scholarly journals Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus)

2021 ◽  
Author(s):  
Ibnu Dwi Buwono ◽  
Iskandar Iskandar ◽  
Roffi Grandiosa
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Acid Content ◽  
Feed Efficiency ◽  
Clarias Gariepinus ◽  
Amino Acid Content ◽  
Transgenic Fish ◽  
Feed Conversion ◽  
Commercial Feed ◽  
The Impact

AbstractClarias gariepinus growth hormone (CgGH) transgenesis was previously used to develop a population of second-generation (G2) transgenic mutiara catfish (C. gariepinus). The third generation of these fish (1-month old fingerlings) had 2–3 times improvement in growth compared to non-transgenic fish in the commercial feed test for 6 weeks of rearing. We assessed the impact of CgGH transgene expression on growth and protein and amino acid content of the G3 generation of these transgenic mutiara catfish relative to non-transgenic catfish. Since variation in composition of feed mixes can affect protein and amino acid content of fish, we tested three mixtures of commercial feed and boiled tuna (Euthynnus affinis): feed A (50:50 feed to tuna), B (65:35), and C (80:20) to transgenic catfish. Feed A* (50:50) was fed as a control to non-transgenic catfish. Feed efficiency, including feed conversion ratio and protein use efficiency (i.e., protein retention and protein productive value), was assessed. Feed efficiency, protein content, and essential amino acid content in G3 transgenic catfish (feed A and B) were higher than in non-transgenic fish (feed A*). The latter were deficient in lysine and methionine. Transgenic catfish fed with feed C (80:20) showed lysine deficiency and lower growth than fish fed feeds A and B. Feed B (65:35) was the optimal feed mixture utilized; it increased growth, protein levels, and feed conversion efficiency in G3 catfish. The growth of transgenic fish was higher than non-transgenic fish when supported by feeding with balanced nutrients.

scholarly journals 100 Sequential feeding with diets that differ in amino acid content can significantly increase performance of growing and finishing pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 81-81
Author(s):  
Alini Veira ◽  
Luan S Santos ◽  
Alicia Fraga ◽  
Paulo Campos ◽  
Raphael Caetano ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Phase 1 ◽  
Amino Acid Content ◽  
Experimental Period ◽  
Finishing Pigs ◽  
Phase 2 ◽  
Nutrient Metabolism ◽  
Feeding Programs ◽  
The Impact

Abstract Recent studies have shown that feed intake, nutrient metabolism and utilization may vary during the 24-h circadian period. In this regard, this study aimed at evaluating the impact on performance from the switching of conventional to sequential feeding programs with diets that differ in amino acid content over the day for growing–finishing pigs. Sixty-eight 25-kg (±2.04) BW barrows were assigned to 4 feeding programs (17 animals per treatment): 1) conventional feeding (CONV), in which pigs received 100% of standardized ileal digestible (SID) AA recommendations for the entire day; 2) sequential feeding (SEQ80-120), providing 80% SID AA recommendations from 2400 to 1159 h and 120% from 1200 to 2359 h; 3) sequential feeding (SEQ70-130) providing 70% SID AA recommendations from 2400 to 1159 h and 130% from 1200 to 2359 h; and 4) sequential feeding (SEQ60-140) providing 60% SID AA recommendations from 2400 to 1159 h and 140% from 1200 to 2359 h. The experimental period lasted 82 d and was subdivided in 3 phases: phase 1 (0 to 28 d), phase 2 (29 to 54 d) and phase 3 (55 to 82 d). The data were analyzed using the MIXED procedure in SAS (SAS Inst. Inc., Cary, NC). SEQ80-120 and SEQ60-140 did not improve performance compared to CONV (P > 0.05). However, ADFI, ADG and BW was higher for SEQ70-130 than CONV during phase 1 (1.49 vs 1.3 kg/d; 0.74 vs 0.65 kg/d; 46.55 vs 43.40 kg, respectively; P < 0.05). During phase 2, BW tended to be higher for SEQ70-130 than CONV (69.20 vs 63.60 kg; P = 0.08). In the entire experimental period, ADFI tended to be higher for SEQ70-130 than CONV (2.08 vs 1.89 kg/d; P = 0.10). According to our results, sequential feeding program improves performance of growing–finishing at the beginning of the period.

scholarly journals Nutritional Value of African Catfish (Clarias gariepinus) Meat

2021 ◽  
pp. 31-39
Author(s):  
H. E. Abdel- Mobdy ◽  
H. A. Abdel-Aal ◽  
S. L. Souzan ◽  
A. G. Nassar
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Amino Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Clarias Gariepinus ◽  
Amino Acid Content ◽  
African Catfish ◽  
Oleic Acid Content ◽  
High Oleic

The purpose of this study was to figure out catfish meat's chemical composition, mineral content, amino acid composition, and fatty acid profile. Moisture, protein, lipid, and ash content were measured at 71.30%, 19.03%, 8.10%, and 1.5%, respectively. Catfish meat had higher levels of calcium, phosphorus, and iron, with 304.82, 279.45 and 17.03 mg/100 g, respectively. The essential amino acid content was 41.81 g/100g protein. Oleic, linoleic, and palmitic acids were the most common fatty acids present in catfish meat. Oleic acid made up more than a third of the fatty acid content in catfish meat. Because of its high oleic acid content, catfish meat should be considered because it has been linked to a lower risk of cardiovascular disease.

Study on the Influence of Soil and Fertilization on Amino Acid Content in Oil Linen

2017 ◽  
Vol 68 (4) ◽  
pp. 776-780 ◽  
Author(s):  
Georgeta Pop ◽  
Ersilia Alexa ◽  
Tiberiu Iancu ◽  
Florin Imbrea ◽  
Adrian Laza
Keyword(s):  
Amino Acids ◽  
Veterinary Medicine ◽  
Amino Acid ◽  
Linum Usitatissimum ◽  
Acid Content ◽  
Amino Acid Content ◽  
Research Station ◽  
Linum Usitatissimum L ◽  
Oil Plant ◽  
The Impact

Research results on oil linen (Linum usitatissimum L.) point out the importance of this crop not only as an oil plant but also as a plant with multiple opportunities of diversification. The experiment was set at the Didactic and Research Station within the Banat�s University of Agricultural Sciences and Veterinary Medicine King Michael I of Romania from Timisoara. The goal of this paper is to analyse the impact of soil and fertilization on amino acid content level and structure in oil flax vegetal protein. Cultivar plays a determining role on the content of amino acids in oil flax vegetal protein: the sum of amino acids ranges between 16.21 and 22.44 g/100 g of grits. Fertilization changes the amount of amino acids: the sum of amino acids ranges between 17.08 and 23.32 g/100 g of grits. Fertilization also determines changes of the level and structure of amino acids in oil flax protein.

scholarly journals The Amino Acid Composition of Human Pituitary Growth Hormone

1962 ◽  
Vol 45 (4) ◽  
pp. 176-178 ◽  
Author(s):  
Jonathan S. Dixon ◽  
Choh Hao Li
Keyword(s):  
Molecular Weight ◽  
Growth Hormone ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Acid Content ◽  
Empirical Formula ◽  
Amino Acid Content ◽  
Human Pituitary ◽  
Pituitary Growth Hormone

The amino acid content in human hypophyseal growth hormone has been determined by chromatography on resin columns. On the basis of 29,000 for the molecular weight, the empirical formula of the hormone was obtained: lys13his5arg14asp27 thr14ser23glu34pro12gly13ala12-(1/2 cys)6val12met4-ileu10leu31tyr10phe15try1(NH3)32.

scholarly journals The effects of insulin and adrenaline on the amino-acid content of blood

1933 ◽  
Vol 27 (5) ◽  
pp. 1648-1654 ◽  
Author(s):  
James Murray Luck ◽  
Stanley Wallace Morse
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content

scholarly journals A CONTRIBUTION TO THE STUDY OF THE AMINO-ACID CONTENT OF THE BLOOD

1915 ◽  
Vol 21 (3) ◽  
pp. 511-537
Author(s):  
Paul György ◽  
Edgard Zunz
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

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