Effects of the morphology of sulfobetaine zwitterionic layers grafted onto a silicone surface on improving the hydrophilic stability, anti-bacterial adhesion properties, and biocompatibility

2018 ◽  
Vol 135 (44) ◽  
pp. 46860 ◽  
Author(s):  
Qin Cao ◽  
Shuqing Wu ◽  
Liying Wang ◽  
Xuetao Shi ◽  
Guangji Li
Keyword(s):  
Bacterial Adhesion ◽  
Adhesion Properties ◽  
Silicone Surface

scholarly journals Analyses of Biofilm on Implant Abutment Surfaces Coating with Diamond-Like Carbon and Biocompatibility

2017 ◽  
Vol 28 (3) ◽  
pp. 317-323 ◽  
Author(s):  
Patricia Milagros Maquera Huacho ◽  
Marianne N. Marques Nogueira ◽  
Fernanda G. Basso ◽  
Miguel Jafelicci Junior ◽  
Renata S. Francisconi ◽  
...  
Keyword(s):  
Dental Implant ◽  
Bacterial Adhesion ◽  
Quantitative Polymerase Chain Reaction ◽  
Antimicrobial Properties ◽  
Human Keratinocytes ◽  
Diamond Like Carbon ◽  
Adhesion Properties ◽  
E Coli ◽  
Dlc Coating ◽  
Coated Surfaces

Abstract The aim of this study was to evaluate the surface free energy (SFE), wetting and surface properties as well as antimicrobial, adhesion and biocompatibility properties of diamond-like carbon (DLC)-coated surfaces. In addition, the leakage of Escherichia coli through the abutment-dental implant interface was also calculated. SFE was calculated from contact angle values; R a was measured before and after DLC coating. Antimicrobial and adhesion properties against E. coli and cytotoxicity of DLC with human keratinocytes (HaCaT) were evaluated. Further, the ability of DLC-coated surfaces to prevent the migration of E. coli into the external hexagonal implant interface was also evaluated. A sterile technique was used for the semi-quantitative polymerase chain reaction (semi-quantitative PCR). The surfaces showed slight decreases in cell viability (p<0.05), while the SFE, R a, bacterial adhesion, antimicrobial, and bacterial infiltration tests showed no statistically significant differences (p>0.05). It was concluded that DLC was shown to be a biocompatible material with mild cytotoxicity that did not show changes in R a, SFE, bacterial adhesion or antimicrobial properties and did not inhibit the infiltration of E. coli into the abutment-dental implant interface.

Adhesion properties of potentially probioticLactobacillus kefirito gastrointestinal mucus

2013 ◽  
Vol 81 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Paula Carasi ◽  
Nicolás M. Ambrosis ◽  
Graciela L. De Antoni ◽  
Philippe Bressollier ◽  
María C. Urdaci ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Significant Proportion ◽  
Surface Protein ◽  
Protein S ◽  
Surface Proteins ◽  
Binding Properties ◽  
Added Sugar ◽  
Adhesion Properties ◽  
Probiotic Strains ◽  
Lactobacillus Kefiri

We investigated the mucus-binding properties of aggregating and non-aggregating potentially probiotic strains of kefir-isolatedLactobacillus kefiri, using different substrates. All the strains were able to adhere to commercial gastric mucin (MUCIN) and extracted mucus from small intestine (SIM) and colon (CM). The extraction of surface proteins from bacteria using LiCl or NaOH significantly reduced the adhesion of three selected strains (CIDCA 8348, CIDCA 83115 and JCM 5818); although a significant proportion (up to 50%) of S-layer proteins were not completely eliminated after treatments. The surface (S-layer) protein extracts from all the strains ofLb. kefiriwere capable of binding to MUCIN, SIM or CM, and no differences were observed among them. The addition of their own surface protein extract increased adhesion of CIDCA 8348 and 83115 to MUCIN and SIM, meanwhile no changes in adhesion were observed for JCM 5818. None of the seven sugars tested had the ability to inhibit the adhesion of whole bacteria to the three mucus extracts. Noteworthy, the degree of bacterial adhesion reached in the presence of their own surface protein (S-layer) extract decreased to basal levels in the presence of some sugars, suggesting an interaction between the added sugar and the surface proteins. In conclusion, the ability of these food-isolated bacteria to adhere to gastrointestinal mucus becomes an essential issue regarding the biotechnological potentiality ofLb. kefirifor the food industry.

scholarly journals Do graphene oxide nanostructured coatings mitigate bacterial adhesion?

2019 ◽  
Vol 6 (9) ◽  
pp. 2863-2875 ◽  
Author(s):  
Karl Wuolo-Journey ◽  
Sara BinAhmed ◽  
Elise Linna ◽  
Santiago Romero-Vargas Castrillón
Keyword(s):  
Graphene Oxide ◽  
Bacterial Adhesion ◽  
Polymer Brushes ◽  
Nanostructured Coatings ◽  
Hydrophilic Polymer ◽  
Adhesion Properties

Graphene oxide (GO) is a biocidal nanomaterial, but is it also anti-adhesive? Here we show that GO-based coatings exhibiting low bacterial adhesion properties can be formed by edge-tethering GO nanosheets to hydrophilic polymer brushes.

scholarly journals Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria

2007 ◽  
Vol 73 (8) ◽  
pp. 2653-2660 ◽  
Author(s):  
Rainer Müller ◽  
Gerhard Gröger ◽  
Karl-Anton Hiller ◽  
Gottfried Schmalz ◽  
Stefan Ruhl
Keyword(s):  
Surface Chemistry ◽  
Bacterial Adhesion ◽  
Bacterial Attachment ◽  
Bacterial Adherence ◽  
Model Organisms ◽  
Adhesion Properties ◽  
Attached Bacteria ◽  
Overlay Method ◽  
Biomaterial Surfaces

ABSTRACT For quantification of bacterial adherence to biomaterial surfaces or to other surfaces prone to biofouling, there is a need for methods that allow a comparative analysis of small material specimens. A new method for quantification of surface-attached biotinylated bacteria was established by in situ detection with fluorescence-labeled avidin-D. This method was evaluated utilizing a silicon wafer model system to monitor the influences of surface wettability and roughness on bacterial adhesion. Furthermore, the effects of protein preadsorption from serum, saliva, human serum albumin, and fibronectin were investigated. Streptococcus gordonii, Streptococcus mitis, and Staphylococcus aureus were chosen as model organisms because of their differing adhesion properties and their clinical relevance. To verify the results obtained by this new technique, scanning electron microscopy and agar replica plating were employed. Oxidized and poly(ethylene glycol)-modified silicon wafers were found to be more resistant to bacterial adhesion than wafers coated with hydrocarbon and fluorocarbon moieties. Roughening of the chemically modified surfaces resulted in an overall increase in bacterial attachment. Preadsorption of proteins affected bacterial adherence but did not fully abolish the influence of the original surface chemistry. However, in certain instances, mostly with saliva or serum, masking of the underlying surface chemistry became evident. The new bacterial overlay method allowed a reliable quantification of surface-attached bacteria and could hence be employed for measuring bacterial adherence on material specimens in a variety of applications.

Reduced bacterial adhesion on zirconium-based bulk metallic glasses by femtosecond laser nanostructuring

2019 ◽  
Vol 234 (4) ◽  
pp. 387-397 ◽  
Author(s):  
Cezhi Du ◽  
Chengyong Wang ◽  
Tao Zhang ◽  
Xin Yi ◽  
Jianyi Liang ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Irradiation ◽  
Metallic Glasses ◽  
Bacterial Adhesion ◽  
Bulk Metallic Glasses ◽  
Surface Structures ◽  
Femtosecond Laser Irradiation ◽  
Adhesion Properties ◽  
Nanostructured Surfaces

As high-performing materials, bulk metallic glasses have attracted widespread attention for biomedical applications. Herein, the bacterial adhesion properties of femtosecond laser-nanostructured surfaces of four types of zirconium-based bulk metallic glasses are assessed. Laser-induced periodical surface structures and nanoparticle structures were fabricated by femtosecond laser irradiation under different energy intensities (0.23 and 2.3 J/mm2). Surface topography, roughness, wettability, and surface energy were investigated after femtosecond laser irradiation and the surface bacterial adhesion properties were explored using Escherichia coli and Staphylococcus aureus as respective representatives of Gram-negative and Gram-positive bacteria. 4′,6-Diamidino-2-phenylindole fluorescence staining was used to characterize and assess the bacterial surface coverage rate. The in vitro cytotoxicity of polished and laser-nanostructured surfaces was investigated using MC3T3-E cells. The obtained results demonstrate that femtosecond laser surface nanostructuring retained the amorphous structure of zirconium-based bulk metallic glasses and led to an obvious decrease in bacterial adhesion compared with polished surfaces. The inhibition of bacterial adhesion on laser-induced periodical surface structures was greater than on nanostructured surfaces after 24 h of bacterial incubation. In addition, femtosecond laser nanostructuring did not have an apparent effect on the cytotoxicity of zirconium-based bulk metallic glasses.

Initial Bacterial Adhesion Properties of Anodically Oxidized Ti6Al4V

2019 ◽  
Author(s):  
P. W. Doll ◽  
M. Wolf ◽  
M. Guttmann ◽  
R. Thelen ◽  
R. Ahrens ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Adhesion Properties

scholarly journals Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties

2020 ◽  
Vol 9 (8) ◽  
pp. 2627 ◽  
Author(s):  
Takuya Haraguchi ◽  
Yasunori Ayukawa ◽  
Yukie Shibata ◽  
Toru Takeshita ◽  
Ikiru Atsuta ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Calcium Chloride ◽  
Hydrothermal Treatment ◽  
Bacterial Adhesion ◽  
Chloride Solution ◽  
Cell Attachment ◽  
Calcium Chloride Solution ◽  
Adhesion Properties ◽  
Dental Implant Surface

Topographical modification of the dental implant surface is one of the main topics for the improvement of the material, however, the roughened surface has some risks for peri-implantitis. A hydrothermal treatment (HT) of titanium with calcium chloride solution was reported to improve osseointegration and soft tissue sealing without changing the surface topography; however, its mechanism is unclear. We herewith investigated the interaction between extracellular matrix (ECM) protein and HT titanium. Furthermore, we also clarified the bacterial interaction. We employed two kinds of HT, HT with water (DW-HT) and HT with calcium chloride solution (Ca-HT). As a result, the adsorptions of both laminin-332 and osteopontin onto the Ca-HT surface were enhanced. In contrast, the adsorption of albumin, which was reported to have no cell adhesion capacity, was not influenced by Ca-HT. Osteoblast adhesion onto Ca-HT was also enhanced. Although Ca-HT was reported to enhance both epithelial cell attachment strength and in vivo peri-implant epithelial bonding, the number of epithelial cell attachment was not increased even after HT. Ca-HT had no impact in the adhesion of Streptococcus gordonii. These results suggest that Ca-HT enhances cell adhesion onto titanium without increasing bacterial adhesion, and the improvement of ECM protein adsorption is supposed to contribute to cell adhesion.

Effect of Oxygen Plasma Treatment for Cell Adhesion Properties on Silicone Surface

2005 ◽  
Vol 13 (1) ◽  
pp. A8-A8
Author(s):  
Ikuko Ohsugi ◽  
Tai Yamada ◽  
Yoshikazu Inoue ◽  
Kiyoyuki Mizuno ◽  
Takayuki Okumoto ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Plasma Treatment ◽  
Oxygen Plasma ◽  
Oxygen Plasma Treatment ◽  
Adhesion Properties ◽  
Silicone Surface ◽  
Effect Of Oxygen

scholarly journals Novel Coatings to Minimize Bacterial Adhesion and Promote Osteoblast Activity for Titanium Implants

2020 ◽  
Vol 11 (2) ◽  
pp. 42 ◽  
Author(s):  
Samira E. A. Camargo ◽  
Tanaya Roy ◽  
Patrick H. Carey IV ◽  
Chaker Fares ◽  
Fan Ren ◽  
...  
Keyword(s):  
Titanium Nitride ◽  
Porphyromonas Gingivalis ◽  
Bacterial Adhesion ◽  
Titanium Implants ◽  
Sem Images ◽  
Adhesion Properties ◽  
Osteoblast Activity ◽  
Osteoblast Adhesion ◽  
Sic Coatings ◽  
Reference Strains

Titanium nitride (TiN) and silicon carbide (SiC) adhesion properties to biofilm and the proliferation of human osteoblasts were studied. Quaternized titanium nitride (QTiN) was produced by converting the surface nitrogen on TiN to a positive charge through a quaternization process to further improve the antibacterial efficiency. The SiC required a nitridation within the plasma chamber of the surface layer before quaternization could be carried out to produce quaternized SiC (QSiC). The antimicrobial activity was evaluated on the reference strains of Porphyromonas gingivalis for 4 h by fluorescence microscopy using a live/dead viability kit. All the coatings exhibited a lower biofilm coverage compared to the uncoated samples (Ti—85.2%; TiN—24.22%; QTiN—11.4%; SiC—9.1%; QSiC—9.74%). Scanning Electron Microscope (SEM) images confirmed the reduction in P. gingivalis bacteria on the SiC and TiN-coated groups. After 24 h of osteoblast cultivation on the samples, the cell adhesion was observed on all the coated and uncoated groups. Fluorescence images demonstrated that the osteoblast cells adhered and proliferated on the surfaces. TiN and SiC coatings can inhibit the attachment of Porphyromonas gingivalis and promote osteoblast adhesion on the titanium used for implants. These coatings may possess the ability to prevent the development of peri-implantitis and stimulate osteointegration.

scholarly journals Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

2021 ◽  
Author(s):  
Johannes Mischo ◽  
Thomas Faidt ◽  
Ryan B. McMillan ◽  
Johanna Dudek ◽  
Gubesh Gunaratnam ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Bacterial Adhesion ◽  
Adhesion Force ◽  
Experimental Studies ◽  
Medical Application ◽  
Good Alternative ◽  
Adhesion Properties ◽  
Natural Materials ◽  
Rupture Length ◽  
Composition Structure

AbstractResearch into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of adhesins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples and the change in the macromolecules adsorbed over time. We found that the adhesion properties of S. aureus were similar on both samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

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