Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 ( ADH2 ) promoter and development of synthetic promoters

2021 ◽  
pp. 2100332
Author(s):  
Fidan Erden‐Karaoğlan ◽  
Mert Karaoğlan ◽  
Gürkan Yılmaz ◽  
Semiramis Yılmaz ◽  
Mehmet İnan
Keyword(s):  
Pichia Pastoris ◽  
Alcohol Dehydrogenase ◽  
Deletion Analysis ◽  
Synthetic Promoters ◽  
Adh2 Promoter ◽  
Alcohol Dehydrogenase 2

scholarly journals trans activation of human alcohol dehydrogenase gene expression in hepatoma cells by C/EBP molecules bound in a novel arrangement just 5' and 3' to the TATA box.

1990 ◽  
Vol 10 (9) ◽  
pp. 5007-5010 ◽  
Author(s):  
M J Stewart ◽  
M L Shean ◽  
G Duester
Keyword(s):  
Alcohol Dehydrogenase ◽  
Hepatoma Cell ◽  
Promoter Sequence ◽  
Tata Box ◽  
Hepatoma Cell Line ◽  
Human Hepatoma Cell ◽  
Base Pairs ◽  
Enhancer Binding Protein ◽  
Dehydrogenase Gene ◽  
Adh2 Promoter

A promoter sequence between nucleotide -51 and nucleotide -10 in the human alcohol dehydrogenase gene ADH2 has been shown to bind the transcription factor CCAAT/enhancer-binding protein (C/EBP). A series of 5'-end deletions of the ADH2 promoter was cotransfected with a C/EBP expression plasmid in a human hepatoma cell line, and trans activation by C/EBP was seen when at least 171 base pairs of 5'-flanking DNA was present. Mutations in the ADH2 promoter indicate that the mechanism of C/EBP trans activation involves two binding sites, one located just upstream of the TATA box and one located in an unusual location between the TATA box and the transcription start point.

Association of D2 dopamine receptor and alcohol dehydrogenase 2 genes with Polynesian alcoholics

2000 ◽  
Vol 15 (2) ◽  
pp. 97-102 ◽  
Author(s):  
S. Amadéo ◽  
E.P. Noble ◽  
M.L. Fourcade-Amadéo ◽  
C. Tetaria ◽  
M.F. Brugiroux ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dopamine Receptor ◽  
Association Studies ◽  
Molecular Genetic ◽  
Genetic Association Studies ◽  
Allele Frequencies ◽  
Racial Groups ◽  
D2 Dopamine Receptor ◽  
Racial Origin ◽  
Alcohol Dehydrogenase 2

SummaryAlleles of the D2 dopamine receptor (DRD2) and the alcohol dehydrogenase 2 (ADH2) genes were determined in 69 French Polynesian alcoholic patients and 57 controls matched for racial origin. Three racial groups were studied: pure Polynesians (PP), Polynesians mixed with Caucasian (PCA) ancestry and Polynesians mixed with Chinese (PCH) ancestry. DRD2 A1 allele frequencies in the alcoholics compared to their controls in these groups were: PP,.26 vs .32 (P = .69); PCA, .44 vs .35 (P = .46); PCH, .40 vs 0.39 (P = .88). ADH2 1 allele frequencies in alcoholics compared to their controls groups were: PP, .56 vs .62 (P = .66); PCA, .75 vs .56 (P = .09); PCH, .78 vs .32 (P = .009). In the PCA group, the combination of the DRD2 A1 genotypes and the ADH2 1 homozygotes was strongly associated with alcoholism (P = .0027). This preliminary study shows the importance of ascertaining racial ancestry in molecular genetic association studies. Moreover, it suggests that a combination of genes are involved in susceptibility to the development of alcoholism.

scholarly journals Arsenite release on enzymic transformation of arsonomethyl substrate analogues: a potentially lethal synthesis by glycerol-3-phosphate dehydrogenase

1995 ◽  
Vol 310 (3) ◽  
pp. 983-988 ◽  
Author(s):  
E K Mutenda ◽  
M J Sparkes ◽  
H B F Dixon
Keyword(s):  
Alcohol Dehydrogenase ◽  
Rabbit Muscle ◽  
High Affinity ◽  
Good Substrate ◽  
Yeast Alcohol Dehydrogenase ◽  
Substrate Analogues ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Alcohol Dehydrogenase 2 ◽  
In Cells

The isosteric arsenical analogue of glycerol 3-phosphate, 3,4-dihydroxybutylarsonic acid, is a good substrate for rabbit muscle glycerol-3-phosphate dehydrogenase. Its oxidation is accompanied by release of arsenite. This release seems to be due to a spontaneous elimination of arsenite by 3-oxoalkylarsonic acids, as it is also observed in (1) the oxidation of 3-hydroxypropylarsonic acid by yeast alcohol dehydrogenase, (2) treatment of 3,4-dihydroxybutylarsonic acid with periodate and (3) nonenzymic transamination of the glutamate analogue 2-amino-4-arsonobutyric acid. Enzymic formation of 3-oxoalkylarsonic acids in cells can therefore be lethal, as arsenite is poisonous to most organisms because of its high affinity for dithiols such as dihydrolipoyl groups.

Association of Polymorphism in the Alcohol Dehydrogenase 2 Gene With Alcohol-Induced Testicular Atrophy

2001 ◽  
Vol 25 (s2) ◽  
pp. 16S-18S ◽  
Author(s):  
Masayoshi Yamauchi ◽  
Kunihiko Takeda ◽  
Kazuhiko Sakamoto ◽  
Yasuyuki Searashi ◽  
Shinichiro Uetake ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Testicular Atrophy ◽  
Alcohol Dehydrogenase 2
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