Simulation of Equilibrium and Dynamic Properties of Large DNA Molecules

2006 ◽  
pp. 579-604 ◽  
Author(s):  
Alexander Vologodskii
Keyword(s):  
Dynamic Properties ◽  
Dna Molecules

Replication of Bacteriophage 186 DNA

1972 ◽  
Vol 30 ◽  
pp. 194-195
Author(s):  
Dhruba K. Chattoraj ◽  
Ross B. Inman
Keyword(s):  
Electron Microscopy ◽  
Dna Replication ◽  
Frame Of Reference ◽  
Dna Molecules ◽  
E Coli ◽  
Phage Dna ◽  
Partial Denaturation

Electron microscopy of replicating intermediates has been quite useful in understanding the mechanism of DNA replication in DNA molecules of bacteriophage, mitochondria and plasmids. The use of partial denaturation mapping has made the tool more powerful by providing a frame of reference by which the position of the replicating forks in bacteriophage DNA can be determined on the circular replicating molecules. This provided an easy means to find the origin and direction of replication in λ and P2 phage DNA molecules. DNA of temperate E. coli phage 186 was found to have an unique denaturation map and encouraged us to look into its mode of replication.

The distribution of antigen on the surface of RBL-2H3 rat basophilic leukemia cells observed by back-scattered electron imaging

1986 ◽  
Vol 44 ◽  
pp. 274-275
Author(s):  
R.F. Stump ◽  
J.R. Pfeiffer ◽  
JC. Seagrave ◽  
D. Huskisson ◽  
J.M. Oliver
Keyword(s):  
Cell Surface ◽  
Dynamic Properties ◽  
Leukemia Cells ◽  
Antigen Binding ◽  
Scattered Electron ◽  
Ige Receptor ◽  
Receptor Complexes ◽  
Rat Basophilic Leukemia Cells ◽  
Electron Imaging ◽  
Basophilic Leukemia

In RBL-2H3 rat basophilic leukemia cells, antigen binding to cell surface IgE-receptor complexes stimulates the release of inflammatory mediators and initiates a series of membrane and cytoskeletal events including a transformation of the cell surface from a microvillous to a lamellar topography. It is likely that dynamic properties of the IgE receptor contribute to the activation of these responses. Fewtrell and Metzger have established that limited crosslinking of IgE-receptor complexes is essential to trigger secretion. In addition, Baird and colleagues have reported that antigen binding causes a rapid immobilization of IgE-receptor complexes, and we have demonstrated an apparent increase with time in the affinity of IgE-receptor complexes for antigen.

Synchrony of Digestion of SV40 DNA by Exonuclease III Determined by EM

1975 ◽  
Vol 33 ◽  
pp. 674-675
Author(s):  
Ray Wu ◽  
G. Ruben ◽  
B. Siegel ◽  
P. Spielman ◽  
E. Jay
Keyword(s):  
Dark Field ◽  
Uranyl Acetate ◽  
Enzyme Digestion ◽  
Dna Molecules ◽  
Exonuclease Iii ◽  
Aluminum Films ◽  
Double Stranded Dna ◽  
Before And After ◽  
Exo Iii ◽  
Sv40 Dna

A method for determining long nucleotide sequences of double-stranded DNA is being developed. It involves (a) the synchronous digestion of the DNA from the 3' ends with EL coli exonuclease III (Exo III) followed by (b) resynthesis with labeled nucleotides and DNA polymerase. A crucial factor in the success of this method is the degree to which the enzyme digestion proceeds synchronously under proper conditions of incubation (step a). Dark field EM is used to obtain accurate measurements on the lengths and distribution of the DNA molecules before and after digestion with Exo III, while gel electrophoresis is used in parallel to obtain a mean length for these molecules. It is the measurements on a large enough sample of individual molecules by EM that provides the information on how synchronously the digestion proceeds. For length measurements, the DNA molecules were picked up on 20-30 Å thick carbon-aluminum films, using the aqueous Kleinschmidt technique and stained with 7.5 x 10-5M uranyl acetate in 90% ethanol for 3 minutes.

Nucleosome dynamics

2006 ◽  
Vol 73 ◽  
pp. 109-119 ◽  
Author(s):  
Chris Stockdale ◽  
Michael Bruno ◽  
Helder Ferreira ◽  
Elisa Garcia-Wilson ◽  
Nicola Wiechens ◽  
...  
Keyword(s):  
High Resolution ◽  
Chromatin Structure ◽  
Current Knowledge ◽  
Dynamic Properties ◽  
Structure And Dynamics ◽  
Nucleosome Dynamics ◽  
Sharp Focus ◽  
Recent Advances ◽  
Insight Into ◽  
Chromatin Structure And Dynamics

In the 30 years since the discovery of the nucleosome, our picture of it has come into sharp focus. The recent high-resolution structures have provided a wealth of insight into the function of the nucleosome, but they are inherently static. Our current knowledge of how nucleosomes can be reconfigured dynamically is at a much earlier stage. Here, recent advances in the understanding of chromatin structure and dynamics are highlighted. The ways in which different modes of nucleosome reconfiguration are likely to influence each other are discussed, and some of the factors likely to regulate the dynamic properties of nucleosomes are considered.

NMR-investigation of the dynamic properties of off-center Ag+ defects in RbCl

1980 ◽  
Vol 41 (C6) ◽  
pp. C6-404-C6-407 ◽  
Author(s):  
O. Kanert ◽  
R. Küchler ◽  
M. Mali
Keyword(s):  
Dynamic Properties

DYNAMIC PROPERTIES OF GRAIN BOUNDARIES

1990 ◽  
Vol 51 (C1) ◽  
pp. C1-381-C1-390 ◽  
Author(s):  
M. FRIESEL ◽  
I. MANNA ◽  
W. GUST
Keyword(s):  
Grain Boundaries ◽  
Dynamic Properties

KINEMATIC FEATURES OF THE LAPPING PROCESS AND DETERMINATION OF ITS BASIC PARAMETERS

2020 ◽  
Vol 7 (3) ◽  
pp. 23-28
Author(s):  
EZIZ SARVAN SHIRVAN ◽  
Keyword(s):  
Dynamic Properties ◽  
Cutting Speed ◽  
Equilibrium System ◽  
Technological Parameters ◽  
Kinematic Characteristics ◽  
Angular Velocities ◽  
Basic Parameters ◽  
Grinding Tool ◽  
Mathematical Relationships ◽  
Analytical Expressions

This paper discusses the kinematic characteristics of lapping process and the main parameters of the process. It was determined that the influencing degree of technological parameters to the forming surface and processes. It was projected the construction of the lapping head for processing of internal cylindrical surfaces, scheme of the lapping operation and graphic description of the forces influencing. The relationships between the axial, radial and tangential cutting forces and the effect of the combined force thereof are determined in order to ensure the necessary surface pressure. During the analysis geometric and mathematical relationships were obtained. The extracted analytical expressions can be realized by further experimental researches and can be used in engineering calculations of technological parameters of processing by lapping. Angular velocity, friction force, linear velocity, also the length of the tactile curve and the radius of the part can be considered the main kinematic and dynamic parameters of the process that the formation of the surface, also the course of the process depends on these parameters. Depending on the kinematic parameters, the wear nature of the tool changes and this changes the linear and angular velocities, which have a significant impact on the accuracy, quality and productivity of processing. When examining the technological capabilities of the process, the nature of the movement between the part and the grinding tool, also changes in cutting speed are often considered as a main factor. Analytical expressions were obtained to determine the main parameters of the process, taking into account the kinematic characteristics of the friction process. These expressions can be used in engineering calculations and allow to determine the optimal values of the processing mode. In order to obtain the required micrometric surface cleanliness and measurement accuracy, correlation relationships were established between the main parameters of the process, equations of the equilibrium system of shear forces were compiled and analytical expressions were obtained based on the analysis of kinematic and dynamic properties of the system.

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