Occurrence, molecular characterization and antimicrobial-resistance pattern of Staphylococcus species isolates from buck semen

2022 ◽  
Vol 204 (2) ◽  
Author(s):  
Gururaj Kumaresan ◽  
Chetna Gangwar ◽  
Anil Kumar Mishra ◽  
Ashok Kumar ◽  
Suresh Dinkar Kharche ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Resistance Pattern

Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

2021 ◽  
Author(s):  
Johana Elizabeth Dominguez ◽  
María Rosa Viñas ◽  
Mariana Herrera ◽  
Mirian Moroni ◽  
Gabriel Omar Gutkind ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Salmonella Heidelberg

scholarly journals Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon

2021 ◽  
Vol 9 (6) ◽  
pp. 1148
Author(s):  
Zahie Abboud ◽  
Lucia Galuppo ◽  
Marco Tolone ◽  
Maria Vitale ◽  
Roberto Puleio ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility ◽  
Meca Gene ◽  
Toxin Gene ◽  
Pcr Screening ◽  
E Coli ◽  
Presenting Symptoms ◽  
Flight Mass Spectrometry ◽  
Streptococcus Uberis

Mastitis is an infectious disease encountered in dairy animals worldwide that is currently a growing concern in Lebanon. This study aimed at investigating the etiology of the main mastitis-causing pathogens in Northern Lebanon, determining their antimicrobial susceptibility profiles, and identifying their antimicrobial resistance (AMR) genes. A total of 101 quarter milk samples were collected from 77 cows and 11 goats presenting symptoms of mastitis on 45 dairy farms. Bacterial identification was carried out through matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Antimicrobial susceptibility was tested by disc diffusion and broth microdilution methods. Molecular characterization included polymerase chain reaction (PCR) screening for genes encoding extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC among Enterobacterales isolates, and virulence factors among Staphylococcus isolates. Escherichia coli isolates were subjected to phylogenetic typing by a quadruplex PCR method. The most frequently identified species were Streptococcus uberis (19.2%), Streptococcus agalactiae (15.1%), E. coli (12.3%), and Staphylococcus aureus (10.96%). Gram-positive bacteria were resistant to macrolides and tetracycline, whereas gram-negative bacteria displayed resistance to ampicillin and tetracycline. Two ESBL genes, blaTEM (83.3%) and blaOXA (16.7%), and one AmpC beta-lactamase gene, blaCMY-II (16.7%), were detected among six E. coli isolates, which mainly belonged to phylogenetic group B1. Among Staphylococcus spp., the mecA gene was present in three isolates. Furthermore, four isolates contained at least one toxin gene, and all S. aureus isolates carried the ica operon. These findings revealed the alarming risk of AMR in the Lebanese dairy chain and the importance of monitoring antimicrobial usage.

Molecular Characterization and Antibiotic Resistance Pattern of Isolated Acinetobacter baumannii in Iran

Gene Reports ◽  
2021 ◽  
pp. 101195
Author(s):  
Hossein Masoumi-Asl ◽  
Fatemah Sadeghpour Heravi ◽  
Ali Badamchi ◽  
Khadijeh Khanaliha ◽  
Marziye Farsimadan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Resistance Pattern ◽  
Antibiotic Resistance Pattern

Comparison of Prevalence, Antimicrobial Resistance, and Occurrence of Multidrug-Resistant Salmonella in Antimicrobial-Free and Conventional Pig Production

2006 ◽  
Vol 69 (4) ◽  
pp. 743-748 ◽  
Author(s):  
WONDWOSSEN A. GEBREYES ◽  
SIDDHARTHA THAKUR ◽  
W. E. MORGAN MORROW
Keyword(s):  
Antimicrobial Resistance ◽  
Production Systems ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Swine Production ◽  
Significant Difference ◽  
Conventional Farms ◽  
Resistance Patterns ◽  
Conventional Systems ◽  
On Farm

Conventional swine production evolved to routinely use antimicrobials, and common occurrence of antimicrobial-resistant Salmonella has been reported. There is a paucity of information on the antimicrobial resistance of Salmonella in swine production in the absence of antimicrobial selective pressure. Therefore, we compared the prevalence and antimicrobial resistance of Salmonella isolated from antimicrobial-free and conventional production systems. A total of 889 pigs and 743 carcasses were sampled in the study. Salmonella prevalence was significantly higher among the antimicrobial-free systems (15.2%) than the conventional systems (4.2%) (odds ratio [OR] = 4.23; P < 0.05). Antimicrobial resistance was detected against 10 of the 12 antimicrobials tested. The highest frequency of resistance was found against tetracycline (80%), followed by streptomycin (43.4%) and sulfamethoxazole (36%). Frequency of resistance to most classes of antimicrobials (except tetracycline) was significantly higher among conventional farms than antimicrobial-free farms, with ORs ranging from 2.84 for chloramphenicol to 23.22 for kanamycin at the on-farm level. A total of 28 antimicrobial resistance patterns were detected. A resistance pattern with streptomycin, sulfamethoxazole, and tetracycline (n = 130) was the most common multidrug resistance pattern. There was no significant difference in the proportion of isolates with this pattern between the conventional (19.5%) and the antimicrobial-free systems (18%) (OR = 1.8; P > 0.05). A pentaresistance pattern with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline was strongly associated with antimicrobial-free groups (OR = 5.4; P = 0.01). While showing the higher likelihood of finding antimicrobial resistance among conventional herds, this study also implies that specific multidrug-resistant strains may occur on antimicrobial-free farms. A longitudinal study with a representative sample size is needed to reach more conclusive results of the associations detected in this study.

Sign in / Sign up

Export Citation Format

Share Document