Supramolecular Catalysis with a Cubic Coordination Cage: Contributions from Cavity and External‐Surface Binding

2021 ◽  
pp. 241-254
Author(s):  
Christopher G. P. Taylor ◽  
Michael D. Ward
Keyword(s):  
External Surface ◽  
Surface Binding ◽  
Supramolecular Catalysis

scholarly journals Residues of Heat-Labile Enterotoxin Involved in Bacterial Cell Surface Binding

2009 ◽  
Vol 191 (9) ◽  
pp. 2917-2925 ◽  
Author(s):  
Benjamin Mudrak ◽  
Daniel L. Rodriguez ◽  
Meta J. Kuehn
Keyword(s):  
External Surface ◽  
Outer Core ◽  
Wild Type ◽  
Surface Binding ◽  
Bacterial Surface ◽  
E Coli ◽  
Cell Surface Binding ◽  
Major Virulence Factor ◽  
Heat Labile ◽  
Lps Binding

ABSTRACT Enterotoxigenic Escherichia coli (ETEC) is a leading cause of traveler's diarrhea worldwide. One major virulence factor released by this pathogen is the heat-labile enterotoxin LT, which upsets the balance of electrolytes in the intestine. After export, LT binds to lipopolysaccharide (LPS) on the bacterial surface. Although the residues responsible for LT's binding to its host receptor are known, the portion of the toxin which mediates LPS binding has not been defined previously. Here, we describe mutations in LT that impair the binding of the toxin to the external surface of E. coli without altering holotoxin assembly. One mutation in particular, T47A, nearly abrogates surface binding without adversely affecting expression or secretion in ETEC. Interestingly, T47A is able to bind mutant E. coli expressing highly truncated forms of LPS, indicating that LT binding to wild-type LPS may be due primarily to association with an outer core sugar. Consequently, we have identified a region of LT distinct from the pocket involved in eukaryotic receptor binding that is responsible for binding to the surface of E. coli.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

High-resolution gold labeling

1993 ◽  
Vol 51 ◽  
pp. 330-331
Author(s):  
James F. Hainfeld ◽  
Frederic R. Furuya ◽  
Kyra Carbone ◽  
Martha Simon ◽  
Beth Lin ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Polypeptide Chain ◽  
External Surface ◽  
Gold Cluster ◽  
Macromolecular Complex ◽  
Gold Compound ◽  
Central Cavity ◽  
Chain Folding ◽  
E Coli ◽  
Chaperonin Groel

A recently developed 1.4 nm gold cluster has been found to be useful in labeling macromolecular sites to 1-3 nm resolution. The gold compound is organically derivatized to contain a monofunctional arm for covalent linking to biomolecules. This may be used to mark a specific site on a structure, or to first label a component and then reassemble a multicomponent macromolecular complex. Two examples are given here: the chaperonin groEL and ribosomes.Chaperonins are essential oligomeric complexes that mediate nascent polypeptide chain folding to produce active proteins. The E. coli chaperonin, groEL, has two stacked rings with a central hole ∽6 nm in diameter. The protein dihydrofolate reductase (DHFR) is a small protein that has been used in chain folding experiments, and serves as a model substrate for groEL. By labeling the DHFR with gold, its position with respect to the groEL complex can be followed. In particular, it was sought to determine if DHFR refolds on the external surface of the groEL complex, or whether it interacts in the central cavity.

Kerusakan Pada Material Pipa Air Bersih Akibat General Corrosion

2019 ◽  
Vol 16 (2) ◽  
Author(s):  
M. N. Setia Nusa
Keyword(s):  
Chemical Composition ◽  
Failure Analysis ◽  
External Surface ◽  
Internal Surface ◽  
General Corrosion ◽  
Water Pipe ◽  
Corrosion Attack ◽  
Corrosion Failure ◽  
Environment Factor

Water pipe of 4.5” diameter and has been operated for 8 year having failure due to corrosion attack on its external and internal surface. It is conducted failure analysis to find out the cause of corrosion by having testing examination of visual fractography, metalography,, SEM EDAX, hardness and chemical composition. Testing and examination results show that the failure / corroded pipe has a for in of general corrosion on the external surface due to environment factor or ground factor arround the pipe and on the internal surface was influenced by excessive root weld creating protrude which then causing turbolens and deposite to accelerate corrosion attack.Pipa berdiameter 4.5” yang berfungsi mengalirkan air bersih dan telah beroperasi selama 8 tahun, terjadi kerusakan berbentuk korosi pada permukaan luar pipa dan permukaan dalam. Untuk itu dilakukan analisa kerusakan untuk mengetahui penyebab terjadinya korosi dengan pengujian dan pemeriksaan secara visual,Fractography, Metallography, SEM, EDAX, Uji Kekerasan dan Uji Komposisi Kimia. Hasil pemeriksaan dan pengujian pada pipa yang rusak / korosi berbentuk jenis general korosi pada permukaan luar pipa yang diakibatkan faktor lingkungan atau tanah disekitar pipa, sedangkan pada bagian dalam pipa dipengaruhi oleh adanya lelehan pengelasan yang kurang sempurna sehingga menimbulkan benjolan yang mengakibatkan aliran air didalam pipa tidak lancar sehingga terjadi turbolensi yang menimbulkan endapan dan mengakibatkan percepatan terjadinya korosi.Keywords: Pipe, environtment, turbolens, corrosion, failure

TECHNOLOGICAL CONTROL OF THE CONTACT PRESSURE ON THE CYLINDRICAL SUBSTRATE FROM THE SIDE OF THE COATING ADDITIVELY FORMED ON ITS EXTERNAL SURFACE

2020 ◽  
Author(s):  
Dmitriy Parshin
Keyword(s):  
Stress State ◽  
Contact Pressure ◽  
Mechanical Model ◽  
External Surface ◽  
Layer By Layer ◽  
Layer Formation ◽  
Technological Control ◽  
Manufactured Products ◽  
Arbitrary Thickness

The article gives an example of controlling the stress state parameters of additively manufactured products. The study was carried out on the basis of a developed non-classical mechanical model of the process of layer-by-layer formation of a coating of arbitrary thickness on a cylindrical substrate. The model is based on modern concepts of the mechanics of continuously growing bodies and allows one to obtain fairly simple analytical dependencies. On the basis of the latter, the problem of technological control of the evolution of contact pressure at the substrate – coating interface is solved in the article. A number of practically significant conclusions have been made.

scholarly journals Experimental Determination of the Heat Transfer Coefficient of Real Cooled Geometry Using Linear Regression Method

Energies ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 180
Author(s):  
Asif Ali ◽  
Lorenzo Cocchi ◽  
Alessio Picchi ◽  
Bruno Facchini
Keyword(s):  
Heat Transfer ◽  
Heat Flux ◽  
Heat Transfer Coefficient ◽  
Linear Regression ◽  
Surface Temperature ◽  
Transfer Coefficient ◽  
External Surface ◽  
Internal Surface ◽  
Regression Method ◽  
Thermal Transient

The scope of this work was to develop a technique based on the regression method and apply it on a real cooled geometry for measuring its internal heat transfer distribution. The proposed methodology is based upon an already available literature approach. For implementation of the methodology, the geometry is initially heated to a known steady temperature, followed by thermal transient, induced by injection of ambient air to its internal cooling system. During the thermal transient, external surface temperature of the geometry is recorded with the help of infrared camera. Then, a numerical procedure based upon a series of transient finite element analyses of the geometry is applied by using the obtained experimental data. The total test duration is divided into time steps, during which the heat flux on the internal surface is iteratively updated to target the measured external surface temperature. The final procured heat flux and internal surface temperature data of each time step is used to find the convective heat transfer coefficient via linear regression. This methodology is successfully implemented on three geometries: a circular duct, a blade with U-bend internal channel, and a cooled high pressure vane of real engine, with the help of a test rig developed at the University of Florence, Italy. The results are compared with the ones retrieved with similar approach available in the open literature, and the pros and cons of both methodologies are discussed in detail for each geometry.

scholarly journals A new lineage of mazaediate fungi in the Eurotiomycetes: Cryptocaliciomycetidae subclass. nov., based on the new species Cryptocalicium blascoi and the revision of the ascoma evolution

2021 ◽  
Vol 20 (7) ◽  
pp. 889-904
Author(s):  
M. Prieto ◽  
Javier Etayo ◽  
I. Olariaga
Keyword(s):  
New Species ◽  
Common Ancestor ◽  
External Surface ◽  
Evolutionary Relationships ◽  
New Order ◽  
Ancestral State ◽  
Multigene Phylogeny ◽  
State Reconstruction ◽  
Distinct Structure ◽  
Dark Violet

AbstractThe class Eurotiomycetes (Ascomycota, Pezizomycotina) comprises important fungi used for medical, agricultural, industrial and scientific purposes. Eurotiomycetes is a morphologically and ecologically diverse monophyletic group. Within the Eurotiomycetes, different ascoma morphologies are found including cleistothecia and perithecia but also apothecia or stromatic forms. Mazaediate representatives (with a distinct structure in which loose masses of ascospores accumulate to be passively disseminated) have evolved independently several times. Here we describe a new mazaediate species belonging to the Eurotiomycetes. The multigene phylogeny produced (7 gene regions: nuLSU, nuSSU, 5.8S nuITS, mtSSU, RPB1, RPB2 and MCM7) placed the new species in a lineage sister to Eurotiomycetidae. Based on the evolutionary relationships and morphology, a new subclass, a new order, family and genus are described to place the new species: Cryptocalicium blascoi. This calicioid species occurs on the inner side of loose bark strips of Cupressaceae (Cupressus, Juniperus). Morphologically, C. blascoi is characterized by having minute apothecioid stalked ascomata producing mazaedia, clavate bitunicate asci with hemiamyloid reaction, presence of hamathecium and an apothecial external surface with dark violet granules that becomes turquoise green in KOH. The ancestral state reconstruction analyses support a common ancestor with open ascomata for all deep nodes in Eurotiomycetes and the evolution of closed ascomata (cleistothecioid in Eurotiomycetidae and perithecioid in Chaetothyriomycetidae) from apothecioid ancestors. The appropriateness of the description of a new subclass for this fungus is also discussed.

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