A monoclonal antibody that detects myoepithelial cells in exocrine glands, basal cells in other epithelia and basal and suprabasal cells in certain hyperplastic tissues

1986 ◽  
Vol 409 (5) ◽  
pp. 725-738 ◽  
Author(s):  
J. Caselitz ◽  
B. Walther ◽  
J. Wustrow ◽  
G. Seifert ◽  
K. Weber ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Myoepithelial Cells ◽  
Exocrine Glands ◽  
Basal Cells

Characteristics of ruminant mammary epithelial cells grown in primary culture in serum-free medium

1992 ◽  
Vol 59 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Steven J. Winder ◽  
Alan Turvey ◽  
Isabel A. Forsyth
Keyword(s):  
Monoclonal Antibody ◽  
Mammary Epithelial Cells ◽  
Myoepithelial Cells ◽  
Mammary Epithelial ◽  
Serum Free ◽  
Commercial Medium ◽  
Attachment Factor ◽  
Rat Tail

SummaryCells were obtained from the mammary glands of sheep and cows by collagenase–hyaluronidase digestion. Characterization of cells as epithelial was by reaction with a monoclonal antibody to cytokeratin. A subpopulation of spindle-shaped or stellate cells reacted with a monoclonal antibody to desmin and may be related to myoepithelial cells. The development is described of a simple serum-free culture system for these cells on gels of rat tail (type 1) collagen. A commercial medium (M199) was used, buffered with Hepes and with bovine serum albumin as the sole protein supplement, plus fibronectin for the first 18 h only as an attachment factor. The cell cultures showed stimulated DNA synthesis in response to mitogens on attached gels and also responded as floating cultures to lactogenic hormones with production of α-lactalbumin.

Regeneration of mammary glands in vivo from isolated mammary ducts

Development ◽  
1986 ◽  
Vol 96 (1) ◽  
pp. 229-243
Author(s):  
E. Jane Ormerod ◽  
Philip S. Rudland
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Cell Types ◽  
Mammary Glands ◽  
Myoepithelial Cells ◽  
Basal Cells ◽  
Alveolar Cells ◽  
Terminal End Buds ◽  
Mammary Cell

Rat mammary ducts, free of buds, can alone regenerate complete mammary trees when transplanted into the interscapular fat pads of syngeneic host rats. All the main mammary cell types are identified within such outgrowths. Epithelial cells, which show the presence of milk fat globule membrane antigens and microvilli on their luminal surfaces, line the ducts. Basal cells surrounding the ducts show characteristic features of myoepithelial cells: immunoreactive actin and keratin within the cytoplasm, myofilaments, pinocytotic vesicles and hemidesmosomal attachments to the basement membrane. Cells within the end buds and lateral buds, however, show few if any cytoplasmic myofilaments and are relatively undifferentiated in appearance. Intermediate morphologies between these cells and myoepithelial cells are seen nearer the ducts. In this respect they exactly resemble the cap cells found in terminal end buds (TEBs) of normal mammary glands. Occasional epithelial cells within alveolar buds show the presence of immunoreactive casein, which is a product of secretory alveolar cells in the normal rat mammary gland. Dissected terminal end buds can regenerate similar ductal outgrowths. Thus, ductal tissue alone can generate all the major mammary cell types seen in the normal gland, including the cap cells.

scholarly journals A Murine Monoclonal Antibody (VM-1) Against Human Basal Cells Inhibits the Growth of Human Keratinocytes in Culture

1985 ◽  
Vol 84 (4) ◽  
pp. 257-261 ◽  
Author(s):  
Allan R. Oseroff ◽  
Eva A. Pfendt ◽  
Linda DiCicco ◽  
Vera B. Morhenn
Keyword(s):  
Monoclonal Antibody ◽  
Human Keratinocytes ◽  
Murine Monoclonal Antibody ◽  
Basal Cells

Expression of protein p27 in various forms of seborrheic keratosis

2016 ◽  
Vol 19 (5) ◽  
pp. 283-286
Author(s):  
Alexandra K. Aleksandrova ◽  
G. I Sukolin ◽  
V. A Smolynnikova
Keyword(s):  
Cell Cycle ◽  
Monoclonal Antibody ◽  
Kinase Inhibitor ◽  
Research Unit ◽  
Basal Cells ◽  
Seborrheic Keratosis ◽  
Histological Types ◽  
Characteristic Features ◽  
Definition Of ◽  
P27 Kip1

The significant role in the pathogenesis of seborrheic keratosis (SK) plays the violation of the cell cycle regulation. According to the research unit the cell proliferation in acanthotic and irritated histological types of SK is regulated by p27 (Kip1), cyclin-dependent kinase inhibitor. Considering the variety of histological types of SK, definition of p27 expression will reveal the characteristic features of the cell cycle disorders and proliferation for each type of tumor. Material and Methods. Of the 102 tumors from patients with SK, according to the results of histological examination, were selected 10 specimens of each histological types of SK: acanthotic, hyperkeratotic, adenoid, irritated, and clonal. We assessed all specimens for p27 (Kip1) expression using immunohistochemistry (monoclonal antibody p27 at a dilution of 1:20 (Novocastra Laboratories Ltd.). Three skin biopsy samples of healthy individuals were included. Results. Severe diffuse nuclear expression of p27 was present in all cases of adenoid, irritated, and 4 cases of clonal histological types of SK. In other tumor types positive reaction with monoclonal antibody to p27 was reduced as compared with healthy skin were recorded single positively stained nuclei of basal cells. Conclusions. Thus, we have found a violation of p27 protein expression in all types of seborrheic keratosis as with the excess (adenoid, irritated, clonal SK type) and in p27 protein deficiency (acanthotic, hyperkeratotic) normal course of cell cycle phases is broken. This leads to the disappearance of control over the cell proliferative activity and apoptosis, facilitating a slow, uncontrolled growth of SK cells.

scholarly journals Immunolocalization of the Smooth Muscle-Specific Protein Calponin in Complex and Mixed Tumors of the Mammary Gland of the Dog: Assessment of the Morphogenetic Role of the Myoepithelium

2002 ◽  
Vol 39 (2) ◽  
pp. 247-256 ◽  
Author(s):  
A. Espinosa de los Monteros ◽  
M. Y. Millán ◽  
J. Ordás ◽  
L. Carrasco ◽  
C. Reymundo ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Smooth Muscle ◽  
Mammary Gland ◽  
Malignant Tumors ◽  
Staining Intensity ◽  
Myoepithelial Cells ◽  
Specific Protein ◽  
Benign Tumors ◽  
Normal Mammary Gland ◽  
Mixed Tumors

The immunohistochemical expression of the smooth muscle-specific protein calponin was studied to assess the contribution of myoepithelial cells to the histogenesis of spindle cells of complex and mixed tumors of the mammary gland of the dog and the origin of cartilage and bone in mixed tumors. Formalin-fixed tissues from 55 benign and malignant tumors (49 also containing surrounding normal mammary gland) were evaluated. Periacinar and periductal myoepithelial cells of all the 49 normal mammary glands were diffusely stained by the anti-human calponin monoclonal antibody. Calponin was found in 53 (98%) of the tumors studied, reacting with the myoepithelium-like cells of 86% of benign tumors and their remnants in 85% of malignant tumors. Five different types of calponin-immunoreactive myoepithelial cells were identified: hypertrophic myoepithelial cells, fusiform cells, stellate myoepithelial cells, rounded (myoepithelial) cells, and chondroblasts. Differences in staining intensity and staining pattern among these five types of cells suggested a transition of myoepithelial cells to chondroblasts. Stromal myofibroblasts also showed calponin immunoreactivity, but they did not react with a cytokeratin 14 monoclonal antibody, which recognizes myoepithelial cells in mammary gland. Calponin appears to be a very sensitive marker of normal and neoplastic myoepithelium in the canine mammary gland, and its identification in different cell types of complex and mixed tumors of the mammary gland of the dog suggests a major histogenetic role for myoepithelial cells.

scholarly journals Development of a Novel Monoclonal Antibody Recognizing Basal Cells of Human Squamous Epithelia.

2002 ◽  
Vol 65 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Jiro HITOMI ◽  
Fumio ISHIZAKI ◽  
Eiji KIMURA ◽  
Nobuyuki SATO
Keyword(s):  
Monoclonal Antibody ◽  
Basal Cells ◽  
Squamous Epithelia

scholarly journals A monoclonal antibody against basal cells of human epidermis. Potential use in the diagnosis of cervical neoplasia.

1985 ◽  
Vol 76 (5) ◽  
pp. 1978-1983 ◽  
Author(s):  
V B Morhenn ◽  
A B Schreiber ◽  
O Soriero ◽  
W McMillan ◽  
A C Allison
Keyword(s):  
Monoclonal Antibody ◽  
Cervical Neoplasia ◽  
Human Epidermis ◽  
Basal Cells ◽  
Potential Use

Characterization of luminal and basal cells flow-sorted from the adult rat mammary parenchyma

1991 ◽  
Vol 100 (3) ◽  
pp. 459-471
Author(s):  
S.R. Dundas ◽  
M.G. Ormerod ◽  
B.A. Gusterson ◽  
M.J. O'Hare
Keyword(s):  
Neutral Endopeptidase ◽  
Myoepithelial Cells ◽  
Basal Cells ◽  
Proliferating Cells ◽  
Adult Rat ◽  
Morphological Criteria ◽  
Membrane Antigens ◽  
Cell Type Specific ◽  
Alpha Actin ◽  
Type 3

Differentially expressed membrane antigens have been used to flow-sort viable luminal epithelial and myoepithelial cells from freshly disaggregated adult virgin rat mammary parenchyma. Resulting cultures and clones have been characterized morphologically and by a panel of antibodies that recognise cell-type-specific cytoskeletal antigens in the intact mammary gland. Five clonal phenotypes were recognisable by morphological criteria, three (types 1–3) exclusively associated with sorted luminal epithelial (25.5-positive) cells, and two (types 4 and 5) generated from the sorted myoepithelial (CALLA/neutral endopeptidase 24.11-positive) cells. All clones derived from myoepithelial cells continued to express a basal parenchymal marker in the form of the rat equivalent of human cytokeratin 14, while smooth muscle alpha-actin was expressed by the small slowly growing type 4 clones but was found in fewer cells in rapidly proliferating type 5 myoepithelially derived clones. Two of the luminal clone types, characterized by an attenuated appearance and slow growth (types 1/2), expressed only luminal-specific markers, including the equivalent of human cytokeratins 7/18/19. Type 3 clones, by contrast, consisted of rapidly proliferating cells, many of which either co-expressed CK14 and CK18 antigens (type 3a) or were composed of a mosaic of CK14+/CK18-, CK14+/CK18+ and CK14-/CK18+ cells (type 3b). The sorted myoepithelially derived clones grew faster than clones from sorted luminal cells as evidenced by the larger fraction of cells synthesizing DNA. All types of clone could be obtained from both isolated ducts and alveoli, when these were cloned separately, although there were some differences in their relative frequency.(ABSTRACT TRUNCATED AT 250 WORDS)

A new monoclonal antibody to a cell-surface antigen that distinguishes luminal epithelial and myoepithelial cells in the rat mammary gland

1989 ◽  
Vol 94 (3) ◽  
pp. 545-552
Author(s):  
R.S. Mahendran ◽  
M.J. O'Hare ◽  
M.G. Ormerod ◽  
P.A. Edwards ◽  
R.A. McIlhinney ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Milk Fat ◽  
Single Cells ◽  
Myoepithelial Cells ◽  
Stages Of Development ◽  
Leukaemia Cell Line ◽  
Flow Cytometric ◽  
Rat Mammary Gland

A monoclonal antibody (25.5) has been produced that recognises luminal epithelial cells of the rat mammary gland. This antibody together with monoclonal anti-CALLA antibodies, which react with mammary myoepithelial cells, has been used in biochemical, immunocytochemical and flow cytometric studies. Antibody 25.5 bound to proteins of molecular weight 70K and 25K (K = 10(3) Mr) in both the rat milk fat globule membrane and in single cell suspensions prepared from the virgin adult rat mammary gland. Anti-CALLA antibody (J5), recognised a 93–100K protein in the gland extracts, which co-electrophoresed with the CALLA/CD-10 antigen from NALM-6 acute lymphoblastic leukaemia cell line. Antibody 25.5 bound to the luminal surface of rat mammary epithelial cells at all stages of development from neonatal through to pregnancy, lactation and involution. CALLA immunoreactive staining has previously been shown on basally located presumptive myoepithelial cells at all stages of development. Flow cytometric analyses demonstrated that 25.5 and anti-CALLA antibodies stained independent cell populations in suspensions of single cells prepared from purified epithelial elements from the mammary gland of adult virgin rat.

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