Incorporation of Inorganic Phosphate into the Phospholipids of Isolated Chloroplasts

1989 ◽  
pp. 259-260
Author(s):  
P. G. Roughan ◽  
J. E. Cronan
Keyword(s):  
Inorganic Phosphate ◽  
Isolated Chloroplasts

ENZYMIC ACTIVITIES OF SUBCELLULAR PARTICLES FROM LEAVES: IV. PHOTOSYNTHETIC PHOSPHORYLATION AND PHOTOSYNTHESIS BY ISOLATED CHLOROPLASTS FROM PEA LEAVES

1959 ◽  
Vol 37 (6) ◽  
pp. 1217-1225 ◽  
Author(s):  
R. M. Smillie ◽  
G. Krotkov
Keyword(s):  
Carbon Dioxide ◽  
Inorganic Phosphate ◽  
Fixed Carbon ◽  
Spinach Chloroplasts ◽  
Green Pea ◽  
Isolated Chloroplasts

Chloroplasts were isolated in 0.35 M NaCl from green pea leaves. Such preparations formed ATP photosyathetically from AMP or ADP and inorganic phosphate. The conditions and cofactors of this reaction were studied. The rates of photosynthetic phosphorylation by isolated pea chloroplasts were compared with photosynthetic phosphorylation by spinach chloroplasts and with photosynthesis by intact pea leaves. The isolated pea chloroplasts also photosynthetically fixed carbon dioxide. The possible roles of chloroplasts and mitochondria in cellular phosphorylations are discussed.

Die Stöchiometrie der Photosynthese in isolierten Chloroplasten

1964 ◽  
Vol 19 (6) ◽  
pp. 470-480 ◽  
Author(s):  
Günter Jacobi
Keyword(s):  
Gas Phase ◽  
Electron Donor ◽  
Inorganic Phosphate ◽  
Oxygen Exchange ◽  
Light Reaction ◽  
Aerobic Conditions ◽  
Atp Formation ◽  
Long Term Experiments ◽  
Isolated Chloroplasts

As reported previously there is no stoichiometry between the amounts of ATP and NADPH2 formed in light by isolated chloroplasts of spinach. The amount of esterified inorganic phosphate is always too low. However, addition of ascorbate leads to an increase of ATP- but not of NADPH2-formation, independent of the gas phase present. Further experiments indicated that for the formation of “extra”-ATP with limiting amounts of NADP in long term experiments aerobic conditions are required. This additional ATP-formation occurs only in presence of ascorbate and starts when all NADP has been photoreduced. Ascorbate alone leads to photophosphorylation different from those systems, in which ascorbate is used as electron donor. The ascorbate induced photophosphorylation is strongly inhibited by o-phenanthroline and partly by cyanide. It is assumed that this ATP-formation is coupled with an oxygen exchange via a redox compound such as indophenol at the level of the second light reaction.

Plasmalemma localisation of inorganic phosphate in B cells pancreas

1978 ◽  
Vol 36 (2) ◽  
pp. 528-529
Author(s):  
F. B. P. Wooding ◽  
K. Pedley ◽  
N. Freinkel ◽  
R. M. C. Dawson
Keyword(s):  
Electron Microscope ◽  
B Cells ◽  
B Cell ◽  
Pancreatic Islets ◽  
High Glucose ◽  
Lead Acetate ◽  
Inorganic Phosphate ◽  
Slight Modification ◽  
Uranyl Acetate ◽  
Lead Phosphate

Freinkel et al (1974) demonstrated that isolated perifused rat pancreatic islets reproduceably release up to 50% of their total inorganic phosphate when the concentration of glucose in the perifusion medium is raised.Using a slight modification of the Libanati and Tandler (1969) method for localising inorganic phosphate by fixation-precipitation with glutaraldehyde-lead acetate we can demonstrate there is a significant deposition of lead phosphate (identified by energy dispersive electron microscope microanalysis) at or on the plasmalemma of the B cell of the islets (Fig 1, 3). Islets after incubation in high glucose show very little precipitate at this or any other site (Fig 2). At higher magnification the precipitate seems to be intracellular (Fig 4) but since any use of osmium or uranyl acetate to increase membrane contrast removes the precipitate of lead phosphate it has not been possible to verify this as yet.

Relation between intravascular electrolyte level and course of parturition in dairy cows

2013 ◽  
Vol 41 (05) ◽  
pp. 289-296 ◽  
Author(s):  
K. Seyrek-Intas ◽  
K. Failing ◽  
G. Yilmazbas Mecitoglu ◽  
H. Bostedt ◽  
D. Seyrek-Intas
Keyword(s):  
Dairy Cows ◽  
Inorganic Phosphate ◽  
Post Partum ◽  
Total Calcium ◽  
Normal Delivery ◽  
P Values ◽  
Normal Birth ◽  
Factors Influencing ◽  
Electrolyte Homeostasis ◽  
Convalescence Phase

Summary Objective: To determine the intravascular electrolyte status in dairy cows with respect to age and different courses of parturition to clarify etiological factors influencing peri- or intrapartal imbalances of electrolyte homeostasis. Material and methods: A total of 64 cows at birth were evaluated (primiparous n = 34, pluriparous n = 30). Thirty-three cows showed normal delivery, while 31 cows had a complicated birth. Blood samples were collected intra partum (i. p.) and 2, 4, 6, 8, 12, 16, 24 h post partum (p. p.) as well as [2, 3, 4, 5, 7, 10 days p. p. and levels of total calcium (Catotal), ionized calcium (Caion), inorganic phosphate (Pa), Mg, Na, K, Cl were determined. Results: The results revealed that electrolytes show great fluctuation during and immediately p. p. in relation to age. Already during parturition pluriparous cows had a lower Catotal and Pa concentration compared to primiparous animals. Cows with dystocia exhibited a more intensive and longer lasting decrease of Ca compared to cows with normal birth. In relation to age and severity of birth Pa concentration showed a differing but basically typical course for this electrolyte. Mg, Na, K and Cl concentrations were higher during and immediately after birth compared to p. p. values. Until day 10 p. p. these electrolyte concentrations declined more in older cows with dystocia compared to younger animals. However, the influence of dystocia on concentration of these electrolytes was milder in contrast to Ca and Pa. Conclusions: In summary, primarily older cows are predisposed to imbalances of electrolyte homeostasis intra partum and at the beginning of the lactation. These changes are potentiated in case of complications during parturition. Intravascular Catotal, Caion as well as Pa are most severely affected. Clinical relevance: These results may constitute the basis for a comprehensive metaphylaxis during the peripartal period, especially in cows after dystocia, to positively influence the early convalescence phase.

Biomimetic Extracellular Vesicles Embedded with Black Phosphorus for Molecular Recognition-Guided Biomineralization

2018 ◽  
Author(s):  
Yingqian Wang ◽  
Xiaoxia Hu ◽  
Lingling Zhang ◽  
Chunli Zhu ◽  
Jie Wang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Extracellular Vesicles ◽  
Inorganic Phosphate ◽  
Physiological Activity ◽  
Medical Engineering ◽  
Matrix Vesicles ◽  
Black Phosphorus ◽  
Photothermal Effect ◽  
Biological Processes ◽  
Extracellular Environment

Extracellular vesicles (EVs) are involved in the regulation of cell physiological activity and the reconstruction of extracellular environment. Matrix vesicles (MVs) are a type of EVs, and they participate in the regulation of cell mineralization. Herein, bioinspired MVs embedded with black phosphorus are functionalized with cell-specific aptamer (denoted as Apt-bioinspired MVs) for stimulating biomineralization. The aptamer can direct bioinspired MVs to targeted cells, and the increasing concentration of inorganic phosphate originated from the black phosphorus can facilitate cell biomineralization. The photothermal effect of the Apt-bioinspired MVs also positively affects mineralization. In addition, the Apt-bioinspired MVs display outstanding bone regeneration performance. Considering the excellent behavior of the Apt-bioinspired MVs for promoting biomineralization, our strategy provides a way of designing bionic tools for studying the mechanisms of biological processes and advancing the development of medical engineering.<br>

Correlative Study of Bone-Related Biochemicals and Endocrine Changes in Perimenopausal Women in Enugu, Nigeria.

2020 ◽  
Vol 23 ◽  
Author(s):  
Chinwe Ifeoma Ikegwuonu ◽  
Ikenna Kingsley Uchendu ◽  
Chukwudi Ignatius Maduka
Keyword(s):  
Inorganic Phosphate ◽  
Premenopausal Women ◽  
Positive Association ◽  
Biochemical Changes ◽  
Health Conditions ◽  
Hormonal Changes ◽  
Negatively Associated ◽  
Perimenopausal Women ◽  
Endocrine Parameters ◽  
Apparently Healthy

Background and Objective: Perimenopause is a physiological occurrence in women, and is characterized by endocrine and biochemical changes. During perimenopause phase, many derangements or abnormal health conditions start developing as a result of hormonal changes. These derangements in health conditions and biochemical changes lead to higher incidence of metabolic syndrome (MetS) occurrence with or without bone involvement. There is scarcity of information on MetS in Enugu, Southern Nigeria and there is no available data on the correlation of selected bone-related biochemicals with endocrine parameters and MetS in perimenopausal women from the region. Material and Methods: We consecutively sampled 200 apparently healthy women, and categorized them into 120 perimenopausal women (age ( Results: Calcium was predominantly high in the three criteria (p<0.05). LH and FSH showed positive correlation with FPG while E2 was negatively associated with FPG. Similarly LH showed positive association with inorganic phosphate while E2 was negatively associated with alkaline phosphatase (p<0.05). Conclusion: Perimenopausal women are at higher risk for developing osteoporosis than premenopausal women. This emphasizes the need for timely diagnosis of osteoporosis in perimenopausal women.

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