In vivo antioxidant potential of Sweet chestnut (Castanea sativa Mill.) wood extract in young growing pigs exposed to n -3 PUFA-induced oxidative stress

2011 ◽  
Vol 91 (8) ◽  
pp. 1432-1439 ◽  
Author(s):  
Tamara Frankič ◽  
Janez Salobir
Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1538
Author(s):  
Ana Teresa Alhinho ◽  
Miguel Jesus Nunes Ramos ◽  
Sofia Alves ◽  
Margarida Rocheta ◽  
Leonor Morais-Cecílio ◽  
...  

The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecular mechanisms involved in the determination of sexual organ identity. Thus, the study of how the different flowers of C. sativa develop is fundamental to understand the reproductive success of this species and the impact of flower phenology on its productivity. In this study, a C. sativa de novo transcriptome was assembled and the homologous genes to those of the ABCDE model for floral organ identity were identified. Expression analysis showed that the C. sativa B- and C-class genes are differentially expressed in the male flowers and female flowers. Yeast two-hybrid analysis also suggested that changes in the canonical ABCDE protein–protein interactions may underlie the mechanisms necessary to the development of separate male and female flowers, as reported for the monoecious Fagaceae Quercus suber. The results here depicted constitute a step towards the understanding of the molecular mechanisms involved in unisexual flower development in C. sativa, also suggesting that the ABCDE model for flower organ identity may be molecularly conserved in the predominantly monoecious Fagaceae family.


2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Nadia Afsheen ◽  
Khalil-ur-Rehman ◽  
Nazish Jahan ◽  
Misbah Ijaz ◽  
Asad Manzoor ◽  
...  

In this research work, the antioxidant and metabolomic profiling of seven selected medicinally important herbs including Rauvolfia serpentina, Terminalia arjuna, Coriandrum sativum, Elettaria cardamom, Piper nigrum, Allium sativum, and Crataegus oxyacantha was performed. The in vivo cardioprotective potential of these medicinal plants was evaluated against surgically induced oxidative stress through left anterior descending coronary artery ligation (LADCA) in dogs. The antioxidant profiling of these plants was done through DPPH and DNA protection assay. The C. oxyacantha and T. arjuna showed maximum antioxidant potential, while the E. cardamom showed poor antioxidative strength even at its high concentration. Different concentrations of extracts of the said plants exhibited the protection of plasmid DNA against H2O2 damage as compared to the plasmid DNA merely treated with H2O2. The metabolomic profiling through LC-MS analysis of these antioxidants revealed the presence of active secondary metabolites responsible for their antioxidant potential. During in vivo analysis, blood samples of all treatment groups were drawn at different time intervals to analyze the cardiac and hemodynamic parameters. The results depicted that the group pretreated with HC4 significantly sustained the level of CK-MB, SGOT, and LDH as well as hemodynamic parameters near to normal. The histopathological examination also confirmed the cardioprotective potential of HC4. Thus, the HC4 being safe and inexpensive cardioprotective herbal combination could be considered as an alternate of synthetic drugs.


2020 ◽  
Vol 10 (20) ◽  
pp. 11192-11216
Author(s):  
Paola Pollegioni ◽  
Stefano Del Lungo ◽  
Ruth Müller ◽  
Keith E. Woeste ◽  
Francesca Chiocchini ◽  
...  

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Margherita Campo ◽  
Patrizia Pinelli ◽  
Annalisa Romani

Sweet Chestnut ( Castanea sativa Mill.) wood extracts, rich in Hydrolyzable Tannins (HTs), are traditionally used in the tanning and textile industries, but recent studies suggest additional uses. The aim of this work is the HPLC-DAD-ESI-MS characterization of Sweet Chestnut aqueous extracts and fractions obtained through a membrane separation technology system without using other solvents, and the evaluation of their antioxidant and antiradical activities. Total tannins range between 2.7 and 138.4 mM; gallic acid ranges between 6% and 100%; castalagin and vescalagin range between 0% and 40%. Gallic Acid Equivalents, measured with the Folin-Ciocalteu test, range between 0.067 and 56.99g/100g extract weight; ORAC test results for the marketed fractions are 450.4 and 3050 μmol/g Trolox Equivalents/extract weight. EC50 values, measured with the DPPH test, range between 0.444 and 2.399 μM. These results suggest a new ecofriendly and economically sustainable method for obtaining chestnut fractions with differentiated, stable and reproducible chemical compositions. Such fractions can be marketed for innovative uses in several sectors.


2019 ◽  
Vol 143 (9-10) ◽  
pp. 469-477
Author(s):  
Damird Drvodelić ◽  
Igor Poljak ◽  
Marilena Idžojtić ◽  
Ivana Zegnal ◽  
Katarina Tumpa ◽  
...  

U radu su prikazani rezultati ispitivanja laboratorijske klijavosti i morfoloških značajki klijanaca europskoga pitomoga kestena (Castanea sativa Mill.). Uzorci za istraživanja sakupljeni su u submediteranskom području Hrvatske, a radni uzorak za ispitivanje klijavosti iznosio je 8×25 sjemenki. Sjeme je do ispitivanja klijavosti čuvano tri mjeseca u hladnjaku na temperaturi od 3 °C. Postotak laboratorijske klijavosti utvrđen je prema postotku pravilnih klijanaca koji su normalno proklijali nakon 27 dana ispitivanja. Ispitivanje je provedeno u laboratoriju prema uvjetima koje propisuje ISTA (International Seed Testing Association). Izrađen je katalog sa slikama i opisom nepravilnih klijanaca. Na osnovi definiranih kodova nepravilnih klijanaca moguće je preventivno djelovati kako bi se reducirale nepoželjne jedinke. Rezultati ovog istraživanja od velike su koristi za razumijevanje složenoga procesa klijanja sjemena, kako u laboratoriju, tako i šumskim rasadnicima.


2011 ◽  
Vol 18 (1) ◽  
pp. 1-5 ◽  
Author(s):  
B.S. Ashok Kumar ◽  
K. Lakshman ◽  
R. Nandeesh ◽  
P.A. Arun Kumar ◽  
B. Manoj ◽  
...  

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