scholarly journals Effects of Different Methods of Isolation on Volatile Composition of Artemisia annua L.

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Danijela Vidic ◽  
Amira Čopra-Janićijević ◽  
Mladen Miloš ◽  
Milka Maksimović

In order to determine influence of extraction method on volatile oil composition of Artemisia annua L., steam distillation, hydrodistillation, organic solvent extraction, and headspace sampling have been applied. The relative abundance of volatile compounds from the odorous aerial parts of A. annua, obtained by different extraction techniques, was analyzed by GC-MS. Exactly fifty constituents were identified. The leaf and flower essential oil yield ranged from 0.9 to 2.3% (v/w). Oxygenated monoterpenes were predominant in all samples ranged from 42.6% for steam-distilled fraction of petroleum ether extract to 70.6% for headspace of plant material. Essential oils isolated by steam distillation and hydrodistillation indicate that A. annua belongs to artemisia ketone chemotype with its relative content of 30.2% and 28.3%, respectively. The principal constituent in headspace sample of plant material was also artemisia ketone (46.4%), while headspace of petroleum ether extract had camphene (25.6%) as the major compound. The results prove the combined approaches to be powerful for the analysis of complex herbal samples.

Author(s):  
Akm Abdullah ◽  
Sumit Das

Objective: To estimate the anthelmintic activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared. And studies Phytochemical constitutes by the various standard method. The anthelmintic activity of the plant was performed by a different extract of plant material were tested against adult Indian earthworms pheretima posthuma as test worms, the bioassay determines the time of paralysis and time of death. Albendazole is used as a standard reference drug.Results: The present study shows the phytochemical analysis, the anthelmintic activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol,, glycosides, saponins respectively. The anthelmintic activity of the plant extract showed significant results against all earthwormsConclusion: The study has shown that petroleum ether and methanolic extract of Nyctanthes arbortristis root have significantly determined anthelmintic activity. But the methanolic extract of Nyctanthes arbortristis root shown most significant anthelmintic activity as compared to petroleum ether extract.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
J Slanina ◽  
L Bøezinová ◽  
H Paulová ◽  
O Humpa

2020 ◽  
Vol 13 ◽  
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Mert Ilhan ◽  
Esra Küpeli Akkol

Background: The fruits with the seeds of Dracunculus vulgaris Schott. (Araceae) are used against inflammatory diseases in Turkey. Objective: Present study was designed to justify this folkloric usage type of the plant. Therefore, the aim of this study is to investigate the anti-inflammatory activity of D. vulgaris. Methods: Petroleum ether, ethyl acetate and methanol extracts were prepared from the fruits, successively. Carrageenan-, serotonin-, and prostaglandin E2-induced hind paw edema; acetic acid–induced capillary permeability and 12-O-tetradecanoyl-phorbol-13-acetate–induced mouse ear edema models were used to assess the antiinflammatory activity of the extracts. The analgesic activity was experienced by using p-benzoquinone-induced abdominal constriction test. Results: The petroleum ether extract displayed the highest activities in all of the used test models compared with the control group. Therefore, the constituents of this extract were determined by using gas chromatography–mass spectroscopy (GC–MS). Linoleic acid was found to be major constituent of the petroleum ether extract of D. vulgaris. Conclusion: This study has provided some justification for the folkloric use of the plant.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aldo Tava ◽  
Daniele Ramella ◽  
Maris Grecchi ◽  
Paolo Aceto ◽  
Renato Paoletti ◽  
...  

The composition of the volatile fraction of two important forage legumes from Italian sub-alpine N.E. pastureland, namely Trifolium pratense L. subsp. pratense (red clover) and T. repens subsp. repens (white clover) were investigated. The volatile oil was obtained from the fresh aerial parts by steam distillation and analyzed by GC/FID and GC/MS. The oil yield was 0.018 and 0.021% (weight/fresh weight basis) for T. pratense and T. repens, respectively. Several classes of compounds were found in both the oils, including alcohols, aldehydes, ketones, terpenes, esters, hydrocarbons, phenolics and acids. Qualitative and quantitative differences were found.


2011 ◽  
Vol 29 (No. 3) ◽  
pp. 285-290 ◽  
Author(s):  
M.B. Hassanpouraghdam ◽  
A.B. Akhgari ◽  
M.A. Aazami ◽  
J. Emarat-Pardaz

The constituents of the volatile oil of air-dried aerial parts of Mentha pulegium L. (Lamiaceae) plants wildly growing in Northwest Iran were analysed by GC/MS. 46 components were identified, comprising 96.6% of the essential oil. Monoterpenes (78.9%) were the main class of the identified components followed by a minor proportion of sesqui-<br />terpenes (11%). Oxygenated monoterpenes (75.3%) were the major subclass of volatile oil components with menthone (38.7%), menthol (11.3%), neomenthol (10.5%), and pulegone (6.8%) as major compounds. Sesquiterpene hydrocarbons (10.6%) were the highlighted subclass of 15 carbons sesquiterpenoidal compounds with (E)-caryophyllene (4.9%) and &beta;-cubebene (2.5%) as their principle representatives. Furthermore, menthyl acetate (C<sub>12</sub> acetylated monoterpene derived compound) was contained considerable amounts (5.2%) in the essential oil. In total, volatile oil composition of M. pulegium L. plants studied in the present experiment was characterised as a new menthone type with appreciable amounts of menthol and neomenthol, and it could be used as a potential source of these high value monoterpenes in pharmaceutical and food industries.


2021 ◽  
Author(s):  
Xierenguli Halike ◽  
Jin-Yu Li ◽  
Pengfei Yuan ◽  
Kaimeiliya Yasheng ◽  
Min Chen ◽  
...  

Brassica rapa L. is one of the most popular traditional food with a variety of biological activities. In this study, the petroleum ether extract of B. rapa was separated by...


Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.


2019 ◽  
Vol 9 (2) ◽  
pp. 330-335
Author(s):  
Namrata Omhare ◽  
Pratibha Sahu ◽  
Shailee Gautam ◽  
Nidhi Jain

In present investigation was carried out to screening of hair growth promoting potentiality of petroleum ether extract of Abrus precatorius leaf. Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of petroleum ether of Abrus precatorius was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The petroleum ether extract of Abrus precatorius showed a very good hair growth promoting activity at a dose of 300 mg/kg which was comparable to that of 2% minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. Keywords: Abrus precatorius Linn, Anagen, Catagen, Telogen, Minoxidil


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