scholarly journals Effects of different types of salts on the growth of lactic acid bacteria and yeasts during kimchi fermentation

2017 ◽  
Author(s):  
Kang Wook Lee ◽  
Jae Min Shim ◽  
Dong Wook Kim ◽  
Zhuang Yao ◽  
Jeong A. Kim ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Different Types

scholarly journals Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources

2013 ◽  
Vol 31 (No. 4) ◽  
pp. 323-331 ◽  
Author(s):  
P. Khemariya ◽  
S. Singh ◽  
G. Nath ◽  
A.K. Gulati
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antimicrobial Agent ◽  
Lactococcus Lactis ◽  
Antibiotic Susceptibility ◽  
Pcr Amplification ◽  
Food Borne ◽  
Antibiotic Susceptibility Test ◽  
Different Types ◽  
Zones Of Inhibition

Eight isolates of Lactococcus lactis subsp. lactis were isolated and identified by phenotypic and molecular characterisation out of 23 isolates of lactic acid bacteria (LAB) from different dairy and non-dairy sources. Out of eight strains, four were obtained from dairy and four from non-dairy sources. All eight strains of L. lactis subsp. lactis were able to produce zones of inhibition against the Lactobacillus acidophilus NCDC 015. The antimicrobial agent produced by the isolates inhibited the growth of a range of related lactic acid bacteria and certain Gram positive food-borne microorganisms. The antimicrobial agent, i.e. nisin, produced by the strains was confirmed by PCR amplification of nisin gene sequences of 174 bp size. Antibiotic susceptibility test to 21 different types of antibiotics was evaluated. All the isolates were resistant to fosfomycin, cefepime, amikacin, kanamycin, neomycin, nalidixic acid, pipemidic acid, norfloxacin, sulphadiazine, colistin, polymixin, teicoplanin, nystatin, and amphotericin B but susceptible to ampicillin, erythromycin, spiramycin, spectinomycin, ciprofloxacin, rifampicin, and trimethoprim.  

Plasmid contents of lactic acid bacteria isolated from different types of sour doughs

1990 ◽  
Vol 20 (4) ◽  
pp. 201-207 ◽  
Author(s):  
Clas Lönner ◽  
Katarina Preve-Åkesson ◽  
Siv Ahrné
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Different Types

scholarly journals Optimization of Bacterial Concentrate Media for Lactobacillus Sakei

2022 ◽  
Author(s):  
Nikiforova AP ◽  
Khazagaeva SN ◽  
Khamagaeva IS
Keyword(s):  
Lactic Acid ◽  
Growth Factors ◽  
Lactic Acid Bacteria ◽  
Rice Flour ◽  
Good Growth ◽  
Lactobacillus Sakei ◽  
Factorial Experiments ◽  
Bacterial Strains ◽  
Fish Products ◽  
Different Types

Two strains of lactic acid bacteria were selected for the study (Lactobacillus sakei Lsk-45andLactobacillus sakeiDSM 20017). Bacterial media, based on rice and rice flour, were tested as an alternative to media based on whey. A comparison of the different types of media showed that there was better growth of the selected strains on themedium based on rice flour.Statistical analyses, including factorial experiments and response surface analyses, were used to optimizethe composition of the bacterial medium for Lactobacillus sakei propagation. Bacteriological peptone and rice flour were found to be good growth factors for Lactobacillus sakei.For Lactobacillus sakei Lsk-45,better growth was obtained with the use of 7.75-10 g/L of peptone and 57.5-75 g/L of rice flour. For Lactobacillus sakei DSM 20017, better growth was obtained with the use of 7-10 g/L of peptone and 40-75 g/L of rice flour. Keywords: starters, media, Lactobacillus sakei, fermentation,fish products, bacterial strains, lactic acid bacteria

scholarly journals Stability of Lactic Acid Bacteria in Pasteurized Cow’s and Goat’s Milk

2019 ◽  
Vol 1 (4) ◽  
pp. 77-82
Author(s):  
Nurfarhana Syed Malik ◽  
Mohd. Nizam Lani ◽  
Fauziah Tufail Ahmad
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Viable Count ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Goat’S Milk ◽  
Different Types ◽  
Goat's Milk ◽  
The Stability

This study was done to determine the effect of pasteurization on the stability of lactic acid bacteria (LAB) and its enzyme in raw and pasteurized cow’s and goat’s milk. The total viable count for plate count of the bacterial growth concentration was higher in both pasteurized cow’s and goat’s milk at 2.48 log CFU/ml. This is followed by raw cow’s milk (1.59 log CFU/ml) and raw goat’s milk (0.65 log CFU/ml). Lactic acid bacteria (LAB) was found to be similar in both raw cow’s and goat’s milk (p>0.05), and pasteurized milk of both animals also contained the same amount of LAB (p>0.05). LAB was still detected in pasteurized milk (p<0.05), indicating the stability of LAB against the pasteurization temperature. Interestingly, based on API ZYM assay kit results, there were nine different enzymes detected in all samples, which were leucinearylamidase, valinearylamidase,cystinearylamidase, trypsin, α-chymotrypsin, naphthol-AS-BI-phosphohydrolase, α-glucosidase, β-glucosidaseand acid phosphatise. The results revealed that different types of lactic acid bacteria were detected in treated and non-treated milk samples produced by different animals, indicating the different stability levels of LAB against pasteurization.

scholarly journals Characterization of Dadih: Traditional Fermented Buffalo Milk of Minangkabau

Beverages ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 60
Author(s):  
Marcellus Arnold ◽  
Yolanda Victoria Rajagukguk ◽  
Anna Gramza-Michałowska
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Buffalo Milk ◽  
Aminobutyric Acid ◽  
Spontaneous Fermentation ◽  
Functional Value ◽  
Different Types ◽  
Stress Agent ◽  
Fermentation Period

Dadih or dadiah is traditional fermented buffalo milk of Minangkabau, which occurs spontaneously. Dadih is commonly served as ampiang dadih, or other dishes. The microbiota found in dadih are dominated by lactic acid bacteria, and yeasts are also found. The lactic acid bacteria provide functional value, such as antimicrobial, hypocholesterolemic, antimutagenic, antioxidant, and immunomodulatory properties, as well as being the source of γ-aminobutyric acid (GABA) as an anti-stress agent and folate. Nevertheless, many challenges were observed in dadih production, including the limitation of buffalo milk production due to decreasing populations of buffalo in the last two decades, unstandardized dadih production due to the spontaneous fermentation in natural bamboo tubes, and safety problems as no heat treatment is applied in the production of dadih. These problems impede the development of dadih production, thus is it important to improve buffalo cultivation through artificial insemination programs, using different types of milk and pasteurization processes in dadih production, and incubator development to accelerate the fermentation period.

Population Dynamics of the Constitutive Biota of French Dry Sausages in a Pilot-Scale Ripening Chamber†

2004 ◽  
Vol 67 (10) ◽  
pp. 2306-2309 ◽  
Author(s):  
RAQUEL HUERTA ◽  
R. JORDANO ◽  
L. M. MEDINA ◽  
CARMEN LÓPEZ
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Dominant Species ◽  
Pilot Scale ◽  
Lactobacillus Sakei ◽  
Pediococcus Acidilactici ◽  
Lactobacillus Curvatus ◽  
Ripening Process ◽  
Different Types ◽  
Dry Sausages

The population dynamic of constitutive biota on 84 samples belonging to two different types of French fermented dry sausages during the ripening process in a pilot-scale ripening chamber was investigated. Samples were analyzed in three steps of their production: fresh product, first drying stage, and finished product. In addition, 180 strains of lactic acid bacteria were identified using a miniaturized biochemical procedure of characterization. In general, the number of lactic acid bacteria that evolved during the ripening process of French dry sausages increased during the first days of the process after which the number of these organisms remained constant at approximately 8 log CFU/g. Lactobacillus sakei and Pediococcus pentosaceus, bacteria added as starter, were the dominant species. Pediococcus urinaeequi, Pediococcus acidilactici, and particularly Lactobacillus curvatus were also present. Finally, we have to take into account that the controlled conditions of the pilot plant generally contribute to the homogenization of the behavior of the starter biota.

scholarly journals Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production

2021 ◽  
Vol 8 (6) ◽  
pp. 100
Author(s):  
Ehsan Oskoueian ◽  
Mohammad Faseleh Jahromi ◽  
Saeid Jafari ◽  
Majid Shakeri ◽  
Hieu Huu Le ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rice Straw ◽  
Methane Production ◽  
Dry Matter ◽  
In Vitro Study ◽  
Microbial Populations ◽  
Different Types ◽  
Silage Quality

Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; L. plantarum, L. salivarius, L. reuteri, L. brevi, and S. bovis) inoculation (106 cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved (p < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (p < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for L. plantarum and S. bovis) improved silage quality, rumen fermentation parameters and microbial populations in vitro.

scholarly journals PENGARUH PERLAKUAN 3 JENIS BAKTERI ASAM LAKTAT DAN KOMBINASINYA TERHADAP KARAKTERISTIK KEJU KEDELAI

2020 ◽  
Vol 9 (4) ◽  
pp. 412
Author(s):  
Nurgrahadi . ◽  
Ni Nyoman Puspawati ◽  
I Made Sugitha
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Moisture Content ◽  
Protein Content ◽  
Lactococcus Lactis ◽  
Combination Treatment ◽  
Lactobacillus Bulgaricus ◽  
Randomized Design ◽  
Different Types ◽  
Completely Randomized Design

The aims of this research was to determine the effect of using 3 different types of lactic acid bacteria and their combinations for the characteristics of soy cheese. This research used a Completely Randomized Design (CRD) with 7 treatments, namely Lactobacillus bulgaricus, Lactococcus lactis and Lactobacillus paracasei spp. paracasei 1 SKG44, which is a single starter, and several combinations consisting of L. bulgaricus with L. lactis; L. bulgaricus with L. paracasei spp. paracasei 1 SKG44; L. lactis with L. paracasei spp. paracasei 1 SKG44; and L. bulgaricus, L. lactis, and L. paracasei spp. paracasei 1 SKG44. The concentration of each treatment was 6%. The treatment was repeated 3 times to make 21 experimental units. The data was obtained by variance analysis and if there was an influence between treatments continued with the Duncan test. The results showed that the addition of different starter combinations would affected the characteristic of soy cheese. The combination treatment of L. bulgaricus, L. lactis, and L. paracasei spp. paracasei 1 SKG44 was produced the best soy cheese with a yield of 27,47%; 17, 02% protein content; 4,1 x 107 CFU/g total LAB; 0,13% total lactic acid; 79,10% moisture content; color rather white; texture soft; aroma rather typical of soybeans, flavor rather like and overall acceptance like.

scholarly journals BIOSAFETY ASSESSMENT OF FLOUR AND YEASTS FOR BAKERY NEEDS ACCORDING TO MICROBIOLOGICAL INDICATORS

2021 ◽  
Vol 2 (37) ◽  
pp. 128-131
Author(s):  
I. Коrnienko
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nutritional Value ◽  
Poor Quality ◽  
Bakery Products ◽  
Gluten Free ◽  
Different Types ◽  
Unleavened Bread ◽  
Microbiological Parameters ◽  
Pure Cultures

The problem of non-compliance with microbiological safety indicators of finished bakery products and flour of different types is formulated. It is noted that the reason for the development of bread disease, which is caused by pathogenic and opportunistic microorganisms, is poor quality flour. The solution of the problem is offered by use of symbiosis of lactic acid bacteria as a part of leaven which possess antagonistic properties in relation to many activators.                According to the results of microbiological research, pathogens of bread disease in wheat, spelled, oat and rye types of flour have been identified.The pathogenic micro­organisms present in the flour lead to accelerated spoilage of bread, reducing its nutritional value and safety in terms of microbiological parameters.                It is proposed to test the effectiveness of the use of biotechnological principles, which are based on the antagonistic properties of lactic acid bacteria. To determine the biosafety of the obtained yeast, microbiological studies were performed by seeding yeast samples on elective nutrient media Saburo, Blikfeldt, GMF agar, bifido- and lactoagar.                An improved leaven formulation for baking needs with an increased titer of lactic acid bacteria, which have powerful antagonistic powers, has been developed. Experiments have shown the effectiveness of the use of symbiosis of pure cultures of lactic acid bacteria in the composition of leavens to suppress diseases of bread, namely pathogens  – Aspergillus niger, Mycormucedoand Bacillus subtilis.             The use of unleavened leavens with a high content of pure cultures of lactic acid bacteria in the practice of baking unleavened bread based on gluten-free types of flour contributes to the expansion of the range of functional bakery products.

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