scholarly journals Progressive loss of DNA sequences from terminal chromosome deficiencies in Drosophila melanogaster.

1988 ◽  
Vol 7 (4) ◽  
pp. 1081-1086 ◽  
Author(s):  
H. Biessmann ◽  
J. M. Mason
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Progressive Loss

Restriction of P-element insertions at the Notch locus of Drosophila melanogaster

1987 ◽  
Vol 7 (4) ◽  
pp. 1545-1548
Author(s):  
M R Kelley ◽  
S Kidd ◽  
R L Berg ◽  
M W Young
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Consensus Sequence ◽  
P Element ◽  
Induced Mutations ◽  
P Elements ◽  
Target Dna ◽  
Complex Locus ◽  
Additional Level ◽  
Derivatives Of

P elements move about the Drosophila melanogaster genome in a nonrandom fashion, preferring some chromosomal targets for insertion over others (J. C. J. Eeken, F. H. Sobels, V. Hyland, and A. P. Schalet, Mutat. Res. 150:261-275, 1985; W. R. Engels, Annu. Rev. Genet. 17:315-344, 1983; M. D. Golubovsky, Y. N. Ivanov, and M. M. Green, Proc. Natl. Acad. Sci. USA 74:2973-2975, 1977; M. J. Simmons and J. K. Lim, Proc. Natl. Acad. Sci. USA 77:6042-6046, 1980). Some of this specificity may be due to recognition of a particular DNA sequence in the target DNA; derivatives of an 8-base-pair consensus sequence are occupied by these transposable elements at many different chromosomal locations (K. O'Hare and G. M. Rubin, Cell 34:25-36, 1983). An additional level of specificity of P-element insertions is described in this paper. Of 14 mutations induced in the complex locus Notch by hybrid dysgenesis, 13 involved P-element insertions at or near the transcription start site of the gene. This clustering was not seen in other transposable element-induced mutations of Notch. DNA sequences homologous to the previously described consensus target for P-element insertion are not preferentially located in this region of the locus. The choice of a chromosomal site for integration appears to be based on more subtle variations in chromosome structure that are probably associated with activation or expression of the target gene.

Satellite DNA sequences of Drosophila melanogaster

1975 ◽  
Vol 96 (4) ◽  
pp. 665-692 ◽  
Author(s):  
Sharyn A. Endow ◽  
Mary Lake Polan ◽  
Joseph G. Gall
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Satellite Dna

scholarly journals Sequences involved in temperature and ecdysterone-induced transcription are located in separate regions of a Drosophila melanogaster heat shock gene.

1986 ◽  
Vol 6 (2) ◽  
pp. 663-673 ◽  
Author(s):  
E Hoffman ◽  
V Corces
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Dna Sequences ◽  
Germ Line ◽  
Initiation Site ◽  
P Element ◽  
Heat Shock Gene ◽  
Base Pairs ◽  
Consensus Sequences ◽  
Shock Gene

The transcriptional regulation of the Drosophila melanogaster hsp27 (also called hsp28) gene was studied by introducing altered genes into the germ line by P element-mediated transformation. DNA sequences upstream of the gene were defined with respect to their effect on steroid hormone-induced and heat-induced transcription. These two types of control were found to be separable; the sequences responsible for 80% of heat-induced expression were located more than 1.1 kilobases upstream of the RNA initiation site, while the sequences responsible for the majority of ecdysterone induction were positioned downstream of the site at -227 base pairs. We have determined the DNA sequence of the intergenic region separating hsp23 and hsp27 and have located putative heat shock and ecdysterone consensus sequences. Our results indicate that the heat shock promoter of the hsp27 gene is organized quite differently from that of hsp70.

A survey of the DNA sequences surrounding the Bari 1 repeats in the pericentromeric h39 region of Drosophila melanogaster

Gene ◽  
2003 ◽  
Vol 307 ◽  
pp. 167-174 ◽  
Author(s):  
Renè Massimiliano Marsano ◽  
Roberta Moschetti ◽  
Paolo Barsanti ◽  
Corrado Caggese ◽  
Ruggiero Caizzi
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences

A genetic and molecular profile of third chromosome centric heterochromatin in Drosophila melanogaster

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 571-584 ◽  
Author(s):  
K A Fitzpatrick ◽  
D A Sinclair ◽  
S R Schulze ◽  
M Syrzycka ◽  
B M Honda
Keyword(s):  
Drosophila Melanogaster ◽  
Genome Sequencing ◽  
Dna Sequences ◽  
Centromeric Heterochromatin ◽  
Molecular Profile ◽  
Chromosome 3 ◽  
Unpublished Work ◽  
Sequencing Studies ◽  
Substantial Progress ◽  
Complementation Groups

In this review, we combine the results of our published and unpublished work with the published results of other laboratories to provide an updated map of the centromeric heterochromatin of chromosome 3 in Drosophila melanogaster. To date, we can identify more than 20 genes (defined DNA sequences with well-characterized functions and (or) defined genetic complementation groups), including at least 16 essential loci. With the ongoing emergence of data from genetic, cytological, and genome sequencing studies, we anticipate continued, substantial progress towards understanding the function, structure, and evolution of centric heterochromatin.Key words: heterochromatin, Drosophila, cytogenetics, genomics.

scholarly journals Molecular cloning of suppressor of sable, a Drosophila melanogaster transposon-mediated suppressor.

1986 ◽  
Vol 6 (5) ◽  
pp. 1520-1528 ◽  
Author(s):  
D Y Chang ◽  
B Wisely ◽  
S M Huang ◽  
R A Voelker
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Insertion Site ◽  
Hybrid Dysgenesis ◽  
P Element ◽  
Dna Transformation ◽  
Wild Type ◽  
Induced Mutations ◽  
X Ray ◽  
Element Insertion

A hybrid dysgenesis-induced allele [su(s)w20] associated with a P-element insertion was used to clone sequences from the su(s) region of Drosophila melanogaster by means of the transposon-tagging technique. Cloned sequences were used to probe restriction enzyme-digested DNAs from 22 other su(s) mutations. None of three X-ray-induced or six ethyl methanesulfonate-induced su(s) mutations possessed detectable variation. Seven spontaneous, four hybrid dysgenesis-induced, and two DNA transformation-induced mutations were associated with insertions within 2.0 kilobases (kb) of the su(s)w20 P-element insertion site. When the region of DNA that included the mutational insertions was used to probe poly(A)+ RNAs, a 5-kb message was detected in wild-type RNA that was present in greatly reduced amounts in two su(s) mutations. By using strand-specific probes, the direction of transcription of the 5-kb message was determined. The mutational insertions lie in DNA sequences near the 5' end of the 5-kb message. Three of the seven spontaneous su(s) mutations are associated with gypsy insertions, but they are not suppressible by su(Hw).

scholarly journals Characterizing dopaminergic neuron vulnerability using Genome-wide analysis

Genetics ◽  
2021 ◽  
Author(s):  
Jacinta Davis ◽  
Claire Da Silva Santos ◽  
Narda Caudillo Zavala ◽  
Nicholas Gans ◽  
Daniel Patracuolla ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Dopaminergic Neuron ◽  
Reference Panel ◽  
Functional Validation ◽  
Mutant Analysis ◽  
Genome Wide Analysis ◽  
Progressive Loss ◽  
Genome Wide ◽  
Drosophila Genetic Reference Panel ◽  
The Brain

Abstract Parkinson’s Disease (PD) is primarily characterized by the loss of dopaminergic (DA) neurons in the brain. However, little is known about why DA neurons are selectively vulnerable to PD. To identify genes that are associated with DA neuron loss, we screened through 201 wild-caught populations of Drosophila melanogaster as part of the Drosophila Genetic Reference Panel (DGRP). Here we identify the top associated genes containing SNPs that render DA neurons vulnerable. These genes were further analyzed by using mutant analysis and tissue-specific knockdown for functional validation. We found that this loss of DA neurons caused progressive locomotor dysfunction in mutants and gene knockdown analysis. The identification of genes associated with the progressive loss of DA neurons should help to uncover factors that render these neurons vulnerable in PD, and possibly develop strategies to make these neurons more resilient.

scholarly journals The organization and expression of the light gene, a heterochromatic gene of Drosophila melanogaster.

Genetics ◽  
1990 ◽  
Vol 125 (1) ◽  
pp. 129-140 ◽  
Author(s):  
R H Devlin ◽  
B Bingham ◽  
B T Wakimoto
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Molecular Mapping ◽  
Single Copy ◽  
Transcription Unit ◽  
Malpighian Tubules ◽  
Centromeric Heterochromatin ◽  
Chromosome 2 ◽  
Induced Mutations ◽  
Flanking Regions

Abstract The light (lt) gene is located in the centromeric heterochromatin of chromosome 2 of Drosophila melanogaster. This gene is necessary for normal levels of pigmentation in a number of adult and larval tissues and is required for viability. Hybrid dysgenic and X-ray induced mutations have been used to identify the gene and compare its organization to that of euchromatic genes. Molecular mapping of lt mutations and its major transcripts has shown that the lt gene is at least 17 kb. By injecting cosmid clones that include this region into lt mutant embryos, we have defined a 30-kb region that can transiently rescue the pigmentation defect in the Malpighian tubules. The major transcription unit of this gene is comprised of exons that are single copy. It is unusual in its organization in having a heterogeneous array of middle repetitive DNA sequences within its intronic and flanking regions.

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