Isolation and Identification of Campylobacter spp. in Poultry

2016 ◽  
pp. 19-35 ◽  
Author(s):  
Omar A. Oyarzabal ◽  
Heriberto Fernández
Keyword(s):  
Isolation And Identification ◽  
Campylobacter Spp

Isolation and Identification of Campylobacter spp. from Poultry and Poultry By-Products in Tunisia by Conventional Culture Method and Multiplex Real-Time PCR

2017 ◽  
Vol 80 (10) ◽  
pp. 1623-1627 ◽  
Author(s):  
Hela Jribi ◽  
Hanen Sellami ◽  
Siala Mariam ◽  
Salma Smaoui ◽  
Asma Ghorbel ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Poultry Meat ◽  
Culture Methods ◽  
Isolation And Identification ◽  
Conventional Culture ◽  
Multiplex Pcr Assay ◽  
Thermophilic Campylobacter ◽  
By Products ◽  
Campylobacter Spp

ABSTRACT Thermophilic Campylobacter spp. are one of the primary causes of bacterial human diarrhea. The consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. The aims of this study were to determine the prevalence of thermophilic Campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm Campylobacter jejuni and Campylobacter coli isolates by using the multiplex PCR assay. Two hundred fifty fresh poultry samples were collected from a variety of supermarkets and slaughterhouses located in Sfax, Tunisia, including chicken (n =149) and turkey (n =101). The samples were analyzed using conventional microbiological examinations according to the 2006 International Organization for Standardization method (ISO 10272-1) for Campylobacter spp. Concurrently, a real-time PCR was used for identification of C. jejuni and C. coli. Of the 250 samples of poultry meat and poultry by-products, 25.6% (n = 64) were contaminated with Campylobacter spp. The highest prevalence of Campylobacter spp. was found in chicken meat (26.8%) followed by turkey meat (23.7%). Among the different products, poultry breasts showed the highest contamination (36.6%) followed by poultry by-products (30%), poultry wings (28%) and poultry legs (26%) showed the lowest contamination, and no contamination was found on neck skin. Of the 64 thermophilic Campylobacter isolates, C. jejuni (59.7%) was the most frequently isolated species and 10.9% of the isolates were identified as C. coli. All of the 64 Campylobacter isolates identified by the conventional culture methods were further confirmed by PCR. The seasonal peak of Campylobacter spp. contamination was in the warm seasons (spring and summer). The study concluded that high proportions of poultry meat and poultry by-products marketed in Tunisia are contaminated by Campylobacter spp. Furthermore, to ensure food safety, poultry meats must be properly cooked before consuming.

scholarly journals Isolation, identification, and antimicrobial susceptibility pattern of Campylobacter jejuni and Campylobacter coli from cattle, goat, and chicken meats in Mekelle, Ethiopia

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246755
Author(s):  
Yohans Hagos ◽  
Getachew Gugsa ◽  
Nesibu Awol ◽  
Meselu Ahmed ◽  
Yisehak Tsegaye ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Campylobacter Coli ◽  
Susceptibility Pattern ◽  
Isolation And Identification ◽  
Raw Meat ◽  
Antibiotic Susceptibility Test ◽  
Meat Samples ◽  
Campylobacter Spp

Campylobacter jejuni and Campylobacter coli are globally recognized as a major cause of bacterial foodborne gastroenteritis. A cross-sectional study was conducted from October 2015 to May 2016 in Mekelle city to isolate, identify, and estimate the prevalence of C. jejuni and C. coli in raw meat samples and to determine their antibiotic susceptibility pattern. A total of 384 raw meat samples were randomly collected from bovine (n = 210), goat (n = 108), and chicken (n = 66), and isolation and identification of Campylobacter spp. were performed using standard bacteriological techniques and PCR. Antibiotic susceptibility test was performed using disc diffusion method. Of the total 384 raw meat samples, 64 (16.67%) were found positive for Campylobacter spp. The highest prevalence of Campylobacter spp. was found in chicken meat (43.93%) followed by bovine meat (11.90%) and goat meat (9.25%). The most prevalent Campylobacter spp. isolated from meat samples was C. jejuni (81.25%). The overall prevalence of Campylobacter in restaurants, butcher shops, and abattoir was 43.93%, 18.30%, and 9.30%, respectively. 96.8%, 81.25%, 75%, and 71% of the Campylobacter spp. isolates were sensitive to norfloxacin, erythromycin, chloramphenicol, and sulphamethoxazole-trimethoprim, respectively. However, 96.9%, 85.9%, and 50% of the isolates were resistant to ampicillin, amoxicillin, and streptomycin, respectively. Strains that developed multi-drug resistant were 68.7%. The result of this study revealed the occurrence of Campylobacter in bovine, goat, and chicken meats. Hence, there is a chance of acquiring infection via consumption of raw or undercooked meat. Thus, implementation of hygienic practices from a slaughterhouse to the retailers, proper handling and cooking of foods of meat are very important in preventing Campylobacter infection.

scholarly journals Investigating Carriage, Contamination, Antimicrobial Resistance and Assessment of Colonization Risk Factors of Campylobacter spp. in Broilers from Selected Farms in Thika, Kenya

2019 ◽  
pp. 1-18 ◽  
Author(s):  
Mwajuma K. Abubakar ◽  
Anne W. T. Muigai ◽  
Perpetual Ndung’u ◽  
Samuel Kariuki
Keyword(s):  
Antimicrobial Resistance ◽  
Study Design ◽  
Public Health Problem ◽  
Small Scale ◽  
Major Public Health Problem ◽  
Isolation And Identification ◽  
Broiler Meat ◽  
Cross Sectional ◽  
North East ◽  
Campylobacter Spp

Aims: To investigate carriage and contamination rates of chicken broiler meat, the factors that are associated with Campylobacter spp. colonization and its phenotypic and genotypic antimicrobial resistance from Thika small-scale poultry farms. Study Design: The study design was cross-sectional and laboratory based, it employed simple random sampling across 18 small-scale farms. Site and Duration of Study: The study was conducted between August and December 2017 at Thika sub-county, a town located 42 Km North East of Nairobi. Methodology: One hundred and eighty five cloaca swab samples from live broilers and 158 neck swab samples from broiler carcasses were collected. Isolates were obtained by plating method using mCCDA, conventional methods and duplex PCR were used for the isolation and identification of Campylobacter species. Results: Carriage prevalence was at 15.67%, significantly (P = .000) lower than contamination prevalence detected at 30.37%. While the overall Campylobacter spp. prevalence was 22.45%.  Risk of Campylobacter colonization in the flock doubled in feeding broilers with chicken waste and older poultry, at (OR: 2.57, 95% CI: 0.19 - 34.47) and (OR: 2.00, 95% CI: 0.312 - 12.84) respectively. The Campylobacter spp. were resistant (P < .05) against Ciprofloxacin, Streptomycin, and Trimethoprim between carriage and contamination. MDR was 79.22%; XDR was 12.98% while no PDR recorded. Conclusion: Broilers in Thika region are potentially important source of human infection and possible continuity of infection from the threat posed by Campylobacter carrier broilers. Presence of sulI and dhfr genes with high resistance observed for quinolones, sulfonamides, ß-lactams and trimethoprim, thus posing a major public health problem for consumers of poultry products.

scholarly journals Occurrence and antimicrobial resistance of Campylobacter jejuni isolates from poultry in Casablanca-Settat, Morocco

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Khadija Es-soucratti ◽  
Abderrahman Hammoumi ◽  
Brahim Bouchrif ◽  
Rajaa Asmai ◽  
Houda En-nassiri ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Public Health Problem ◽  
Poultry Production ◽  
Isolation And Identification ◽  
Drug Of Choice ◽  
Amoxicillin Clavulanic Acid ◽  
Commercial Poultry ◽  
The City ◽  
Campylobacter Spp

Campylobacteriosis and Campylobacter spp. resistance to antibiotics represents a serious worldwide public health problem thermophilic Campylobacters, in particular, are major causes of gastroenteritis in humans. The aim of this study was to determine the prevalence and antimicrobial resistance of Campylobacter jejuni isolated from chicken droppings, of commercial poultry in the city of Casablanca, Morocco. Between February and September 2017, 140 samples of chicken droppings were collected and analyzed by classical bacteriology methods for isolation and identification according to Moroccan Standard NM ISO/TS 10272-3 (2013), followed by molecular identification (PCR: polymerase chain reaction). Among the 140 samples, 102 (73%) were positive by Campylobacter spp. tests and 38 (27.14 %) were negative to Campylobacter spp. Among the positive colonies, 41 (40, 2%) were C. jejuni. Of the 41 C. jejuni isolates, resistance was detected to tetracycline (100%), erythromycin (97%), ampicillin (85%), ciprofloxacin (77%), amoxicillin/ clavulanic acid (61.4%), and gentamicin (12.0%). In conclusion, the data obtained in the current study demonstrate that the majority of C. jejuni isolates evaluated were resistant to antimicrobials of the cycline, macrolide, and fluoroquinolone families, and all of the isolates were susceptible to gentamicin. Fluoroquinolone is the drug of choice for treating Campylobacter infections. These results underline the need for prudent use of antibiotics in poultry production to minimize the spread of antibioticresistant Campylobacter spp.

Isolation and Identification of Enteropathogenic Campylobacter spp. from Chicken Samples in Taipei

2000 ◽  
Vol 63 (3) ◽  
pp. 304-308 ◽  
Author(s):  
DANIEL YANG-CHIH SHIH
Keyword(s):  
Selective Medium ◽  
Species Level ◽  
Retail Market ◽  
Isolation And Identification ◽  
Retail Markets ◽  
Selective Media ◽  
Genus Level ◽  
Identification Method ◽  
Test Kit ◽  
Campylobacter Spp

A total of 95 chicken samples that consisted of 34 whole chickens, 32 organs (gizzards and livers), and 29 chicken parts (drumsticks, wings, and breasts), collected from traditional retail markets (no chilling facilities) and supermarkets in Taipei, were examined for the occurrence of enteropathogenic campylobacters. Three selective media, Peterz's charcoal cefoperazone deoxycholate agar, Campy-Cefex agar, and charcoal-based selective medium, were evaluated for their efficacy to isolate Campylobacter spp. from chicken samples. The results showed that there were no differences among the three media to isolate Campylobacter spp. from all chicken samples (P &gt; 0.05). However, there were markedly different isolation rates of campylobacters between supermarket and retail market (P &lt; 0.05). Enteropathogenic campylobacters (C. jejuni and C. coli) were found on 68% of whole chickens, 100% of chicken parts, and 100% of organs from retail markets. In supermarkets, the isolation rates of these campylobacters from whole chickens, chicken parts, and organs were 42%, 53%, and 60%, respectively. The low isolation rates of the two campylobacters isolated from chicken samples in supermarkets differed statistically from those obtained from traditional retail markets (P &lt; 0.10). The API CAMPY test kit also was evaluated for the identification of Campylobacter spp. as compared with the conventional identification method. The results showed that the API CAMPY test kit (Biomerieux, Marcy-l'Etoile, France) could efficiently detect 87 Campylobacter spp. isolates from chicken samples examined, with 100% agreement at the genus level to 94% at the species level as compared with conventional methods.

scholarly journals Antimicrobial Susceptibility of Campylobacter isolates in the Capital of North Macedonia

PRILOZI ◽  
2019 ◽  
Vol 40 (2) ◽  
pp. 73-80
Author(s):  
Elena Trajkovska-Dokic ◽  
Kiril Mihajlov ◽  
Gordana Mirchevska ◽  
Marko Kostovski ◽  
Aneta Blazevska ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Antimicrobial Treatment ◽  
Rational Approach ◽  
Beta Lactams ◽  
Isolation Rate ◽  
Isolation And Identification ◽  
Microbiological Methods ◽  
Significant Difference ◽  
Acute Enteritis ◽  
Campylobacter Spp

Abstract Background: Campylobacter infections are typically self-limited, but in cases with severe enteritis, immuno-compromised system and bacteremia, an appropriate antimicrobial treatment is demanding. Our study aim was to determine the isolation rate of Campylobacter among patients with acute enteritis in the capital of North Macedonia and its antimicrobial susceptibility. Material and methods: A total number of 3820 patients clinically diagnosed as acute enteritis, were included in the study. Stool samples were collected and Campylobacter was isolated and identified by classical microbiological methods. Antimicrobial susceptibility of all isolates to Ceftriaxone, Amoxicillin-clavulonic acid, Erythromycin, Ciprofloxacin, Tetracycline and Gentamicin was determined by disc-diffusion technique. Additionally, minimal inhibitory concentrations of all Campylobacter isolates against erythromycin, ciprofloxacin and tetracycline were determined by Epsilon gradient tests. Results: Campylobacter species was isolated in 97 patients. Although the mean isolation rate of Campylobacter spp. during the whole study period was 2.53%, a statistically significant increase was detected in 2016 and 2017, in comparison with the data from previous four years of the study. The isolation rate of Campylobacter spp. didn’t reveal statistically significant difference between males and females (p > 0.05). 46.4 % of patients with Campylobacter enteritis were children at the age under 15 years. Forty-three C. jejuni isolates were susceptible to all six antibiotics, but the remaining 44 isolates revealed resistance to at least one antibiotic. C. coli isolates were resistant to 3 antibiotics simultaneously. Two C. coli isolates only, were susceptible to all 6 antibiotics. 40.90% of C. jejuni and 50% of C. coli isolates were resistant to beta-lactams, fluoroquinolones and tetracyclines, simultaneously. Conclusion: The increase of the isolation rate of Campylobacter from patients with acute enteritis indicates the need for permanent isolation and identification of Campylobacter from every clinically diagnosed patient, as acute enteritis. Erythromicin is the most effective antibiotic for treatment of Campylobacter enteritis in our patients. The high level of Campylobacter resistance to beta-lactams, fluoroquinolones and tetracyclines requires more rational approach in the treatment of Campylobacter enteritis

Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang
Keyword(s):  
Chemical Constituents ◽  
Isolation And Identification

Schätzung der Krankheitslast durch Campylobacter spp. für das Jahr 2014 in Deutschland

2017 ◽  
Author(s):  
J Lackner ◽  
C Müller-Graf ◽  
M Greiner
Keyword(s):  
Campylobacter Spp
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