scholarly journals Comprehensive evaluation and analysis of the salinity stress response mechanisms based on transcriptome and metabolome of Staphylococcus aureus

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Ying Feng ◽  
Dizhou Gu ◽  
Ziyan Wang ◽  
Chenyang Lu ◽  
Jingfeng Fan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stress Response ◽  
Salinity Stress ◽  
Comprehensive Evaluation

scholarly journals Comprehensive Evaluation and Analysis of the Salinity Stress Response Mechanisms Based on Transcriptome and Metabolome of Staphylococcus Aureus

2021 ◽  
Author(s):  
Ying Feng ◽  
Dizhou Gu ◽  
Ziyan Wang ◽  
Chenyang Lu ◽  
Jingfeng Fan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Salinity Stress ◽  
Target Genes ◽  
Comprehensive Evaluation ◽  
Inositol Phosphate ◽  
Treatment Strategies ◽  
Tca Cycle ◽  
Metabolism Pathway ◽  
Inositol Phosphate Metabolism ◽  
Wide Range

Abstract Staphylococcus aureus possesses an extraordinary ability to deal with a wide range of osmotic pressure. To performed transcriptomic and metabolomic analyses on the potential mechanism of gradient salinity stress adaptation in S. aureus ZS01. The results revealed that CPS biosynthetic protein genes were candidate target genes for directly regulating the phenotypic changes of biofilm. Inositol phosphate metabolism was downregulated to reduce the conversion of functional molecules. Gluconeogenesis pathway was downregulated to reduce the production of endogenous glucose. Pyruvate metabolism pathway was upregulated to promote the accumulation of succinate. TCA cycle metabolism pathway was downregulated to reduce unnecessary energy loss. These self-protection mechanisms can protect cells from hypertonic environments, and help them focus on survival. In addition, we identified 10 hub genes. The findings will aid in the prevention and treatment strategies of S. aureus infections.

scholarly journals Genome-Wide Identification of Resveratrol Intrinsic Resistance Determinants in Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 82
Author(s):  
Liping Liu ◽  
Hanne Ingmer ◽  
Martin Vestergaard
Keyword(s):  
Staphylococcus Aureus ◽  
Stress Response ◽  
Inhibitory Activity ◽  
Bacterial Species ◽  
Dna Integrity ◽  
Intrinsic Resistance ◽  
Potential Health ◽  
Cellular Effects ◽  
Development Of Resistance ◽  
Wide Range

Resveratrol has been extensively studied due to its potential health benefits in multiple diseases, for example, cancer, obesity and cardiovascular diseases. Besides these properties, resveratrol displays inhibitory activity against a wide range of bacterial species; however, the cellular effects of resveratrol in bacteria remain incompletely understood, especially in the human pathogen, Staphylococcus aureus. In this study, we aimed to identify intrinsic resistance genes that aid S. aureus in tolerating the activity of resveratrol. We screened the Nebraska Transposon Mutant Library, consisting of 1920 mutants with inactivation of non-essential genes in S. aureus JE2, for increased susceptibly to resveratrol. On agar plates containing 0.5× the minimum inhibitory concentration (MIC), 17 transposon mutants failed to grow. Of these, four mutants showed a two-fold reduction in MIC, being the clpP protease mutant and three mutants with deficiencies in the electron transport chain (menD, hemB, aroC). The remaining 13 mutants did not show a reduction in MIC, but were confirmed by spot-assays to have increased susceptibility to resveratrol. Several genes were associated with DNA damage repair (recJ, xerC and xseA). Treatment of S. aureus JE2 with sub-inhibitory concentrations of resveratrol did not affect the expression of recJ, xerC and xseA, but increased expression of the SOS–stress response genes lexA and recA, suggesting that resveratrol interferes with DNA integrity in S. aureus. Expression of error-prone DNA polymerases are part of the SOS–stress response and we could show that sub-inhibitory concentrations of resveratrol increased overall mutation frequency as measured by formation of rifampicin resistant mutants. Our data show that DNA repair systems are important determinants aiding S. aureus to overcome the inhibitory activity of resveratrol. Activation of the SOS response by resveratrol could potentially facilitate the development of resistance towards conventional antibiotics in S. aureus.

Genomewide discovery of DNA polymorphisms in rice cultivars with contrasting drought and salinity stress response and their functional relevance

2013 ◽  
Vol 12 (2) ◽  
pp. 253-264 ◽  
Author(s):  
Mukesh Jain ◽  
Kanhu Charan Moharana ◽  
Rama Shankar ◽  
Romika Kumari ◽  
Rohini Garg
Keyword(s):  
Stress Response ◽  
Salinity Stress ◽  
Dna Polymorphisms ◽  
Rice Cultivars ◽  
Functional Relevance

Suppression of Thiol-Dependent Antioxidant System and Stress Response in Methicillin-Resistant Staphylococcus aureus by Docosanol: Explication Through Proteome Investigation

2022 ◽  
Author(s):  
Selvaraj Alagu Lakshmi ◽  
Krishnan Ganesh Prasath ◽  
Kannapiran Tamilmuhilan ◽  
Adimoolam Srivathsan ◽  
Raja Mohamed Beema Shafreen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stress Response ◽  
Antioxidant System ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

scholarly journals MicroRNA396-mediated alteration in plant development and salinity stress response in creeping bentgrass

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Shuangrong Yuan ◽  
Junming Zhao ◽  
Zhigang Li ◽  
Qian Hu ◽  
Ning Yuan ◽  
...  
Keyword(s):  
Stress Response ◽  
Salinity Stress ◽  
Plant Development ◽  
Creeping Bentgrass

scholarly journals Investigation of the Staphylococcus aureus GraSR Regulon Reveals Novel Links to Virulence, Stress Response and Cell Wall Signal Transduction Pathways

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e21323 ◽  
Author(s):  
Mélanie Falord ◽  
Ulrike Mäder ◽  
Aurélia Hiron ◽  
Michel Débarbouillé ◽  
Tarek Msadek
Keyword(s):  
Signal Transduction ◽  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Stress Response ◽  
Signal Transduction Pathways ◽  
Wall Signal

scholarly journals Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations

Virulence ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 1013-1025 ◽  
Author(s):  
Emily Schifano ◽  
Graziella Ficociello ◽  
Simone Vespa ◽  
Salil Ghosh ◽  
John F Cipollo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Aureus Infection
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