Bimetal oxalate‐derived synthesis of laponite‐decorated CoFe 2 O 4 porous nanostructures for activation of peroxymonopersulfate towards degradation of Rhodamine B

Biosorpsi zat warna Rhodamine B menggunakan cangkang Pensi (Corbicula moltkiana) telah dikaji. Percobaan dilakukan dengan system batch guna memperoleh kondisi optimum biosorspi zat warna. Kapasitas biosorpsi zat warna pada pH 2 adalah 0.9958 mg/g, dengan konsentrasi larutan mula-mula 150 mg/L waktu kontak 105 menit, massa biosorben 0.1 g, ukuran partikel 32 µm dan temperature pengeringan biosorben pada 75oC. Model isotherm Langmuir menunjukkan bahwa proses penyerapan berlangsung secara kimia dan biosorpsi homogeny dari adsorbat (Rhodamine B) pada permukaan biosorben membentuk lapisan tunggal dengan nilai R2 0.9966. Analisis XRF menunjukkan bahwa penurunan kadar unsur logam pada cangkang Pensi membuktikan bahwa proses biosorpsi berlangsung dengan pertukaran kation. Hasil analisis spektrum FT-IR membuktikan adanya interaksi antaramolekul Rhodamin B dengan gugus fungsi pada cangkang Pensi. Analisis dengan SEM memperlihatkan bahwa pori-pori cangkang Pensi telah terisi penuh oleh molekul Rhodamin B. Kondisi optimum biosorpsi telah diaplikasikan pada limbah kerupuk merah dengan kapasitas penyerapan sebesar 0,2835 mg/g. The biosorption of Rhodamine B dyes by Pensi (Corbicula moltkiana) shell has been investigated. The experiment was conducted in batch sistem in order to obtain the optimum conditions of dye biosorption. Biosorption capacity of dye was 0.9958 mg/g at pH 2, initial concentration 150 mg/L, contact time 105 minutes, biosorbent mass 0.1 gram, particle size 32 µ m and biosorbent drying temperature was at 75oC. The Langmuir Isotherm model showed chemisorption and homogeneous biosorption process of adsorbates onto the biosorbent surface formed monolayer dye molecules on the biosorbent surface with R2 value was 0.9966. XRF analysis showed that reduction of metals unsure quantity of pensi shell indicated biosorption process was occupied through cationic exchange. The result of FTIR spectra analysis indicated an interaction between Rhodamin B molecules and functional group of pensi shell. SEM analysis showed that the pensi shell pores were completely filled by Rhodamine B molecules. The optimum condition of biosorption has been aplicated in red chips wastewater industry with biosorption capacity was 0.2835 mg/g.

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Using FRET to Measure the Time it Takes for a Cell to Destroy a Virus

10.26434/chemrxiv.10299164.v1 ◽

2019 ◽

Author(s):

Candace E. Benjamin ◽

Zhuo Chen ◽

Olivia Brohlin ◽

Hamilton Lee ◽

Stefanie Boyd ◽

...

Keyword(s):

Cellular Uptake ◽

Rhodamine B ◽

Virus Like Particle ◽

A Cell ◽

Viral Nanoparticles ◽

The Stability ◽

Open Question ◽

Viral Surface ◽

Fret Pair

<div><div><div><p>The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, the in vitro fate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Qβ - a representative and popular VLP for several applications - following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle’s behavior in vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.</p></div></div></div>

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KEJADIAN LUAR BIASA KERACUNAN “CUMI-CUMIAN” DI SEKOLAH DASAR NEGERI 1 TRASAN BANDONGAN KABUPATEN MAGELANG

Media Ilmu Kesehatan ◽

10.30989/mik.v7i2.280 ◽

2018 ◽

Vol 7 (2) ◽

pp. 131-136

Author(s):

Nasir Ahmad

Keyword(s):

Abdominal Pain ◽

Bacillus Cereus ◽

Rhodamine B ◽

Attack Rate ◽

Food Poisoning ◽

Health Department ◽

Analysis Data ◽

Food Samples ◽

Bivariate Analysis ◽

School Committee

Background: On May 4th, 2016, at 12:30 district surveillance officer of Magelang Health Department received reports from Public Health Center of Bandongan about 21 students of SDN 1 Trasan who suffered from the same food-poisoning symptoms. Objective: Investigation was carried out to identify the source, how it spread and how to control it. Methods: This study used descriptive analytic and mapping the cases distribution location. The case was people experiencing symptoms of dizziness or abdominal pain or nausea or vomiting. Data analysis was done by using bivariate analysis. Data collection were done through interviews, observations and laboratory tests on the food samples. Results: The case was 50 students (from 1-6 grade students). The perceived symptoms were dizziness (77%), nausea (42%), abdominal pain (40%) and vomiting (8%). Attack rate found ranged from 14.3% to 60% with the highest Attack rate found on class three (60%). The incubation period of 15-240 minutes (mean 72.3 minutes). Calamari like positive Bacillus cereus and Rhodamine-B 10 mg/kg. Conclusion: The outbreak of food poisoning because calamari like contaminated Bacillus cereus. We suggested the school committee to provide the socialization of harmful food for the students. The teachers should restrict the permission for the food vendor to sell at school. Keywords: Bacillus cereus, , Food Poisoning, Outbreak, Rhodamine B, School Food

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Estudios de dispersión de contaminantes y declinación bacteriana en la bahía del mar de Chimbote - Perú

Perfiles de Ingeniería ◽

10.31381/perfiles_ingenieria.v1i10.440 ◽

2016 ◽

Vol 1 (10) ◽

Author(s):

Ing. Carlos Sebastián Calvo

Keyword(s):

Rhodamine B ◽

Iodine 131 ◽

Para Determinar

Una revisión de los estudios de dispersión y una determinación preliminar de T-90 se ha realizado en el mar de Chimbote-Perú. En relación a los estudios de dispersión se presenta una ecuación diferencial fundamental y su solución para el caso de tres dimensiones. La forma y características de la pluma trazadora se definen también como el ratio de disminución de las concentraciones pico. El método considera un punto de inyección con Rhodamine B y Iodine 131 como trazadoras para determinar los coeficientes de dispersión que gobiernas los ratios de dispersión de aguas industriales y desecho a ser descargadas a través de una salida que va a ser instalada en el mar de Chimbote. El método presentado aquí permite el cálculo de los coeficientes de dispersión e influencia del difusor sin importar la naturaleza de la composición del agua en los cuales la prueba se lleva a cabo. Con relación las determinaciones T-90, ellos tuvieron mejor desempeño por el uso de una técnica trazadora combinada, usando el I-131 para la medida de la dilución y Rhodamine B como una ayuda visual. Se tomaron en consideración la dilución y decrecimiento en las concentraciones bacteriales al igual que la inyección continua de radio trazadores. Los valores de T-90 estimados desde los experimentos deberían ser reconfirmados en futuros experimentos bajo condiciones oceanográficas diferentes.

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Faculty Opinions recommendation of Reagent for the detection of protein thiocarboxylates in the bacterial proteome: lissamine rhodamine B sulfonyl azide.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5139957.5076055 ◽

2010 ◽

Author(s):

Marc Fontecave ◽

Sandrine Ollagnier de Choudens

Keyword(s):

Rhodamine B ◽

Sulfonyl Azide

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Preparation of AgCl/Ag3PO4/Diatomite Composite by Microemulsion Method for Rapid Photo-Degradation of Rhodamine B with Stability under Visible Light

Korean Journal of Materials Research ◽

10.3740/mrsk.2020.30.8.383 ◽

2020 ◽

Vol 30 (8) ◽

pp. 383-392

Author(s):

Hai-Tao Zhu ◽

◽

Qi-Fang Ren ◽

Zhen Jin ◽

Yi Ding ◽

...

Keyword(s):

Visible Light ◽

Rhodamine B ◽

Photo Degradation ◽

Microemulsion Method

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Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

Protein and Peptide Letters ◽

10.2174/0929866526666190619110430 ◽

2019 ◽

Vol 26 (10) ◽

pp. 758-767

Author(s):

Vicente Rubio ◽

Vijaya Iragavarapu ◽

Maciej J. Stawikowski

Keyword(s):

Quantum Yield ◽

Cancer Cells ◽

Fluorescent Probe ◽

Extinction Coefficient ◽

Rhodamine B ◽

Solid Phase ◽

Total Yield ◽

Molar Extinction Coefficient ◽

Αvβ3 Integrin ◽

Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

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