scholarly journals Biochemical study of sortase E2 fromStreptomyces mobaraensisand determination of transglutaminase cross‐linking sites

FEBS Letters ◽  
2019 ◽  
Vol 593 (15) ◽  
pp. 1944-1956 ◽  
Author(s):  
Anita Anderl ◽  
Cathrin Ferlemann ◽  
Marius Muth ◽  
Antonina Henkel‐Gupalo ◽  
Aileen Ebenig ◽  
...  
Keyword(s):  
Biochemical Study ◽  
Cross Linking

Biochemical study of attached macroalgae from the Madeira Archipelago and beach-cast macroalgae from the Canary Islands: multivariate analysis to determine bioresource potential

2020 ◽  
Vol 63 (3) ◽  
pp. 283-298
Author(s):  
Nuno Nunes ◽  
Sofia Valente ◽  
Sónia Ferraz ◽  
Maria Carmo Barreto ◽  
Miguel A.A. Pinheiro de Carvalho
Keyword(s):  
Biochemical Study ◽  
Algal Species ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
The North ◽  
Madeira Archipelago ◽  
Total Carbohydrates ◽  
Beach Cast

AbstractFifteen attached macroalgae from the Madeira Archipelago, comprising three green, three red and nine brown algal species, as well as two beach-cast macroalgal samples, collected along the north shore of Gran Canaria, were assessed for their biochemical properties. The analysis included the determination of total minerals, total carbohydrates, protein, lipids, chlorophyll a, total carotenoids, total phenolic content, fucoxanthin and phycobilins (allophycocyanin, phycocyanin and phycoerythrin). The results showed a high variability of biochemical composition, allowing for the targetting of specific bioresources for particular purposes, including functional foods. This work provides the foundation for a biorefinery strategy implementation plan, for which specific macroalgae may be targeted for valuable and beneficial compounds.

scholarly journals Determination of the Oligomeric State of SecYEG Protein Secretion Channel Complex Using in Vivo Photo- and Disulfide Cross-linking

2016 ◽  
Vol 291 (11) ◽  
pp. 5997-6010 ◽  
Author(s):  
Zeliang Zheng ◽  
Amy Blum ◽  
Tithi Banerjee ◽  
Qianyu Wang ◽  
Virginia Dantis ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Cross Linking ◽  
Oligomeric State

scholarly journals Single-sided NMR for the measurement of the degree of cross-linking and curing

2018 ◽  
Vol 7 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Norbert Halmen ◽  
Christoph Kugler ◽  
Eduard Kraus ◽  
Benjamin Baudrit ◽  
Thomas Hochrein ◽  
...  
Keyword(s):  
Relaxation Times ◽  
Curing Kinetics ◽  
Cross Linking ◽  
Scanning Calorimetry ◽  
First Results ◽  
Non Destructive ◽  
Kinetics Of ◽  
Good Agreement

Abstract. The degree of cross-linking and curing is one of the most important values concerning the quality of cross-linked polyethylene (PE-X) and the functionality of adhesives and resin-based components. Up to now, the measurement of this property has mostly been time-consuming and usually destructive. Within the shown work the feasibility of single-sided nuclear magnetic resonance (NMR) for the non-destructive determination of the degree of cross-linking and curing as process monitoring was investigated. First results indicate the possibility of distinguishing between PE-X samples with different degrees of cross-linking. The homogeneity of the samples and the curing kinetics of adhesives can also be monitored. The measurements show good agreement with reference tests (wet chemical analysis, differential scanning calorimetry, dielectric analysis). Furthermore, the influence of sample temperature on the characteristic relaxation times can be observed.

scholarly journals Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexandra Stützer ◽  
Luisa M. Welp ◽  
Monika Raabe ◽  
Timo Sachsenberg ◽  
Christin Kappert ◽  
...  
Keyword(s):  
Uv Light ◽  
Amino Acid Level ◽  
Dna Interaction ◽  
Cross Linking ◽  
Dna Interactions ◽  
Single Experiment ◽  
Protein Dna Interactions ◽  
Nucleosome Binding ◽  
Binding Interfaces

Abstract Protein–DNA interactions are key to the functionality and stability of the genome. Identification and mapping of protein–DNA interaction interfaces and sites is crucial for understanding DNA-dependent processes. Here, we present a workflow that allows mass spectrometric (MS) identification of proteins in direct contact with DNA in reconstituted and native chromatin after cross-linking by ultraviolet (UV) light. Our approach enables the determination of contact interfaces at amino-acid level. With the example of chromatin-associated protein SCML2 we show that our technique allows differentiation of nucleosome-binding interfaces in distinct states. By UV cross-linking of isolated nuclei we determined the cross-linking sites of several factors including chromatin-modifying enzymes, demonstrating that our workflow is not restricted to reconstituted materials. As our approach can distinguish between protein–RNA and DNA interactions in one single experiment, we project that it will be possible to obtain insights into chromatin and its regulation in the future.

Influence of phthalocyanine pigments on the properties of flame-retardant elastomeric composites based on styrene-butadiene or acrylonitrile-butadiene rubbers

e-Polymers ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 287-294
Author(s):  
Szymon Żaczek ◽  
Agnieszka Pająk ◽  
Rafał Anyszka ◽  
Grażyna Janowska
Keyword(s):  
Mechanical Properties ◽  
Flame Retardants ◽  
Oxygen Index ◽  
Cross Linking ◽  
Dicumyl Peroxide ◽  
Green Color ◽  
Atmospheric Air ◽  
Styrene Butadiene ◽  
Elastomeric Composites

AbstractA study about the influence of the impartment of phthalocyanine pigments into elastomeric composites was conducted. Acrylonitrile-butadiene (NBR) and styrene-butadiene (SBR) rubbers crosslinked by either sulfur or dicumyl peroxide were filled with non-toxic flame retardants (Mg(OH)2 or Al(OH)3) and thermally stable pigments (zinc or chloroaluminum phthalocyanines). Determination of cross-linking degree, mechanical properties, aging coefficient, thermal analysis (TG, DTG, DTA) and flammability tests (oxygen index, combustion in air) were carried out. The addition of the pigments, apart from the impartment of blue or blue-green color to elastomeric composites, in numerous cases improved their mechanical properties, thermal stability, limited flammability and allowed obtaining self-extinguishing materials in atmospheric air.

Determination of the degree of cross-linking of phenol-formaldehyde fibres

1983 ◽  
Vol 14 (6) ◽  
pp. 434-435
Author(s):  
S. G. Fedorkina ◽  
T. N. Koziorova ◽  
L. P. Chikalo
Keyword(s):  
Phenol Formaldehyde ◽  
Cross Linking
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