scholarly journals Systems biology approach to identify alterations in the stem cell reservoir of subcutaneous adipose tissue in a rat model of diabetes: effects on differentiation potential and function

Diabetologia ◽  
2013 ◽  
Vol 57 (1) ◽  
pp. 246-256 ◽  
Author(s):  
Raquel Ferrer-Lorente ◽  
Maria Teresa Bejar ◽  
Monica Tous ◽  
Gemma Vilahur ◽  
Lina Badimon
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Systems Biology ◽  
Rat Model ◽  
Subcutaneous Adipose Tissue ◽  
Differentiation Potential ◽  
And Function ◽  
Subcutaneous Adipose

scholarly journals Adipose-derived mesenchymal stem cell seeded Atelocollagen scaffolds for cardiac tissue engineering

2020 ◽  
Vol 31 (10) ◽  
Author(s):  
Qiong Li ◽  
Miaomiao Li ◽  
Meng Li ◽  
Zhengyan Zhang ◽  
Han Ma ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Subcutaneous Adipose Tissue ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Good Biocompatibility ◽  
Subcutaneous Adipose

Abstract ADMSCs were isolated from subcutaneous adipose tissue, characterized and cultured in vitro. GFP-labeled ADMSCs can grow and proliferate well on the Atelocollagen scaffolds, and induced by 5-aza the cells can differentiate into cardio-like cells. 3D cultured ADMSCs on Atelocollagen scaffolds were transplanted into mice ischemia myocardium, and have good biocompatibility with host cardio tissue.

scholarly journals Characterization of human adult stem‐cell populations isolated from visceral and subcutaneous adipose tissue

2009 ◽  
Vol 23 (10) ◽  
pp. 3494-3505 ◽  
Author(s):  
Silvana Baglioni ◽  
Michela Francalanci ◽  
Roberta Squecco ◽  
Adriana Lombardi ◽  
Giulia Cantini ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Subcutaneous Adipose Tissue ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Human Adult ◽  
Stem Cell Populations ◽  
Subcutaneous Adipose

scholarly journals Regulation and Function of FTO mRNA Expression in Human Skeletal Muscle and Subcutaneous Adipose Tissue

Diabetes ◽  
2009 ◽  
Vol 58 (10) ◽  
pp. 2402-2408 ◽  
Author(s):  
L. G. Grunnet ◽  
E. Nilsson ◽  
C. Ling ◽  
T. Hansen ◽  
O. Pedersen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Mrna Expression ◽  
Subcutaneous Adipose Tissue ◽  
Human Skeletal Muscle ◽  
And Function ◽  
Subcutaneous Adipose

scholarly journals Subcutaneous Adipose Tissue–Derived Stem Cell Utility Is Independent of Anatomical Harvest Site

2015 ◽  
Vol 4 (1) ◽  
pp. 131-145 ◽  
Author(s):  
Mahmood S. Choudhery ◽  
Michael Badowski ◽  
Angela Muise ◽  
John Pierce ◽  
David T. Harris
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose ◽  
Harvest Site

scholarly journals Physiological effects of proinsulin-connecting peptide in human subcutaneous adipose tissue

2017 ◽  
Vol 104 (2) ◽  
pp. 193-205
Author(s):  
A Ghorbani ◽  
R Shafiee-Nick ◽  
SA Zojaji ◽  
MT Rajabi-Mashhadi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Physiological Effects ◽  
Media Effect ◽  
Alizarin Red ◽  
C Peptide ◽  
And Function ◽  
Subcutaneous Adipose

Recent studies suggest that proinsulin-connecting peptide (C-peptide) may exhibit characteristics of a hormone and show physiological functions in various tissues. This study was aimed to determine whether C-peptide could be involved in the regulation of lipolysis, adiponectin release, and function of mesenchymal stem cells (MSCs) in adipose tissue. Human subcutaneous adipose tissue was cultured in the presence of C-peptide. The level of lipolysis was determined by glycerol measurement in the conditioned media. Effect of C-peptide on adiponectin secretion was evaluated in differentiated adipocytes. The adipogenic and osteogenic abilities of adipose MSCs were evaluated using oil red and alizarin red staining, respectively. The tetrazolium bromide test was conducted for evaluating the effect of C-peptide on MSCs proliferation. C-peptide induced a significant decrease in basal lipolysis at concentrations of 8 and 16 nM (p < 0.05). It had no significant effects on isoproterenol-stimulated lipolysis, adiponectin secretion, and adipogenic or osteogenic differentiation of MSCs. At a concentration of 4 nM, this peptide significantly increased the proliferative capability of MSCs (p < 0.05). These results suggest that C-peptide has some physiological effects in human subcutaneous adipose tissue and contributes to the regulation of basal lipolysis and pool of MSCs.

scholarly journals Function of Chick Subcutaneous Adipose Tissue During the Embryonic and Posthatch Period

2021 ◽  
Vol 12 ◽  
Author(s):  
Haidong Zhao ◽  
Mingli Wu ◽  
Xiaoqin Tang ◽  
Qi Li ◽  
Xiaohua Yi ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Brown Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Energy Supply ◽  
Lipid Synthesis ◽  
Abdominal Adipose Tissue ◽  
Brown Adipose ◽  
And Function ◽  
Subcutaneous Adipose

Since excess abdominal fat is one of the main problems in the broiler industry for the development of modern broiler and layer industry, the importance of subcutaneous adipose tissue has been neglected. However, chick subcutaneous adipose tissue appeared earlier than abdominal adipose tissue and more than abdominal adipose tissue. Despite a wealth of data, detailed information is lacking about the development and function of chick subcutaneous adipose tissue during the embryonic and posthatch period. Therefore, the objective of the current study was to determine the developmental changes of adipocyte differentiation, lipid synthesis, lipolysis, fatty acid β-oxidation, and lipid contents from E12 to D9.5. The results showed that subcutaneous adipose tissue was another important energy supply tissue during the posthatch period. In this stage, the mitochondrial copy number and fatty acid β-oxidation level significantly increased. It revealed that chick subcutaneous adipose tissue not only has the function of energy supply by lipidolysis but also performs the same function as brown adipose tissue to some extent, despite that the brown adipose tissue does not exist in birds. In addition, this finding improved the theory of energy supply in the embryonic and posthatch period and might provide theoretical basis on physiological characteristics of lipid metabolism in chicks.

scholarly journals A novel genetically-obese rat model with elevated 11beta-hydroxysteroid dehydrogenase type 1 activity in subcutaneous adipose tissue

2010 ◽  
Vol 9 (1) ◽  
pp. 132 ◽  
Author(s):  
Sakamuri SS Vara Prasad ◽  
Anamthathmakula Prashanth ◽  
Chodavarapu Pavan Kumar ◽  
Sirisha J Reddy ◽  
Nappan V Giridharan ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rat Model ◽  
Subcutaneous Adipose Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Subcutaneous Adipose

scholarly journals Characterization of adipose tissue macrophages and adipose-derived stem cells in critical wounds

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2824 ◽  
Author(s):  
Bong-Sung Kim ◽  
Pathricia V. Tilstam ◽  
Katrin Springenberg-Jung ◽  
Arne Hendrick Boecker ◽  
Corinna Schmitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Subcutaneous Adipose Tissue ◽  
Stem Cell Markers ◽  
Adipose Derived Stem Cells ◽  
Cell Markers ◽  
Adipose Tissue Macrophages ◽  
Subcutaneous Adipose

Background Subcutaneous adipose tissue is a rich source of adipose tissue macrophages and adipose-derived stem cells which both play a key role in wound repair. While macrophages can be divided into the classically-activated M1 and the alternatively-activated M2 phenotype, ASCs are characterized by the expression of specific stem cell markers. Methods In the present study, we have investigated the expression of common macrophage polarization and stem cell markers in acutely inflamed adipose tissue. Subcutaneous adipose tissue adjacent to acutely inflamed wounds of 20 patients and 20 healthy subjects were harvested and underwent qPCR and flow cytometry analysis. Results Expression levels of the M1-specific markers CD80, iNOS, and IL-1b were significantly elevated in inflammatory adipose tissue when compared to healthy adipose tissue, whereas the M2-specific markers CD163 and TGF-β were decreased. By flow cytometry, a significant shift of adipose tissue macrophage populations towards the M1 phenotype was confirmed. Furthermore, a decrease in the mesenchymal stem cell markers CD29, CD34, and CD105 was observed whereas CD73 and CD90 remained unchanged. Discussion This is the first report describing the predominance of M1 adipose tissue macrophages and the reduction of stem cell marker expression in acutely inflamed, non-healing wounds.

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