Detection of Giardia lamblia and Entamoeba histolytica in Stool Samples by Two Enzyme Immunoassays

2001 ◽  
Vol 20 (6) ◽  
pp. 389-391 ◽  
Author(s):  
M. Schunk ◽  
T. Jelinek ◽  
K. Wetzel ◽  
H. D. Nothdurft
2012 ◽  
Vol 36 (1) ◽  
pp. 1-5
Author(s):  
Amjed Qays Ibrahim

In this study we collect 1520 stool samples during the period from September to December 2010 from children whom their ages between 1 month - 12 years. The results showed that the total infection of Entamoeba histolytica was 9.80% , and Giardia lamblia was 1.77%. And the male ratio that infected with Entamoeba histolytica was 9.83% , while the female ratio was 9.74%; and the male infected with Giardia lamblia was 1.51% , while the female ratio was 2.18%. The result showed that the high average of infection with Entamoeba histolytica and Giardia lamblia in age group from 1 month to 2 years. And there is no significance difference between gender and infectivity rate of Entamoeba histolytica and Giardia lamblia under P≤0.05. Also it showed that there were significant relation between Age group and infectivity rate of Entamoeba histolytica and Giardia lamblia.


2015 ◽  
Vol 43 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Hamida Khanum ◽  
Rukhshana Kadir ◽  
Tuhinur Arju ◽  
Tahmina Ahmed ◽  
Mt Tahmina Karim ◽  
...  

Tri-combo ELISA tests were carried out to determine the prevalence of protozoan parasites (Entamoeba histolytica, Giardia lamblia and Cryptosporidium spp) in 1712 stool samples of diarhoeal patients. Among the samples tested, 118 (6.89%) were found positive. The age group 6-14 years were recorded to be more susceptible (14.81%) and prevalence gradually decreased as the age group increased. Individual Antigen Detection Tests were done on the positive samples and 1 sample was noted to carry E. histolytica (0.06%), 92 (5.37%) samples with G. lamblia and 22 (1.29%) samples with Cryptosporidium spp. Maximum prevalence was recorded in summer (8.47%) and rainy seasons (8.14%) and highest infections (10.46%) were found in the month of September The prevalence of G. lamblia was higher in male (5.37%) whereas, the prevalence of Cryptosporidium spp. was higher in female (1.64%).Bangladesh J. Zool. 43(1): 1-7, 2015


Author(s):  
J. S. Danish ◽  
P. Vasanth ◽  
V. Subramanian

Introduction: Intestinal parasitic infestations are more common in developing countries and an important cause of morbidity in pediatric age group. It causes abdominal pain, diarrhoea, anemia, impaired cognitive function, growth retardation and poor academic performance among school going children. Objectives: To analyze age, gender and most common parasitic infestations among children in a tertiary care hospital in south India. Materials and Methods: Stool samples of pediatric patients in a tertiary care hospital is analysed for a period of one year for detection of prevalence of parasitic infestations. Results: In our study, 115 stool samples were analysed and prevalence of intestinal parasitic infections was found to be 7.82% (9/115).There were five different parasites encountered. The most common parasite identified was Giardia lamblia(4/9)(44.44%) followed by Entamoeba histolytica(2/9)(22.22%), Ascarislumbricoides(1/9)(11.11%), Trichuris trichiura(1/9)(11.11%), and Hymenolepis nana(1/9)(11.11%). Conclusion: Giardia lamblia and Entamoeba histolytica were found to be the most common organism in our study. Awareness about proper hygiene, sanitation and prophylactic anti-protozoal medications is important to prevent the occurrence and complications of intestinal parasitic infestations.


2015 ◽  
Vol 20 (1) ◽  
pp. 64-67
Author(s):  
Saurav K. Singh ◽  
Mahendra Maharjan

Development of reaction in leprosy patients causes severe complication in treatment. In order to analyze the association between leprosy-reaction and gastrointestinal parasitic co- infection, 100 each leprosy patients with reaction and non-reaction were identified and their stool samples were microscopically examined. The overall frequencies of intestinal parasites observed were 33 percent protozoa and 5.5 percent helminths. Entamoeba histolytica and Giardia lamblia were most prevalent parasites in both group of leprosy-reaction and non-reaction patients. Trichomonas hominis, Strongyloides stercoralis and Ancylostoma duodenales were found only in leprosy-reaction patients. There was positive co-relation between intestinal parasitic co-infection and development of reaction in leprosy patients.Journal of Institute of Science and Technology, 2015, 20(1): 64-67


2019 ◽  
Vol 9 (01) ◽  
pp. 39-45
Author(s):  
Suad Shallal Shahatha

This study was carried out to investigate the epidemiology of Giardia lamblia parasites in patients who visited some of the hospitals in Anbar province, which included (Fallujah Teaching Hospital, Ramadi Teaching Hospital, Ramadi Teaching Hospital for Women and Children and Hit Hospital) during by examining 864 stool samples in a direct examination method, The results revealed the infection rate was 41.7 % and the percentage of infection among males 47.8% is higher than that of females 35.4% with significant differences (p≤0.05). The age groups (1-9) years recorded the highest rates 55.4% and the lowest rate 13.6% in the age group (40-49) years. The highest rate of infection was 62.5% during the month of June, while the month of October was the lowest rate 5% and significant differences. The incidence rate in rural areas was 50.6% higher than in the urban areas 32.5%. The study also included the effect of Teucrium polium L. on the parasite in the culture media HSP-1, the concentrations of 0.5-3 mg / mL significantly affected Giardia, it was noted whenever the greater the concentration, the greater the effect during different treatment periods (1-4) days, as the highest concentration 3 mg/ml killed all Giardia parasites on the fourth day of treatment.


2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Vani Chandrashekar

The aim of this study was to identify common stool parasites in patients attending a tertiary care centre in South India. We evaluated 2355 stool samples and parasites were detected in 7.9% of samples. 41.1% of our patients were in the 45–58-year age group. Protozoal infections were the commonest seen in 7.8% of samples. Entamoeba histolytica was the commonest protozoa (4.6%) followed by Entamoeba coli (1.2%) and Giardia (0.8%). Entamoeba histolytica and Entamoeba coli were together seen in 0.63%, and they were the commonest organisms seen in samples with multiple-organism infection. Both were equally detected in diarrheal samples.


2017 ◽  
Vol 117 (2) ◽  
pp. 447-451 ◽  
Author(s):  
Marijo Parčina ◽  
Ingrid Reiter-Owona ◽  
Frank P. Mockenhaupt ◽  
Valerija Vojvoda ◽  
Jean Bosco Gahutu ◽  
...  

2003 ◽  
Vol 19 (4) ◽  
pp. 1205-1208 ◽  
Author(s):  
Felice Jaqueline Schnack ◽  
Lidiani de Medeiros Fontana ◽  
Paulo Roberto Barbosa ◽  
Loraine Storch Meyer da Silva ◽  
Clair Maria Martinello Baillargeon ◽  
...  

Foi investigada a presença de enteropatógenos em 94 casos de diarréia e 45 casos-controle em crianças de 0 a 5 anos de idade, atendidas no Centro de Saúde Municipal de Criciúma, Santa Catarina. Entre os parasitos isolados, o Cryptosporidium (85,1%) foi o mais freqüente, seguido pela Entamoeba histolytica (56,4%) e a Giardia lamblia (4,3%). Quatro amostras apresentaram Escherichia coli enteropatogênica (4,3%). A Samonella e a Shiguella não foram detectadas em nenhuma amostra. Somente um caso foi positivo para o rotavírus (1,1%).


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