Antimicrobial Resistance analysis of Pathogenic Bacteria Isolated from Freshwater Nile Tilapia (Oreochromis niloticus) Cultured in Kerala, India

2020 ◽  
Vol 77 (11) ◽  
pp. 3278-3287
Author(s):  
P. G. Preena ◽  
Arathi Dharmaratnam ◽  
T. Raja Swaminathan
Keyword(s):  
Antimicrobial Resistance ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Resistance Analysis ◽  
Nile Tilapia Oreochromis Niloticus

Efficacy of Bacillus spp. isolated from Nile tilapia Oreochromis niloticus Linn. on its growth and immunity, and control of pathogenic bacteria

2020 ◽  
Vol 86 (2) ◽  
pp. 353-365 ◽  
Author(s):  
Nutnicha Sookchaiyaporn ◽  
Prapansak Srisapoome ◽  
Sasimanas Unajak ◽  
Nontawith Areechon
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Bacillus Spp ◽  
And Control

Prevalence and Antimicrobial Resistance of Salmonella spp. in Aquacultured Nile Tilapia (Oreochromis niloticus) Commercialized in Federal District, Brazil

2021 ◽  
Author(s):  
Ana Carolina Almeida de Oliveira Ferreira ◽  
Sabrina Lunara Santos Pavelquesi ◽  
Erika da Silva Monteiro ◽  
Letícia Fernandes Silva Rodrigues ◽  
Calliandra Maria de Souza Silva ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Federal District ◽  
Salmonella Spp ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Investigating the Effect of an Oxytetracycline Treatment on the Gut Microbiome and Antimicrobial Resistance Gene Dynamics in Nile Tilapia (Oreochromis niloticus)

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1213
Author(s):  
Christopher J. Payne ◽  
James F. Turnbull ◽  
Simon MacKenzie ◽  
Margaret Crumlish
Keyword(s):  
Antimicrobial Resistance ◽  
Antibiotic Treatment ◽  
Resistance Genes ◽  
Gut Microbiome ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Antimicrobial Resistance Genes ◽  
Antimicrobial Resistance Gene ◽  
The Impact

Antibiotics play a vital role in aquaculture where they are commonly used to treat bacterial diseases. However, the impact of antibiotic treatment on the gut microbiome and the development of antimicrobial resistance in Nile tilapia (Oreochromis niloticus) over time remains to be fully understood. In this study, fish were fed a single treatment of oxytetracycline (100 mg/kg/day) for eight days, followed by a 14-day withdrawal period. Changes in the distal gut microbiome were measured using 16S rRNA sequencing. In addition, the abundance of antimicrobial resistance genes was quantified using real-time qPCR methods. Overall, the gut microbiome community diversity and structure of Nile tilapia was resilient to oxytetracycline treatment. However, antibiotic treatment was associated with an enrichment in Plesiomonas, accompanied by a decline in other bacteria taxa. Oxytetracycline treatment increased the proportion of tetA in the distal gut of fish and tank biofilms of the treated group. Furthermore, the abundance of tetA along with other tetracycline resistance genes was strongly correlated with a number of microbiome members, including Plesiomonas. The findings from this study demonstrate that antibiotic treatment can exert selective pressures on the gut microbiome of fish in favour of resistant populations, which may have long-term impacts on fish health.

scholarly journals Identification of Pathogenic Bacteria from Diseased Nile Tilapia Oreochromis niloticus with their Sensitivity to Antibiotics

2020 ◽  
Vol 9 (3) ◽  
pp. 1716-1738
Author(s):  
Aishi Hamom ◽  
M. M. Mahbub Alam ◽  
Mohammed Mahbub Iqbal ◽  
Sarker Mohammed q Ibrahim Khalil ◽  
Moslima Parven ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Infection prevention of Aeromonas hydrophila bacteria in Nile tilapia (Oreochromis niloticus) by providing ethyl acetate extract of temu ireng (Curcuma aeruginosa) rhizome

2013 ◽  
Vol 11 (2) ◽  
pp. 31-35
Author(s):  
DINA SELVIA SARI ◽  
ARTINI PANGASTUTI ◽  
ELISA HERAWATI
Keyword(s):  
Ethyl Acetate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Infection Prevention ◽  
Ethyl Acetate Extract ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
The One

Sari DS, Pangastuti A, Elisa Herawati E. 2013. Infection prevention of Aeromonas hydrophila bacteria in Nile tilapia (Oreochromis niloticus) by providing ethyl acetate extract of temu ireng (Curcuma aeruginosa) rhizome. Biofarmasi 11: 31-35. In tilapia aquaculture, some diseases can disturb the growth and production of fish. Aeromonas hydrophila is one of the pathogenic bacteria that can cause a disease in tilapia. Aeromonas hydrophila uses a quorum sensing system and the virulence of organisms as a controller to other organisms. The one of infection prevention effort of A. hydrophila that efficient enough is to use a compound of natural ingredients, i.e. Curcuma aeruginosa rhizome. The purpose of this study was to determine the optimal concentration of ethyl acetate extract of Curcuma aeruginosa rhizome that needed to prevent the infection of A. hydrophila bacterial in tilapia. The method used in this study was an immersion method. Tilapia was soaked in water mixed with A. hydrophila and the ethyl acetate extract of C. aeruginosa rhizome with the concentrations of 0 mL/L, 10 mL/L, 20 mL/L, 30 mL/L, 40 mL/L, 50 mL/L and control for 90 minutes. At the end of the study, it was observed for the fish behavior after immersion, the reaction of fish, the type and morphology of fish, and the number of bacteria in the water conservancy. The results showed that the A. hydrophila infection could be prevented by using the ethyl acetate extract of the C. aeruginosa rhizome with the concentration of 40 mL/L. During immersion, tilapia was get an experience stress, often to the surface of water, and then quietly at the bottom of aquarium. The response to eat of tilapia decreased by 50% after soaking, but after 2-3 days of immersion time, the fish feeding was normally again.

scholarly journals Molecular Characterization and Defense Functions of the Nile Tilapia (Oreochromis niloticus) DnaJ B9b and DnaJ C3a Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1509
Author(s):  
Prapansak Srisapoome ◽  
Kubpaphas Thummabancha ◽  
Ratree Wongpanya
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Head Kidney ◽  
High Temperature Stress ◽  
Stress Conditions ◽  
Flavobacterium Columnare ◽  
Nile Tilapia Oreochromis Niloticus

DnaJ proteins or heat shock protein 40s (HSP40s) form one of the largest heat shock protein families. In this study, 2 cDNAs encoding Nile tilapia (Oreochromis niloticus) DnaJ proteins (On-DnaJ B9b and On-DnaJ C3a) were successfully cloned and characterized. The structures and organizations of these two genes are first reported in the present study. On-DnaJ B9b is approximately 2.1 kb long and contains 2 exons and 1 intron, while On-DnaJ C3a is approximately 12 kb long and contains 12 exons and 11 introns. Under normal conditions, On-DnaJ B9b mRNA is highly expressed in gonad and trunk kidney tissues, while On-DnaJ C3a transcripts are abundantly expressed in gills, intestine, liver, and trunk kidney tissues. Following pathogenic infections, the expression of both genes is induced in the liver, spleen and head kidney tissues of Nile tilapia that were infected with two virulent pathogenic bacteria, Streptococcus agalactiae and Flavobacterium columnare. Silencing of these two genes was first carried out, and the results clearly indicated their crucial roles under both heat and bacterial stress conditions. The fundamental knowledge obtained from this study indicates the characteristic basic biofunctions of heat shock proteins in the regulation of intracellular proteins during infection, which involve preventing protein aggregation, promoting protein refolding, and activating unfolded protein degradation.

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