A Microcosm Study on the Impact of Acidification on Feeding, Survival, Nauplii Production Rate, Post-embryonic Development and Nutritional Composition of Marine Copepod

Author(s):  
T. Jayalakshmi ◽  
P. Santhanam
2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Jiang-Yu Ye ◽  
Yue Pan ◽  
Yong Wang ◽  
Yi-Chao Wang

Abstract Purpose This study utilized the principle that the bacteriorhodopsin (BR) produced by Halobacterium salinarum could increase the hydrogen production of Rhodobacter sphaeroides. H. salinarum are co-cultured with R. sphaeroides to determine the impact of purple membrane fragments (PM) on R. sphaeroides and improve its hydrogen production capacity. Methods In this study, low-salinity in 14 % NaCl domesticates H salinarum. Then, 0–160 nmol of different concentration gradient groups of bacteriorhodopsin (BR) and R. sphaeroides was co-cultivated, and the hydrogen production and pH are measured; then, R. sphaeroides and immobilized BR of different concentrations are used to produce hydrogen to detect the amount of hydrogen. Two-chamber microbial hydrogen production system with proton exchange membrane-assisted proton flow was established, and the system was operated. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. Results H salinarum can still grow well after low salt in 14% NaCl domestication. When the BR concentration is 80 nmol, the highest hydrogen production reached 217 mL per hour. Both immobilized PC (packed cells) and immobilized PM (purple membrane) of H. salinarum could promote hydrogen production of R. sphaeroides to some extent. The highest production of hydrogen was obtained by the coupled system with 40 nmol BR of immobilized PC, which increased from 127 to 232 mL, and the maximum H2 production rate was 18.2 mL−1 h−1 L culture. In the 192 h experiment time, when the potential is 0.3 V, the hydrogen production amount can reach 920 mL, which is 50.3% higher than the control group. Conclusions The stability of the system greatly improved after PC was immobilized, and the time for hydrogen production of R. sphaeroides significantly extended on same condition. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. These results are helpful to build a hydrogen production-coupled system by nitrogenase of R. sphaeroides and proton pump of H. salinarum. Graphical abstract


Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1031
Author(s):  
Maciej Spychalski ◽  
Rafal Kukawka ◽  
Włodzimierz Krzesiński ◽  
Tomasz Spiżewski ◽  
Monika Michalecka ◽  
...  

Triggering the plant resistance induction phenomenon by chemical compounds, for example acibenzolar-S-methyl ester, has been known and described in scientific literature. Other benzothadiazole derivatives have been also described; however, their properties have not been sufficiently studied. The tested substance, N-methyl-N-methoxyamide-7-carboxybenzo(1.2.3)thiadiazole (BTHWA), is an amide derivative of benzothiadiazole, showing a stimulating effect on plant growth, apart from its plant resistance inducing activity. This article presents the impact of BTHWA, used solo and in the program with fungicides, on the strawberry plants development, fruits health, yield, and quality parameters of the crop. The results show that the combined use of BTHWA and fungicides had a positive impact on the plants health and fruit health and nutraceutical and nutritional composition of compounds when compared to the results obtained when strawberries were treated only with the BTHWA or the fungicide. As a result of BTHWA use, the partitioning of assimilates has changed, which directly translated into the results of the conducted experiments. A reduction in the respiration of the fruit during storage was also observed, possibly due to a reduced disease infestation and a lower dry matter content in the fruit. A correlation between the parameters determined during the experiment was found. The BTHWA mode of action was evidenced to be beneficial to strawberry plants and fruit.


Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 728
Author(s):  
Eguzkine Ochoa

Assisted reproductive technologies (ART) are the treatment of choice for some infertile couples and even though these procedures are generally considered safe, children conceived by ART have shown higher reported risks of some perinatal and postnatal complications such as low birth weight, preterm birth, and childhood cancer. In addition, the frequency of some congenital imprinting disorders, like Beckwith–Wiedemann Syndrome and Silver–Russell Syndrome, is higher than expected in the general population after ART. Experimental evidence from animal studies suggests that ART can induce stress in the embryo and influence gene expression and DNA methylation. Human epigenome studies have generally revealed an enrichment of alterations in imprinted regions in children conceived by ART, but no global methylation alterations. ART procedures occur simultaneously with the establishment and maintenance of imprinting during embryonic development, so this may underlie the apparent sensitivity of imprinted regions to ART. The impact in adulthood of imprinting alterations that occurred during early embryonic development is still unclear, but some experimental evidence in mice showed higher risk to obesity and cardiovascular disease after the restriction of some imprinted genes in early embryonic development. This supports the hypothesis that imprinting alterations in early development might induce epigenetic programming of metabolism and affect long-term health. Given the growing use of ART, it is important to determine the impact of ART in genomic imprinting and long-term health.


2015 ◽  
Vol 88 (2) ◽  
pp. 116-127 ◽  
Author(s):  
Chloe C. Rings ◽  
Anthony R. Rafferty ◽  
Michael L. Guinea ◽  
Richard D. Reina

2021 ◽  
Author(s):  
Emily Rowlands ◽  
Tamara Galloway ◽  
Matthew Cole ◽  
Ceri Lewis ◽  
Victoria Peck ◽  
...  

<p>Antarctic krill (<em>Euphausia superba</em>), hereafter krill, are pivotal to the Antarctic marine ecosystem, forming the base of a highly productive system and contributing significantly to the biogeochemical cycle. The negative effects of anthropogenic climate stressors amplified in the Southern Ocean such as rapid warming and ocean acidification (OA) have been acknowledged for krill. Less explored is the impact of increasing plastic pollution, particularly in conditions that reflect the likely future Southern Ocean environment. We hypothesise that krill have heightened vulnerability to multi-stressor scenarios due to their physiological and behavioural traits coupled with rapid environmental changes of their Antarctic habitats. Here, we investigate the single and combined effects of nanoplastic (NP; spherical, aminated (NP-NH<sub>2</sub>), yellow-green, fluorescent polystyrene nanoparticles) and OA (pCO<sub>2</sub>-manipulated seawater, pH 7.7) on the embryonic development of krill eggs. Krill were collected in the Scotia Sea within the Atlantic sector of the Southern Ocean in austral summer 2019. Eggs from a single female were incubated in seawater at 0.5 °C for 6 days with three treatments: (i) with 0.16 μm NP, (ii) in acidified conditions, and (iii) with a combined treatment of NP (0.16μm) and acidification. All NP treatments were at a concentration of 2.5μg/ml. We found that exposure to the NP-OA multi-stress treatment negatively impacted the development of embryos, decreasing the probability of reaching the limb bud stage by 9% compared with the control, whilst no significant difference was observed for the singular NP or OA treatments. This preliminary study supports our hypothesis regarding the potential impacts of multiple stressors on vulnerable embryonic stages of this ecologically critical Antarctic species.</p>


Cancers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1756
Author(s):  
Jessica Guerra ◽  
Paola Chiodelli ◽  
Chiara Tobia ◽  
Claudia Gerri ◽  
Marco Presta

Primary cilium drives the left-right asymmetry process during embryonic development. Moreover, its dysregulation contributes to cancer progression by affecting various signaling pathways. The fibroblast growth factor (FGF)/FGF receptor (FGFR) system modulates primary cilium length and plays a pivotal role in embryogenesis and tumor growth. Here, we investigated the impact of the natural FGF trap long-pentraxin 3 (PTX3) on the determination of primary cilium extension in zebrafish embryo and cancer cells. The results demonstrate that down modulation of the PTX3 orthologue ptx3b causes the shortening of primary cilium in zebrafish embryo in a FGF-dependent manner, leading to defects in the left-right asymmetry determination. Conversely, PTX3 upregulation causes the elongation of primary cilium in FGF-dependent cancer cells. Previous observations have identified the PTX3-derived small molecule NSC12 as an orally available FGF trap with anticancer effects on FGF-dependent tumors. In keeping with the non-redundant role of the FGF/FGR system in primary cilium length determination, NSC12 induces the elongation of primary cilium in FGF-dependent tumor cells, thus acting as a ciliogenic anticancer molecule in vitro and in vivo. Together, these findings demonstrate the ability of the natural FGF trap PTX3 to exert a modulatory effect on primary cilium in embryonic development and cancer. Moreover, they set the basis for the design of novel ciliogenic drugs with potential implications for the therapy of FGF-dependent tumors.


2013 ◽  
Vol 8 (2) ◽  
pp. 155892501300800 ◽  
Author(s):  
Abdul Jabbar ◽  
Tanveer Hussain ◽  
Abdul Moqeet

The impact of card cylinder speed, card production rate and draw frame doubling on cotton yarn quality parameters was investigated by using the Box-Behnken experimental design. It was found that yarn tenacity, elongation and hairiness increase by increasing the number of draw frame doubling up to a certain level and then decrease by further increase in doubling. Yarn unevenness increased by increasing card production rate and total yarn imperfections increased by decreasing card cylinder speed and increasing card production rate.


2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Asha Kaur ◽  
Peter Scarborough ◽  
Mike Rayner

AbstractHealth-related claims (HRCs) are statements found on food packets that convey the nutritional quality of a food (nutrition claims) and/or its impact on a health outcome (health claims). Foods carrying HRCs have a slightly improved nutritional profile than foods without HRCs, however, it's unclear whether this translates into dietary improvements. We conducted a modelling study to measure the effect of HRCs on diet. As HRCs are already present on foods it is assumed that any impact that they have upon diet are already in effect. We modelled the impact on food purchases of removing HRCs, by assuming that the sales boost they receive is neutralised. These results can be inverted to estimate the current dietary impact of HRCs. Using the Living Costs Food (LCF) survey data, we calculate the average purchases and nutrient intake per person, per day. The LCF data is divided into sales of products with HRCs and sales of products without HRCs through solving mathematical equations combining LCF sales data with odds ratios from a meta-analysis examining the impact of HRCs on choices and data from a survey of foods examining the prevalence of HRCs and the nutritional quality of foods that carry them so that the sum of the sales of products with HRCs and without HRCs is equal to the total sales of products. Similarly, mathematical equations are solved that combine nutritional composition data with the sales of foods carrying and not carrying HRCs. In the baseline scenario foods carrying HRCs made-up 37% of the total purchases, and contributed 29% (559kcal) of the total kcals purchased (1907kcal). When HRCs are removed from foods there is an average increase of 18kcal/d (95% Uncertainty Intervals [UI] -15, 52), + 2g/d increase in total fat (95% UI -1, 4) and saturated fat (95% UI 1, 3), smaller changes are seen for protein (+ 0.5g/d, 95% UI -1, 2), total sugar (+ 0.5g/d, 95% UI -4, 7) and carbohydrate (-0.5g/d, 95% UI -5, 7). There is reduction in the amount of fruit (-11g/d, 95% UI -34, 26) but an increase in vegetables (+ 6g/d, 95% UI -6, 19). These results should be interpreted with caution due to the large uncertainty intervals. When HRCs are removed, we see a small deterioration in the quality of the average diet. If we invert these findings we can assume HRCs currently have a positive, albeit small, impact on diet.


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