Diffuse Alopecia and Thyroid Atrophy in Sheep

Thyroid dysfunction substantially affects the quality of life due to its association with various disorders in different organs. A low intake of selenium and zinc can predispose to thyroid alterations, resulting in hypothyroidism. A deficiency of selenium and zinc causes direct and indirect skin lesions, both by the action of free radicals on the skin and by thyroid dysfunction. The aim of this study was to describe natural cases of diffuse alopecia and thyroid abnormalities in sheep with selenium and zinc deficiency. Five adult sheep presented marked and diffuse alopecia, and the residual hairs were dry and brittle. The skin was thick and crusty, with marked peeling. The triiodothyronine (T3) and thyroxine (T4) serum concentrations were below reference values for the species. Zinc and Se concentrations were low in both the serum and liver. During necropsy, cachexia associated with serous fat atrophy was observed, and the thyroid glands showed marked atrophy. Microscopically, the thyroid presented multifocal to coalescent atrophy, with atrophied and dilated follicles, macrophage infiltration, and the presence of fibrous connective tissue. The skin revealed hyperkeratosis and edema. It is concluded that thyroid atrophy, alopecia, and hyperkeratosis are associated with low serum and liver concentrations of zinc and selenium in sheep.

Vernet’s syndrome (jugular foramen syndrome) secondary to osteoma of the tympanic bulla in a young male dog

A one-year-old male neuter dog initially presented with clinical signs of pharyngitis and suspected oesophageal reflux. The dog subsequently developed unilateral (left side) Horner’s syndrome. CT examination of the head and neck revealed a large mixed bone and soft tissue density lesion filling the majority of the left tympanic bulla and extending into the jugular foramen. Marked atrophy of the left sternocephalicus and brachiocephalicus muscles was present. Following recovery from anaesthesia, the dog suffered regurgitation and aspiration, resulting in respiratory and cardiac arrest. Given the imaging findings and grave prognosis, the dog’s owners elected euthanasia. Autopsy examination revealed a tympanic bulla osteoma. The combination of cranial nerve IX, X and XI dysfunction, associated with disease of the jugular foramen, supports a diagnosis of Vernet’s syndrome in this patient.

3-Amino-1,2,4-Triazole Induces Quick and Strong Fat Loss in Mice with High Fat-Induced Metabolic Syndrome

Background. Obesity is a growing epidemic with limited effective treatments and an important risk factor for several diseases such as metabolic syndrome (MetS). In this study, we aimed to investigate the effect of 3-amino-1,2,4-triazole (ATZ), an inhibitor of catalase and heme synthesis, in a murine model for MetS. Methods. Male C57BL/6 mice with high-fat diet-induced MetS received ATZ (500 mg·kg-1·24 h-1) for 12 weeks. Results. The HFD group showed increased blood pressure and body weight, enhanced fat deposition accompanied by an increase in adipocyte diameter, and decreased lipolysis in white adipose tissue (WAT). The expression of genes related to inflammation was increased in WAT of the HFD group. Concurrently, these mice exhibited an increase in leptin, nonesterified fatty acid (NEFA), insulin, and glucose in plasma, coupled with glucose intolerance and insulin resistance. Strikingly, ATZ prevented the increase in blood pressure and the HFD-induced obesity as observed by lower body weight, WAT index, triglycerides, NEFA, and leptin in plasma. ATZ treatment also prevented the HFD-induced increase in adipocyte diameter and even induced marked atrophy and the accumulation of macrophages in this tissue. ATZ treatment also improved glucose metabolism by increasing glucose tolerance and insulin sensitivity, GLUT4 mRNA expression in WAT in parallel to decreased insulin levels. Conclusions. In the context of HFD-induced obesity and metabolic syndrome, the fat loss induced by ATZ is probably due to heme synthesis inhibition, which blocks adipogenesis by probably decreased RevErbα activity, leading to apoptosis of adipocytes and the recruitment of macrophages. As a consequence of fat loss, ATZ elicits a beneficial systemic antiobesity effect and improves the metabolic status.

Progressive Supranuclear Palsy with Wall-Eyed Bilateral Internuclear Ophthalmoplegia Syndrome: Authors’ Second Case

Wall-eyed bilateral internuclear ophthalmoplegia (WEBINO) syndrome has previously been reported in only 2 patients with progressive supranuclear palsy (PSP). Herein, we report a third case of WEBINO syndrome with PSP. The patient was an 81-year-old man who had experienced gradually increasing gait disturbance and occasional falls since the age of 78 years. At 80 years of age, he presented with cognitive impairment, parkinsonism, and oculomotor abnormalities. The oculomotor abnormalities consisted of vertical gaze palsy and loss of eye convergence. Brain magnetic resonance imaging demonstrated marked atrophy of the midbrain. He was diagnosed with PSP. At the age of 81 years, he presented with alternating extropia in his forward gaze and adduction paresis and outward nystagmus of the abducted eye in his horizontal gaze, both of which were compatible with WEBINO syndrome. Previously, we reported the first case of PSP with WEBINO syndrome, and another group recently reported a second case. In light of the previous cases and the present case, WEBINO syndrome in PSP should not be considered extremely rare. Furthermore, WEBINO syndrome has not been reported in other neurodegenerative disorders, which suggests that it might be a useful and specific diagnostic finding in PSP.

080 Electrodiagnostic and ultrasound correlates of clinical severity in ulnar neuropathy

IntroductionThis is a retrospective review of 135 consecutive patients (M:F=71:64, mean age 52.6 years; 141 arms) referred to our institution with ulnar neuropathy over a two year period. We analysed electrodiagnostic and ultrasound findings in relation to clinical severity.MethodsAll patients underwent electrodiagnostic (AAN) and ultrasound examination of the symptomatic ulnar nerve(s). Clinical severity was graded on a 4 point scale from ‘very mild’ (symptoms only) to ‘severe’ (sensory loss plus marked atrophy of ulnar-innervated hand muscles).ResultsIn ‘very mild’ neuropathies, the number of abnormal electrodiagnostic and ultrasound studies was 2 and 11 respectively, out of 54; in ‘mild’ neuropathies 19 and 25 out of 40; in ‘moderate’, 23 and 24 out of 24; and in ‘severe’, 23 and 23 out of 23. In 25 arms, (18 of which were clinically ‘severe’ or ‘moderate’), electrophysiology was abnormal but non-localising. Ultrasound showed abnormally large cross-sectional area at the elbow in 22 and diffuse nerve enlargement in three. Ultrasound identified nerve subluxation in 24 (17%) neuropathies, 58% of which were ‘very mild’, 25% ‘mild’ and 17% ‘moderate’ or ‘severe’.ConclusionIn patients with symptoms but no clinical signs, electrophysiological evidence of ulnar neuropathy was present in 3.7%, whereas abnormal nerve ultrasound, often associated with subluxation, was demonstrated in 20%. Ultrasound increased the diagnostic yield of electrophysiology in the ‘very mild’ and, to a lesser extent, the ‘mild’ neuropathies by a combined 11%, and localised the lesion in all ulnar neuropathies with abnormal but non-localising electrophysiology. Nerve subluxation was disproportionately represented amongst the clinically ‘very mild’ neuropathies with abnormal ultrasound.

Histological Effects of Smokeless Tobacco on the Endometrial Glands of the Orally Treated Female Swiss Albino Rats

Tobacco use is the leading cause of mortality all over the world. Smokeless tobacco contains higher quantities of nicotine than most cigarettes. Over the past few decades the use of tobacco among women of reproductive ages has increased. Exposure to the toxins present in the tobacco interferes with the endometrial receptivity, endometrial angiogenesis and uterine blood flow.Material and Methods: 30 adult female Swiss albino rats were randomly selected. They were divided into three groups (n=10 each). Group A was kept as control. Experimental groups B and C consisted of rats which were given 5 % and 10% of smokeless tobacco in their feed respectively. Feed and water were given ad libitum. On 31stday the animals were sacrificed. Uterus of all the animals were removed and weighed. The tissues were processed for histological examination under light microscopy using H and E and Trichrome stains.Results: A significant decrease in the weight of the uterus was observed (P value <= 0.001). The histological changes in the uterus of experimental groups revealed severe cystically dilated sub mucosal glands. Endometrial glands also showed marked atrophy (P value <= 0.001).Conclusion: From this study it can be concluded that the smokeless form of tobacco causes adverse effects on the endometrium of the female Swiss albino rats. Long term use of this form of tobacco may lead to adverse reproductive outcomes or other pathological conditions of uterus.

Case 2.27

51-year-old woman with advanced liver disease Axial fat-suppressed FSE T2-weighted images (Figure 2.27.1) and axial arterial, portal venous, and equilibrium phase postgadolinium 3D SPGR images (Figure 2.27.2) demonstrate macronodularity of the hepatic parenchyma, marked atrophy of the right hepatic lobe, and hypertrophy of the caudate lobe....

Pengaruh bone graft dan splitting osteotomy terhadap perkembangan akar gigi molar ketiga immature yang ditransplantasi The effect of bone graft and splitting osteotomy on development of the root of transplanted immature third molar

One of the basic requirements for succesfull transplantation is an adequate size of recipient site with sufficientalveolar support. If the transplantation is performed into a fresh extraction site, the recipient site can easily beadjusted to the transplantation. However, the situation is different in patient with premolar aplasia or early molarloss with marked atrophy of the alveolar process, need additional surgical procedures like bone autograft orsplitting osteotomy of the alveolar process. This paper discussed the effect of bone autograft and splitting osteotomyon root development of transplanted immature third molars. The conclusion is these additional surgical prosedureshad a negative effect on the pulpal and periodontal condition of transplantated immature third molars. This may berelated to revascularization disturbances of pulp due to nutrient insufficiency of the recipient site.

Cadmium induced histopathological changes in the stomach and small intestine of Swiss albino mice Mus musculus

The results on the effect of oral administration of cadmium chloride on the histomorphology of the stomach and small intestine of Swiss albino mice revealed severe histopathological effects in the tissues of both stomach and small intestine. There is marked atrophy of musculature, and disintegration of sub-mucosal and mucosal tissues, distortion of villi with disintegration of mucosal epithelial cells characterized by cytoplasmic vacuolization, nuclear pycnosis and nuclear fragmentation. A significant damage in the Brunner’s gland and crypts of Liberkuhn has been observed.