Efficacy of Bacillus spp. isolated from Nile tilapia Oreochromis niloticus Linn. on its growth and immunity, and control of pathogenic bacteria

2020 ◽  
Vol 86 (2) ◽  
pp. 353-365 ◽  
Author(s):  
Nutnicha Sookchaiyaporn ◽  
Prapansak Srisapoome ◽  
Sasimanas Unajak ◽  
Nontawith Areechon
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Bacillus Spp ◽  
And Control

scholarly journals Identification of Pathogenic Bacteria from Diseased Nile Tilapia Oreochromis niloticus with their Sensitivity to Antibiotics

2020 ◽  
Vol 9 (3) ◽  
pp. 1716-1738
Author(s):  
Aishi Hamom ◽  
M. M. Mahbub Alam ◽  
Mohammed Mahbub Iqbal ◽  
Sarker Mohammed q Ibrahim Khalil ◽  
Moslima Parven ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Management Strategies for Nile Tilapia (Oreochromis niloticus) Hatchery in the Face of Climate Change Induced Rising Temperature

2021 ◽  
Vol 21 (2) ◽  
pp. 55-62
Author(s):  
S. M. Ashiqul Alam ◽  
Md. Shirajul Islam Sarkar ◽  
Md. Mahbubul Alam Miah ◽  
Harunur Rashid
Keyword(s):  
Climate Change ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Egg Production ◽  
Management Strategies ◽  
Commercial Fish ◽  
Nile Tilapia Oreochromis Niloticus ◽  
The Face ◽  
Increasing Temperature ◽  
And Control

In the quest for appropriate management strategies for less egg production in Nile tilapia (Oreochromis niloticus) due to climate change induced increasing temperature, five treatments (T): shade with cloth over brood hapa (T1), increase in pond depth (T2), aeration (T3), combination of above three interventions (T4) and control (no intervention) (T5) were investigated in a commercial hatchery in Mymensingh, Bangladesh during April to September. Mean egg production in T1, T2, T3, T4 and T5 was 20488, 15369, 3596, 21021 and 3979 eggs/hapa, respectively. T1 was the best strategy considered due to efficiency and simplicity. In May T1, T2, T3, T4 and T5 produced highest 30859, 36119, 8997, 45876 and 5506 eggs/hapa at 29.69°C, 30.12°C, 29.96°C, 29.61°C, and 31.26°C temperature, respectively. The most suitable water temperature for highest egg production (20365 eggs/hapa) was 29-31°C. Egg production above 32°C was found to be very low (179 eggs/hapa). Suitable ranges of dissolved oxygen, pH, alkalinity, ammonia and turbidity for egg production were found to be 4.5-6.0 mg/L, 8.0-8.8, 105-150 mg/L, 0-0.5 mg/L and 15-35 cm, respectively. In high temperature months commercial fish hatcheries should use shed with cloth over brood hapa to produce higher amount eggs.

scholarly journals Infection prevention of Aeromonas hydrophila bacteria in Nile tilapia (Oreochromis niloticus) by providing ethyl acetate extract of temu ireng (Curcuma aeruginosa) rhizome

2013 ◽  
Vol 11 (2) ◽  
pp. 31-35
Author(s):  
DINA SELVIA SARI ◽  
ARTINI PANGASTUTI ◽  
ELISA HERAWATI
Keyword(s):  
Ethyl Acetate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Infection Prevention ◽  
Ethyl Acetate Extract ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
The One

Sari DS, Pangastuti A, Elisa Herawati E. 2013. Infection prevention of Aeromonas hydrophila bacteria in Nile tilapia (Oreochromis niloticus) by providing ethyl acetate extract of temu ireng (Curcuma aeruginosa) rhizome. Biofarmasi 11: 31-35. In tilapia aquaculture, some diseases can disturb the growth and production of fish. Aeromonas hydrophila is one of the pathogenic bacteria that can cause a disease in tilapia. Aeromonas hydrophila uses a quorum sensing system and the virulence of organisms as a controller to other organisms. The one of infection prevention effort of A. hydrophila that efficient enough is to use a compound of natural ingredients, i.e. Curcuma aeruginosa rhizome. The purpose of this study was to determine the optimal concentration of ethyl acetate extract of Curcuma aeruginosa rhizome that needed to prevent the infection of A. hydrophila bacterial in tilapia. The method used in this study was an immersion method. Tilapia was soaked in water mixed with A. hydrophila and the ethyl acetate extract of C. aeruginosa rhizome with the concentrations of 0 mL/L, 10 mL/L, 20 mL/L, 30 mL/L, 40 mL/L, 50 mL/L and control for 90 minutes. At the end of the study, it was observed for the fish behavior after immersion, the reaction of fish, the type and morphology of fish, and the number of bacteria in the water conservancy. The results showed that the A. hydrophila infection could be prevented by using the ethyl acetate extract of the C. aeruginosa rhizome with the concentration of 40 mL/L. During immersion, tilapia was get an experience stress, often to the surface of water, and then quietly at the bottom of aquarium. The response to eat of tilapia decreased by 50% after soaking, but after 2-3 days of immersion time, the fish feeding was normally again.

scholarly journals Molecular Characterization and Defense Functions of the Nile Tilapia (Oreochromis niloticus) DnaJ B9b and DnaJ C3a Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1509
Author(s):  
Prapansak Srisapoome ◽  
Kubpaphas Thummabancha ◽  
Ratree Wongpanya
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Head Kidney ◽  
High Temperature Stress ◽  
Stress Conditions ◽  
Flavobacterium Columnare ◽  
Nile Tilapia Oreochromis Niloticus

DnaJ proteins or heat shock protein 40s (HSP40s) form one of the largest heat shock protein families. In this study, 2 cDNAs encoding Nile tilapia (Oreochromis niloticus) DnaJ proteins (On-DnaJ B9b and On-DnaJ C3a) were successfully cloned and characterized. The structures and organizations of these two genes are first reported in the present study. On-DnaJ B9b is approximately 2.1 kb long and contains 2 exons and 1 intron, while On-DnaJ C3a is approximately 12 kb long and contains 12 exons and 11 introns. Under normal conditions, On-DnaJ B9b mRNA is highly expressed in gonad and trunk kidney tissues, while On-DnaJ C3a transcripts are abundantly expressed in gills, intestine, liver, and trunk kidney tissues. Following pathogenic infections, the expression of both genes is induced in the liver, spleen and head kidney tissues of Nile tilapia that were infected with two virulent pathogenic bacteria, Streptococcus agalactiae and Flavobacterium columnare. Silencing of these two genes was first carried out, and the results clearly indicated their crucial roles under both heat and bacterial stress conditions. The fundamental knowledge obtained from this study indicates the characteristic basic biofunctions of heat shock proteins in the regulation of intracellular proteins during infection, which involve preventing protein aggregation, promoting protein refolding, and activating unfolded protein degradation.

scholarly journals PENINGKATAN KEKEBALAN SPESIFIK ANTI STREPTOCOCCUS PADA BUDI DAYA IKAN NILA

2016 ◽  
Vol 2 (1) ◽  
pp. 87
Author(s):  
Hambali Supriyadi ◽  
Desy Sugiani ◽  
Uni Purwaningsih
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Field Study ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Vaccine Delivery ◽  
Streptococcus Iniae ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Net Cage ◽  
And Control

Uji lapang vaksin Streptococcus telah dilakukan dengan tujuan untuk mengetahui efektivitas vaksin dan respon kebal pada ikan nila (Oreochromis niloticus) terhadap rangsangan yang diberikan. Penelitian dilakukan pada keramba jaring apung (KJA). Vaksin yang digunakan adalah vaksin S1N8 dan GM2.4 berupa vaksin yang diinaktivasi dengan formalin 0,3%. Aplikasi vaksin dilakukan secara bertahap yaitu vaksin awal (priming) diberikan melalui rendaman, sedangkan vaksin ulang diberikan melalui suntikan. Dosis vaksin awal yaitu 10 mL vaksin/100 L air untuk 1.000 ekor ikan direndam selama 15 menit, sedangkan booster diberikan melalui suntikan 0,2 mL/ ekor ikan. Hasil menunjukkan bahwa kombinasi pemberian vaksin priming melalui rendaman dan booster dengan suntikan untuk vaksin S1N8 menghasilkan sintasan paling tinggi (70,3%—72,5%), apabila dibandingkan dengan vaksin GM2.4 (59,3%— 62,5%) dan kontrol (35,5%—42,0%).Field study of vaccines S1N8 and GM2.4 with the aims to evaluate the effectiveness of vaccine and the immune response of nile tilapia ( Oreochromis niloticus ) against the vaccines. The research have been conducted in floating net cage. Vaccine tested were produced from Streptococcus iniae isolates S1N8 and GM2.4 which was prepared by formalin killed of 0.3%(v/v). Vaccine delivery were given in two steps i.e. priming with immersion, and booster through injection. The dose of vaccine for priming was 10 mL of vaccine/100 L water immersed for 1,000 fish for 15 minutes. Booster were delivered by injection as much as 0.2 mL/fish. The results indicated that combination of vaccine delivering of immersion (priming) and injection (booster) especially for S1N8 vaccine were the highest percent of survival rate (70.3%—72.5%) as compared with GM2.4 vaccine (59.3%—62.5%) and control (35.5%—42.0%).

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