Polar Metabolites from the Actinobacterium Isoptericola chiayiensis Isolated from Mangrove Soil

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)

Scientific Reports ◽

10.1038/s41598-021-92179-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jessica Moraes Malheiros ◽

Banny Silva Barbosa Correia ◽

Caroline Ceribeli ◽

Daniel Rodrigues Cardoso ◽

Luiz Alberto Colnago ◽

...

Keyword(s):

Ketone Bodies ◽

Rumen Fluid ◽

Bos Indicus ◽

Metabolite Profile ◽

Polar Compounds ◽

Metabolome Analysis ◽

Ruminal Fluid ◽

Polar Metabolites ◽

Close Relationship ◽

Synthesis And Degradation

AbstractWe conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.

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Phosphate solubilizing bacteria isolated from Tuban mangrove soil, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/762/1/012007 ◽

2021 ◽

Vol 762 (1) ◽

pp. 012007

Author(s):

Fatimah ◽

I N Annizah ◽

D D Alawiyah ◽

R D Susetyo ◽

T Surtiningsih ◽

...

Keyword(s):

Phosphate Solubilizing Bacteria ◽

Mangrove Soil ◽

Phosphate Solubilizing

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Effect of Different Drying Methods on the Nutritional Value of Hibiscus sabdariffa Calyces as Revealed by NMR Metabolomics

Molecules ◽

10.3390/molecules26061675 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1675

Author(s):

Sengnolotha Marak ◽

Elena Shumilina ◽

Nutan Kaushik ◽

Eva Falch ◽

Alexander Dikiy

Keyword(s):

Nutritional Value ◽

2D Nmr ◽

Proximate Analysis ◽

Ash Content ◽

Drying Methods ◽

Hibiscus Sabdariffa ◽

Nmr Metabolomics ◽

Polar Metabolites ◽

2D Nmr Spectra ◽

Plant Processing

Red mature calyces of Hibiscus sabdariffa were collected from 16 different locations in Meghalaya, India. Samples were processed using shade drying (SD) and tray drying (TD). NMR spectroscopy was used to assess the metabolic composition of the calyces. In this study, 18 polar metabolites were assigned using 1D and 2D NMR spectra, and 10 of them were quantified. Proximate analysis showed that the TD method is more efficient at reducing moisture and maintaining the ash content of the Hibiscus biomass. NMR metabolomics indicates that the metabolite composition significantly differs between SD and TD samples and is more stable in TD plant processing. The differences in post-harvest drying has a greater impact on the metabolite composition of Hibiscus than the plant location.

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Evaluation of Antioxidative and Cytotoxic Activities of Streptomyces pluripotens MUSC 137 Isolated from Mangrove Soil in Malaysia

Frontiers in Microbiology ◽

10.3389/fmicb.2015.01398 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 32

Author(s):

Hooi-Leng Ser ◽

Nurul-Syakima Ab Mutalib ◽

Wai-Fong Yin ◽

Kok-Gan Chan ◽

Bey-Hing Goh ◽

...

Keyword(s):

Cytotoxic Activities ◽

Mangrove Soil

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Airway synthesis of 20-hydroxyeicosatetraenoic acid: metabolism by cyclooxygenase to a bronchodilator

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1999.276.2.l280 ◽

1999 ◽

Vol 276 (2) ◽

pp. L280-L288 ◽

Cited By ~ 15

Author(s):

Elizabeth R. Jacobs ◽

Richard M. Effros ◽

John R. Falck ◽

K. Malla Reddy ◽

William B. Campbell ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Arachidonic Acid ◽

Airway Resistance ◽

Acid Metabolism ◽

Gas Chromatography Mass Spectrometry ◽

Dependent Manner ◽

Hydroxyeicosatetraenoic Acid ◽

Polar Metabolites ◽

H 20

Rabbit airway tissue is a particularly rich source of cytochrome P-4504A protein, but very little information regarding the effect(s) of 20-hydroxyeicosatetraenoic acid (20-HETE) on bronchial tone is available. Our studies examined the response of rabbit bronchial rings to 20-HETE and the metabolism of arachidonic acid and 20-HETE from airway microsomes. 20-HETE (10−8 to 10−6 M) produced a concentration-dependent relaxation of bronchial rings precontracted with KCl or histamine but not with carbachol. Relaxation to 20-HETE was blocked by indomethacin or epithelium removal, consistent with the conversion of 20-HETE to a bronchial relaxant by epithelial cyclooxygenase. A cyclooxygenase product of 20-HETE also elicited relaxation of bronchial rings. [14C]arachidonic acid was converted by airway microsomes to products that comigrated with authentic 20-HETE (confirmed by gas chromatography-mass spectrometry as 19- and 20-HETE) and to unidentified polar metabolites. [3H]20-HETE was metabolized to indomethacin-inhibitable products. These data suggest that 20-HETE is an endogenous product of rabbit airway tissue and may modulate airway resistance in a cyclooxygenase-dependent manner.

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