Secreted proteins produced by fungi associated with Botryosphaeria dieback trigger distinct defense responses in Vitis vinifera and Vitis rupestris cells

PROTOPLASMA ◽  
2017 ◽  
Vol 255 (2) ◽  
pp. 613-628 ◽  
Author(s):  
E. Stempien ◽  
M.-L. Goddard ◽  
Y. Leva ◽  
M. Bénard-Gellon ◽  
H. Laloue ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Defense Responses ◽  
Secreted Proteins ◽  
Vitis Rupestris ◽  
Botryosphaeria Dieback

scholarly journals Transcriptome Analysis and Cell Morphology of Vitis rupestris Cells to Botryosphaeria Dieback Pathogen Diplodia seriata

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 179
Author(s):  
Liang Zhao ◽  
Shuangmei You ◽  
Hui Zou ◽  
Xin Guan
Keyword(s):  
Molecular Mechanisms ◽  
Defense Responses ◽  
Physiological Status ◽  
Suspension Cells ◽  
Diplodia Seriata ◽  
Vitis Rupestris ◽  
Leaf Spots ◽  
Wild Grapevine ◽  
Botryosphaeria Dieback ◽  
Plant Pathogen Interaction

Diplodia seriata, one of the major causal agents of Botryosphaeria dieback, spreads worldwide, causing cankers, leaf spots and fruit black rot in grapevine. Vitis rupestris is an American wild grapevine widely used for resistance and rootstock breeding and was found to be highly resistant to Botryosphaeria dieback. The defense responses of V. rupestris to D. seriata 98.1 were analyzed by RNA-seq in this study. There were 1365 differentially expressed genes (DEGs) annotated with Gene Ontology (GO) and enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The DEGs could be allocated to the flavonoid biosynthesis pathway and the plant–pathogen interaction pathway. Among them, 53 DEGs were transcription factors (TFs). The expression levels of 12 genes were further verified by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The aggregation of proteins on the plasma membrane, formation variations in the cytoskeleton and plasmodesmata and hormone regulations revealed a declined physiological status in V. rupestris suspension cells after incubation with the culture filtrates of D. seriata 98.1. This study provides insights into the molecular mechanisms in grapevine cells’ response to D. seriata 98.1, which will be valuable for the control of Botryosphaeria dieback.

scholarly journals Colonization of Solanum melongena and Vitis vinifera Plants by Botrytis cinerea Is Strongly Reduced by the Exogenous Application of Tomato Systemin

2020 ◽  
Vol 7 (1) ◽  
pp. 15
Author(s):  
Donata Molisso ◽  
Mariangela Coppola ◽  
Anna Maria Aprile ◽  
Concetta Avitabile ◽  
Roberto Natale ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Botrytis Cinerea ◽  
Signaling Pathway ◽  
Intracellular Signaling ◽  
Crop Protection ◽  
Solanum Melongena ◽  
Grey Mould ◽  
Defense Responses ◽  
Wound Response ◽  
Intracellular Signaling Pathway

Plant defense peptides are able to control immune barriers and represent a potential novel resource for crop protection. One of the best-characterized plant peptides is tomato Systemin (Sys) an octadecapeptide synthesized as part of a larger precursor protein. Upon pest attack, Sys interacts with a leucine-rich repeat receptor kinase, systemin receptor SYR, activating a complex intracellular signaling pathway that leads to the wound response. Here, we demonstrated, for the first time, that the direct delivery of the peptide to Solanum melongena and Vitis vinifera plants protects from the agent of Grey mould (Botrytis cinerea). The observed disease tolerance is associated with the increase of total soluble phenolic content, the activation of antioxidant enzymes, and the up-regulation of defense-related genes in plants treated with the peptide. Our results suggest that in treated plants, the biotic defense system is triggered by the Sys signaling pathway as a consequence of Sys interaction with a SYR-like receptor recently found in several plant species, including those under investigation. We propose that this biotechnological use of Sys, promoting defense responses against invaders, represents a useful tool to integrate into pest management programs for the development of novel strategies of crop protection.

scholarly journals Rhizogénèse de bourgeons apicaux de boutures de vigne cultivées in vitro

OENO One ◽  
1979 ◽  
Vol 13 (2) ◽  
pp. 125
Author(s):  
Serge Grenan
Keyword(s):  
Heat Treatment ◽  
Vitis Vinifera ◽  
Technical Difficulty ◽  
The Other ◽  
Standard Medium ◽  
Rooted Cuttings ◽  
Vitis Rupestris ◽  
Petite Taille ◽  
Vegetative Multiplication

<p style="text-align: justify;">La multiplication végétative de la vigne cultivée <em>in vitro</em> à partir de boutures à un œil ayant 1 cm de longueur ne présente aucune difficulté technique. Par contre la propagation d'implants de plus petite taille s'avère délicate et aléatoire. Or l'élimination durable de virus thermorésistants nécessite le prélèvement (à la fin du thermotraitement) de très petites parties terminales de tiges.</p><p style="text-align: justify;">Des « boutures apicales » de 2 à 5 mm de longueur de <em>Vitis Vinifera</em> variété Grenache N, et de <em>Vitis Rupestris</em>, variété Lot ont été mises en culture sur un milieu de base additionné de diverses substances de croissance. L'AIA à la concentration 10-<sup>7</sup>M s'est révélé le régulateur favorisant le mieux la rhizogénèse des « boutures apicales ». Le transfert des « boutures apicales » enracinées sur le milieu de référence facilite leur croissance.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Vegetative multiplication of vine grown <em>in vitro</em> from one-eyed-cuttings one centimeter long, does not present any technical difficulty. On the other hand, propagation of smaller implants is difficult and (random). The trouble is durable elimination of thermoresistant virus needs picking up (at the end of heat treatment) very small shoot tips.</p><p style="text-align: justify;">Apical cuttings of <em>Vitis Vinifera</em>, var. Grenache N. and of <em>Vitis Rupestris</em>, var. Lot two or five millimeters long are grown on a standard medium in which different growing substances are added. AIA at the concentration of 10-<sup>7</sup>M increases the best rhizogenesis of apical cuttings. The transfer of rooted cuttings upon the standard medium makes their growth easier.</p>

scholarly journals Microfluidic synthesis of methyl jasmonate-loaded PLGA nanocarriers as a new strategy to improve natural defenses in Vitis vinifera

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Laura Chronopoulou ◽  
Livia Donati ◽  
Marco Bramosanti ◽  
Roberta Rosciani ◽  
Cleofe Palocci ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Methyl Jasmonate ◽  
Fluid Dynamic ◽  
Biological Response ◽  
Defense Responses ◽  
Cell Uptake ◽  
Biological Applications ◽  
New Strategy ◽  
Endogenous Elicitor ◽  
Stilbene Biosynthesis

AbstractThe objective of the present work was to synthesize biopolymeric nanoparticles (NPs) entrapping the resistance-inductor methyl jasmonate (MeJA) to be employed as a novel and alternative strategy in integrated pest management. NPs were prepared by using a continuous flow microfluidic reactor that allows to precisely control some features that are crucial for applications such as size, polydispersion, morphology and reproducibility. Poly(lactic-co-glycolic acid) (PLGA), a biopolymer largely studied for its use in biological applications, was chosen for the production of NPs entrapping MeJA, a biotic endogenous elicitor able to trigger plant’s defense responses. The effect of different fluid-dynamic conditions, PLGA molecular weight and concentration on NP properties (dimensions, polydispersion, morphology, stability) was evaluated. DLS and SEM were employed to characterize the obtained NPs. MeJA-loaded PLGA NPs ranging from 40 to 70 nm were administered to Vitis vinifera cell cultures, in order to evaluate the biological response in terms of stilbene biosynthesis. HPLC investigations showed a faster response when the elicitor was administered by PLGA NPs in comparison with free MeJA. This result demonstrates that the encapsulation in PLGA NPs significantly promotes MeJA cell uptake and the activation of MeJA-induced responses.

scholarly journals Wood Metabolomic Responses of Wild and Cultivated Grapevine to Infection with Neofusicoccum parvum, a Trunk Disease Pathogen

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 232 ◽  
Author(s):  
Clément Labois ◽  
Kim Wilhelm ◽  
Hélène Laloue ◽  
Céline Tarnus ◽  
Christophe Bertsch ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Strong Increase ◽  
Primary Metabolism ◽  
Alcohol Content ◽  
Neofusicoccum Parvum ◽  
Specialized Metabolites ◽  
Grapevine Trunk Diseases ◽  
Botryosphaeria Dieback ◽  
Trunk Diseases ◽  
Resveratrol Oligomer

Grapevine trunk diseases (GTDs), which are associated with complex of xylem-inhabiting fungi, represent one of the major threats to vineyard sustainability currently. Botryosphaeria dieback, one of the major GTDs, is associated with wood colonization by Botryosphaeriaceae fungi, especially Neofusicoccum parvum. We used GC-MS and HPLC-MS to compare the wood metabolomic responses of the susceptible Vitis vinifera subsp. vinifera (V. v. subsp. vinifera) and the tolerant Vitis vinifera subsp. sylvestris (V. v. subsp. sylvestris) after artificial inoculation with Neofusicoccum parvum (N. parvum). N. parvum inoculation triggered major changes in both primary and specialized metabolites in the wood. In both subspecies, infection resulted in a strong decrease in sugars (fructose, glucose, sucrose), whereas sugar alcohol content (mannitol and arabitol) was enhanced. Concerning amino acids, N. parvum early infection triggered a decrease in aspartic acid, serine, and asparagine, and a strong increase in alanine and β-alanine. A trend for more intense primary metabolism alteration was observed in V. v. subsp. sylvestris compared to V. v. subsp. vinifera. N. parvum infection also triggered major changes in stilbene and flavonoid compounds. The content in resveratrol and several resveratrol oligomers increased in the wood of both subspecies after infection. Interestingly, we found a higher induction of resveratrol oligomer (putative E-miyabenol C, vitisin C, hopeaphenol, ampelopsin C) contents after wood inoculation in V. v. subsp. sylvestris.

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