Novel GUS expression patterns following transposition of an enhancer trap Ds element in Arabidopsis

1995 ◽  
Vol 249 (4) ◽  
pp. 357-365 ◽  
Author(s):  
V. I. Klimyuk ◽  
L. Nussaume ◽  
K. Harrison ◽  
J. D. G. Jones
Keyword(s):  
Expression Patterns ◽  
Gus Expression ◽  
Enhancer Trap ◽  
Ds Element

Functional analysis of GUS expression patterns and T-DNA integration characteristics in rice enhancer trap lines

Plant Science ◽  
2005 ◽  
Vol 168 (6) ◽  
pp. 1571-1579 ◽  
Author(s):  
Hao Peng ◽  
Hongmei Huang ◽  
Yongzhi Yang ◽  
Ying Zhai ◽  
Jinxia Wu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Expression Patterns ◽  
Gus Expression ◽  
Enhancer Trap ◽  
Dna Integration ◽  
Enhancer Trap Lines

The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells

Development ◽  
1997 ◽  
Vol 124 (4) ◽  
pp. 761-771 ◽  
Author(s):  
K. Ito ◽  
W. Awano ◽  
K. Suzuki ◽  
Y. Hiromi ◽  
D. Yamamoto
Keyword(s):  
Glial Cells ◽  
Sensory Integration ◽  
Mushroom Body ◽  
Expression Patterns ◽  
Cell Lineage ◽  
Cell Types ◽  
Enhancer Trap ◽  
Adult Brain ◽  
A New Technique ◽  
Lineage Analysis

The mushroom body (MB) is an important centre for higher order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the Flippase (flp)/FRT system and the GAL4/UAS system. We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure. The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in all four of the MBNb lineages. Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures.

scholarly journals Identification of Flower-Specific Promoters through Comparative Transcriptome Analysis in Brassica napus

2019 ◽  
Vol 20 (23) ◽  
pp. 5949 ◽  
Author(s):  
Yan Li ◽  
Caihua Dong ◽  
Ming Hu ◽  
Zetao Bai ◽  
Chaobo Tong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Genes ◽  
Oilseed Rape ◽  
Agronomic Traits ◽  
Expression System ◽  
Expression Patterns ◽  
Gus Expression ◽  
Pcr Analysis ◽  
Transcriptome Data ◽  
Sclerotinia Stem Rot

Brassica napus (oilseed rape) is an economically important oil crop worldwide. Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is a threat to oilseed rape production. Because the flower petals play pivotal roles in the SSR disease cycle, it is useful to express the resistance-related genes specifically in flowers to hinder further infection with S. sclerotiorum. To screen flower-specific promoters, we first analyzed the transcriptome data from 12 different tissues of the B. napus line ZS11. In total, 249 flower-specific candidate genes with high expression in petals were identified, and the expression patterns of 30 candidate genes were verified by quantitative real-time transcription-PCR (qRT-PCR) analysis. Furthermore, two novel flower-specific promoters (FSP046 and FSP061 promoter) were identified, and the tissue specificity and continuous expression in petals were determined in transgenic Arabidopsis thaliana with fusing the promoters to β-glucuronidase (GUS)-reporter gene. GUS staining, transcript expression pattern, and GUS activity analysis indicated that FSP046 and FSP061 promoter were strictly flower-specific promoters, and FSP046 promoter had a stronger activity. The two promoters were further confirmed to be able to direct GUS expression in B. napus flowers using transient expression system. The transcriptome data and the flower-specific promoters screened in the present study will benefit fundamental research for improving the agronomic traits as well as disease and pest control in a tissue-specific manner.

scholarly journals Enhancer Trap Expression Patterns Provide a Novel Teaching Resource

2002 ◽  
Vol 130 (4) ◽  
pp. 1747-1753 ◽  
Author(s):  
Matt Geisler ◽  
Barbara Jablonska ◽  
Patricia S. Springer
Keyword(s):  
Expression Patterns ◽  
Enhancer Trap ◽  
Teaching Resource

scholarly journals Subdivision of the drosophila mushroom bodies by enhancer-trap expression patterns

Neuron ◽  
1995 ◽  
Vol 15 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Ming Yao Yang ◽  
J.Douglas Armstrong ◽  
Ilya Vilinsky ◽  
Nicholas J. Strausfeld ◽  
Kim Kaiser
Keyword(s):  
Expression Patterns ◽  
Enhancer Trap ◽  
Mushroom Bodies

Expression patterns of cytoplasmic pyruvate, orthophosphate dikinase of rice (C3) and maize (C4) in a C3 plant, rice

2000 ◽  
Vol 27 (4) ◽  
pp. 343 ◽  
Author(s):  
Mika Nomura ◽  
Naoki Sentoku ◽  
Shigeyuki Tajima ◽  
Makoto Matsuoka
Keyword(s):  
Expression Patterns ◽  
Gus Expression ◽  
C4 Plants ◽  
C3 And C4 Plants ◽  
C3 Plant ◽  
Pyruvate Orthophosphate Dikinase ◽  
Gus Reporter ◽  
Orthophosphate Dikinase ◽  
Organ Specific ◽  
Specific Localization

Two types of mRNAs are transcribed from the C4-type pyruvate, orthophosphate dikinase gene (Pdk) with different sizes, which encode chloroplastic and cytoplasmic forms of the enzyme. The two transcripts are pro duced by two independent promoters and this unusual dual promoter system is also found in the C4-like Pdk gene of the C3 plant, rice. In order to elucidate the expression pattern of the cytoplasmic transcript from the maize C4-type and rice C4-like Pdk genes, we have produced chimeric constructs with the ß-glucuronidase (GUS) reporter gene under the control of the cytoplasmic promoters and introduced the constructs into rice. Both cytoplasmic promoters directed GUS expression in non-photosynthetic organs, such as endosperm and roots, in transgenic rice plants, while expression was low in photosynthetic organs. These results indicate that the organ-specific localization of the cyto-plasmic enzyme is similar in C3 and C4 plants. The results also suggest the possibility that the cytoplasmic enzyme has a similar function(s) in non-photosynthetic organs both in C3 and C4 plants.

scholarly journals Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Hyeonso Ji ◽  
Sung-Ryul Kim ◽  
Yul-Ho Kim ◽  
Jung-Pil Suh ◽  
Hyang-Mi Park ◽  
...  
Keyword(s):  
Wild Rice ◽  
Insect Pest ◽  
Expression Patterns ◽  
Brown Planthopper ◽  
Introgression Line ◽  
Leaf Sheath ◽  
Gus Expression ◽  
Complementation Test ◽  
Vascular Bundles ◽  
Wild Rice Species

Abstract Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from the BPH-resistant introgression line derived from the wild rice species Oryza australiensis. Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein. BPH18 has two NBS domains, unlike the typical NBS-LRR proteins. The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks. The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells. Whole genome sequencing of the near-isogenic lines (NILs), NIL-BPH18 and NIL-BPH26, revealed that BPH18 located at the same locus of BPH26. However, these two genes have remarkable sequence differences and the independent NILs showed differential BPH resistance with different expression patterns of plant defense-related genes, indicating that BPH18 and BPH26 are functionally different alleles. These findings would facilitate elucidation of the molecular mechanism of BPH resistance and the identified novel alleles to fast track breeding BPH resistant rice cultivars.

scholarly journals High-resolution analysis of central nervous system expression patterns in zebrafish Gal4 enhancer-trap lines

2015 ◽  
Vol 244 (6) ◽  
pp. 785-796 ◽  
Author(s):  
Hideo Otsuna ◽  
David A. Hutcheson ◽  
Robert N. Duncan ◽  
Adam D. McPherson ◽  
Aaron N. Scoresby ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
High Resolution ◽  
Expression Patterns ◽  
Enhancer Trap ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Enhancer Trap Lines

scholarly journals A Novel Drosophila Alkaline Phosphatase Specific to the Ellipsoid Body of the Adult Brain and the Lower Malpighian (Renal) Tubule

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 285-297 ◽  
Author(s):  
Ming Yao Yang ◽  
Zongsheng Wang ◽  
Matthew MacPherson ◽  
Julian A T Dow ◽  
Kim Kaiser
Keyword(s):  
Alkaline Phosphatase ◽  
Expression Patterns ◽  
Fluid Secretion ◽  
Regulatory Elements ◽  
Enhancer Trap ◽  
Adult Brain ◽  
P Element ◽  
Renal Tubules ◽  
Genomic Locus ◽  
Ellipsoid Body

Abstract Two independent Drosophila melanogaster P{GAL4} enhancer-trap lines revealed identical GAL4-directed expression patterns in the ellipsoid body of the brain and in the Malpighian (renal) tubules in the abdomen. Both P-element insertions mapped to the same chromosomal site (100B2). The genomic locus, as characterized by plasmid rescue of flanking DNA, restriction mapping, and DNA sequencing, revealed the two P{GAL4} elements to be inserted in opposite orientations, only 46 bp apart. Three genes flanking the insertions have been identified. Calcineurin A1 (previously mapped to 21E-F) lies to one side, and two very closely linked genes lie to the other. The nearer encodes Aph-4, the first Drosophila alkaline phosphatase gene to be identified; the more distant gene [l(3)96601] is novel, with a head-elevated expression, and with distant similarity to transcription regulatory elements. Both in situ hybridization with Aph-4 probes and direct histochemical determination of alkaline phosphatase activity precisely matches the enhancer-trap pattern reported by the original lines. Although the P-element insertions are not recessive lethals, they display tubule phenotypes in both heterozygotes and homozygotes. Rates of fluid secretion in tubules from c507 homozygotes are reduced, both basally, and after stimulation by CAP2b, cAMP, or Drosophila leucokinin. The P-element insertions also disrupt the expression of Aph-4, causing misexpression in the tubule main segment. This disruption extends to tubule pigmentation, with c507 homozygotes displaying white-like transparent main segments. These results suggest that Aph-4, while possessing a very narrow range of expression, nonetheless plays an important role in epithelial function.

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