Area and Perimeter: Melon Farm

Author(s):  
Rina Zazkis ◽  
Nathalie Sinclair ◽  
Peter Liljedahl
Keyword(s):  
Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1202
Author(s):  
Bojjibabu Chidipi ◽  
Syed Islamuddin Shah ◽  
Michelle Reiser ◽  
Manasa Kanithi ◽  
Amanda Garces ◽  
...  

In the heart, mitochondrial homeostasis is critical for sustaining normal function and optimal responses to metabolic and environmental stressors. Mitochondrial fusion and fission are thought to be necessary for maintaining a robust population of mitochondria, and disruptions in mitochondrial fission and/or fusion can lead to cellular dysfunction. The dynamin-related protein (DRP1) is an important mediator of mitochondrial fission. In this study, we investigated the direct effects of the micronutrient retinoid all-trans retinoic acid (ATRA) on the mitochondrial structure in vivo and in vitro using Western blot, confocal, and transmission electron microscopy, as well as mitochondrial network quantification using stochastic modeling. Our results showed that ATRA increases DRP1 protein levels, increases the localization of DRP1 to mitochondria in isolated mitochondrial preparations. Our results also suggested that ATRA remodels the mitochondrial ultrastructure where the mitochondrial area and perimeter were decreased and the circularity was increased. Microscopically, mitochondrial network remodeling is driven by an increased rate of fission over fusion events in ATRA, as suggested by our numerical modeling. In conclusion, ATRA results in a pharmacologically mediated increase in the DRP1 protein. It also results in the modulation of cardiac mitochondria by promoting fission events, altering the mitochondrial network, and modifying the ultrastructure of mitochondria in the heart.


2016 ◽  
Vol 693 ◽  
pp. 788-794
Author(s):  
Xiao Xiao Chen ◽  
Jun Zhao

The tool-workpiece contact zone is an important issue in the ball end milling process. This paper investigated the effects of tool inclination angles on the tool-workpiece contact zone, and variations of the cutting section area and perimeter with the increasing tilt and lead angles were also analyzed by geometrical modeling and measurement method for ball end milling process. The appropriate tool inclination angles, which could avoid the extrusion and friction between tool tip and the uncut materials, shorten the loading time on the cutting flute, and decrease the maximum cutting forces, could be preferentially selected according to the distribution characteristics of the tool-workpiece contact zone and the variations of the cutting section area and perimeter corresponding to various tool postures.


Palaios ◽  
2021 ◽  
Vol 36 (5) ◽  
pp. 165-172
Author(s):  
JAIME YESID SUÁREZ-IBARRA ◽  
CRISTIANE FRAGA FROZZA ◽  
SANDRO MONTICELLI PETRÓ ◽  
MARIA ALEJANDRA GÓMEZ PIVEL

ABSTRACT Planktonic foraminifera tests can suffer dissolution, which usually involves partial damage, weight loss, and fragmentation. Since planktonic foraminifera assemblages, consisting of different resistant/susceptible species, can be strongly modified by dissolution, it is imperative to quantify its effect. The fragmentation index proposed 50 years ago has been used widely to measure preservation of planktonic foraminifera tests, but calibrations to this method are necessary. Some revisions are based on assumptions, like a certain number of fragments produced by a unique test, which is then used to compare whole tests with the dissolution remains. Likewise, researchers do not agree on what they count and how they identify what they count. Here we present a standardized and less subjective method, called fragmentation intensity (FI), to better assess the fragmentation of planktonic foraminifera through image software analysis, which includes both fragmentation remains (fragments and broken tests) and their measured area and perimeter. When compared to calcium carbonate content, grain sand content, and planktonic foraminifera tests per gram of dry sediment, the FI method derived better correlation values than the broken and fragments indexes. Future studies, in varying oceanographic contexts, can test this method to improve confidence, and eventually possibly adapt the index into a proxy for calcium carbonate undersaturation.


Author(s):  
Ronnie Sabino Concepcion II ◽  
Jonnel Dorado Alejandrino ◽  
Sandy Cruz Lauguico ◽  
Rogelio Ruzcko Tobias ◽  
Edwin Sybingco ◽  
...  

Identifying the plant's developmental growth stages from seed leaf is crucial to understand plant science and cultivation management deeply. An efficient vision-based system for plant growth monitoring entails optimum segmentation and classification algorithms. This study presents coupled color-based superpixels and multifold watershed transformation in segmenting lettuce plant from complicated background taken from smart farm aquaponic system, and machine learning models used to classify lettuce plant growth as vegetative, head development and for harvest based on phytomorphological profile. Morphological computations were employed by feature extraction of the number of leaves, biomass area and perimeter, convex area, convex hull area and perimeter, major and minor axis lengths of the major axis length the dominant leaf, and length of plant skeleton. Phytomorphological variations of biomass compactness, convexity, solidity, plant skeleton, and perimeter ratio were included as inputs of the classification network. The extracted Lab color space information from the training image set undergoes superpixels overlaying with 1,000 superpixel regions employing K-means clustering on each pixel class. Six-level watershed transformation with distance transformation and minima imposition was employed to segment the lettuce plant from other pixel objects. The accuracy of correctly classifying the vegetative, head development, and harvest growth stages are 88.89%, 86.67%, and 79.63%, respectively. The experiment shows that the test accuracy rates of machine learning models were recorded as 60% for LDA, 85% for ANN, and 88.33% for QSVM. Comparative analysis showed that QSVM bested the performance of optimized LDA and ANN in classifying lettuce growth stages. This research developed a seamless model in segmenting vegetation pixels, and predicting lettuce growth stage is essential for plant computational phenotyping and agricultural practice optimization.


2015 ◽  
Vol 76 (1) ◽  
Author(s):  
Nurul Fathiah Mohamed Rosli ◽  
Muhammad Azmi Ayub ◽  
Roseleena Jaafan

The main objective of this research work is to anal yze the characteristics of a newly developed optical tactile sensor for sensing surface hardness. Many optical tactile sensors are bulky in size and lack of dexterity for biomedical applications. Therefore, this tactile sensor is design relative small in size and flexible for easier insertion in endoscopic surgery application. The characteristics of the tactile sensor are calibrated with respect to changes in the diameter, area and perimeter of a silicon tactile sensor subjected to normal forces applied at the point of interaction. A surface exploration computer algorithm to obtain the sensing information was developed to analyse the characteristic of the optical tactile sensor. The overall image anal ysis technique involves the following main stages: image acquisition (capturing of images), processing (thresholding, noise filtering and boundary detection ) and evaluation (force measurement). The measured forces were then compared to the actual forces to determine the accuracy of the tactile sensor’s characteristics. The results showed tluit the sensing characteristic with respect to changes in perimeter of the tactile sensor is more accurate compared to the other sensing characteristics. The outcomes of this research shows that the functionality of the developed new image anal ysis computer algorithm coupled with the silicone tactile sensor is suitable for biomedical applications such as in endoscopic surgery for measurement of tissue softness.


2021 ◽  
Vol 15 ◽  
Author(s):  
Vyshnavi Rallapalle ◽  
Annesha C. King ◽  
Michelle Gray

Huntington’s disease (HD) is a dominantly inherited, adult-onset neurodegenerative disease characterized by motor, psychiatric, and cognitive abnormalities. Neurodegeneration is prominently observed in the striatum where GABAergic medium spiny neurons (MSN) are the most affected neuronal population. Interestingly, recent reports of pathological changes in HD patient striatal tissue have identified a significant reduction in the number of parvalbumin-expressing interneurons which becomes more robust in tissues of higher disease grade. Analysis of other interneuron populations, including somatostatin, calretinin, and cholinergic, did not reveal significant neurodegeneration. Electrophysiological experiments in BACHD mice have identified significant changes in the properties of parvalbumin and somatostatin expressing interneurons in the striatum. Furthermore, their interactions with MSNs are altered as the mHTT expressing mouse models age with increased input onto MSNs from striatal somatostatin and parvalbumin-expressing neurons. In order to determine whether BACHD mice recapitulate the alterations in striatal interneuron number as observed in HD patients, we analyzed the number of striatal parvalbumin, somatostatin, calretinin, and choline acetyltransferase positive cells in symptomatic 12–14 month-old mice by immunofluorescent labeling. We observed a significant decrease in the number of parvalbumin-expressing interneurons as well as a decrease in the area and perimeter of these cells. No significant changes were observed for somatostatin, calretinin, or cholinergic interneuron numbers while a significant decrease was observed for the area of cholinergic interneurons. Thus, the BACHD mice recapitulate the degenerative phenotype observed in the parvalbumin interneurons in HD patient striata without affecting the number of other interneuron populations in the striatum.


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