scholarly journals Creating Porcine Biomedical Models Through Recombineering

2004 ◽  
Vol 5 (3) ◽  
pp. 262-267 ◽  
Author(s):  
Margarita M. Rogatcheva ◽  
Laurie A. Rund ◽  
Kelly S. Swanson ◽  
Brandy M. Marron ◽  
Jonathan E. Beever ◽  
...  
Keyword(s):  
Genetic Information ◽  
Positional Cloning ◽  
Reverse Genetics ◽  
Genetic Model ◽  
Phenotypic Diversity ◽  
Model Organism ◽  
Artificial Chromosome ◽  
Forward Genetics ◽  
Working Definition ◽  
Definition Of

Recent advances in genomics provide genetic information from humans and other mammals (mouse, rat, dog and primates) traditionally used as models as well as new candidates (pigs and cattle). In addition, linked enabling technologies, such as transgenesis and animal cloning, provide innovative ways to design and perform experiments to dissect complex biological systems. Exploitation of genomic information overcomes the traditional need to choose naturally occurring models. Thus, investigators can utilize emerging genomic knowledge and tools to create relevant animal models. This approach is referred to as reverse genetics. In contrast to ‘forward genetics’, in which gene(s) responsible for a particular phenotype are identified by positional cloning (phenotype to genotype), the ‘reverse genetics’ approach determines the function of a gene and predicts the phenotype of a cell, tissue, or organism (genotype to phenotype). The convergence of classical and reverse genetics, along with genomics, provides a working definition of a ‘genetic model’ organism (3). The recent construction of phenotypic maps defining quantitative trait loci (QTL) in various domesticated species provides insights into how allelic variations contribute to phenotypic diversity. Targeted chromosomal regions are characterized by the construction of bacterial artificial chromosome (BAC) contigs to isolate and characterize genes contributing towards phenotypic variation. Recombineering provides a powerful methodology to harvest genetic information responsible for phenotype. Linking recombineering with gene-targeted homologous recombination, coupled with nuclear transfer (NT) technology can provide ‘clones’ of genetically modified animals.

A Working Definition of Mind

PsycCRITIQUES ◽  
2013 ◽  
Vol 58 (25) ◽  
Author(s):  
Robert Farr
Keyword(s):  
Working Definition ◽  
Definition Of

Establishing a Working Definition of User Experience for eHealth Interventions: A Scoping Review of Self-Reported User Experience Measures and Delphi Consultation with eHealth Researchers and Adolescents (Preprint)

2020 ◽  
Author(s):  
Amanda S Newton ◽  
Sonja March ◽  
Nicole D Gehring ◽  
Arlen K Rowe ◽  
Ashley D Radomski
Keyword(s):  
User Experience ◽  
Scoping Review ◽  
Descriptive Analysis ◽  
Intervention Development ◽  
Health Condition ◽  
Working Definition ◽  
Perceived Impact ◽  
Behavioural Factors ◽  
Definition Of ◽  
Ehealth Intervention

BACKGROUND Across eHealth intervention studies involving children, adolescents, and their parents, researchers have measured users’ experiences to assist with intervention development, refinement, and evaluation. To date, there are no widely agreed-on definitions or measures of ‘user experience’ to support a standardized approach for evaluation and comparison within or across interventions. OBJECTIVE We conducted a scoping review with subsequent Delphi consultation to (1) identify how user experience is defined and measured in eHealth research studies, (2) characterize the measurement tools used, and (3) establish working definitions for domains of user experience that could be used in future eHealth evaluations. METHODS We systematically searched electronic databases for published and gray literature available from January 1, 2005 to April 11, 2019. Studies assessing an eHealth intervention that targeted any health condition and was designed for use by children, adolescents, and their parents were eligible for inclusion. eHealth interventions needed to be web-, computer-, or mobile-based, mediated by the internet with some degree of interactivity. Studies were also required to report the measurement of ‘user experience’ as first-person experiences, involving cognitive and behavioural factors, reported by intervention users. Two reviewers independently screened studies for relevance and appraised the quality of user experience measures using published criteria: ‘well-established’, ‘approaching well-established’, ‘promising’, or ‘not yet established’. We conducted a descriptive analysis of how user experience was defined and measured in each study. Review findings subsequently informed the survey questions used in the Delphi consultations with eHealth researchers and adolescent users for how user experience should be defined and measured. RESULTS Of the 8,634 articles screened for eligibility, 129 and one erratum were included in the review. Thirty eHealth researchers and 27 adolescents participated in the Delphi consultations. Based on the literature and consultations, we proposed working definitions for six main user experience domains: acceptability, satisfaction, credibility, usability, user-reported adherence, and perceived impact. While most studies incorporated a study-specific measure, we identified ten well-established measures to quantify five of the six domains of user experience (all except for self-reported adherence). Our adolescent and researcher participants ranked perceived impact as one of the most important domains of user experience and usability as one of the least important domains. Rankings between adolescents and researchers diverged for other domains. CONCLUSIONS Findings highlight the various ways user experience has been defined and measured across studies and what aspects are most valued by researchers and adolescent users. We propose incorporating the working definitions and available measures of user experience to support consistent evaluation and reporting of outcomes across studies. Future studies can refine the definitions and measurement of user experience, explore how user experience relates to other eHealth outcomes, and inform the design and use of human-centred eHealth interventions.

scholarly journals What is Salience?

2020 ◽  
Vol 6 (1) ◽  
pp. 713-722
Author(s):  
Vincent Boswijk ◽  
Matt Coler
Keyword(s):  
Second Language ◽  
Second Language Acquisition ◽  
Language Acquisition ◽  
Cognitive Linguistics ◽  
Working Definition ◽  
Definition Of

AbstractA commonly used concept in linguistics is salience. Oftentimes it is used without definition, and the meaning of the concept is repeatedly assumed to be self-explanatory. The definitions that are provided may vary greatly from one operationalization of salience to the next. In order to find out whether it is possible to postulate an overarching working definition of linguistic salience that subsumes usage across linguistic subdomains, we review these different operationalizations of linguistic salience. This article focuses on salience in sociolinguistics, cognitive linguistics, second-language acquisition (SLA), and semantics. In this article, we give an overview of how these fields operationalize salience. Finally, we discuss correlations and contradictions between the different operationalizations.

In Search of a Standard: References to the Rule of Law in the Case-law of the European Court of Human Rights

ICL Journal ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 43-69
Author(s):  
Eszter Polgári
Keyword(s):  
Human Rights ◽  
Rule Of Law ◽  
Case Law ◽  
Council Of Europe ◽  
The Rule Of Law ◽  
Working Definition ◽  
European Court ◽  
Structured Analysis ◽  
Definition Of ◽  
Grand Chamber

AbstractThe present article maps the explicit references to the rule of law in the jurisprudence of the ECtHR by examining the judgments of the Grand Chamber and the Plenary Court. On the basis of the structured analysis it seeks to identify the constitutive elements of the Court’s rule of law concept and contrast it with the author’s working definition and the position of other Council of Europe organs. The review of the case-law indicates that the Court primarily associates the rule of law with access to court, judicial safeguards, legality and democracy, and it follows a moderately thick definition of the concept including formal, procedural and some substantive elements. The rule of law references are predominantly ancillary arguments giving weight to other Convention-based considerations and it is not applied as a self-standing standard.

Behavioral Effects of Volatile Anesthetics in Caenorhabditis elegans

1996 ◽  
Vol 85 (4) ◽  
pp. 901-912 ◽  
Author(s):  
Michael C. Crowder ◽  
Laynie D. Shebester ◽  
Tim Schedl
Keyword(s):  
Nervous System ◽  
Caenorhabditis Elegans ◽  
Time Course ◽  
Model Organism ◽  
Volatile Anesthetic ◽  
Volatile Anesthetics ◽  
Effective Concentration ◽  
Forward Genetics ◽  
C Elegans ◽  
Median Effective Concentration

Background The nematode Caenorhabditis elegans offers many advantages as a model organism for studying volatile anesthetic actions. It has a simple, well-understood nervous system; it allows the researcher to do forward genetics; and its genome will soon be completely sequenced. C. elegans is immobilized by volatile anesthetics only at high concentrations and with an unusually slow time course. Here other behavioral dysfunctions are considered as anesthetic endpoints in C. elegans. Methods The potency of halothane for disrupting eight different behaviors was determined by logistic regression of concentration and response data. Other volatile anesthetics were also tested for some behaviors. Established protocols were used for behavioral endpoints that, except for pharyngeal pumping, were set as complete disruption of the behavior. Time courses were measured for rapid behaviors. Recovery from exposure to 1 or 4 vol% halothane was determined for mating, chemotaxis, and gross movement. All experiments were performed at 20 to 22 degrees C. Results The median effective concentration values for halothane inhibition of mating (0.30 vol%-0.21 mM), chemotaxis (0.34 vol%-0.24 mM), and coordinated movement (0.32 vol% - 0.23 mM) were similar to the human minimum alveolar concentration (MAC; 0.21 mM). In contrast, halothane produced immobility with a median effective concentration of 3.65 vol% (2.6 mM). Other behaviors had intermediate sensitivities. Halothane's effects reached steady-state in 10 min for all behaviors tested except immobility, which required 2 h. Recovery was complete after exposure to 1 vol% halothane but was significantly reduced after exposure to immobilizing concentrations. Conclusions Volatile anesthetics selectively disrupt C. elegans behavior. The potency, time course, and recovery characteristics of halothane's effects on three behaviors are similar to its anesthetic properties in vertebrates. The affected nervous system molecules may express structural motifs similar to those on vertebrate anesthetic targets.

scholarly journals Knockdown of LIM15/DMC1 in the mushroom Coprinus cinereus by double-stranded RNA-mediated gene silencing

Microbiology ◽  
2005 ◽  
Vol 151 (11) ◽  
pp. 3669-3678 ◽  
Author(s):  
Satoshi H. Namekawa ◽  
Kazuki Iwabata ◽  
Hiroko Sugawara ◽  
Fumika N. Hamada ◽  
Akiyo Koshiyama ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sexual Development ◽  
Reverse Genetics ◽  
Model Organism ◽  
Coprinus Cinereus ◽  
Gene Repression ◽  
Mushroom Species ◽  
Double Stranded Rna ◽  
Chromosome Synapsis ◽  
Expression Construct

The basidiomycete Coprinus cinereus has many advantages as a model organism for studying sexual development and meiosis, but it has been difficult to investigate using reverse-genetics methods, such as gene disruption by homologous recombination. Here, gene repression by dsRNA-mediated gene silencing was tried as an alternative method for reverse-genetics studies. It was shown that transformation of the LIM15/DMC1 dsRNA expression construct (LIM15dsRNA) resulted in genomic insertion of LIM15dsRNA and paucity of the LIM15/DMC1 transcript. First, LIM15dsRNA was transformed into the homothallic strain AmutBmut to generate a homozygote in which both nuclei had a copy of LIM15dsRNA. The LIM15/DMC1-repressed strain showed abnormal homologous chromosome synapsis during meiosis. Basidiospore production was reduced to 16 % by the induction of dsRNA. However, approximately 60 % of basidiospores were viable. Next, a heterozygote was generated in which one nucleus had a copy of LIM15dsRNA. The phenotype was similar to that of the homozygote. These results are not only the first demonstration of dsRNA-mediated gene silencing in a member of the homobasidiomycete fungi, to which 90 % of mushroom species belong, but also the first successful use of a reverse-genetics approach in C. cinereus research.

An Evaluation of ‘Process as Product’ in OTI Sri Lanka: A Typology of Community-Level Peacebuilding Activities and Programming Recommendation

2007 ◽  
Vol 3 (3) ◽  
pp. 22-34
Author(s):  
Lee R. Briggs
Keyword(s):  
Sri Lanka ◽  
International Development ◽  
Community Setting ◽  
The United States ◽  
Lessons Learned ◽  
Community Level ◽  
Project Impact ◽  
Working Definition ◽  
Programme Development ◽  
Definition Of

This paper presents a set of best practices and lessons learned from a set of 93 impact evaluations conducted on community-level, small grants activities implemented between March 2003 and September 2007 by the Sri Lanka country programme of the Office of Transition Initiatives (OTI) of the United States Agency for International Development (USAID). It analyses the change theories that guided programme development and common trends in impact which emerged, and discusses ways in which programme staff can improve project impact. It provides a working definition of ‘process’, a key element of OTI's approach and a key concept used by facilitators to understand the work they do with groups and communities. It also delineates a general typology of peacebuilding projects likely to emerge in the community setting. Finally, it formulates a postulate for predicting and observing generic programme impact based upon the relative richness of process, which is considered useful for informing further research design.

Toward a Radical IR: Transformation, Praxis, and Critique in a (Neo)Liberal World Order

2021 ◽  
Author(s):  
Dillon Stone Tatum
Keyword(s):  
Political Change ◽  
World Order ◽  
Political Movements ◽  
Fundamental Change ◽  
Global Capital ◽  
Contemporary World ◽  
Working Definition ◽  
International Theory ◽  
Fundamental Transformation ◽  
Definition Of

Abstract Can there be a “radical IR?” Scholars have given little attention to the question of the following: where is radicalism in the discipline? I argue that not only is it possible to think about radical international theory, but that it is necessary in the contemporary world. International theorists have to grapple with developments of fundamental change, including the so-described decline of the (neo)liberal international order, transformations in global capital, and an upsurge in populist political movements that advocate for fundamental political change. In approaching the question of radicalism in IR, the article develops a working definition of radicalism as an approach to politics that focuses on the International as a whole, uses theoretical tools from the humanistic sciences to engage in an active politics of fundamental transformation, and deploys methods that are historicist, genealogical, and oriented toward “getting to the root of things.” Additionally, the paper illustrates the virtues and promises of a radical IR by using the case of (neo)liberal world order arguments to show how a radical IR could change the trajectory of these engagements.

Sign in / Sign up

Export Citation Format

Share Document