Rational Approaches to Antibacterial Discovery: Pre-Genomic Directed and Phenotypic Screening

2011 ◽  
pp. 33-75 ◽  
Author(s):  
Lynn L. Silver
Keyword(s):  
Phenotypic Screening

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

Phenotypic screening with target identification and validation in the discovery and development of E3 ligase modulators

2021 ◽  
Vol 28 (3) ◽  
pp. 283-299
Author(s):  
Nil Ege ◽  
Habib Bouguenina ◽  
Marianthi Tatari ◽  
Rajesh Chopra
Keyword(s):  
Target Identification ◽  
E3 Ligase ◽  
Phenotypic Screening

scholarly journals Phenotypic screening, transcriptional profiling, and comparative genomic analysis of an invasive and non-invasive strain of Candida albicans

2008 ◽  
Vol 8 (1) ◽  
pp. 187 ◽  
Author(s):  
Sascha Thewes ◽  
Gary P Moran ◽  
Beatrice B Magee ◽  
Martin Schaller ◽  
Derek J Sullivan ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Transcriptional Profiling ◽  
Genomic Analysis ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
Phenotypic Screening ◽  
Non Invasive ◽  
Invasive Strain

scholarly journals High-Content Analysis to Leverage a Robust Phenotypic Profiling Approach to Vascular Modulation

2013 ◽  
Vol 18 (10) ◽  
pp. 1246-1259 ◽  
Author(s):  
Beverley J. Isherwood ◽  
Rebecca E. Walls ◽  
Mark E. Roberts ◽  
Thomas M. Houslay ◽  
Sandra R. Brave ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Content Analysis ◽  
Cell Biology ◽  
High Volume ◽  
Phenotypic Screening ◽  
Stromal Fibroblasts ◽  
Physiological Relevance ◽  
Automated Microscopy ◽  
High Content Analysis ◽  
Phenotypic Responses

Phenotypic screening seeks to identify substances that modulate phenotypes in a desired manner with the aim of progressing first-in-class agents. Successful campaigns require physiological relevance, robust screening, and an ability to deconvolute perturbed pathways. High-content analysis (HCA) is increasingly used in cell biology and offers one approach to prosecution of phenotypic screens, but challenges exist in exploitation where data generated are high volume and complex. We combine development of an organotypic model with novel HCA tools to map phenotypic responses to pharmacological perturbations. We describe implementation for angiogenesis, a process that has long been a focus for therapeutic intervention but has lacked robust models that recapitulate more completely mechanisms involved. The study used human primary endothelial cells in co-culture with stromal fibroblasts to model multiple aspects of angiogenic signaling: cell interactions, proliferation, migration, and differentiation. Multiple quantitative descriptors were derived from automated microscopy using custom-designed algorithms. Data were extracted using a bespoke informatics platform that integrates processing, statistics, and feature display into a streamlined workflow for building and interrogating fingerprints. Ninety compounds were characterized, defining mode of action by phenotype. Our approach for assessing phenotypic outcomes in complex assay models is robust and capable of supporting a range of phenotypic screens at scale.

Ex Vivo Phenotypic Screening of Two Small Repurposing Drug Collections Identifies Nifuratel as a Potential New Treatment against Visceral and Cutaneous Leishmaniasis

2021 ◽  
Author(s):  
Bárbara Domínguez-Asenjo ◽  
Camino Gutiérrez-Corbo ◽  
María Álvarez-Bardón ◽  
Yolanda Pérez-Pertejo ◽  
Rafael Balaña-Fouce ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Ex Vivo ◽  
Phenotypic Screening ◽  
New Treatment

scholarly journals An Expedited Phenotypic Approach Towards Organic Reaction Generality

2021 ◽  
Author(s):  
Cesar N. Prieto Kullmer ◽  
Jacob A. Kautzky ◽  
Shane W. Krska ◽  
Timothy Nowak ◽  
Spencer D. Dreher ◽  
...  
Keyword(s):  
Synthetic Methods ◽  
Phenotypic Screening ◽  
Organic Reaction

Reaction generality is crucial in determining the overall impact and usefulness of organic synthetic methods. In contrast, contemporary generalization processes seem unable to meet the growing demand for robust methodology. We sought to develop an accelerated approach towards achieving generality, inspired by phenotypic screening, that rapidly expands the scope and utility of synthetic methods. This approach was validated by example of the metallaphotoredox decarboxylative arylation, resulting in the discovery of a novel additive that overcomes many lingering limitations of this method and has significant mechanistic implications for nickel-catalyzed cross couplings in general.

scholarly journals High Throughput Phenotypic Screening of The Human Spermatozoon

Reproduction ◽  
2021 ◽  
Author(s):  
Zoe Claire Johnston ◽  
Franz S Gruber ◽  
Sean Brown ◽  
Neil R Norcross ◽  
Jason R Swedlow ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Large Scale ◽  
Hormonal Contraceptives ◽  
Diagnostic Tools ◽  
Sperm Function ◽  
Phenotypic Screening ◽  
Environmental Chemical ◽  
Potential Applications

Despite recent advances in male reproductive health research, there remain many elements of male (in)fertility where our understanding is incomplete. Consequently, diagnostic tools and treatments for men with sperm dysfunction, other than medically assisted reproduction, are limited. On the other hand, the gaps in our knowledge of the mechanisms which underpin sperm function have hampered the development of male non-hormonal contraceptives. The study of mature spermatozoa is inherently difficult. They are a unique and highly specialised cell type which does not actively transcribe or translate proteins and cannot be cultured for long periods of time or matured in vitro. One, large scale, approach to both increasing understanding of sperm function, and the discovery and development of compounds that can modulate sperm function, is to directly observe responses to compounds with phenotypic screening techniques. These target agnostic approaches can be developed into high-throughput screening platforms with the potential to drastically increase advances in the field. Here we discuss the rationale and development of high-throughput phenotypic screening platforms for mature human spermatozoa, and the multiple potential applications these present, as well as the current limitations and leaps in our understanding and capabilities needed to overcome them. Further development and use of these technologies could lead to the identification of compounds which positively or negatively affect sperm cell motility or function, or novel platforms for toxicology or environmental chemical testing among other applications. Ultimately, each of these potential applications is also likely to increase understanding within the field of sperm biology.

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