High prevalence of the PER-1 gene among carbapenem-resistant Acinetobacter baumannii in Riyadh, Saudi Arabia

2016 ◽  
Vol 35 (11) ◽  
pp. 1759-1766 ◽  
Author(s):  
M. M. Aly ◽  
N. M. Abu Alsoud ◽  
M. S. Elrobh ◽  
S. M. Al Johani ◽  
H. H. Balkhy
Keyword(s):  
Saudi Arabia ◽  
Acinetobacter Baumannii ◽  
Carbapenem Resistant ◽  
High Prevalence

scholarly journals Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals

2014 ◽  
Vol 63 (11) ◽  
pp. 1460-1466 ◽  
Author(s):  
Abdulrahman A. Alsultan ◽  
Elsayed Aboulmagd ◽  
Benjamin A. Evans ◽  
Sebastian G. B. Amyes
Keyword(s):  
Health Care ◽  
Saudi Arabia ◽  
Acinetobacter Baumannii ◽  
Insertion Sequence ◽  
Clonal Diversity ◽  
Diabetic Patients ◽  
Major Health ◽  
Intensive Care Patients ◽  
Carbapenem Resistant ◽  
Vitek 2

Carbapenem-resistant Acinetobacter baumannii (CR-AB) represents a major health-care problem, causing high rates of morbidity and mortality. This study investigated the clonality of CR-AB isolated from diabetic patients from different regions in Saudi Arabia, as well as the relatedness of the β-lactamase genes. A total of 64 non-repetitive CR-AB clinical isolates were collected from 16 different regions in Saudi Arabia from intensive care patients. Isolates were identified phenotypically by the Vitek 2 compact system and genotypically by amplification of the bla OXA-51-like gene. The target sequences were amplified by PCR and the clonal diversity of the isolates was explored by PFGE. Resistance studies revealed that the prevalence of imipenem and meropenem resistance was 92 % and 96 %, respectively, while the vast majority of the isolates were susceptible to tigecycline and colistin. In addition, bla VIM and bla OXA-23 were the most prevalent genes in the isolates under investigation, while ISAba1 was the most dominant insertion sequence. PFGE results showed 13 clusters; clone H was dominant, comprising 20 isolates from four hospitals, followed by clones C and F, comprising 11 isolates each from three and six hospitals, respectively. Moreover, the current study signified the clonal diversity of CR-AB in Saudi Arabia and showed the ability of some clones to infect patients in many different cities.

Carbapenem-susceptible Acinetobacter baumannii carrying the ISAba1 upstream blaOXA-51-like gene in Porto Alegre, southern Brazil

2012 ◽  
Vol 141 (2) ◽  
pp. 330-333 ◽  
Author(s):  
M. PAGANO ◽  
A. F. MARTINS ◽  
A. B. M. P. MACHADO ◽  
J. BARIN ◽  
A. L. BARTH
Keyword(s):  
Antimicrobial Resistance ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
High Morbidity ◽  
Southern Brazil ◽  
Porto Alegre ◽  
Integrase Gene ◽  
Carbapenem Resistant ◽  
High Prevalence ◽  
Class 1

SUMMARYOver the last decade, Acinetobacter baumannii resistant to carbapenems has emerged in many medical centres and is commonly associated with high morbidity and mortality. We investigated potential mechanisms contributing to antimicrobial resistance of 58 clinical isolates of A. baumannii collected during a prolonged city-wide outbreak in five different hospitals in southern Brazil. The integrase gene was detected in 51 (87·9%) isolates of which 36 harboured class 2 integrons alone and 14 had both class 1 and 2 integrons; all carbapenem-resistant isolates displayed class 2 integrons. ISAba1 was found upstream of blaOXA-23-like only in isolates resistant to carbapenems; however, ISAba1 upstream of blaOXA-51-like was present in both susceptible and resistant isolates. This is the first report of a high prevalence of class 2 integrons in A. baumannii in southern Brazil. Moreover, our study suggests that ISAba1/blaOXA-51-like alone is insufficient to confer resistance to carbapenems.

scholarly journals High frequency of carbapenem-resistant Acinetobacter baumannii in patients with diabetes mellitus in Saudi Arabia

2013 ◽  
Vol 62 (6) ◽  
pp. 885-888 ◽  
Author(s):  
A. A. Alsultan ◽  
B. A. Evans ◽  
E. A. Elsayed ◽  
S. I. Al-Thawadi ◽  
A. Y. Al-Taher ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Saudi Arabia ◽  
Acinetobacter Baumannii ◽  
Disease Status ◽  
Resistance Mechanisms ◽  
Diabetic Patients ◽  
Carbapenem Resistant ◽  
Patients With Diabetes ◽  
Relationship Of ◽  
The Relationship

Carbapenem-resistant Acinetobacter baumannii is becoming increasingly prevalent in patients with diabetes mellitus in the Middle East. We examined the relationship of these bacteria and their resistance mechanisms to the diabetic disease status of patients in Saudi Arabia. Susceptibilities of 271 isolates to carbapenems, tigecycline and colistin were determined, followed by detection of carbapenemase genes. A bla VIM gene was detected in ~95 % of isolates; bla OXA-23 and bla OXA-40 genes were also prevalent. Diabetic patients were significantly more likely to carry carbapenem-resistant isolates. Carbapenem-resistant A. baumannii is a serious problem in diabetic patients, and molecular detection of resistance mechanisms in these isolates is required.

scholarly journals Dissemination of multiple carbapenem-resistant clones of Acinetobacter baumannii in the Eastern District of Saudi Arabia

2015 ◽  
Vol 6 ◽  
Author(s):  
Abdulrahman A. Al-Sultan ◽  
Benjamin A. Evans ◽  
Elsayed Aboulmagd ◽  
Ahmed A. Al-Qahtani ◽  
Marie Fe F. Bohol ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Acinetobacter Baumannii ◽  
Carbapenem Resistant

scholarly journals Prevalence of High-Risk Antibiotic Resistant Acinetobacter baumannii in the Holy Cities of Makkah and Al-Madinah

2021 ◽  
Vol 15 (1) ◽  
pp. 145-151
Author(s):  
Abdulrahman A. Al-Sultan
Keyword(s):  
Saudi Arabia ◽  
Acinetobacter Baumannii ◽  
Public Health Problem ◽  
Clonal Diversity ◽  
Pulse Field ◽  
Global Public Health ◽  
Antibiotic Resistant ◽  
Pulse Field Gel Electrophoresis ◽  
Carbapenem Resistant ◽  
International Visitors

Background: Acinetobacter baumannii strains resistant to carbapenems are a global public health problem. Objectives: The aim of the present study is to evaluate the prevalence of genetic fingerprints associated with Metallo β-lactamases in A. baumannii in addition to the clonal diversity of A. baumannii in Makkah and Al-Madinah regions of Saudi Arabia, which receive a high number of international visitors. Methods: Multi-antibiotic resistant A. baumannii isolates were investigated. Bacterial isolation was conducted employing a basic bacteriological technique after confirming the ID of isolates. The antimicrobial susceptibility test was carried out using the Vitek 2 compact system. The molecular clonal diversity of the isolates was determined by Pulse Field Gel Electrophoresis (PFGE). Clusters were analyzed with BioNumerics software version 6.5. Dice coefficient was used for calculating the similarities. Results: The results indicated resistance in 82.5% of A. baumannii isolates against the carbapenems. All the isolates were found to be sensitive to colistin, while 5% of isolates were resistant to tigecycline. The screening of carbapenem-resistant A. baumannii isolates showed that the dissemination of imipenem and meropenem resistance was 81 and 84%, respectively, while the majority of the strains were susceptible to tigecycline and colistin. The blaOXA and blaVIM were the most encountered genes in A. baumannii isolates, while ISAba1 was the prominent insertion sequence. The genetic fingerprinting results (PFGE) revealed two types of epidemic clones: monoclonal and polyclonal models of 17 clusters. Conclusion: The current investigation indicates the diversity in genetic fingerprints of carbapenem-resistant A. baumannii in Makkah and Al-Madinah region of Saudi Arabia, and that two types of epidemic clones are present. It has also been demonstrated that such clones create serious infection dissemination to other parts of the world as heavy pilgrimage traffic is received throughout the year in Makkah and Al-Madinah, especially in the Haj season.

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