Sustainable cultivation of Haematococcus pluvialis and Chromochloris zofingiensis for the production of astaxanthin and co‐products

2021 ◽  
Author(s):  
G. K. S. H. Nishshanka ◽  
V. C. Liyanaarachchi ◽  
Malith Premaratne ◽  
Thilini U. Ariyadasa ◽  
P. H. V. Nimarshana
Keyword(s):  
Haematococcus Pluvialis ◽  
Sustainable Cultivation ◽  
Chromochloris Zofingiensis

scholarly journals Time-resolved carotenoid profiling and transcriptomic analysis reveal mechanism of carotenogenesis for astaxanthin synthesis in the oleaginous green alga Chromochloris zofingiensis

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Yu Zhang ◽  
Meicheng Shi ◽  
Xuemei Mao ◽  
Yaping Kou ◽  
Jin Liu
Keyword(s):  
Haematococcus Pluvialis ◽  
Value Added ◽  
Carotenoid Content ◽  
Integrated Analysis ◽  
Nitrogen Deprivation ◽  
Time Resolved ◽  
A Value ◽  
Total Carotenoid Content ◽  
Secondary Carotenoids ◽  
Chromochloris Zofingiensis

Abstract Background Chromochloris zofingiensis is emerging as an industrially relevant alga given its robust growth for the production of lipids and astaxanthin, a value-added carotenoid with broad applications. Nevertheless, poor understanding of astaxanthin synthesis has limited engineering of this alga for rational improvements. Results To reveal the molecular mechanism underlying astaxanthin accumulation in C. zofingiensis, here we conducted an integrated analysis by combining the time-resolved transcriptomes and carotenoid profiling in response to nitrogen deprivation (ND). A global response was triggered for C. zofingiensis to cope with the ND stress. Albeit the little variation in total carotenoid content, individual carotenoids responded differentially to ND: the primary carotenoids particularly lutein and β-carotene decreased, while the secondary carotenoids increased considerably, with astaxanthin and canthaxanthin being the most increased ones. The carotenogenesis pathways were reconstructed: ND had little effect on the carbon flux to carotenoid precursors, but stimulated astaxanthin biosynthesis while repressing lutein biosynthesis, thereby diverting the carotenoid flux from primary carotenoids to secondary carotenoids particularly astaxanthin. Comparison between C. zofingiensis and Haematococcus pluvialis revealed the distinctive mechanism of astaxanthin synthesis in C. zofingiensis. Furthermore, potential bottlenecks in astaxanthin synthesis were identified and possible engineering strategies were proposed for the alga. Conclusions Collectively, these findings shed light on distinctive mechanism of carotenogenesis for astaxanthin biosynthesis in C. zofingiensis, identify key functional enzymes and regulators with engineering potential and will benefit rational manipulation of this alga for improving nutritional traits.

Sustainable Cultivation of Haematococcus pluvialis for the Production of Natural Astaxanthin

2021 ◽  
Author(s):  
Sanuji Hasara Nishshanka ◽  
Vinoj Chamilka Liyanaarachchi ◽  
Malith Premaratne ◽  
Thilini U. Ariyadasa ◽  
Viraj Nimarshana
Keyword(s):  
Haematococcus Pluvialis ◽  
Sustainable Cultivation

Stimulated Growth of Green Algae Haematococcus Pluvialis Under Different Environmental Conditions

2018 ◽  
Vol 18 (1) ◽  
pp. 53-61
Author(s):  
Arevik Minasyan
Keyword(s):  
Growth Rate ◽  
Green Algae ◽  
Haematococcus Pluvialis ◽  
Parallel Testing ◽  
Anti Cancer ◽  
Health Promoting ◽  
Sustainable Cultivation ◽  
Anti Oxidant ◽  
Culture Growth ◽  
Cancer Agent

Abstract Haematococcus pluvialis is a genus of green algae that keeps the high interest of the scientists for decades due to its capacity to synthesize astaxanthin. Among many others, astaxanthin is one of the most demanded natural anti-oxidant and the product of the future. It has the widest usage as source of nutrient rich food and health promoting compounds. Astaxanthin has got various applications in the nutraceuticals as immune system enhancer and anti-cancer agent, in the cosmetics as anti-aging component, as additive to food, as well as in the aquaculture industries. As a key requirement for the research in this field is to find out the better conditions and media to stimulate the H.pluvialis higher growth rate to reach in the field of microalgae economically sustainable cultivation and processing. The effect of WC versus BG-11 media to enhance growth rate of Haematococcus pluvialis has been examined. This study employed a parallel testing of stimulating effect of extra vitamins versus phosphorus. Combination of mentioned parameters with gentle shacking have been determined as multiple effectors that contribute to green algae cultures to grow to high density. Importantly, this study demonstrates that extra phosphorus contribute to growth rate of H.pluvilais positively, meanwhile addition of extra vitamins in combination with extra phosphorus impact both positively and negatively to promote cells division. Shacking influences the culture growth as either stimulator or repressor.

Sustainable Cultivation Concepts for Domestic Energy Production from Biomass

2001 ◽  
Vol 20 (1) ◽  
pp. 1-14 ◽  
Author(s):  
M Karpenstein-Machan
Keyword(s):  
Energy Production ◽  
Sustainable Cultivation ◽  
Domestic Energy

A sustainable cultivation of microalgae using dairy and fish wastes for enhanced biomass and bio-product production

2021 ◽  
Author(s):  
D. Vidya ◽  
K. Nayana ◽  
M. Sreelakshmi ◽  
K. V. Keerthi ◽  
K. Sneha Mohan ◽  
...  
Keyword(s):  
Sustainable Cultivation

scholarly journals NaCl Promotes the Efficient Formation of Haematococcus pluvialis Nonmotile Cells under Phosphorus Deficiency

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 337
Author(s):  
Feng Li ◽  
Ning Zhang ◽  
Yulei Zhang ◽  
Qingsheng Lian ◽  
Caiying Qin ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Phosphorus Deficiency ◽  
Cell Formation ◽  
Animal Nutrition ◽  
Cell Factory ◽  
Cell Type ◽  
Negative Effects ◽  
Related Factors ◽  
Motile Cells ◽  
Application Prospects

Natural astaxanthin helps reduce the negative effects caused by oxidative stress and other related factors, thereby minimizing oxidative damage. Therefore, it has considerable potential and broad application prospects in human health and animal nutrition. Haematococcus pluvialis is considered to be the most promising cell factory for the production of natural astaxanthin. Previous studies have confirmed that nonmotile cells of H. pluvialis are more tolerant to high intensity of light than motile cells. Cultivating nonmotile cells as the dominant cell type in the red stage can significantly increase the overall astaxanthin productivity. However, we know very little about how to induce nonmotile cell formation. In this work, we first investigated the effect of phosphorus deficiency on the formation of nonmotile cells of H. pluvialis, and then investigated the effect of NaCl on the formation of nonmotile cells under the conditions of phosphorus deficiency. The results showed that, after three days of treatment with 0.1% NaCl under phosphorus deficiency, more than 80% of motile cells had been transformed into nonmotile cells. The work provides the most efficient method for the cultivation of H. pluvialis nonmotile cells so far, and it significantly improves the production of H. pluvialis astaxanthin.

scholarly journals Growth of Haematococcus pluvialis on a Small-Scale Angled Porous Substrate Photobioreactor for Green Stage Biomass

2021 ◽  
Vol 11 (4) ◽  
pp. 1788
Author(s):  
Thanh-Tri Do ◽  
Binh-Nguyen Ong ◽  
Tuan-Loc Le ◽  
Thanh-Cong Nguyen ◽  
Bich-Huy Tran-Thi ◽  
...  
Keyword(s):  
Light Intensity ◽  
Algal Biomass ◽  
Haematococcus Pluvialis ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Small Scale ◽  
Porous Substrate ◽  
Low Light ◽  
Green Algal ◽  
Green Stage

In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m−2·s−1, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m−2, and the dry biomass productivity at a light intensity of 80 µmol photons m−2·s−1 was 6.5 g·m−2·d−1. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin.

scholarly journals Xanthophylls from the Sea: Algae as Source of Bioactive Carotenoids

Marine Drugs ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 188
Author(s):  
Antia G. Pereira ◽  
Paz Otero ◽  
Javier Echave ◽  
Anxo Carreira-Casais ◽  
Franklin Chamorro ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Biological Effects ◽  
Algal Species ◽  
Antioxidant Properties ◽  
Laminaria Japonica ◽  
Chlorella Zofingiensis ◽  
Brown Seaweeds ◽  
Extraction And Purification ◽  
Available Information ◽  
Large Groups

Algae are considered pigment-producing organisms. The function of these compounds in algae is to carry out photosynthesis. They have a great variety of pigments, which can be classified into three large groups: chlorophylls, carotenoids, and phycobilins. Within the carotenoids are xanthophylls. Xanthophylls (fucoxanthin, astaxanthin, lutein, zeaxanthin, and β-cryptoxanthin) are a type of carotenoids with anti-tumor and anti-inflammatory activities, due to their chemical structure rich in double bonds that provides them with antioxidant properties. In this context, xanthophylls can protect other molecules from oxidative stress by turning off singlet oxygen damage through various mechanisms. Based on clinical studies, this review shows the available information concerning the bioactivity and biological effects of the main xanthophylls present in algae. In addition, the algae with the highest production rate of the different compounds of interest were studied. It was observed that fucoxanthin is obtained mainly from the brown seaweeds Laminaria japonica, Undaria pinnatifida, Hizikia fusiformis, Sargassum spp., and Fucus spp. The main sources of astaxanthin are the microalgae Haematococcus pluvialis, Chlorella zofingiensis, and Chlorococcum sp. Lutein and zeaxanthin are mainly found in algal species such as Scenedesmus spp., Chlorella spp., Rhodophyta spp., or Spirulina spp. However, the extraction and purification processes of xanthophylls from algae need to be standardized to facilitate their commercialization. Finally, we assessed factors that determine the bioavailability and bioaccesibility of these molecules. We also suggested techniques that increase xanthophyll’s bioavailability.

scholarly journals Variability in Macro- and Micronutrients of 15 Commercially Available Microalgae Powders

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 310
Author(s):  
Fabian Sandgruber ◽  
Annekathrin Gielsdorf ◽  
Anja C. Baur ◽  
Benjamin Schenz ◽  
Sandra Marie Müller ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dunaliella Salina ◽  
Haematococcus Pluvialis ◽  
Saturated Fatty Acids ◽  
Dry Weight ◽  
Chlorella Pyrenoidosa ◽  
Essential Amino Acids ◽  
Arthrospira Platensis ◽  
Omega 3 ◽  
Tetraselmis Chuii

The nutrient composition of 15 commercially available microalgae powders of Arthrospira platensis, Chlorella pyrenoidosa and vulgaris, Dunaliella salina, Haematococcus pluvialis, Tetraselmis chuii, and Aphanizomenon flos-aquae was analyzed. The Dunaliella salina powders were characterized by a high content of carbohydrates, saturated fatty acids (SFAs), omega-6-polyunsaturated fatty acids (n6-PUFAs), heavy metals, and α-tocopherol, whereas the protein amounts, essential amino acids (EAAs), omega-3-PUFAs (n3-PUFAs), vitamins, and minerals were low. In the powder of Haematococcus pluvialis, ten times higher amounts of carotenoids compared to all other analyzed powders were determined, yet it was low in vitamins D and E, protein, and EAAs, and the n6/n3-PUFAs ratio was comparably high. Vitamin B12, quantified as cobalamin, was below 0.02 mg/100 g dry weight (d.w.) in all studied powders. Based on our analysis, microalgae such as Aphanizomenon and Chlorella may contribute to an adequate intake of critical nutrients such as protein with a high content of EAAs, dietary fibers, n3-PUFAs, Ca, Fe, Mg, and Zn, as well as vitamin D and E. Yet, the nutritional value of Aphanizomenon flos-aquae was slightly decreased by high contents of SFAs. The present data show that microalgae are rich in valuable nutrients, but the macro- and micronutrient profiles differ strongly between and within species.

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