Year after year the number of students in higher education increases worldwide, and particularly in the virtual mode. In the face of this reality, a series of phenomena combine that have driven university institutions to reinvent themselves. The objective of this work is to analyze the influence of globalization and internationalization on the curriculum of university education, with particular emphasis on Latin American virtual education. The methodology used is the review of both literature specialized in the subject and official documents of the agencies involved. The results reveal that: 1) multilateral agencies have been made efforts to establish two-way academic partnership and cooperation agreements, on the one hand, to promote the mobility of students and teachers, as well as the realization of joint projects; on the other hand, to promote the processes of quality control and internationalization of the curriculum. 2) Regarding virtual education in the region, the need to ensure and demonstrate the quality of its programs has been set, with the Latin American and Caribbean Institute of Quality in Distance Higher Education (CALED) being one of the main references regarding guidelines and instruments for evaluation and advice to universities on quality assessment and accreditation processes. It is concluded that the internationalization of the curriculum in virtual university education in Latin America faces the challenge of taking the step towards comprehensive internationalization, that is, the one that comprehensively impacts the curriculum from a conceptual and cultural structure including interdisciplinary studies and multiculturalism.