scholarly journals Phytochemical analysis of successive extracts of the Cordia macleodii leaves Hook.: A Folklore medicinal plant

2018 ◽  
Vol 4 (1) ◽  
pp. 14-17
Author(s):  
Gamit Rakesh ◽  
◽  
Patel Amit G ◽  
VJ Shukla ◽  
MB Nariya ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Uv Light ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Fluorescence Analysis ◽  
Phytochemical Analysis ◽  
Unsaponifiable Fraction ◽  
Ether Extract ◽  
Chemical Reagents ◽  
Chloroform Methanol

‘Preliminary phytochemical analysis of successive extracts, fluorescence analysis and HPTLC of C. macleodiileaves has been planned in the current study.’ The extracts of the dried coarse powdered of C. macleodii leaves obtained using different polarities solventsin succession were tested for the presence of various active phytoconstituents. Fluorescence analysis was investigated andunsaponifiable fraction of petroleum ether extract was also observed forchromatographic evaluations. The outcome suggest that the existence of various dynamic phytoconstituents having particular solubility in selected solvents of different polarities used in succession. The fluorescent analysis under the visible and UV light by treatment of diverse chemical reagents showed diverse colours. HPTLC profile of unsaponifiable fraction showed 10 and 3 spots at 254 nm and 366 nm radiation respectively. After spraying with diluted H2SO4, it showed 5 and 6 spots at 254 nm and 366 nm radiations respectively. The presence of active constituents in different polarities solvent, which were used in progression suggesting the significance of the solvent as a conclusive factor. Further the data suggests that, the successive extractions using Petroleum ether, chloroform, methanol and water solvents of different polarities would maximize the exploitation of the diverse bioactive compounds. Study concluded that it would be help to isolate and characterize the different pharmacologically dynamic principles of the C. macleodii such as mouth sores, treating jaundice, hepatoprotective and also as an aphrodisiac. It shows their importance for supporting their claimed uses and biological activities such as wound healing.

scholarly journals In vitro Phytochemical Investigation of Helianthus annuus Seeds

2016 ◽  
Vol 19 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Rubab Tarannum Islam ◽  
Mir Monir Hossain ◽  
Kishor Majumder ◽  
Amjad Hossain Tipu
Keyword(s):  
Petroleum Ether ◽  
Helianthus Annuus ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
High Sensitivity ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Ether Extract

The seeds of Helianthus annuus have been investigated for the presence of various chemical components and biological activities with special emphasis to the anti-oxidant, antimicrobial, cytotoxic and thrombolytic assay. The results of standard phytochemical analysis revealed the presence of tannins, saponins, flavonoids, carbohydrates, steroids, fixed oils and vitamins. In vitro antioxidant activity of methanolic seed extract of H. annuus was determined by DPPH free radical scavenging assay, which demonstrated very significant antioxidant potential compared to standard antioxidant (ascorbic acid). In case of antimicrobial assay, the methanolic and ethyl acetate extract of H. annuus seeds showed high sensitivity to Salmonella typhi and moderate sensitivity to Pseudomonous aeuregenosa. The petroleum ether extract revealed high sensitivity to P. aeuregenosa. The methanolic extract of H. annuus displayed LC50 1.2 ug/ml and its petroleum ether extract showed LC50 of 1.1 ug/ml, which indicated significant cytotoxicity.Bangladesh Pharmaceutical Journal 19(1): 100-105, 2016

scholarly journals The petroleum ether extraction of Brassica Rapa L. induces apoptosis of Lung adenocarcinoma cells via mitochondria-dependent pathway

2021 ◽  
Author(s):  
Xierenguli Halike ◽  
Jin-Yu Li ◽  
Pengfei Yuan ◽  
Kaimeiliya Yasheng ◽  
Min Chen ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Brassica Rapa ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Traditional Food ◽  
Ether Extract ◽  
Ether Extraction ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
Dependent Pathway

Brassica rapa L. is one of the most popular traditional food with a variety of biological activities. In this study, the petroleum ether extract of B. rapa was separated by...

scholarly journals ANTIFUNGAL ACTIVITY OF PIMENTA DIOICA (L.) MERRIL AN AROMATIC MEDICINAL TREE

2016 ◽  
Vol 8 (10) ◽  
pp. 92 ◽  
Author(s):  
Akhilesh Bhat ◽  
Raveesha K. A.
Keyword(s):  
Antifungal Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microsporum Canis ◽  
Ether Extract ◽  
Disc Diffusion Method ◽  
Rf Values ◽  
Pimenta Dioica

<p><strong>Objective</strong>:<strong> </strong>To assess the antifungal activities of the extracts of leaves of <em>Pimenta dioica</em> against human pathogenic fungi and identify the class of phytochemical responsible for the biological activity.</p><p><strong>Methods</strong>:<strong> </strong>The shade dried, and powdered leaves of <em>Pimenta dioica</em> were subjected to polarity based solvent extraction by soaking. The extracts were dried in a rotary flash evaporator and subjected to antifungal activity assay against <em>Candida albicans</em>, <em>Microsporum canis </em>and<em> M. gypseum</em>, by disc diffusion method. Antifungal active petroleum ether extract was subjected to phytochemical analysis to identify the active compound. The extract was then subjected to Thin Layer Chromatography (TLC) and Bioautography, by agar overlay method. The fractions were collected by Preparative TLC method and subjected to antifungal activity assay by disc diffusion method. Further, the Libermann-Burchard test was conducted to detect the broad group of compounds responsible for the bioactivity.</p><p><strong>Results</strong>:<strong> </strong>Among the five extracts viz. petroleum ether, chloroform, ethyl acetate, methanol and aqueous, the petroleum ether extract showed significant antifungal activity against <em>Microsporum canis</em> (50 mm), <em>Microsporum gypseum</em> (60 mm) and <em>Candida albicans</em> (23 mm). Terpenoids, tannins and flavonoids were found to be the chief constituents of the petroleum ether extract. The TLC of the extract revealed 7 spots with the Rf values 0.37, 0.42, 0.45, 0.52, 0.59, 0.76, 0.96 with the solvent system of hexane and acetone (1:0.5). The bioautography assay revealed inhibitory activity in the spots at Rf values 0.52 and 0.59. These fractions obtained by preparative TLC also confirmed the same. The phytochemical analysis of the fractions on the TLC plate suggests that the compound is triterpene or steroid.</p><p><strong>Conclusion</strong>:<strong> </strong>The antifungal activity of <em>Pimenta dioica</em> and the bioactive phytochemical has been reported in the present study. Preliminary phytochemical analysis has indicated that the compounds are triterpenes or steroids. The results indicate that the active compounds are potential lead molecules for new antifungal drugs in the management of infectious fungi.</p>

COMPARATIVE ASSESSMENT, PRELIMINARY SCREENING AND GC- FID PHYTOCHEMICAL ANALYSIS OF AQUEOUS AND PETROLEUM ETHER EXTRACTS OF PHYLLANTHUS AMARUS LEAVES.

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
E.P Berezi ◽  
E. Mirinn ◽  
P.K. Berezi ◽  
A. E. Soroh
Keyword(s):  
Petroleum Ether ◽  
Total Concentration ◽  
Petroleum Ether Extract ◽  
Phytochemical Analysis ◽  
Phyllanthus Amarus ◽  
Ether Extract ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
Traditional Remedies ◽  
Phytochemical Constituents

The Gas Chromatography– Flame lonization Detector (GC-FID) method was employed in this study to evaluate the phytochemical constituents of both the aqueous and petroleum ether extracts of phyllanthus amarus leaves. Preliminary phytochemical screening showed the presence of Alkaloids, Flavonoids Glycosides, Phenols, Saponins, and Tannins. Further analysis revealed a total concentration of Phenol as 82.95mg/100g (4.46%) in the aqueous extract, Tannins 722.77mg/100g(38.82%),Alkaloids was 466.82mg/100(25.07%) while in the petroleum ether extract, Flavonoids was 472.59mg/100g (29.50%), Saponins 12.60mg/100g (0.79%) and Glycosides 18.63mg/100g (1.16%). These results are suggestive that P.amarus is endowed with phytochemicals that contains therapeutic potencies which support its use as traditional remedies for the treatment of variety of ailments.

scholarly journals Some phytochemical analyses of different extracts of the cogon grass Imperata cylindrica from Mizoram, India

2018 ◽  
Vol 18 (4) ◽  
pp. 120-124
Author(s):  
P.B. Lalthanpuii ◽  
Zarzokimi ◽  
K. Lalchhandama
Keyword(s):  
Petroleum Ether ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Chloroform Extract ◽  
Imperata Cylindrica ◽  
Total Phenolic ◽  
Ether Extract ◽  
Flavonoid Content ◽  
Chloroform Methanol

Cogon grass, Imperata cylindrica (L.) Räuschel, was studied for its flavonoid and phenol contents. A series of extracts of the underground (rhizome-root) parts was prepared by hot extraction using solvents of different polarities such as chloroform, methanol, and petroleum ether. The total flavonoid content of the plant extracts was determined based on the reaction with aluminum chloride, sodium nitrite, and sodium hydroxide. Quercetin was used as a standard reference. Chloroform extract showed highest content of flavonoids with 30.88 mg QE/g dry wt., followed by petroleum ether extract which was 22.05 mg mg QE/g dry wt., and methanol extract has the least value with 7.35 mg QE/g dry wt. The total phenolic content of the plant was estimated based on the reaction of Folin-Ciocalteu reagent using gallic acid as the standard reference. Again, the chloroform extract had the highest content with 7.54 mg GAE/g dry wt., while methanol extract contained 5.03 mg GAE/g dry wt., and petroleum ether extract contained 3.63 mg mg GAE/g dry wt. Our study shows that I. cylindrica is a good source of antioxidants.

PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL SCREENING OF CALENDULA OFFICINALIS (L) AND CATHARANTHUS ROSEUS (L.) AGAINST PATHOGENIC BACTERIA

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (06) ◽  
pp. 24-31
Author(s):  
R Tripathi ◽  
◽  
S Jain ◽  
N. D., Herenz ◽  
S Sharma ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Catharanthus Roseus ◽  
Pathogenic Bacteria ◽  
Petroleum Ether Extract ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Phytochemical Analysis ◽  
Ether Extract ◽  
Bacterial Strains ◽  
Antibacterial Studies

The aim of the present study was to investigate phytochemical and antibacterial potential of Calendula officinals L. and Catharanthus roseus L. G. Don leaves. The leaves of both plants were screened for physico-chemical analysis. Phytochemical screening was carried out to find phytoconstituents and subsequent antibacterial studies of extracts were carried out against the pathogenic bacterial strains Bacillus cereus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Salmonella typhi using Kirby bauer method. The extract with maximum antibacterial activity was further processed for TLC studies. The results of the present investigation revealed superiority of C. roseus over C. officinalis in terms of ash value. Both plants showed the presence of alkaloids, saponnins, tannins, phenolic compounds, flavonoids and carbohydrates. The antibacterial studies revealed that the petroleum ether extract of C. officinalis has been active against all strains, whereas the same extract of C. roseus showed activity against S. typhi, S. aureus and B. subtilis. Chloroform extract of C. officinalis was active against only two strains i.e. E. coli and B. subtilis. Methanolic and aqueous extracts of both plants were also found to be active against all strains. Studies of TLC analysis revealed that petroleum ether extract of C. officinalis and methanolic extract of C. roseus showed distinct RF values. Further studies are required to separate characterize and screen these components for therapeutic purposes.

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY AGAINST CLINICAL PATHOGEN OF MEDICINALLY IMPORTANT ORCHID CALANTHE MASUCA (D.DON) LINDL

2021 ◽  
pp. 5-8
Author(s):  
V. Nandagopalan ◽  
D. Kavitha
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Ether Extract ◽  
Wide Range

Calanthe masuca (D.Done) Lindl is a potential medicinal plant of terrestrial orchids. Tribal peoples were used the plants for therapeutic purpose. The present study was to evaluate the phytochemical analysis and Antimicrobial Activity of Calanthe masuca in leaf extracts. Preliminary phytochemical analysis revealed that the presence of bioactive substance in leaf extract was found that Alkaloids, Flavanoids, Phenol, Tannins, Saponins, Steroids, Terpenoids, Carboxcylic acid, Glycoside and Mucilage. Antimicrobial activity by Agar well diffusion method again six pathogenic microbes such as Staphylococcus aureus, Bacillus subtilis, E.coli and Pseudomonas aeruginosa in bacteria, Aspergillus niger and Candida albicans in fungi by using different solvent such as Aqueous, Methanol and Petroleum ether. It was observered that Methanol extract showed highest activity against the pathogen such as S.aureus (13.5 mm), B.subtilis (12.4 mm), E.coli (12.5 mm) and P.aeruginosa (13.0 mm) and then followed by the Aqueous extract and low activity showed in petroleum ether extract. The methanol extract showed signicant Antifungal activity against Aspergillus niger (14.0mm) and Candida albicans (13.0 mm) with higher concentration and Petroleum ether extract showed lower activity in fungi. The capacity of the crude concentrates of Calanthe masuca to suppress the development of microorganism which means that it has a wide range of antimicrobial potential utilized for various cures.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND TO EVALUATE THE ANTHELMINTIC ACTIVITY OF HYDRO-ALCOHOLIC AND PETROLEUM ETHER EXTRACT OF NYCTANTHES ARBOUR-TRISTIS ROOT. (FAMILY-NYCTAGINACEAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 68
Author(s):  
Akm Abdullah ◽  
Sumit Das
Keyword(s):  
Petroleum Ether ◽  
Plant Material ◽  
Methanolic Extract ◽  
Petroleum Ether Extract ◽  
Anthelmintic Activity ◽  
Phytochemical Analysis ◽  
Time Of Death ◽  
Ether Extract ◽  
Phytochemical Screening ◽  
Phenol Glycosides

Objective: To estimate the anthelmintic activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared. And studies Phytochemical constitutes by the various standard method. The anthelmintic activity of the plant was performed by a different extract of plant material were tested against adult Indian earthworms pheretima posthuma as test worms, the bioassay determines the time of paralysis and time of death. Albendazole is used as a standard reference drug.Results: The present study shows the phytochemical analysis, the anthelmintic activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol,, glycosides, saponins respectively. The anthelmintic activity of the plant extract showed significant results against all earthwormsConclusion: The study has shown that petroleum ether and methanolic extract of Nyctanthes arbortristis root have significantly determined anthelmintic activity. But the methanolic extract of Nyctanthes arbortristis root shown most significant anthelmintic activity as compared to petroleum ether extract.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND TO EVALUATE THE ANTI-MICROBIAL ACTIVITY OF HYDRO-ALCOHOLIC and PETROLEUM ETHER EXTRACT OF JASMINE ROOT (NYCTANTHES ARBOUR-TRISTIS). (FAMILY-NYCTAGINACEAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 47
Author(s):  
Abdul Samim ◽  
Sumit Das
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Microbial Activity ◽  
Plant Extract ◽  
Petroleum Ether Extract ◽  
Phytochemical Analysis ◽  
Disc Diffusion ◽  
Ether Extract ◽  
Bacterial Strains ◽  
Phytochemical Constituents

Objective: To estimate the anti-microbial activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared and studied for phytochemical constituents by using various standard methods. The antimicrobial activity of plant extract was performed on two bacterial strains and one fungal strain using disc diffusion method.Results: The present study shows the phytochemical analysis, antimicrobial activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol, terpenoids, glycosides, saponins respectively. The anti-microbial activity of the plant extract showed significant results against all three of the test organisms.Conclusion: The present study concluded that the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis (night flowering jasmine) contains the highly presence of Phytochemical constituents. The hydro-alcoholic and petroleum ether extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

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