This chapter describes the methodology and results of the environmental archaeology research programme at Nicopolis ad Istrum in the Balkans. The aims of the research programme were: to determine the use of the major domestic animal and plant species and to explore what husbandry practices may have been employed; to examine the changing role of domestic versus wild resources; to reconstruct the likely natural environment close to Nicopolis, using the presence of particular species and their known habitats. This chapter presents examinations of epigraphic and documentary evidence found at the site, including coins and animal footprints. A large quantity of bio-archaeological data was collected, including bone fragments of mammals, birds, and fish; moderate quantities of terrestrial, freshwater, and marine mollusca; and large quantities of cereals, pulses, edible fruits, and nuts, as well as wild plants.