Glycosaminoglycan Blotting and Detection After Electrophoresis Separation

2015 ◽  
pp. 119-127 ◽  
Author(s):  
Nicola Volpi ◽  
Francesca Maccari
Keyword(s):  
Electrophoresis Separation

Capillary Electrophoresis Separation of Artepillin C: Determination in Brazilian Green Propolis

2021 ◽  
Author(s):  
Fabio Galeotti ◽  
Federica Capitani ◽  
Francesca Maccari ◽  
Veronica Mantovani ◽  
Nicola Volpi
Keyword(s):  
Capillary Electrophoresis ◽  
Voltage Gradient ◽  
Food Ingredients ◽  
Therapeutic Applications ◽  
Optimal Separation ◽  
Artepillin C ◽  
Brazilian Propolis ◽  
Main Component ◽  
Electrophoresis Separation ◽  
Brazilian Green Propolis

Abstract Propolis is important in complementary and alternative medicine having well-known therapeutic applications. Artepillin C, a main component of Brazilian (green) propolis, has attracted great attention for its anticancer action. Consequently, the synthesis of artepillin C has been reported but, due to the limited yield and elevated costs, this biomolecule is largely produced from Brazilian propolis. We report the capillary electrophoresis (CE) separation of artepillin C in Brazilian propolis also comparing the results with those of HPLC-UV-MS. Optimal separation was obtained with a simple buffer constituted of sodium tetraborate 30 mM pH 9.2 and detection at 210 nm. Artepillin C and the polyphenols of propolis were fully separated with a voltage gradient of 30 to 8 kV and a current of 300 μA for a total run of 50 min. The sensitivity of CE-UV was 22 times greater than HPLC-UV and 100 times more than HPLC-MS with also a stronger reduction in the run time and a greater robustness and reproducibility. The development of CE as an effective and reliable method for the analysis of artepillin C is desired as the standardized quality controls are essential before propolis or its biomolecules can be adopted routinely in nutraceuticals, food ingredients and therapeutic applications.

Capillary electrophoresis separation of human milk neutral and acidic oligosaccharides derivatized with 2-aminoacridone

2014 ◽  
Vol 35 (6) ◽  
pp. 811-818 ◽  
Author(s):  
Fabio Galeotti ◽  
Giovanni V. Coppa ◽  
Lucia Zampini ◽  
Francesca Maccari ◽  
Tiziana Galeazzi ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Human Milk ◽  
Capillary Electrophoresis Separation ◽  
Electrophoresis Separation

Capillary zone electrophoresis separation of azaarenes with sensitive UV absorption photometric detection after cationic solid phase extraction and field amplified sample stacking

Fuel ◽  
2014 ◽  
Vol 129 ◽  
pp. 20-26 ◽  
Author(s):  
Elaine Rocha da Luz ◽  
Cabrini Ferraz de Souza ◽  
Anastácia Sá ◽  
Alessandra Licursi M.C. da Cunha ◽  
Caroline F.J. Franco ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Capillary Zone Electrophoresis ◽  
Solid Phase ◽  
Uv Absorption ◽  
Phase Extraction ◽  
Sample Stacking ◽  
Photometric Detection ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Electrophoresis Separation
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