Role of iron chelators in growth-promoting effect on mouse hybridoma cells in a chemically defined medium

1987 ◽  
Vol 23 (12) ◽  
pp. 815-820 ◽  
Author(s):  
Noritsugu Yabe ◽  
Miwa Kato ◽  
Yutaka Matsuya ◽  
Isao Yamane ◽  
Muneaki Iizuka ◽  
...  
Keyword(s):  
Defined Medium ◽  
Hybridoma Cells ◽  
Iron Chelators ◽  
Chemically Defined Medium ◽  
Promoting Effect ◽  
Growth Promoting

scholarly journals An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines

2014 ◽  
Vol 10 (10) ◽  
pp. 2713-2723 ◽  
Author(s):  
M. Burnette ◽  
T. Brito-Robinson ◽  
J. Li ◽  
J. Zartman
Keyword(s):  
Protein Interaction ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Drosophila Cell ◽  
Interaction Database ◽  
Novel Approach ◽  
Small Molecule Screen ◽  
Growth Promoting ◽  
Protein Interaction Database

We describe a novel approach to screen for growth promoting compounds and score putative targets using a drug–protein interaction database.

Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells

2007 ◽  
pp. 141-146
Author(s):  
Michael Schomberg ◽  
Christian Hakemeyer ◽  
Heino Büntemeyer ◽  
Lars Stiens ◽  
Jürgen Lehmann
Keyword(s):  
Protein Hydrolysate ◽  
Hybridoma Cells ◽  
Promoting Effect ◽  
Hydrophilic Fraction ◽  
Growth Promoting

Schistosoma mansoni: evidence for a role of serum factors in protecting artificially transformed schistosomula against antibody-mediated killing in vitro

Parasitology ◽  
1978 ◽  
Vol 77 (2) ◽  
pp. 225-233 ◽  
Author(s):  
C. A. P. Tavares ◽  
Rita C. Soares ◽  
P. M. Z. Coelho ◽  
G. Gazzinelli
Keyword(s):  
Schistosoma Mansoni ◽  
Protective Factor ◽  
Gel Filtration ◽  
Defined Medium ◽  
Rabbit Serum ◽  
Chemically Defined Medium ◽  
Lethal Effects ◽  
Serum Factors

SummaryArtificially transformed schistosomula of Schistosoma mansoni develop a consistent but small protection against the lethal effects of antibody plus complement when cultured for 24 h in a chemically defined medium. In contrast, they become rapidly resistant to antibody plus complement, when cultured in the presence of a complex medium consisting of equal parts of heat-inactivated rabbit serum and Earle's/lactalbumin or in defined medium supplemented with small amounts of heat-inactivated rabbit serum. Sephadex G-200 gel filtration revealed that the protective factor in rabbit serum is a macromolecule with a molecular weight between 7 and 19S. Parasites cultured at 10 °C or in the presence of 200 μg of puromycin show less serum-induced protection against the lethal effects of antibody plus complement than do controls.

Localization of neuraminidase-sensitive regions in the Haemophilus pleuropneumoniae Capsule by enzyme-gold affinity cytochemistry

1986 ◽  
Vol 44 ◽  
pp. 310-311
Author(s):  
W. L. Steffens ◽  
S. Kadis ◽  
I. W. Byrd
Keyword(s):  
Sialic Acid ◽  
Respiratory Disease ◽  
Virulence Factor ◽  
Colloidal Gold ◽  
Hydrolytic Enzyme ◽  
Defined Medium ◽  
Etiologic Agent ◽  
Neuraminic Acid ◽  
Chemically Defined Medium

Haemophilus pleuropneumoniae is the etiologic agent of a serious respiratory disease of swine known as porcine Haemophilus pleuropneumonia. There is some evidence suggesting the role of the capsule as a virulence factor in the pathogenesis of the organism and recent investigations have demonstrated and compared the appearance of the capsule in both virulent and avirulent strains by several mucopolysaccharide stains. Our own investigations have demonstrated variability in capsular sensitivity to the hydrolytic enzyme, neuraminidase which cleaves n-acetyl neuraminic acid (sialic acid) from mucins, including those found in several types of bacterial capsules. In an effort to localize the sites of neuraminidase sensitivity within the capsule and to possibly develop a scheme to quantitate the degree of neuraminidase sensitivity among differing serotypes and between cells of similar serotypes grown on complex and chemically defined medium, affinity cytochemistry utilizing colloidal gold-labelled neuraminidase was employed.

An Appraisal of the Role of Islamic Banking Development and Economic Growth

2020 ◽  
Vol 4 (4) ◽  
pp. 215-236
Author(s):  
Talat Hussain ◽  
Noman Arshed ◽  
Rukhsana Kalim
Keyword(s):  
Economic Growth ◽  
Resource Mobilization ◽  
Financial Inclusion ◽  
Islamic Banking ◽  
Islamic Banks ◽  
Promoting Effect ◽  
Efficient Resource ◽  
Growth Promoting ◽  
Banking Development

Literature is well-versed with the contribution of financial inclusion from the deposit and financing size and its role in economic growth. These contributions include a boost in economic transactions and efficient resource mobilization. Islamic financial system is different from conventional banking as it distributes the risk equitably and promotes fairness in dealings. It helps in the integration of business gains as a borrower of Islamic capital with the earnings of savers as depositors. This study has proposed two channels via which Islamic financial development may incur growth. First is bank financing penetration, and second is depositor financial inclusion. Based on the data of 41 full-fledged Islamic banks between 2012 and 2017, the results show that both increases in bank and depositor returns have a growth-promoting effect. This prompts the policymakers with new insights. Policymakers should increase Islamic banking penetration to different sectors and regulate for increased extraction of the depositor contribution from the banking financing activity.

Leukotriene D4-induced proliferation of glomerular epithelial cells: PKC- and Na+-H+ exchanger-mediated response

1989 ◽  
Vol 257 (2) ◽  
pp. C232-C239 ◽  
Author(s):  
L. Baud ◽  
J. Perez ◽  
G. Cherqui ◽  
E. J. Cragoe ◽  
R. Ardaillou
Keyword(s):  
Epithelial Cells ◽  
Growth Effect ◽  
Thymidine Uptake ◽  
Promoting Effect ◽  
Leukotriene D4 ◽  
Glomerular Epithelial Cells ◽  
Ic50 Values ◽  
Growth Promoting ◽  
Pkc Activation

The growth-promoting effect of leukotriene D4 (LTD4) has been observed in a variety of cells, including human glomerular epithelial cells. The purpose of this study was to determine the mechanisms underlying this process. LTD4 induction of [3H]thymidine uptake in human glomerular epithelial cells was blocked by the LTD4 receptor antagonist L648,051 when added in a 50-fold excess and by pertussis toxin. Neither drug affected basal DNA synthesis. These results suggest that the LTD4-mediated signal transduction implies activation of a GTP-binding protein that is coupled to a specific receptor. The possible role of protein kinase C (PKC) activation was also studied. In the presence of the PKC inhibitor H-7 or after downregulation of PKC levels by chronic treatment with phorbol ester, stimulation of [3H]thymidine uptake by LTD4 was greatly inhibited. Moreover, treatment of the cells by LTD4 resulted in a time-dependent increase of cytosolic PKC activity, whereas addition of phorbol 12-myristate 13-acetate reduced this activity. Therefore PKC-dependent mechanisms are likely to mediate the growth-promoting effect of LTD4. Finally, three approaches were used to determine the potential role of the Na+-H+ exchanger. First, progressive removal of extracellular Na+ using N-methyl-D-glucamine+ as a substitute inhibited LTD4-induced [3H]thymidine uptake with a 50% inhibitory concentration (IC50) of 85 mM. Second, addition of amiloride reduced the LTD4 growth effect with an IC50 of 6.5 microM, whereas three amiloride analogues exhibited lower IC50 values in accordance with their greater affinity for the Na+-H+ exchanger.(ABSTRACT TRUNCATED AT 250 WORDS)

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