Stimulation of proliferative activity of human natural killers (CD16+CD56+ cells) by recombinant interleukin-3in vitro

1995 ◽  
Vol 119 (4) ◽  
pp. 396-399 ◽  
Author(s):  
S. B. Cheknev
Keyword(s):  
Proliferative Activity ◽  
Natural Killers ◽  
Stimulation Of

scholarly journals STUDIES ON X-RAY EFFECTS

1920 ◽  
Vol 31 (1) ◽  
pp. 13-29 ◽  
Author(s):  
Waro Nakahara ◽  
James B. Murphy
Keyword(s):  
Lymphoid Tissue ◽  
Proliferative Activity ◽  
X Rays ◽  
X Ray ◽  
Lymph Glands ◽  
Spark Gap ◽  
Mitotic Figures ◽  
Ray Effects ◽  
Stimulation Of

A dose of x-rays governed by the following factors induces a stimulation of lymphoid tissue in mice: spark-gap ⅞ inch, milliamperage 25, distance 8 inches, time of exposure 10 minutes. Within 4 days after this dose there appeared an abnormally large number of mitotic figures in the lymphoid tissue of spleen and lymph glands, indicating an acceleration of the proliferative activity of the tissue.

scholarly journals The Development of a Technique for Sensitizing Cells of Various Origins for Biotechnological Purposes

2022 ◽  
Author(s):  
Anna Oleshkevich ◽  
Elena Yarygina
Keyword(s):  
Cell Culture ◽  
Traveling Wave ◽  
Proliferative Activity ◽  
Modulation Frequency ◽  
Mdbk Cell ◽  
Proliferation Index ◽  
Frequency Range ◽  
Generation Frequency ◽  
Modulated Waves ◽  
Stimulation Of

The functional activity stimulation of cell cultures was tested in MDBK cell culture, photobacteria AliivibriofischeriandHalobacteriumhalobium. Theaim of the investigation was to increase the ”yield” of the cells using an environmentallysafe stimulant and membrane-tropic agent that isalso safe for the experimenter. Ultrasonicwaves were used.Experimental ultrasonic exposure varied within the following limits: time from 1 to 300 sec, SATA-intensity of 0.01–2.0 W/cm2, generation frequency of 0.88 or 2.64 MHz, standing or traveling wave. The modulation frequency range was within 0.1–150 Hz. The devices used were: UST-1-01F, UST-5 and UST1.02C. The modulating generators were G3–112 and CP–110.Stimulation of MDBK cell growth was initiated by US-intensity of 0.03–0.05 W/cm2 , with an exposure of 5–30 sec.Exposure to ultrasound with an intensity of 0.2–0.4 W/cm2 (for 3 min) had a stimulating effect on bioluminescence and was associated with an increase in the growth rate ofA. fischeri. The findings indicated that 0.4 W/cm2ultrasonic intensity and modulation frequencies from 0.25 to 0.7 Hz can stimulate the growth of archaea.It was revealed that the maximum proliferation index in all cases of stimulant application was noted in cultures with minimal initial proliferative activity in the control.The authors expect thatthese results on the possibilities of acoustic continuous and modulated waves can be applied for biotechnological purposes to develop a new biotechnological method. Keywords: cell culture, ultrasound, proliferation, stimulation

scholarly journals EFFECT OF INTERLEUKINS 2, 7, 15 ON THE PROLIFERATION OF NATURAL KILLERS IN VITRO

2021 ◽  
Vol 20 (1) ◽  
pp. 56-66
Author(s):  
A. О. Sitkovskaya ◽  
E. Yu. Zlatnik ◽  
S. Yu. Filippova ◽  
E. S. Bondarenko ◽  
L. N. Vaschenko ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Mononuclear Cells ◽  
Locally Advanced ◽  
Proliferation Index ◽  
Natural Killers ◽  
Killer Cells ◽  
Stimulation Of

Introduction. The actual task of modern adoptive cancer immunotherapy is the selection of the optimal composition of cytokines for ex vivo stimulation of immunocompetent cells for subsequent administration to oncological patients.The purpose of the study was to compare the effect of interleukin (IL) 2, 7, 15 and their combinations on the proliferation of natural killer cells in breast cancer (BC) patients in vitro.Materials and methods. The research was conducted on natural killer cells isolated by magnetic separation from mononuclear cells of peripheral blood of ten patients with locally advanced BC (stage II). After separation, the cells were cultured at a concentration of 2.5 × 105 cells / ml for 10 days in RPMI 1640 medium supplemented with cytokines at a concentration of 40 ng / ml each in five experimental variants: IL‑2; IL‑7; IL‑15; IL‑7 / IL‑15; IL‑2 / IL‑7 / IL‑15. On the 10th day of cultivation, the phenotype of cells and the cell cycle were studied by flow cytometry. For immunophenotyping of cells, we used monoclonal antibodies to antigens: CD3, CD16 / 56, CD45, CD4, CD19, and CD8. For cell cycle study cells were stained with propidium iodide.Results. On the final 10th day of cultivation the number of living cells expressed as percentage of the seeding numbers were significantly different from control (45.9 %) in samples IL‑2 (86.8 %) and IL‑7 / IL‑15 (85.6 %), IL‑15 (76.4 %), IL‑2 / IL‑7 / IL‑15 (75.8 %). The proportion of natural killers (CD16+CD56+) significantly differed from the control (18.2 %) in samples IL‑2 (45.6 %), IL‑15 (39.9 %), IL‑7 / IL‑15 (36.2 %), IL‑2 / IL‑7 / IL‑15 (35.9 %). The propor‑ tion of natural killer T cells (CD3+ / CD16+CD56+) significantly differed from the control (0.4 %) in samples incu‑ bated with IL‑2 (2.06 %), IL‑15 (2.2 %), IL‑7 (0.9 %), IL‑7 / IL‑15 (1.26 %), IL‑2 / IL‑7 / IL‑15 (2.46 %). All experimental tests also showed a significant increase in the proportion of cells in the S‑phase and increase in the proliferation index (G2 / M + S).Conclusion. The maximum stimulation of the proliferation of natural killer cells isolated from the blood of patients with BC in vitro was obtained by stimulation with IL‑15 alone and in combinations with γc‑cytokines.

scholarly journals ANTI-HISTONE H1 IgGs POSSESS PROLIFERATIVE ACTIVITY TOWARDS HUMAN T-LEUKAEMIA CEM CELLS

2017 ◽  
Vol 39 (1) ◽  
pp. 36-41
Author(s):  
Yu Kit ◽  
I Magorivska ◽  
R Bilyi ◽  
S Myronovskij ◽  
R Stoika
Keyword(s):  
Lupus Erythematosus ◽  
Proliferative Activity ◽  
Serum Proteins ◽  
Gel Filtration ◽  
Histone H1 ◽  
Cross Reactivity ◽  
Acidic Ph ◽  
Significant Stimulation ◽  
Positively Charged ◽  
Stimulation Of

The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens.

Leukaemia inhibitory factor stimulates proliferation of prospermatogonial stem cells

1997 ◽  
Vol 9 (7) ◽  
pp. 717 ◽  
Author(s):  
Diana B. Nikolova ◽  
Yordanka S. Martinova ◽  
Martin Seidensticker ◽  
Anthony R. Bellvé
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Proliferative Activity ◽  
Inhibitory Factor ◽  
Leukaemia Inhibitory Factor ◽  
Synthetic Activity ◽  
Local Regulation ◽  
Rat Testis ◽  
A Cell ◽  
Stimulation Of

The effect of leukaemia inhibitory factor (LIF) on the proliferation of prospermatogonial stem cells was testedin vitro. Pieces of 3-day-old rat testis were cultured in the presence of a range of doses of LIF alone or in combination with 1 ng mL -1 seminiferous growth factor (SGF). Stimulation of the proliferative activity of quiescent prospermatogonia was detected immunocytochemically with a cell proliferation kit. After 24 h culture, LIF signicantly increased the percentage of labelled prospermatogonia, with the peak of activity at 20 pg mL -1 . The combination of LIF and SGF resulted in a decrease in DNA synthetic activity of the germ cells. Hence, LIF and SGF play a role in local regulation at the onset of spermatogenesis in the rat testis.

scholarly journals Электростимуляция дермальных фибробластов человека на электропроводящей матрице

2021 ◽  
Vol 91 (12) ◽  
pp. 2059
Author(s):  
К.А. Колбе ◽  
М.А. Шишов ◽  
И.Ю. Сапурина ◽  
Н.В. Смирнова ◽  
В.В. Кодолова-Чухонцева ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Proliferative Activity ◽  
Human Fibroblasts ◽  
Dermal Fibroblasts ◽  
Single Wall Carbon Nanotubes ◽  
Human Dermal Fibroblasts ◽  
Intact Cells ◽  
Conducting Composite ◽  
The Matrix ◽  
Stimulation Of

Conducting composite based on biocompatible chitosan and single wall carbon nanotubes was used as a matrix for electrical stimulation of human fibroblasts. Parameters of ionic and electronic currents passing through the matrix upon applying cyclic potentials (±100 mV) were studied; the scaffold demonstrated high stability in the course of prolonged electric cycling. It was shown that preliminary electrical stimulation facilitated proliferative activity of human dermal fibroblasts in comparison to that of intact cells.

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

1971 ◽  
Vol 29 ◽  
pp. 570-571
Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman
Keyword(s):  
Chemical Composition ◽  
Rat Liver ◽  
Future Study ◽  
Mode Of Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Effective Inhibitor ◽  
Biochemical Effects ◽  
Glycerophosphate Dehydrogenase ◽  
Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

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