Membrane specializations in the first optic neuropil of the housefly,Musca domestica L. II. Junctions between glial cells

1980 ◽  
Vol 9 (4) ◽  
pp. 451-469 ◽  
Author(s):  
Che Chi ◽  
Stanley D. Carlson

Author(s):  
V. F. Allison ◽  
R. S. Sohal

Relatively little is known regarding the structure and function of glial cells in the brain of invertebrate organisms. Fine structural aspects of the interrelationships between glial cells and neurons in the brain of the housefly are described. Three types of glial cells have been identified in the brain of the housefly. Cell bodies of the neurons and two glial cell types are located at the periphery of the brain and surround a centrally located neuropil. Soma of the neurons are completely surrounded by a single or multiple layers of glioplasm which precludes the existence of synaptic sites on the cell body (Fig. 1). Synaptic sites are restricted to the neuropil region. Thin processes of glioplasm also invaginated the perikaryon.





Author(s):  
Wiktor Djaczenko ◽  
Carmen Calenda Cimmino

The simplicity of the developing nervous system of oligochaetes makes of it an excellent model for the study of the relationships between glia and neurons. In the present communication we describe the relationships between glia and neurons in the early periods of post-embryonic development in some species of oligochaetes.Tubifex tubifex (Mull. ) and Octolasium complanatum (Dugès) specimens starting from 0. 3 mm of body length were collected from laboratory cultures divided into three groups each group fixed separately by one of the following methods: (a) 4% glutaraldehyde and 1% acrolein fixation followed by osmium tetroxide, (b) TAPO technique, (c) ruthenium red method.Our observations concern the early period of the postembryonic development of the nervous system in oligochaetes. During this period neurons occupy fixed positions in the body the only observable change being the increase in volume of their perikaryons. Perikaryons of glial cells were located at some distance from neurons. Long cytoplasmic processes of glial cells tended to approach the neurons. The superimposed contours of glial cell processes designed from electron micrographs, taken at the same magnification, typical for five successive growth stages of the nervous system of Octolasium complanatum are shown in Fig. 1. Neuron is designed symbolically to facilitate the understanding of the kinetics of the growth process.



Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).



Author(s):  
R.V.W. Dimlich ◽  
M.H. Biros

Although a previous study in this laboratory determined that Purkinje cells of the rat cerebellum did not appear to be damaged following 30 min of forebrain ischemia followed by 30 min of reperfusion, it was suggested that an increase in rough endoplasmic reticulum (RER) and/or polysomes had occurred in these cells. The primary objective of the present study was to morphometrically determine whether or not this increase had occurred. In addition, since there is substantial evidence that glial cells may be affected by ischemia earlier than other cell types, glial cells also were examined. To ascertain possible effects on other cerebellar components, granule cells and neuropil near Purkinje cells as well as neuropil in the molecular layer also were evaluated in this investigation.



Author(s):  
Roy J. Baerwald ◽  
Lura C. Williamson

In arthropods the perineurium surrounds the neuropile, consists of modified glial cells, and is the morphological basis for the blood-brain barrier. The perineurium is surrounded by an acellular neural lamella, sometimes containing scattered collagen-like fibrils. This perineurial-neural lamellar complex is thought to occur ubiquitously throughout the arthropods. This report describes a SEM and TEM study of the sheath surrounding the ventral nerve cord of Panulirus argus.Juvenile P. argus were collected from the Florida Keys and maintained in marine aquaria. Nerve cords were fixed for TEM in Karnovsky's fixative and saturated tannic acid in 0.1 M Na-cacodylate buffer, pH = 7.4; post-fixed in 1.0% OsO4 in the same buffer; dehydrated through a graded series of ethanols; embedded in Epon-Araldite; and examined in a Philips 200 TEM. Nerve cords were fixed for SEM in a similar manner except that tannic acid was not used.



2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies



2020 ◽  
pp. 28-30
Author(s):  
Mikhail A. Levchenko ◽  

The control of houseflies (Musca domestica L.) in veterinary surveillance premises is an important measure for the welfare of animals against infectious and invasive diseases. For this purpose, the most effective chemical insecticides are used. To prevent possible resistance to them, bait insecticides with two active binary ingredients from different chemical classes are used. The work was carried out in the laboratory of the Tyumen Scientific Center and in the production conditions of ZAO Pyshminskaya Poultry Farm. This material presents the main stages in the development of a prototype of the insecticidal bait Mukhnet AX containing two insecticides: 1.5% acetamiprid and 6% chlorfenapir. For this purpose, adults of houseflies Musca domestica L. 3-5 days old were used. Under laboratory conditions, effective rational doses of the above insecticides were determined by group feeding, feeding and by the method of assessing food insecticidal baits when fighting flies from 0.00002 to 4% concentrations. According to the results of laboratory studies, it was found that the optimal doses causing 100% death of insects were 0.5% for acetamiprid and 2% for chlorfenapir. The insecticidal efficiency of the developed bait Mukhnet AH against flies in production conditions on the first day after the treatment of the livestock building was 91.25%. The insecticidal effect of the measures taken lasted for at least 6 days. The restoration of the number to the previous level of the number of insects occurred after 10 days. Based on the results obtained, the Method of using the composition of an insecticidal bait agent in the fight against Musca domestica was proposed and patented.



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