Effect of Commercially Available Synthetic Insulin on the Biofilm Formation in S. aureus and E. coli Bacterial Strains

2019 ◽  
pp. 755-759
Author(s):  
Jasmin Novalić ◽  
Berina Bektić
Keyword(s):  
Biofilm Formation ◽  
Bacterial Strains ◽  
E Coli

scholarly journals Synthesis, Molecular Docking, and Biofilm Formation Inhibitory Activity of Bis(Indolyl)Pyridines Analogues of the Marine Alkaloid Nortopsentin

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4112
Author(s):  
Heba M. Abo-Salem ◽  
Hayam A. Abd El Salam ◽  
Anhar M. Abdel-Aziem ◽  
Mohamed S. Abdel-Aziz ◽  
Eslam Reda El-Sawy
Keyword(s):  
Biofilm Formation ◽  
Ammonium Acetate ◽  
Binding Energies ◽  
Bacterial Strains ◽  
Topoisomerase Iv ◽  
Gram Positive ◽  
One Pot ◽  
Gram Negative ◽  
E Coli ◽  
Marine Alkaloid

An efficient and simple protocol for the synthesis of a new class of diverse bis(indolyl)pyridines analogues of the marine alkaloid nortopsentin has been reported. A one-pot four-component condensation of 3-cyanocarbomethylindole, various aldehyde, 3-acetylindole, and ammonium acetate in glacial acetic acid led to the formation of 2,6-bis(1H-indol-3-yl)-4-(substituted-phenyl)pyridine-5-carbonitriles. Additionally, 2,6-bis(1H-indol-3-yl)-4-(benzofuran) pyridine-5-carbonitriles were prepared via a one-pot four-component condensation of 3-cyanocarbomethylindole, various N-substituted-indole-3-aldehydes, 2-acetylbenzofuran, and ammonium acetate. The synthesized compounds were evaluated for their ability to inhibit biofilm formation against the Gram-positive bacterial reference strains Staphylococcus aureus ATCC 6538 and the Gram-negative strain Escherichia coli ATCC 25922. Some of the new compounds showed a marked selectivity against the Gram-positive and Gram-negative strains. Remarkably, five compounds 4b, 7a, 7c, 7d and 8e demonstrated good antibiofilm formation against S. aureus and E. coli. On the other hand, the release of reducing sugars and proteins from the treated bacterial strains over the untreated strains was considered to explain the disruption effect of the selected compound on the contact cells of S. aureus and E. coli. Out of all studied compounds, the binding energies and binding mode of bis-indole derivatives 7c and 7d were theoretically the best thymidylate kinase, DNA gyrase B and DNA topoisomerase IV subunit B inhibitors.

scholarly journals Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study

2021 ◽  
Vol 10 (5) ◽  
pp. e15310514701
Author(s):  
Gisele Alborghetti Nai ◽  
Denis Aloísio Lopes Medina ◽  
Cesar Alberto Talavera Martelli ◽  
Mayla Silva Cayres de Oliveira ◽  
Isadora Delfino Caldeira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Chronic Wounds ◽  
In Vitro Study ◽  
Multiple Infections ◽  
Bacterial Strains ◽  
E Coli ◽  
Colonization Density ◽  
Silicone Prostheses

Staphylococcus aureus biofilms have been recognized as a leading cause of multiple infections, including implant-associated infections and chronic wounds. We evaluated the colonization capacity of two distinct textured prostheses by different bacterial strains. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis and Enterococcus faecalis were evaluated. Initially, the hydrophobicity and biofilm formation capacity were determined. Subsequently, 20 fragments of vascular prosthesis and 20 silicone prostheses were embedded in suspensions with the microorganisms and incubated. The prostheses were then sown in culture medium and incubated for 48 hours. Petri dishes were photographed and analyzed by fractal dimension. The Kruskal-Wallis test and the Dunn test were applied for the analysis of biofilm formation. To compare the mean intensity for the type of bacteria and the type of prosthesis, a general linear model was applied. Staphylococcus aureus was the bacterium with the highest colonization density in both prostheses (p = 0.0001). E. coli showed strong adherence in the biofilm formation capacity test (p = 0.0001), however, it did not colonize either prosthesis. We demonstrated that Staphylococcus aureus has a greater affinity for vascular and silicone prostheses than other bacteria.

scholarly journals When Beneficial Biofilm on Materials Is Needed: Electrostatic Attachment of Living Bacterial Cells Induces Biofilm Formation

2021 ◽  
Vol 8 ◽  
Author(s):  
Dmitrii Deev ◽  
Iaroslav Rybkin ◽  
Tomaž Rijavec ◽  
Aleš Lapanje
Keyword(s):  
Biofilm Formation ◽  
Bacterial Attachment ◽  
Wall Structure ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Microbial Composition ◽  
E Coli ◽  
Environmental Bacteria ◽  
Artificial Biofilm ◽  
Biofilm Prevention

Bacterial attachment is crucial in many biotechnological applications, but many important bacterial strains cannot form biofilms. Biofilms can damage materials, and current strategies to manage biofilms are focused on inhibition and removal of biofilm. Biofilm formation is inevitable when materials are exposed to microbes and instead of biofilm prevention, we propose management of microbial composition by formation of biofilms with beneficial microbes. Since bacteria need to overcome a high repulsive force to attach to the surface and later to grow and multiply on it, electrostatic modification of the surfaces of cells or the material by polyelectrolytes (PE) was used in our approach, enabling efficient attachment of viable bacterial cells. Since highly positively charged PEs are known to be bactericidal, they were acetylated to reduce their toxicity, while preserving their net positive charge and ensuring cell viability. In our study bacterial strains were selected according to their intrinsic capability of biofilm formation, their shape variety and cell wall structure. These strains were tested to compare how the artificially prepared vs. natural biofilms can be used to populate the surface with beneficial bacteria. Using an artificial biofilm constructed of the potentially probiotic isolate Bacillus sp. strain 25.2. M, reduced the attachment and induced complete inhibition of E. coli growth over the biofilm. This study also revealed that the modification of the surfaces of cells or material by polyelectrolytes allows the deposition of bacterial cells, biofilm formation and attachment of biofilm non-forming cells onto surfaces. In this way, artificial biofilms with extended stability can be constructed, leading to selective pressure on further colonization of environmental bacteria.

scholarly journals The relationship between biofilm formation, genes of virulence and iron metabolism in Escherichia coli

2018 ◽  
Author(s):  
Lívia Handrová ◽  
Anna Čuvalová ◽  
Vladimír Kmeť
Keyword(s):  
Escherichia Coli ◽  
Iron Metabolism ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Opportunistic Infections ◽  
Bacterial Species ◽  
Pcr Amplification ◽  
Bacterial Strains ◽  
E Coli ◽  
Wide Range

Escherichia coli is known as one of the bacterial species with the widest adaptability to variety of niches either within organisms or outside in environment. Most strains of E. coli are of low virulence and associated with opportunistic infections, whereas others are highly virulent. The success of E. coli in colonising such a wide range of hosts and environments is basically due to a noticeable ductility in exploiting the available resources. It is becoming increasingly clear that biofilms have an enormous impact on medicine because since 65% of animal and human bacterial infections involve biofilms. In present study, we isolated strains of E. coli from animals. 19 interesting isolates were selected and tested by PCR amplification to virulence – iutA, cvaC, iss, tsh, papC, kps, iha and iron metabolism genes – sitA, feoB, irp2, fyuA, iroN, ireA. The ability of biofilm formation was assessed in a quantitative assay using a microtiter-plate test. Bacterial strains were grown on BHI. We divided isolates of E. coli into four classes: very weak (63.0%), weak (10.5%), moderate (10.5%) and strong (16.0%) biofilm producers. Representation genes of virulence were highly in isolates from very weak biofilm producers – from 7 genes were 6 highly; only papC (P fimbrial adhesin) was low. Genes of iron metabolism were different. Genes – sitA, fyuA, ireA in strong isolates producing biofilm and feoB, irp2, iroN in weak producers were most represented. The results show possible relation between presence virulence factor and low biofilm formation.

Bioceramic Materials Show Reduced Pathological Biofilm Formation

2014 ◽  
Vol 631 ◽  
pp. 448-453 ◽  
Author(s):  
Corrado Piconi ◽  
Andrei Cristian Ionescu ◽  
Andrea Cochis ◽  
Erica Iasi ◽  
Eugenio Brambilla ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Morphological Data ◽  
Alumina Matrix ◽  
Bacterial Strains ◽  
E Coli ◽  
Viable Biomass ◽  
Alumina Matrix Composite ◽  
Scanning Electron

The aim of the present work was to assess the surface ability of three bioceramic materials (A: alumina BIOLOX®forte; B: Si3N4; C: alumina matrix composite BIOLOX®delta) to inhibit bacterial biofilm formation. For this purpose, ceramic disks at standardized roughness (Ra = 0,25 μm) were used as test materials while commercial polystyrene was considered as control. Two biofilm-producing bacterial strains (S. epidermidis ATCC14990, Escherichia coli ATCC25922) were used for experiments. The viable biomass was assessed by the metabolic MTT assay after 24h incubation. Morphological data regarding biofilms structure were obtained by scanning electron microscopy. In general, results revealed that all bioceramics materials were significantly less colonized compared to polystyrene. The degree of biofilm formation onto bioceramics ranged between about 30 to 60% less than the polystyrene control. Moreover, some differences were noticed by comparing the three bioceramics inhibition ratio: bioceramic A showed significanlty less S. epidermidis biofilm formation (p<0.005) compared to B and C that showed similar performance. Conversely, no difference were noted for E. coli biofilm amount for A, B and C. In conclusion, the tested materials showed capability to reduce biofilm formation to a different extent depending on the tested bacterial strains.

scholarly journals Tryptophan Silver Nanoparticles Synthesized by Photoreduction Method: Characterization and Determination of Bactericidal and Anti-Biofilm Activities on Resistant and Susceptible Bacteria

2019 ◽  
Vol 12 ◽  
pp. 117864691983167 ◽  
Author(s):  
Daniella dos Santos Courrol ◽  
Carla Regina Borges Lopes ◽  
Camila Bueno Pacheco Pereira ◽  
Marcia Regina Franzolin ◽  
Flávia Rodrigues de Oliveira Silva ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Irradiation Time ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Static Conditions

The high rates of antibiotics use in hospitals have resulted in a condition where multidrug-resistant pathogens have become a severe threat to the human health worldwide. Therefore, there is an increasing necessity to identify new antimicrobial agents that can inhibit the multidrug-resistant bacteria and biofilm formation. In this study, antibacterial and anti-biofilm activities of tryptophan silver nanoparticles (TrpAgNP) were investigated. The TrpAgNPs were synthesized by photoreduction method, and the influence of irradiation time and concentration of reagents were analyzed. The nanoparticles were characterized by transmission electron microscopy, Zeta Potential and (UV)-absorption spectra. The antibacterial activity of TrpAgNPs was tested for antibiotic-resistant and susceptible pathogens, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Salmonella typhimurium, and Pseudomonas aeruginosa, evaluating the influence of photoreduction parameters in bactericidal effect. The results have shown that TrpAgNPs solutions with lower tryptophan/silver nitrate (AgNO3) ratio and higher AgNO3 concentration have higher bactericidal action against bacteria with inhibition of ~100% in almost all studied bacterial strains. The antimicrobial activity of TrpAgNPs within biofilms generated under static conditions of antibiotic-resistant and susceptible strains of S. aureus, S. epidermidis, E. coli, K. pneumoniae, C. freundii, and P. aeruginosa was also investigated. The results showed that TrpAgNPs have an inhibitory effect against biofilm formation, exceeding 50% in the case of Gram-negative bacteria ( E. coli, K. pneumoniae, C. freundii, and P. aeruginosa—54.8% to 98.8%). For Gram-positive species, an inhibition of biofilm formation of 68.7% to 72.2 % was observed for S. aureus and 20.0% to 40.2% for S. epidermidis.

scholarly journals Isolation and Culture Dependent Characterization of Escherichia coli from the Sewage Waste Water of Lahore, Pakistan

2019 ◽  
pp. 1-13
Author(s):  
Abida Bano ◽  
Basharat Ali
Keyword(s):  
Waste Water ◽  
Drinking Water ◽  
Shiga Toxin ◽  
Biofilm Formation ◽  
Sewage Water ◽  
Cross Contamination ◽  
Bacterial Strains ◽  
E Coli ◽  
Toxin Genes ◽  
Shiga Toxin Genes

Aims: Infectious disease haemorrhagic uremic syndrome (HUS), present in sewage water, its cross contamination with drinking water may affect the community. This study was conducted to isolate the pathogenic strain of E. coli O157 from the sewage waste water of Lahore, Pakistan. The aim of this study was to evaluate biofilm formation and antibiotic susceptibility pattern for the isolated strains. Selective Hichrome EC O157 agar media was used for the isolation of E. coli. Methodology: Taxonomic status of strain was confirmed by 16S rRNA gene sequencing. Serotyping of E. coli O157 strains was accomplished by ProlexTM E. coli O157 Latex Test Reagent Kit results for O157. Bacterial strains were also evaluated for biofilm formation, toxin related genes (stx1, stx2, stx2c, stx2d) and antibiotic sensitivity. Results: Bacterial strains showed resistance against amoxicillin, tobramycin, tetracycline and nitrofurantoin antibiotics. Maximum biofilm formation was shown by strain E124 when used as monoculture. In cocultures, strains E35 and E101 were the most efficient biofilm formers. PCR amplification recorded negative results for shiga toxin genes. Conclusion: Shiga toxin genes were not present in any of the E. coli strain, from which we can suggest that our environment is free of shiga toxin genes. E. coli was present in sewage water, its cross contamination with drinking water may affect the community. Therefore, waste water should be treated properly before discarding it into the common water bodies.

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF 3-FORMYL INDOLE BASED SCHIFF BASES

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Singh Gurvinder ◽  
Singh Prabhsimran ◽  
Dhawan R. K.
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli

In order to develop new antimicrobial agents, a series of 3-formyl indole based Schiff bases were synthesized by reacting 3-formyl indole(indole-3-carboxaldehyde) with substituted aniline taking ethanol as solvent. The reaction was carried in the presence of small amount of p-toluene sulphonic acid as catalyst.All the synthesized compounds were characterized by IR, 1H-NMR spectral analysis. All the synthesized compounds were evaluated for antimicrobial activity against two gram positive bacterial strains (B. subtilisand S. aureus) and two gram negative bacterial strains (P. aeruginosaand E. coli) and one fungal strain (C. albicans). All the synthesized compounds were found to have moderate to good antimicrobial activity. The  standard drug amoxicillin, fluconazole were used for antimicrobial activity. Among the synthesized compounds, the maximum antimicrobial activity was shown by compounds GS04, GS07, GS08 and GS10.

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