meat quality of Friesian steers fed on either pasture or barley

Friesian steers were slaughtered as individual animals reached a fasted liveweight of c. 457 kg, after being on either a predominantly cereal diet (12 animals) or a predominantly pasture diet (13 animals) for at least 160 days. Those on the cereal diet grew 31% faster than the animals on pasture so that the latter group was an average of 69 days older. The carcasses of the cereal group were significantly fatter especially with regard to omental fat (+ 54%), intramuscular fat (+ 34% for M. semitendinosus) and dissectable fat from the 9–10–11 rib cut (+ 27%). These differences in composition may have been caused by either differences in growth rate or specific dietary effects. Measurements of tenderness all favoured the cereal group, the largest effect being on cold-shortened M. semitendinosus where the mean shear value was 50% greater for the pasture group. The greater age, lower percentage of intramuscular fat and greater susceptibility to cold-shortening may all be involved in these differences. The groups did not differ in ultimate muscle pH, muscle collagen content or adrenal cortical activity. A laboratory taste panel consisting of 15 members indicated that the flavour of topside roasts from the animals fed on the cereal was more acceptable.

A STUDY OF AN ENRICHED CEREAL IN CHILD FEEDING

The value of a special enriched breakfast cereal in feeding elementary school children was studied during the school year 1943 to 1944 and again during 1944 to 1945. The children used in the investigation were among those receiving a school lunch upon the advice of a physician or nurse in the school system of which the children were a part, because they had been found to be more than 10% underweight during the fall routine medical examination. Their home dietaries were inadequate, as shown by calculations and by medical and nutritional observations on the children themselves, and the school lunches failed to compensate for the poor home food intake. The duration of the study during each school year was seven months. None of the persons making the technical evaluations knew to which experimental group individual children belonged. Methods of the Study Experimental Subjects: During each of the two years during which the study proceeded, three groups of children constituted the experimental subjects, as stated; these averaged slightly over 11 years of age, and were matched initially as to age, sex, response to the medical observations and nutrition tests, family economic status, and type of school lunch received. The children were grouped as follows in this investigation: In addition to the school lunch, Group A received the enriched cereal; Group B received a common farina of equal caloric value; and Group C received no cereal at school. Of 55 children initially in each of the three experimental groups during the first