Abstract
Background: Soil salinization and alkalinization are the main factors that affect the agricultural productivity in the world. Evaluating the persistence of the modifier applied in field soils is an important part of the regulation of saline stress and alkaline stress. Result: To determine the molecular mechanism of cotton’s responses to the regulation of saline stress and alkaline stress by the modifier, in this study, cotton was planted in the salinized soil (NaCl 8g kg-1) and alkalized soil (Na2CO3 8g kg-1) after application of the modifier, and ion content, physiological characteristics, and transcription and sequencing of new leaves during the flowering and boll-forming stage of cotton were analyzed. The results showed that compared with saline stress, alkaline stress increased the content of Na+, K+, SOD, and MDA in leaves, and the application of modifier reduced the content of Na+ but increased the K+/Na+ ratio, the activities of SOD, POD, CAT, and REC. Transcriptome analysis revealed that after the application of the modifier, the Na+/H+ exchanger gene in cotton leaves was down-regulated, the K+ transporter, K+ channel and POD genes were up-regulated. Besides, the down-regulation of genes related to lignin synthesis in phenylalanine biosynthesis pathway was consistent with the study results of ion content and physiological characteristics in leaves. The quantitative analysis with PCR proved the reliability of the results of RNA sequencing.Conclusion: These findings indicate that the modifier alleviated saline stress and alkaline stress on cotton by regulating candidate genes in key biological pathways, which improves our understanding of the molecular mechanism of the modifier regulating saline stress and alkaline stress.
Changes in the Leaf Physiological Characteristics and Tissue-Specific Distribution of Ginsenosides in Panax ginseng During Flowering Stage Under Cold Stress
