Bioassay Directed Fractionation of Petroleum Ether Extract of Aerial Parts of Ceriops tagal: Isolation of Lupeol as the Nematicidal Agent against Cyst Nematode Heterodera zeae

Author(s):  
Ateeq Ahmad ◽  
Shaheen Faizi ◽  
Pirzada J. A. Siddiqui ◽  
Shahina Fayyaz ◽  
Kehkashan Khan ◽  
...  
2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Danijela Vidic ◽  
Amira Čopra-Janićijević ◽  
Mladen Miloš ◽  
Milka Maksimović

In order to determine influence of extraction method on volatile oil composition of Artemisia annua L., steam distillation, hydrodistillation, organic solvent extraction, and headspace sampling have been applied. The relative abundance of volatile compounds from the odorous aerial parts of A. annua, obtained by different extraction techniques, was analyzed by GC-MS. Exactly fifty constituents were identified. The leaf and flower essential oil yield ranged from 0.9 to 2.3% (v/w). Oxygenated monoterpenes were predominant in all samples ranged from 42.6% for steam-distilled fraction of petroleum ether extract to 70.6% for headspace of plant material. Essential oils isolated by steam distillation and hydrodistillation indicate that A. annua belongs to artemisia ketone chemotype with its relative content of 30.2% and 28.3%, respectively. The principal constituent in headspace sample of plant material was also artemisia ketone (46.4%), while headspace of petroleum ether extract had camphene (25.6%) as the major compound. The results prove the combined approaches to be powerful for the analysis of complex herbal samples.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
J Slanina ◽  
L Bøezinová ◽  
H Paulová ◽  
O Humpa

2020 ◽  
Vol 13 ◽  
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Mert Ilhan ◽  
Esra Küpeli Akkol

Background: The fruits with the seeds of Dracunculus vulgaris Schott. (Araceae) are used against inflammatory diseases in Turkey. Objective: Present study was designed to justify this folkloric usage type of the plant. Therefore, the aim of this study is to investigate the anti-inflammatory activity of D. vulgaris. Methods: Petroleum ether, ethyl acetate and methanol extracts were prepared from the fruits, successively. Carrageenan-, serotonin-, and prostaglandin E2-induced hind paw edema; acetic acid–induced capillary permeability and 12-O-tetradecanoyl-phorbol-13-acetate–induced mouse ear edema models were used to assess the antiinflammatory activity of the extracts. The analgesic activity was experienced by using p-benzoquinone-induced abdominal constriction test. Results: The petroleum ether extract displayed the highest activities in all of the used test models compared with the control group. Therefore, the constituents of this extract were determined by using gas chromatography–mass spectroscopy (GC–MS). Linoleic acid was found to be major constituent of the petroleum ether extract of D. vulgaris. Conclusion: This study has provided some justification for the folkloric use of the plant.


2021 ◽  
Author(s):  
Xierenguli Halike ◽  
Jin-Yu Li ◽  
Pengfei Yuan ◽  
Kaimeiliya Yasheng ◽  
Min Chen ◽  
...  

Brassica rapa L. is one of the most popular traditional food with a variety of biological activities. In this study, the petroleum ether extract of B. rapa was separated by...


Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.


2019 ◽  
Vol 9 (2) ◽  
pp. 330-335
Author(s):  
Namrata Omhare ◽  
Pratibha Sahu ◽  
Shailee Gautam ◽  
Nidhi Jain

In present investigation was carried out to screening of hair growth promoting potentiality of petroleum ether extract of Abrus precatorius leaf. Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of petroleum ether of Abrus precatorius was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The petroleum ether extract of Abrus precatorius showed a very good hair growth promoting activity at a dose of 300 mg/kg which was comparable to that of 2% minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. Keywords: Abrus precatorius Linn, Anagen, Catagen, Telogen, Minoxidil


1969 ◽  
Vol 42 (3) ◽  
pp. 185-195
Author(s):  
Conrado F. Asenjo ◽  
Luis Amorós Marín ◽  
Wanda Torres ◽  
Alice Del Campillo

1. Serial extraction of West Indian mahogany wood with selective solvents was performed. 2. The petroleum ether extract was the only fraction to exhibit promising termite-repellent activity. 3. Mahogany blocks subjected to petroleum ether extraction became susceptible to termite attack. 4. A waxy substance (P2 fraction) separated from the petroleum ether extract at times showed high termite-repellent activity. The termite-repellent principle in the P2 fraction seems to be a labile one, as the termite-repellent activity varied greatly from batch to batch. 5. The P2 fraction is a mixture of two major components and of at least a third minor one. As far as we have been able to determine this mixture, at the elemental level, contains carbon, hydrogen, and oxygen only, while esters have been the only compounds definitely characterized at the structural level.


2014 ◽  
Vol 57 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Tadeusz Krzaczek ◽  
Helena D. Smolarz

Hydrocarbons, aliphatic alcohols, triterpenes and sterols were shown to occur in the herb, <em>Tragopogon orientalis</em> L. The following were obtained in crystalline form: n-triacontan, hexacosanol, (β-amyrin and lupeol. The presence of (β-sitosterol, stigmasterol, cholesterol and four unidentified sterols was demonstrated by gas chromatography.


2018 ◽  
Vol 4 (1) ◽  
pp. 14-17
Author(s):  
Gamit Rakesh ◽  
◽  
Patel Amit G ◽  
VJ Shukla ◽  
MB Nariya ◽  
...  

‘Preliminary phytochemical analysis of successive extracts, fluorescence analysis and HPTLC of C. macleodiileaves has been planned in the current study.’ The extracts of the dried coarse powdered of C. macleodii leaves obtained using different polarities solventsin succession were tested for the presence of various active phytoconstituents. Fluorescence analysis was investigated andunsaponifiable fraction of petroleum ether extract was also observed forchromatographic evaluations. The outcome suggest that the existence of various dynamic phytoconstituents having particular solubility in selected solvents of different polarities used in succession. The fluorescent analysis under the visible and UV light by treatment of diverse chemical reagents showed diverse colours. HPTLC profile of unsaponifiable fraction showed 10 and 3 spots at 254 nm and 366 nm radiation respectively. After spraying with diluted H2SO4, it showed 5 and 6 spots at 254 nm and 366 nm radiations respectively. The presence of active constituents in different polarities solvent, which were used in progression suggesting the significance of the solvent as a conclusive factor. Further the data suggests that, the successive extractions using Petroleum ether, chloroform, methanol and water solvents of different polarities would maximize the exploitation of the diverse bioactive compounds. Study concluded that it would be help to isolate and characterize the different pharmacologically dynamic principles of the C. macleodii such as mouth sores, treating jaundice, hepatoprotective and also as an aphrodisiac. It shows their importance for supporting their claimed uses and biological activities such as wound healing.


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