Differentially expressed miRNAs in bone after methotrexate treatment

Background: Drought is one of the most damaging and widespread abiotic stresses that can severely limit the rice production. MicroRNAs (miRNAs) act as a promising tool for improving the drought tolerance of rice and have become a hot spot in recent years. Objective: In order to further extend the understanding of miRNAs, the functions of miRNAs in rice under drought stress are analyzed by bioinformatics. Method: In this study, we integrated miRNAs and genes transcriptome data of rice under the drought stress. Some bioinformatics methods were used to reveal the functions of miRNAs in rice under drought stress. These methods included target genes identification, differentially expressed miRNAs screening, enrichment analysis of DEGs, network constructions for miRNA-target and target-target proteins interaction. Results: (1) A total of 229 miRNAs with differential expression in rice under the drought stress, corresponding to 73 rice miRNAs families, were identiﬁed. (2) 1035 differentially expressed genes (DEGs) were identified, which included 357 up-regulated genes, 542 down-regulated genes and 136 up/down-regulated genes. (3) The network of regulatory relationships between 73 rice miRNAs families and 1035 DEGs was constructed. (4) 25 UP_KEYWORDS terms of DEGs, 125 GO terms and 7 pathways were obtained. (5) The protein-protein interaction network of 1035 DEGs was constructed. Conclusion: (1) MiRNA-regulated targets in rice might mainly involve in a series of basic biological processes and pathways under drought conditions. (2) MiRNAs in rice might play critical roles in Lignin degradation and ABA biosynthesis. (3) MiRNAs in rice might play an important role in drought signal perceiving and transduction.

Download Full-text

miRNA Expression Characterizes Histological Subtypes and Metastasis in Penile Squamous Cell Carcinoma

Cancers ◽

10.3390/cancers13061480 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1480

Author(s):

Hiresh Ayoubian ◽

Joana Heinzelmann ◽

Sebastian Hölters ◽

Oybek Khalmurzaev ◽

Alexey Pryalukhin ◽

...

Keyword(s):

Microarray Data ◽

Expression Patterns ◽

Hpv Infection ◽

Differentially Expressed ◽

Histological Subtypes ◽

Specific Expression ◽

Tissue Samples ◽

Qrt Pcr ◽

Differentially Expressed Mirnas ◽

The Impact

Although microRNAs are described as promising biomarkers in many tumor types, little is known about their role in PSCC. Thus, we attempted to identify miRNAs involved in tumor development and metastasis in distinct histological subtypes considering the impact of HPV infection. In a first step, microarray analyses were performed on RNA from formalin-fixed, paraffin-embedded tumor (22), and normal (8) tissue samples. Microarray data were validated for selected miRNAs by qRT-PCR on an enlarged cohort, including 27 tumor and 18 normal tissues. We found 876 significantly differentially expressed miRNAs (p ≤ 0.01) between HPV-positive and HPV-negative tumor samples by microarray analysis. Although no significant differences were detected between normal and tumor tissue in the whole cohort, specific expression patterns occurred in distinct histological subtypes, such as HPV-negative usual PSCC (95 differentially expressed miRNAs, p ≤ 0.05) and HPV-positive basaloid/warty subtypes (247 differentially expressed miRNAs, p ≤ 0.05). Selected miRNAs were confirmed by qRT-PCR. Furthermore, microarray data revealed 118 miRNAs (p ≤ 0.01) that were significantly differentially expressed in metastatic versus non-metastatic usual PSCC. The lower expression levels for miR-137 and miR-328-3p in metastatic usual PSCC were validated by qRT-PCR. The results of this study confirmed that specific miRNAs could serve as potential diagnostic and prognostic markers in single PSCC subtypes and are associated with HPV-dependent pathways.

Download Full-text

MicroRNA expression profile in bovine mammary gland parenchyma infected by coagulase-positive or coagulase-negative staphylococci

Veterinary Research ◽

10.1186/s13567-021-00912-2 ◽

2021 ◽

Vol 52 (1) ◽

Author(s):

Emilia Bagnicka ◽

Ewelina Kawecka-Grochocka ◽

Klaudia Pawlina-Tyszko ◽

Magdalena Zalewska ◽

Aleksandra Kapusta ◽

...

Keyword(s):

Mammary Gland ◽

Immune System ◽

Differentially Expressed ◽

Bacterial Invasion ◽

Coagulase Negative Staphylococci ◽

Cellular Processes ◽

Differentially Expressed Mirnas ◽

Non Coding Rnas ◽

Coagulase Positive Staphylococci ◽

Generation Sequencing

AbstractMicroRNAs (miRNAs) are short, non-coding RNAs, 21–23 nucleotides in length which are known to regulate biological processes that greatly impact immune system activity. The aim of the study was to compare the miRNA expression in non-infected (H) mammary gland parenchyma samples with that of glands infected with coagulase-positive staphylococci (CoPS) or coagulase-negative staphylococci (CoNS) using next-generation sequencing. The miRNA profile of the parenchyma was found to change during mastitis, with its profile depending on the type of pathogen. Comparing the CoPS and H groups, 256 known and 260 potentially new miRNAs were identified, including 32 that were differentially expressed (p ≤ 0.05), of which 27 were upregulated and 5 downregulated. Comparing the CoNS and H groups, 242 known and 171 new unique miRNAs were identified: 10 were upregulated (p ≤ 0.05), and 2 downregulated (p ≤ 0.05). In addition, comparing CoPS with H and CoNS with H, 5 Kyoto Encyclopedia of Genes and Genomes pathways were identified; in both comparisons, differentially-expressed miRNAs were associated with the bacterial invasion of epithelial cells and focal adhesion pathways. Four gene ontology terms were identified in each comparison, with 2 being common to both immune system processes and signal transduction. Our results indicate that miRNAs, especially miR-99 and miR-182, play an essential role in the epigenetic regulation of a range of cellular processes, including immunological systems bacterial growth in dendritic cells and disease pathogenesis (miR-99), DNA repair and tumor progression (miR-182).

Download Full-text

Differentially expressed miRNAs in trisomy 21 placentas

Prenatal Diagnosis ◽

10.1002/pd.4861 ◽

2016 ◽

Vol 36 (8) ◽

pp. 775-784 ◽

Cited By ~ 8

Author(s):

Iveta Svobodová ◽

Marie Korabečná ◽

Pavel Calda ◽

Miroslav Břešťák ◽

Eva Pazourková ◽

...

Keyword(s):

Trisomy 21 ◽

Differentially Expressed ◽

Differentially Expressed Mirnas

Download Full-text

Identification of differentially expressed miRNAs in chicken lung and trachea with avian influenza virus infection by a deep sequencing approach

BMC Genomics ◽

10.1186/1471-2164-10-512 ◽

2009 ◽

Vol 10 (1) ◽

pp. 512 ◽

Cited By ~ 77

Author(s):

Ying Wang ◽

Vinayak Brahmakshatriya ◽

Huifeng Zhu ◽

Blanca Lupiani ◽

Sanjay M Reddy ◽

...

Keyword(s):

Influenza Virus ◽

Virus Infection ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Deep Sequencing ◽

Influenza Virus Infection ◽

Differentially Expressed ◽

Differentially Expressed Mirnas ◽

Deep Sequencing Approach

Download Full-text

MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells

Food & Function ◽

10.1039/d1fo01260f ◽

2021 ◽

Author(s):

Zhiyun Hao ◽

Yuzhu Luo ◽

Jiqing Wang ◽

Jon Hickford ◽

Huitong Zhou ◽

...

Keyword(s):

Mammary Gland ◽

Epithelial Cells ◽

Milk Production ◽

Milk Fat ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

Differentially Expressed ◽

Differentially Expressed Mirnas ◽

Fat Synthesis ◽

Milk Fat Synthesis

In our previous studies, microRNA-432 (miR-432) was found to be one of differentially expressed miRNAs in ovine mammary gland between the two breeds of lactating sheep with different milk production...

Download Full-text

Identification of differentially expressed microRNAs in outgrowth embryos compared with blastocysts and non-outgrowth embryos in mice

Reproduction Fertility and Development ◽

10.1071/rd18161 ◽

2019 ◽

Vol 31 (4) ◽

pp. 645 ◽

Cited By ~ 6

Author(s):

Jihyun Kim ◽

Jaewang Lee ◽

Jin Hyun Jun

Keyword(s):

Bioinformatics Analysis ◽

Preimplantation Embryo ◽

Expression Profiles ◽

In Silico Analysis ◽

Differentially Expressed ◽

Recurrent Implantation Failure ◽

Preimplantation Embryo Development ◽

Mirna Expression Profiles ◽

Differentially Expressed Mirnas ◽

Silico Analysis

Recurrent implantation failure (RIF) is one of the main causes for the repeated failure of IVF, and the major reason for RIF is thought to be a miscommunication between the embryo and uterus. However, the exact mechanism underlying embryo–uterus cross-talk is not fully understood. The aim of the present study was to identify differentially expressed microRNAs (miRNAs) among blastocysts, non-outgrowth and outgrowth embryos in mice using microarray analysis. A bioinformatics analysis was performed to predict the potential mechanisms of implantation. The miRNA expression profiles differed significantly between non-outgrowth and outgrowth embryos. In all, 3163 miRNAs were detected in blastocysts and outgrowth embryos. Of these, 10 miRNA candidates (let-7b, miR-23a, miR-27a, miR-92a, miR-183, miR-200c, miR-291a, miR-425, miR-429 and miR-652) were identified as significant differentially expressed miRNAs of outgrowth embryos by in silico analysis. The expression of the miRNA candidates was markedly changed during preimplantation embryo development. In particular, let-7b-5p, miR-200c-3p and miR-23a-3p were significantly upregulated in outgrowth embryos compared with non-outgrowth blastocysts. Overall, differentially expressed miRNAs in outgrowth embryos compared with blastocysts and non-outgrowth embryos could be involved in embryo attachment, and interaction between the embryo proper and maternal endometrium during the implantation process.

Download Full-text

MicroRNA sequence analysis identifies microRNAs associated with peri-implantitis in dogs

Bioscience Reports ◽

10.1042/bsr20170768 ◽

2017 ◽

Vol 37 (5) ◽

Cited By ~ 10

Author(s):

Xiaolin Wu ◽

Xipeng Chen ◽

Wenxiang Mi ◽

Tingting Wu ◽

Qinhua Gu ◽

...

Keyword(s):

Sequence Analysis ◽

Target Genes ◽

Alveolar Bone ◽

Biological Effects ◽

Bone Destruction ◽

Mapk Signaling ◽

Differentially Expressed ◽

Mitogen Activated Protein Kinase ◽

Mirna Sequence ◽

Differentially Expressed Mirnas

Peri-implantitis, which is characterized by dense inflammatory infiltrates and increased osteoclast activity, can lead to alveolar bone destruction and implantation failure. miRNAs participate in the regulation of various inflammatory diseases, such as periodontitis and osteoporosis. Therefore, the present study aimed to investigate the differential expression of miRNAs in canine peri-implantitis and to explore the functions of their target genes. An miRNA sequence analysis was used to identify differentially expressed miRNAs in peri-implantitis. Under the criteria of a fold-change >1.5 and P<0.01, 8 up-regulated and 30 down-regulated miRNAs were selected for predictions of target genes and their biological functions. Based on the results of Gene Ontology (GO) and KEGG pathway analyses, these miRNAs may fine-tune the inflammatory process in peri-implantitis through an intricate mechanism. The results of quantitative real-time PCR (qRT-PCR) revealed that let-7g, miR-27a, and miR-145 may play important roles in peri-implantitis and are worth further investigation. The results of the present study provide insights into the potential biological effects of the differentially expressed miRNAs, and specific enrichment of target genes involved in the mitogen-activated protein kinase (MAPK) signaling pathway was observed. These findings highlight the intricate and specific roles of miRNAs in inflammation and osteoclastogenesis, both of which are key aspects of peri-implantitis, and thus may contribute to future investigations of the etiology, underlying mechanism, and treatment of peri-implantitis.

Download Full-text