Characterization of potato (Solanum tuberosum) and tomato (Solanum lycopersicum) protein phosphatases type 2A catalytic subunits and their involvement in stress responses

Planta ◽  
2009 ◽  
Vol 230 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Silvia Marina País ◽  
Marina Alejandra González ◽  
María Teresa Téllez-Iñón ◽  
Daniela Andrea Capiati
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Lycopersicum ◽  
Stress Responses ◽  
Protein Phosphatases ◽  
Catalytic Subunits

scholarly journals Caracterización de razas fisiológicas de Phytophthora infestans (Mont.) de Bary en lulo (Solanum quitoense Lam.)

2012 ◽  
Vol 13 (1) ◽  
pp. 13 ◽  
Author(s):  
José Luis Zapata P. ◽  
Jorge A. Bernal E.
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Solanum Lycopersicum ◽  
Virulence Factors ◽  
Virulence Factor ◽  
Cyphomandra Betacea ◽  
Physiological Races ◽  
Solanum Muricatum ◽  
Tree Tomato

<p>El tizón del lulo es uno de los problemas más importantes de este cultivo en Colombia, presente en todos los departamentos luleros del país, con una incidencia del 100%. El desconocimiento en la complejidad de las razas fisiológicas de<em>Phytophthora infestans </em>en cultivos de lulo (<em>Solanum quitoense</em>) se evaluaron colectas provenientes de este cultivo<em>, </em>papa (<em>Solanum tuberosum </em>sub especie <em>andigena</em>), tomate de árbol (<em>Cyphomandra betacea</em>), tomate de mesa (<em>Solanum lycopersicum</em>) y pepino de agua o pepino dulce (<em>Solanum muricatum</em>), obtenidas en varios departamentos de Colombia productores de lulo. Los resultados obtenidos sobre la virulencia del patógeno realizada sobre 11 diferenciales de papa (junto con la variedad Tuquerreña), indican para el lulo, la presencia de 15 razas fisiológicas, según nomenclatura octal siendo la más frecuente la 4211 con 23,25% de frecuencia y los factores de virulencia 0, 4, 8 y 11, colectada en los municipios de Urrao y Rionegro seguida de la 2211, 0213, 0231 y 1112 con 4 factores de virulencia y una frecuencia de 2,32% cada una, colectadas en los municipios de Oporapa, Jardín y Santa Rosa de Cabal, respectivamente. Igualmente, se encontró la raza 3223 con los factores de virulencia 1, 2, 4, 7, 10, y 11 procedente de papa, la raza 4421 con los factores 0, 3, 7, 10 y 11 colectada en tomate de mesa y la 4.000 con el factor de virulencia 0, procedente de pepino, lo cual indica que el patógeno procedente de papa, es el más complejo. </p><p> </p><p><strong>Characterization of physiological races of <em>Phytophthora infestans </em>(Mont.) de Bary in lulo (<em>Solanum quitoense</em>Lam.)</strong></p><p> Lulo blight is one of the most important problems of this crop in Colombia, present in all departments of the country that grow it, with an incidence of 100%. Due to the ignorance of the complexity of physiological races of <em>Phytophthora infestans </em>in cultivated lulo <em>(Solanum quitoense</em>), collections were evaluated from potato (<em>Solanum tuberosum </em>sub species <em>andigena</em>), tree tomato (<em>Cyphomandra betacea</em>) , tomato (<em>Solanum lycopersicum</em>) and water cucumber or sweet cucumber (<em>Solanum muricatum</em>) obtained in various lulo producing departments of Colombia. The results obtained on the virulence of the pathogen on 11 potato differentials (along with the variety Tuquerreña) indicated that for lulo there are 15 physiological races with, according to octal nomenclature, 4211 being the most frequent with 23.25% frequency and virulence factors of 0, 4, 8 and 11, collected in the municipalities of Rionegro and Urrao, followed by 2211, 0213, 0231 and 1112 with four virulence factors and a frequency of 2.32% each, collected in the municipalities Oporapa, Jardín, Jardín and Santa Rosa de Cabal, respectively. Similarly, we found 3223 with virulence factors 1, 2, 4, 7, 10, and 11 in potato, 4421 with factors 0, 3, 7, 10 and 11 collected from the tomato and 4,000 with virulence factor 0 in cucumber; indicating that the pathogen from potato is the most complex. </p>

scholarly journals Identification and Characterization of LRR-RLK Family Genes in Potato Reveal Their Involvement in Peptide Signaling of Cell Fate Decisions and Biotic/Abiotic Stress Responses

Cells ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 120 ◽  
Author(s):  
Xiaoxu Li ◽  
Ahmad Salman ◽  
Cun Guo ◽  
Jing Yu ◽  
Songxiao Cao ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Cell Fate ◽  
Stress Responses ◽  
Feedback Loop ◽  
Peptide Recognition ◽  
Cell Fate Decisions ◽  
Duplication Events ◽  
Peptide Signals ◽  
Identification And Characterization

Leucine-rich repeat receptor-like kinases (LRR-RLKs) represent the largest subfamily of receptor-like kinases (RLKs) and play important roles in regulating growth, development, and stress responses in plants. In this study, 246 LRR-RLK genes were identified in the potato (Solanum tuberosum) genome, which were further classified into 14 subfamilies. Gene structure analysis revealed that genes within the same subgroup shared similar exon/intron structures. A signature small peptide recognition motif (RxR) was found to be largely conserved within members of subfamily IX, suggesting that these members may recognize peptide signals as ligands. 26 of the 246 StLRR-RLK genes were found to have arisen from tandem or segmental duplication events. Expression profiling revealed that StLRR-RLK genes were differentially expressed in various organs/tissues, and several genes were found to be responsive to different stress treatments. Furthermore, StLRR-RLK117 was found to be able to form homodimers and heterodimers with StLRR-RLK042 and StLRR-RLK052. Notably, the overlapping expression region of StLRR-RLK117 with Solanum tuberosum WUSCHEL (StWUS) suggested that the CLV3–CLV1/BAM–WUS feedback loop may be conserved in potato to maintain stem cell homeostasis within the shoot apical meristem.

scholarly journals Investigation and Computational Analysis of the Sulfotransferase (SOT) Gene Family in Potato (Solanum tuberosum): Insights into Sulfur Adjustment for Proper Development and Stimuli Responses

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2597
Author(s):  
Sahar Faraji ◽  
Parviz Heidari ◽  
Hoorieh Amouei ◽  
Ertugrul Filiz ◽  
Abdullah ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Gene Family ◽  
Stress Responses ◽  
Active Sites ◽  
Sulfur Metabolism ◽  
Functional Characterization ◽  
Phosphate Binding ◽  
Protein Motifs ◽  
A Genome

Various kinds of primary metabolisms in plants are modulated through sulfate metabolism, and sulfotransferases (SOTs), which are engaged in sulfur metabolism, catalyze sulfonation reactions. In this study, a genome-wide approach was utilized for the recognition and characterization of SOT family genes in the significant nutritional crop potato (Solanum tuberosum L.). Twenty-nine putative StSOT genes were identified in the potato genome and were mapped onto the nine S. tuberosum chromosomes. The protein motifs structure revealed two highly conserved 5′-phosphosulfate-binding (5′ PSB) regions and a 3′-phosphate-binding (3′ PB) motif that are essential for sulfotransferase activities. The protein–protein interaction networks also revealed an interesting interaction between SOTs and other proteins, such as PRTase, APS-kinase, protein phosphatase, and APRs, involved in sulfur compound biosynthesis and the regulation of flavonoid and brassinosteroid metabolic processes. This suggests the importance of sulfotransferases for proper potato growth and development and stress responses. Notably, homology modeling of StSOT proteins and docking analysis of their ligand-binding sites revealed the presence of proline, glycine, serine, and lysine in their active sites. An expression essay of StSOT genes via potato RNA-Seq data suggested engagement of these gene family members in plants’ growth and extension and responses to various hormones and biotic or abiotic stimuli. Our predictions may be informative for the functional characterization of the SOT genes in potato and other nutritional crops.

scholarly journals Mitogen-Activated Protein Kinase Expression Profiling Revealed Its Role in Regulating Stress Responses in Potato (Solanum tuberosum)

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1371
Author(s):  
Madiha Zaynab ◽  
Athar Hussain ◽  
Yasir Sharif ◽  
Mahpara Fatima ◽  
Mateen Sajid ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Protein Kinase ◽  
Stress Responses ◽  
Expression Profiling ◽  
Regulatory Elements ◽  
Mitogen Activated Protein Kinase ◽  
High Expression ◽  
Primary Data ◽  
A Genome ◽  
Mitogen Activated Protein

Mitogen-activated protein kinase (MAPK) cascades are the universal signal transduction networks that regulate cell growth and development, hormone signaling, and other environmental stresses. However, their essential contribution to plant tolerance is very little known in the potato (Solanum tuberosum) plant. The current study carried out a genome-wide study of StMAPK and provided a deep insight using bioinformatics tools. In addition, the relative expression of StMAPKs was also assessed in different plant tissues. The similarity search results identified a total of 22 StMAPK genes in the potato genome. The sequence alignment also showed conserved motif TEY/TDY in most StMAPKs with conserved docking LHDXXEP sites. The phylogenetic analysis divided all 22 StMAPK genes into five groups, i.e., A, B, C, D, and E, showing some common structural motifs. In addition, most of the StMAPKs were found in a cluster form at the terminal of chromosomes. The promoter analysis predicted several stress-responsive Cis-acting regulatory elements in StMAPK genes. Gene duplication under selection pressure also indicated several purifying and positive selections in StMAPK genes. In potato, StMAPK2, StMAPK6, and StMAPK19 showed a high expression in response to heat stress. Under ABA and IAA treatment, the expression of the total 20 StMAPK genes revealed that ABA and IAA played an essential role in this defense process. The expression profiling and real-time qPCR (RT-qPCR) exhibited their high expression in roots and stems compared to leaves. These results deliver primary data for functional analysis and provide reference data for other important crops.

scholarly journals The biosynthetic pathway of potato solanidanes diverged from that of spirosolanes due to evolution of a dioxygenase

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ryota Akiyama ◽  
Bunta Watanabe ◽  
Masaru Nakayasu ◽  
Hyoung Jae Lee ◽  
Junpei Kato ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Lycopersicum ◽  
Biosynthetic Pathway ◽  
Potato Breeding ◽  
Chemical Diversity ◽  
Evolutionary Divergence ◽  
Food Crop ◽  
Common Pathway ◽  
Structural Differences ◽  
Key Enzyme

AbstractPotato (Solanum tuberosum), a worldwide major food crop, produces the toxic, bitter tasting solanidane glycoalkaloids α-solanine and α-chaconine. Controlling levels of glycoalkaloids is an important focus on potato breeding. Tomato (Solanum lycopersicum) contains a bitter spirosolane glycoalkaloid, α-tomatine. These glycoalkaloids are biosynthesized from cholesterol via a partly common pathway, although the mechanisms giving rise to the structural differences between solanidane and spirosolane remained elusive. Here we identify a 2-oxoglutarate dependent dioxygenase, designated as DPS (Dioxygenase for Potato Solanidane synthesis), that is a key enzyme for solanidane glycoalkaloid biosynthesis in potato. DPS catalyzes the ring-rearrangement from spirosolane to solanidane via C-16 hydroxylation. Evolutionary divergence of spirosolane-metabolizing dioxygenases contributes to the emergence of toxic solanidane glycoalkaloids in potato and the chemical diversity in Solanaceae.

Proteome characterization of copper stress responses in the roots of sorghum

BioMetals ◽  
2017 ◽  
Vol 30 (5) ◽  
pp. 765-785 ◽  
Author(s):  
Swapan Kumar Roy ◽  
Seong-Woo Cho ◽  
Soo Jeong Kwon ◽  
Abu Hena Mostafa Kamal ◽  
Dong-Gi Lee ◽  
...  
Keyword(s):  
Stress Responses ◽  
Copper Stress

Expression and Characterization of Rat Protein Phosphatases-1α, -1γ1, -1γ2, and -1δ

1993 ◽  
Vol 303 (2) ◽  
pp. 402-406 ◽  
Author(s):  
Z.J. Zhang ◽  
G. Bai ◽  
M. Shima ◽  
S.M. Zhao ◽  
M. Nagao ◽  
...  
Keyword(s):  
Protein Phosphatases

Distribution and molecular characterization of potato virus Y (PVY) strains infecting potato (Solanum tuberosum) crop in Kashmir (India)

VirusDisease ◽  
2021 ◽  
Author(s):  
Sumiah Wani ◽  
Sahar Saleem ◽  
Sajad U. Nabi ◽  
Gowhar Ali ◽  
Bilal A. Paddar ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y
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