scholarly journals Электростимуляция дермальных фибробластов человека на электропроводящей матрице

2021 ◽  
Vol 91 (12) ◽  
pp. 2059
Author(s):  
К.А. Колбе ◽  
М.А. Шишов ◽  
И.Ю. Сапурина ◽  
Н.В. Смирнова ◽  
В.В. Кодолова-Чухонцева ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Proliferative Activity ◽  
Human Fibroblasts ◽  
Dermal Fibroblasts ◽  
Single Wall Carbon Nanotubes ◽  
Human Dermal Fibroblasts ◽  
Intact Cells ◽  
Conducting Composite ◽  
The Matrix ◽  
Stimulation Of

Conducting composite based on biocompatible chitosan and single wall carbon nanotubes was used as a matrix for electrical stimulation of human fibroblasts. Parameters of ionic and electronic currents passing through the matrix upon applying cyclic potentials (±100 mV) were studied; the scaffold demonstrated high stability in the course of prolonged electric cycling. It was shown that preliminary electrical stimulation facilitated proliferative activity of human dermal fibroblasts in comparison to that of intact cells.

scholarly journals Induction of functional platelets from mouse and human fibroblasts by p45NF-E2/Maf

Blood ◽  
2012 ◽  
Vol 120 (18) ◽  
pp. 3812-3821 ◽  
Author(s):  
Yukako Ono ◽  
Yuhuan Wang ◽  
Hidenori Suzuki ◽  
Shinichiro Okamoto ◽  
Yasuo Ikeda ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Human Fibroblasts ◽  
Mouse Fibroblast ◽  
Dermal Fibroblasts ◽  
Induction Medium ◽  
Human Dermal Fibroblasts ◽  
3T3 Cells ◽  
Gene Expressions ◽  
Immunodeficient Mice ◽  
Adult Human

Abstract Determinant factors leading from stem cells to megakaryocytes (MKs) and subsequently platelets have yet to be identified. We now report that a combination of nuclear factor erythroid–derived 2 p45 unit (p45NF-E2), Maf G, and Maf K can convert mouse fibroblast 3T3 cells and adult human dermal fibroblasts into MKs. To screen MK-inducing factors, gene expressions were compared between 3T3 cells that do not differentiate into MKs and 3T3-L1 cells known to differentiate into MKs. 3T3 cells transfected with candidate factors were cultured in a defined MK lineage induction medium. Among the tested factors, transfection with p45NF-E2/MafG/MafK lead to the highest frequency of CD41-positive cells. Adult human dermal fibroblasts transfected with these genes were cultured in MK lineage induction medium. Cultured cells had megakaryocytic features, including surface markers, ploidy, and morphology. More than 90% of MK-sized cells expressed CD41, designated induced MK (iMK). Infusion of these iMK cells into immunodeficient mice led to a time-dependent appearance of CD41-positive, platelet-sized particles. Blood samples from iMK-infused into thrombocytopenic immunodeficient mice were perfused on a collagen-coated chip, and human CD41-positive platelets were incorporated into thrombi on the chip, demonstrating their functionality. These findings demonstrate that a combination of p45NF-E2, Maf G, and Maf K is a key determinant of both megakaryopoiesis and thrombopoiesis.

scholarly journals Senescence of Human Fibroblasts after Psoralen Photoactivation Is Mediated by ATR Kinase and Persistent DNA Damage Foci at Telomeres

2006 ◽  
Vol 17 (4) ◽  
pp. 1758-1767 ◽  
Author(s):  
Miriam Grosse Hovest ◽  
Nicole Brüggenolte ◽  
Kijawasch Shah Hosseini ◽  
Thomas Krieg ◽  
Gernot Herrmann
Keyword(s):  
Dna Damage ◽  
Human Fibroblasts ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Cross Link ◽  
Telomeric Dna ◽  
Histone H2ax ◽  
Senescent Phenotype ◽  
Atr Kinase

Cellular senescence is a phenotype that is likely linked with aging. Recent concepts view different forms of senescence as permanently maintained DNA damage responses partially characterized by the presence of senescence-associated DNA damage foci at dysfunctional telomeres. Irradiation of primary human dermal fibroblasts with the photosensitizer 8-methoxypsoralen and ultraviolet A radiation (PUVA) induces senescence. In the present study, we demonstrate that senescence after PUVA depends on DNA interstrand cross-link (ICL) formation that activates ATR kinase. ATR is necessary for the manifestation and maintenance of the senescent phenotype, because depletion of ATR expression before PUVA prevents induction of senescence, and reduction of ATR expression in PUVA-senesced fibroblasts releases cells from growth arrest. We find an ATR-dependent phosphorylation of the histone H2AX (γ-H2AX). After PUVA, ATR and γ-H2AX colocalize in multiple nuclear foci. After several days, only few predominantly telomere-localized foci persist and telomeric DNA can be coimmunoprecipitated with ATR from PUVA-senesced fibroblasts. We thus identify ATR as a novel mediator of telomere-dependent senescence in response to ICL induced by photoactivated psoralens.

scholarly journals The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.

1976 ◽  
Vol 144 (5) ◽  
pp. 1188-1203 ◽  
Author(s):  
A E Postlethwaite ◽  
R Snyderman ◽  
A H Kang
Keyword(s):  
Connective Tissue ◽  
Peripheral Blood ◽  
Human Fibroblasts ◽  
Peripheral Blood Lymphocytes ◽  
Dermal Fibroblasts ◽  
Chemotactic Factor ◽  
Human Dermal Fibroblasts ◽  
Heat Stable

A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56 degrees C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.

scholarly journals Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies

2020 ◽  
Author(s):  
Aric Anloague ◽  
Aaron Mahoney ◽  
Oladipupo Ogunbekun ◽  
William R. Thompson ◽  
Bryan Larsen ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Inflammatory Cytokines ◽  
Mechanical Stimulation ◽  
Mechanical Strain ◽  
Dermal Fibroblasts ◽  
Manual Therapies ◽  
Human Dermal Fibroblasts ◽  
Stimulation Of ◽  
Pro Inflammatory Cytokines

Abstract Objective Soft tissue manual therapies are commonly utilized by osteopathic physicians, chiropractors, physical therapists and massage therapists. These techniques are predicated on subjecting tissues to biophysical mechanical stimulation but the cellular and molecular mechanism(s) mediating these effects are poorly understood. A series of previous studies established an in vitro model system for examining mechanical stimulation of dermal fibroblasts and established that repetitive strain, intended to mimic overuse injury, induces the secretion of numerous pro-inflammatory cytokines. Moreover, mechanical strain intended to mimic soft tissue manual therapy reduces strain-induced secretion of pro-inflammatory cytokines. Here, we sought to partially confirm and extend these reports and provide independent corroboration of prior results. Results Using cultures of primary human dermal fibroblasts, we confirm mechanical forces intended to mimic repetitive motion strain increases levels of IL-6 and that mechanical strain intended to mimic therapeutic soft tissue stimulation reduces IL-6 levels. We also extend the prior work, reporting that therapy-like mechanical stimulation reduces levels of IL-8. Although there are important limitations to this experimental model, these findings provide supportive evidence that therapeutic soft tissue massage may reduce inflammation. Future work is required to address these open questions and advance the mechanistic understanding of therapeutic soft tissue stimulation.

scholarly journals Decanal Protects against UVB-Induced Photoaging in Human Dermal Fibroblasts via the cAMP Pathway

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1214 ◽  
Author(s):  
Wesuk Kang ◽  
Dabin Choi ◽  
Taesun Park
Keyword(s):  
Hyaluronic Acid ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Dermal Fibroblasts ◽  
Mitogen Activated Protein Kinase ◽  
Intracellular Camp ◽  
Activator Protein 1 ◽  
Human Dermal Fibroblasts ◽  
Beneficial Effects ◽  
The Matrix

Solar ultraviolet (UV) radiation is the primary factor of cutaneous aging, resulting in coarse wrinkles and dryness. In this study, we aimed to test whether decanal, an aromatic compound found mainly in citrus fruits, inhibits UVB-mediated photoaging in human dermal fibroblasts and to explore whether its anti-photoaging effect occurs via cyclic adenosine monophosphate (cAMP) signaling. We found that decanal promotes collagen production dose-dependently. Meanwhile, it also increased the intracellular cAMP levels and decreased the number of molecules involved in the mitogen-activated protein kinase (MAPK)/activator protein 1 (AP-1) pathway, downregulating the collagen genes and upregulating the matrix metalloproteinase (MMP) genes in UVB-exposed dermal fibroblasts. Furthermore, it enhanced hyaluronic acid levels and hyaluronic acid synthase mRNA expression. Notably, the beneficial effects of decanal were lost in the presence of a cAMP inhibitor. Our results revealed the potential of decanal for preventing photoaging and suggested that its effects are cAMP-mediated in human dermal fibroblasts.

Platelets, thrombospondin-1 and human dermal fibroblasts cooperate for stimulation of endothelial cell tubulogenesis through VEGF and PAI-1 regulation

2007 ◽  
Vol 313 (3) ◽  
pp. 486-499 ◽  
Author(s):  
Sabrina Kellouche ◽  
Samia Mourah ◽  
Arnaud Bonnefoy ◽  
Damien Schoëvaert ◽  
Marie-Pierre Podgorniak ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Thrombospondin 1 ◽  
Pai 1 ◽  
Stimulation Of
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