Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum

Planta ◽  
2018 ◽  
Vol 249 (1) ◽  
pp. 235-249 ◽  
Author(s):  
Ashita Hamachi ◽  
Masahiro Nisihara ◽  
Shiori Saito ◽  
Hojun Rim ◽  
Hideyuki Takahashi ◽  
...  
Author(s):  
Lily N Edwards-Callaway ◽  
M Caitlin Cramer ◽  
Caitlin N Cadaret ◽  
Elizabeth J Bigler ◽  
Terry E Engle ◽  
...  

ABSTRACT Shade is a mechanism to reduce heat load providing cattle with an environment supportive of their welfare needs. Although heat stress has been extensively reviewed, researched, and addressed in dairy production systems, it has not been investigated in the same manner in the beef cattle supply chain. Like all animals, beef cattle are susceptible to heat stress if they are unable to dissipate heat during times of elevated ambient temperatures. There are many factors that impact heat stress susceptibility in beef cattle throughout the different supply chain sectors, many of which relate to the production system, i.e. availability of shade, microclimate of environment, and nutrition management. The results from studies evaluating the effects of shade on production and welfare are difficult to compare due to variation in structural design, construction materials used, height, shape, and area of shade provided. Additionally, depending on operation location, shade may or may not be beneficial during all times of the year, which can influence the decision to make shade a permanent part of management systems. Shade has been shown to lessen the physiologic response of cattle to heat stress. Shaded cattle exhibit lower respiration rates, body temperatures, and panting scores compared to un-shaded cattle in weather that increases the risk of heat stress. Results from studies investigating the provision of shade indicate that cattle seek shade in hot weather. The impact of shade on behavioral patterns is inconsistent in the current body of research, some studies indicating shade provision impacts behavior and other studies reporting no difference between shaded and un-shaded groups. Analysis of performance and carcass characteristics across feedlot studies demonstrated that shaded cattle had increased ADG, improved feed efficiency, HCW, and dressing percentage when compared to cattle without shade. Despite the documented benefits of shade, current industry statistics, although severely limited in scope, indicate low shade implementation rates in feedlots and data in other supply chain sectors do not exist. Industry guidelines and third party on-farm certification programs articulate the critical need for protection from extreme weather but are not consistent in providing specific recommendations and requirements. Future efforts should include: updated economic analyses of cost versus benefit of shade implementation, exploration of producer perspectives and needs relative to shade, consideration of shade impacts in the cow-calf and slaughter plant segments of the supply chain, and integration of indicators of affective (mental) state and preference in research studies to enhance the holistic assessment of cattle welfare.


2016 ◽  
Vol 67 (4) ◽  
pp. 317 ◽  
Author(s):  
Malcolm J. Morrison ◽  
Allison Gutknecht ◽  
John Chan ◽  
S. Shea Miller

If predictions are correct, heat stress during reproduction will become a yield limiting factor in many world crops and breeding heat stress tolerance a major goal. The objective of our paper was to highlight a novel system to investigate the influence of temperature (T) on pollen germination using a thermal gradient PCR programmed to establish differential Ts across 12 wells of a PCR plate. Seven cultivars of Brassica napus L. were grown through flowering in a cool growth cabinet (20/15°C day/night) or a heat stress cabinet (HST, 27/22°C day/night). Pollen from each cultivar × cabinet combination was aspirated from 6 opened flowers, and suspended in germination media. Drops of the pollen suspension were floated on media in each well, and the PCR T was set to 30°C with a gradient of ± 10°C, creating a range from ~20 to 40°C from left to right. After an 8 h treatment, the pollen germination (pg, %) and pollen tube growth score (ptg, 1–5) were evaluated using a microscope. There were significant differences among cultivars for pg and ptg score and significant differences among well T for pg and ptg score. Pollen tubes grew best at T from 20 to 23°C. Well T exceeding 33°C reduced pg and ptg score, although 3 of the 8 cultivars had good pg even at 36°C. HST >29°C, in a growth cabinet, generally resulted in B. napus raceme sterility, although our experiment showed that pollen was still capable of germinating up to 33°C, indicating that pollen germination may not be the only reason for heat stress susceptibility.


2012 ◽  
Vol 56 (3) ◽  
pp. 422-430 ◽  
Author(s):  
H. Gong ◽  
G. Chen ◽  
F. Li ◽  
X. Wang ◽  
Y. Hu ◽  
...  

Author(s):  
R. Sunil ◽  
A.K. Chhabra ◽  
Rajesh Kumar Yadav ◽  
Sunil Kumar

Background: Heat stress is a major restrain in chickpea (Cicer arietinum L.) productivity. Developing tolerant chickpea genotypes contributes breeding materials for hybridization programme. Stress indices related to abiotic stresses are found effective in screening of genotypes for high temperature tolerance in chickpea. Methods: An experiment with 24 genotypes under two different environments i.e., timely and late sown conditions was planned to identify the chickpea genotypes tolerant to heat stress, using thirteen stress indices. Stress Susceptibility Index (SSI) is the cardinal index to group genotypes based on their tolerance level. Stress indices Mean Productivity (MP), Geometric Mean Productivity (GMP), Harmonic Mean (HM) Index, Heat Resistant Index (HRI) and Modified Heat Tolerance Index (MHTI) are very effective in identifying stress tolerant genotypes. Result: The result indicated that genotypes H 04-75, H 08-75, H 12-26, H 09-96, ICCV 92944, DCP 92-3 and GNG 2226 are tolerant to heat stress. The identified genotypes can be used as parents in hybridization programme for breeding chickpea cultivars tolerant to high temperature environments.


2021 ◽  
Author(s):  
Hannah E Krawczyk ◽  
Alexander Helmut Rotsch ◽  
Cornelia Herrfurth ◽  
Patricia Scholz ◽  
Orr Shomroni ◽  
...  

After reaching the stigma, pollen grains germinate and form a pollen tube that transports the sperm cells to the ovule. Due to selection pressure between pollen tubes, they likely evolved mechanisms to quickly adapt to temperature changes to sustain an elongation at the highest possible rate. We investigated these adaptions in Nicotiana tabacum pollen tubes grown in vitro under 22 °C and 37 °C by a multi-omic approach including lipidomic, metabolomic and transcriptomic analysis. Both glycerophospholipids and galactoglycerolipids increased in saturated acyl chains under heat stress while triacylglycerols changed less in respect to desaturation but showed higher levels. Free sterol composition was altered, and sterol ester levels decreased. The levels of sterylglycosides and several sphingolipid classes and species were augmented. Most amino acids increased during heat stress, including the non-codogenic amino acids γ-amino butyrate and pipecolate. Furthermore, the sugars sedoheptulose and sucrose showed higher levels. Also the transcriptome underwent pronounced changes with 1,570 of 24,013 genes being differentially up- and 813 being downregulated. Transcripts coding for heat shock proteins and many transcriptional regulators were most strongly upregulated, but also transcripts that have so far not been linked to heat stress. Transcripts involved in triacylglycerol synthesis were increased, while the modulation of acyl chain desaturation seemed not to be transcriptionally controlled indicating other means of regulation.


2013 ◽  
Vol 199 (5) ◽  
pp. 340-350 ◽  
Author(s):  
S. Shanmugam ◽  
K. H. Kjaer ◽  
C.-O. Ottosen ◽  
E. Rosenqvist ◽  
D. Kumari Sharma ◽  
...  

1997 ◽  
Vol 20 (12) ◽  
pp. 1534-1542 ◽  
Author(s):  
A. SMERTENKO ◽  
1,2 P. DRABER ◽  
V. VIKLICKY ◽  
Z. OPATRNY

2016 ◽  
Vol 141 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Sanalkumar Krishnan ◽  
Yingmei Ma ◽  
Emily Merewitz

Mowing frequencies are associated with differences in disease susceptibility of turfgrasses, but how hormones respond to mowing practices are not fully understood. Two independent growth chamber experiments were conducted to determine how leaf trimming and heat stress play a role in modulating endogenous hormones within creeping bentgrass (Agrostis stolonifera) leaf tissues. The study also aimed to evaluate whether there are hormone changes at 0, 15, and 30 minutes after leaf trimming (wounding). The effects of trimming and temperature on sod plugs of creeping bentgrass ‘Penncross’ and ‘Penn-G2’ were investigated under optimal conditions (23/20 °C day/night) and heat stress (30/25 °C day/night). Plants were 1) untrimmed and sampled by plucking at the leaf base, 2) untrimmed and sampled by cutting at 0, 15, and 30 minutes, or 3) trimmed once every 3 days. Salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), and indole-3-acetic acid (IAA) were generally greater in untrimmed plants compared with plants trimmed every 3 days under high temperature conditions. Zeatin riboside (ZR) was lower in untrimmed plants compared with plants trimmed every 3 days. JA and gibberellic acid (GA) accumulated to greater levels in the plants after 15 and 30 minutes of sampling. Polyamines (PAs) exhibited a transient increase in putrescine (Put) due to wounding. The results demonstrate the importance of research practices that consider the timing of sampling turfgrass plants for hormone analysis, help elucidate why mowing practices may play a role in stress susceptibility, and may be applicable to various studies related to leaf wounding.


Animals ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 173 ◽  
Author(s):  
Jill Nelson ◽  
Don McIntyre ◽  
Hilary Pavlidis ◽  
Gregory Archer

Reducing stress is an important goal in animal production. Previous research has demonstrated the ability of Original XPCTM to reduce the stress response of broilers during heat stress. Three trials were conducted to evaluate the effects of adding Original XPCTM to the feed or AviCareTM to the water on stress susceptibility of broiler chickens. Treatments included: control nonstressed (CNS), control stressed (CS), stressed with Original XPCTM (1.25 kg/metric ton feed, 0–42 days; XPC), and stressed with AviCareTM (160 mL/100 L drinking water, 0–42 days; AVI). All stressed treatments received the following stressors: live coccidiosis vaccination (day 1), reared on reused litter (days 0–42), and heat stress with feed/water withdrawal (12 h on day 18). Plasma corticosterone and heterophil/lymphocyte (H/L) ratio were determined from 60 birds/T on day 19, and 24 birds/T on day 41. Physical asymmetry was determined using bilateral bone measurements from 60 birds/T on day 41. Birds provided XPC or AVI had lower corticosterone and H/L ratios than CS (p < 0.05) on day 19 and lower corticosterone, H/L ratios, and asymmetry scores than both CNS and CS on day 41 (p < 0.05) in all three trials. Supplementing XPC or AVI improved broiler welfare measured by reduced stress indicators after acute heat stress or normal rearing stress in all trials.


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