scholarly journals Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jessica Moraes Malheiros ◽  
Banny Silva Barbosa Correia ◽  
Caroline Ceribeli ◽  
Daniel Rodrigues Cardoso ◽  
Luiz Alberto Colnago ◽  
...  
Keyword(s):  
Ketone Bodies ◽  
Rumen Fluid ◽  
Bos Indicus ◽  
Metabolite Profile ◽  
Polar Compounds ◽  
Metabolome Analysis ◽  
Ruminal Fluid ◽  
Polar Metabolites ◽  
Close Relationship ◽  
Synthesis And Degradation

AbstractWe conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.

Alterations in Ruminal Fluid and Serum Biochemical Constituents in Goats Affected with Ruminal Acidosis

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
P. R. Chavelikar ◽  
G. and Neha Rao C. Mandali ◽  
Neha Rao
Keyword(s):  
Rumen Fluid ◽  
Clinical Signs ◽  
Small Ruminants ◽  
Ruminal Fluid ◽  
Mean Values ◽  
Biochemical Constituents ◽  
Ruminal Acidosis ◽  
Methylene Blue Reduction ◽  
Serum Biochemical ◽  
The Mean

Ruminal acidosis is an important clinical emergency in small ruminants. In this study, eight healthy farm goats and 24 goats presented at TVCC of the college of Veterinary Sciences and A.H., Anand with clinical signs of ruminal acidosis and having rumen liquor pH below 6 were examined for alterations in the ruminal fluid and serum biochemical parameters. Among various rumen fluid parameters evaluated, the mean values of rumen fluid pH decreased significantly (4.71±0.11 vs. 6.90±0.10), while sediment activity time (46.67±1.20 vs. 24.50±0.78 min) and methylene blue reduction time (29.50±0.73 vs. 10.03±0.27 min) increased significantly in acidotic goats. The normal greenish, aromatic viscous color, odour and consistency of rumen fluid of healthy goats also changed to milky grey/creamy, sour/pungent watery in acidotic goats. The rumen protozoal activity decreased to nil in acidotic goats as compared to the healthy goats. Among various serum biochemical constituents, the mean values of glucose (92.43±1.37 vs. 74.13±1.83 mg/dl), BUN (26.49±0.47 vs. 22.63±1.19 mg/dl), serum creatinine (01.01±0.02 vs. 00.83±0.02 mg/dl) and albumin (03.22±0.03 vs. 03.05±0.05 g/dl), ALT (56.75±1.55 vs. 27.88±1.14 IU/L) and AST (93.25±1.82 vs. 54.00±1.75 IU/L), increased significantly, while there was significant decrease in serum calcium (09.09±0.14 vs. 10.29±0.08 mg/dl) in acidotic goats. The mean values of alkaline phosphatase (IU/L) in acidotic goats increased non-significantly from the base values of healthy goats.

scholarly journals Diversity of methanogens in ruminants in Queensland

2008 ◽  
Vol 48 (7) ◽  
pp. 722 ◽  
Author(s):  
D. Ouwerkerk ◽  
A. F. Turner ◽  
A. V. Klieve
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Rumen Fluid ◽  
Bos Indicus ◽  
Methane Emissions ◽  
Molecular Techniques ◽  
Feed Conversion ◽  
Methanogen Community ◽  
Gradient Gel Electrophoresis ◽  
Reduce Emissions ◽  
Cattle And Sheep

Methane emissions from ruminant livestock represent a loss of carbon during feed conversion, which has implications for both animal productivity and the environment because this gas is considered to be one of the more potent forms of greenhouses gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, a thorough knowledge of the diversity of these microbes in different breeds of cattle and sheep, as well as in response to different diets, is required. A study was undertaken using the molecular techniques denaturing gradient gel electrophoresis, DNA cloning and DNA sequence analysis to define the extent of diversity among methanogens in ruminants, particularly Bos indicus cross cattle, on differing forages in Queensland. It was found that the diversity of methanogens in forage-fed cattle in Queensland was greater than in grain-fed cattle but there was little variability in methanogen community composition between cattle fed different forages. The species that dominate the rumen microbial communities of B. indicus cross cattle are from the genus Methanobrevibacter, although rumen-fluid inoculated digestors fed Leucaena leucocephala leaf were populated with Methanosphaera-like strains, with the Methanobrevibacter-like strains displaced. If ruminant methane emissions are to be reduced, then antimethanogen bioactives that target both broad groups of ruminant methanogens are most likely to be needed, and as a part of an integrated suite of approaches that redirect rumen fermentation towards other more useful end products.

scholarly journals Comparison of Faecal versus Rumen Inocula for the Estimation of NDF Digestibility

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 928 ◽  
Author(s):  
Maria Chiaravalli ◽  
Luca Rapetti ◽  
Andrea Rota Graziosi ◽  
Gianluca Galassi ◽  
Gianni Matteo Crovetto ◽  
...  
Keyword(s):  
Incubation Time ◽  
Rumen Fluid ◽  
Maize Silage ◽  
Ruminal Fluid ◽  
Feed Ingredients ◽  
Neutral Detergent Fibre ◽  
Lactating Cows ◽  
The Mean

Cow faeces have been investigated as alternative inoculum to replace rumen fluid to determine neutral detergent fibre (NDF) digestibility (NDFD). Aims of this study were to estimate: (1) the NDFD (48 h) of feed ingredients using a rumen inoculum in comparison with faecal inocula from cows fed diets with different forage basis; (2) the undigestible NDF (uNDF) at 240 and 360 h with ruminal fluid and faecal inocula from lactating cows fed two different diets. At 48 h incubation, the NDFD was affected both by feed and type of inoculum (p < 0.01) and by their interaction (p = 0.03). Overall, the mean NDFD was higher for rumen inoculum than for faecal inocula (585 vs. 389 g/kg NDF, p < 0.05), and faecal inoculum obtained from cows fed hay-based diets gave lower NDFD than those from cows fed maize silage (367 vs. 440 g/kg, p < 0.05). At long incubation times, the average uNDF was affected by substrate, inoculum and incubation time (p < 0.01), but not by their interactions. For each inoculum, significantly lower values were obtained at 360 than at 240 h. Regressions between uNDF with rumen and with the tested faecal inocula resulted in r2 ≥ 0.98. Despite the differences at 48 h, the uNDF showed that faecal inoculum could replace rumen fluid at longer incubation times.

Cellular mechanisms of the antiammoniagenic effect of ketone bodies in the dog

1980 ◽  
Vol 239 (5) ◽  
pp. F420-F426 ◽  
Author(s):  
G. Lemieux ◽  
C. Pichette ◽  
P. Vinay ◽  
A. Gougoux
Keyword(s):  
Ketone Bodies ◽  
Glutamate Release ◽  
Metabolite Profile ◽  
Redox Potentials ◽  
Glutamine Concentration ◽  
Cellular Mechanisms ◽  
Total Blood ◽  
Before And After ◽  
Beta Hydroxybutyrate ◽  
Proportional Decrease

To investigate the mechanisms of the antiammoniagenic effect of ketone bodies, acidotic dogs (NH4Cl) were infused with either beta-hydroxybutyrate or acetoacetate. Total blood ketones ranged from 2 to 4 mM. Renal ammoniagenesis fell by a mean of 53%, with a proportional decrease in glutamine extraction. Glutamate release in the renal vein rose, renal extraction of lactate fell, and aspartate and alanine production decreased. Study of the metabolite profile of the renal cortex by the freeze-clamp technique before and after ketone infusion showed that tissue glutamine concentration was unchanged, whereas glutamate, alpha-ketoglutarate, malate, and citrate rose. The intermediates of the gluconeogenic pathway, such as phosphoenolypyruvate, 2-phosphoglycerate, 3-phosphoglycerate, and glucose-6-phosphate, fell significantly. The redox state as calculated from the free NAD+/NADH ratios in the cytosolic (lactate dehydrogenase) and the mitochondrial (glutamate dehydrogenase and beta-hydroxybutyrate dehydrogenase) compartments was reduced. The present study suggests that ketone bodies inhibit renal ammoniagenesis through increased generation of alpha-ketoglutarate (metabolic or bicarbonate effect) and a decrease in the mitochondrial and cytosolic redox potentials in the kidney.

scholarly journals Effects of Freeze-Storing of Rumen Fluid and Sterilization Conditions on Lipopolysaccharide Concentrations of Ruminal Fluid

2012 ◽  
Vol 65 (4) ◽  
pp. 278-280
Author(s):  
Takuya MATSUMOTO ◽  
Tsutai OOHASHI ◽  
Hiroshi SAWADA ◽  
Shozo ARAI
Keyword(s):  
Rumen Fluid ◽  
Ruminal Fluid

The effect of dietary protein and energy sources on ruminal accumulation of low molecular weight peptides in sheep

1995 ◽  
Vol 1995 ◽  
pp. 92-92
Author(s):  
M. Danesh Mesgaran ◽  
D.S. Parker
Keyword(s):  
Molecular Weight ◽  
Dietary Protein ◽  
Rumen Fluid ◽  
Energy Sources ◽  
Low Molecular Weight ◽  
Ruminal Fluid ◽  
Available Energy

Peptides are intermediates in the conversion of ingested protein to ammonia in the rumen and their presence in rumen fluid is influenced by the nature and amount of protein in the diet (2). Transient accumulation of peptides occurs after feeding and then their concentration declines (1,6). However, it is not clear what properties of natually occuring peptide moieties result is increased resistance to degradation in the rumen (5). The objective of this research was to investigate the effect of altering the dietary sources of available energy and protein on the accumulation of peptides in the ruminal fluid.

Rumen fermentation response to a direct-fed xylanase enzyme preparation from Thermomyces lanuginosus in sheep

2006 ◽  
Vol 54 (3) ◽  
pp. 333-342 ◽  
Author(s):  
V. Jurkovich ◽  
J. Kutasi ◽  
Hedvig Fébel ◽  
J. Reiczigel ◽  
E. Brydl ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Enzyme Preparation ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Xylanase Activity ◽  
Rumen Fermentation ◽  
Thermomyces Lanuginosus ◽  
Ruminal Fluid ◽  
Xylanase Enzyme ◽  
Fermentation Parameters

A study was conducted to obtain data on the effects of a fungal fibrolytic enzyme preparation (Rumino-zyme, with 250 FXU/g xylanase activities) from Thermomyces lanuginosus on some rumen fermentation parameters in sheep. Ruminal fluid samples were taken just before the morning feeding and then 2 h and 4 h after feeding. Xylanase activity, pH, concentration of ammonia and volatile fatty acids were measured. The enzyme supplementation did not affect the pH but increased the xylanase activity and the total VFA concentration of the rumen fluid. The molar proportion of acetate increased, propionate was not affected and butyrate decreased after enzyme administration. The concentration of ammonia also decreased after supplementation with the enzyme product. It can be concluded that the xylanase enzyme preparation from T. lanuginosus induced favourable changes in the major rumen fermentation parameters in sheep.

In Vitro Conversion of ß-Carotene to Retinal in Bovine Rumen Fluid by a Recombinant ß-Carotene- 15, 15’-Monooxygenase

2012 ◽  
Vol 82 (2) ◽  
pp. 94-103 ◽  
Author(s):  
García-López ◽  
González-Gallardo ◽  
Antaramián ◽  
L. González-Dávalos ◽  
Shimada ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Meat Products ◽  
Beta Carotene ◽  
Future Research ◽  
In Vitro Digestibility ◽  
Optimum Conditions ◽  
Recombinant Bacteria ◽  
Ruminal Fluid ◽  
E Coli

Pasture-fed cattle yield carcasses with yellow fat; consumers often reject the resulting meat products because they assume they come from old and/or culled animals. Recombinant bacteria expressing beta-carotene 15, 15’-monooxygenase, introduced into the rumen of the animal, might help to reduce the coloration since this enzyme converts carotene to retinal, thereby eliminating the source of yellowness. The goal of this work was to evaluate the effect of a recombinant beta-carotene 15, 15’-monooxygenase (BCMO1) from Gallus gallus, expressed in Escherichia coli. The genetically modified microbe was introduced into ruminal fluid, and carotene conversion to retinal was measured. Under optimum conditions the enzyme produced 6.8 nmol of retinal per 1 mg of protein in 1 hour at 37 °C. The data on in vitro digestibility in ruminal fluid showed no differences in beta-carotene breakdown or in retinal production (p > 0.1) between E. coli with pBAD vector alone and E. coli with pBAD/BCMO1. The pBAD/BCMO1 plasmid was stable in E. coli for 750 generations. These results indicate that the protein did not break beta-carotene into retinal in ruminal fluid, perhaps due to its location in the periplasmic space in E. coli. Future research must consider strategies to release the enzyme into the rumen environment.

scholarly journals Ruminal parameters and nitrogen balance in sheep fed diets containing residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (5) ◽  
pp. 3411 ◽  
Author(s):  
Luiz Juliano Valério Geron ◽  
Jocilaine Garcia ◽  
Fabiana Gomes Costa ◽  
Sílvia Cristina de Aguiar ◽  
Edimar Barbosa Oliveira ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Experimental Period ◽  
Ammonia Nitrogen ◽  
Linear Increase ◽  
Ruminal Fluid ◽  
Linear Effect ◽  
Mixed Breed ◽  
Ph Level ◽  
Male Sheep

<p>This study measured the ruminal parameters, feed intake, fecal and urinary N production, and N balance (NB) in sheep fed increasing levels of residue from the extraction of tamarind pulp (RETP) on a 0.0%, 5.0%, 10.0%, and 15.0% dry matter (DM) basis. Four mixed-breed male sheep weighing 40.38 ± 2.10 kg of body weight were distributed in a 4 × 4 Latin square design. The animals were allocated to metabolism cages and fed twice a day. Each experimental period lasted 20 days. The pH and concentration of ammonia nitrogen (NH3-N) in ruminal fluid were measured. Nitrogen intake (NI), fecal N (FN), urinary N (UN), absorbed N (AN), and NB were expressed in g·day-1, percentage of consumed nitrogen (CN), and g·kg-1 of metabolic weight (g·(kg0.75)-1. Statistical analysis of these variables was performed by evaluating the variance and regression at 5% probability. The levels of RETP did not alter (p&gt;0.05) the pH level or NH3-N concentration in the rumen fluid, but a quadratic behavior for the same values after feeding (p&lt;0.05) was observed. The inclusion of RETP in the diet did not change (p&gt;0.05) NI, UN, the AN in g·day-1 and g·(kg0.75)-1, NB in g·day-1 and g·(kg0.75)-1, or the percentage of CN. However, the AN as a percentage of CN showed a linear effect (p&lt;0.05) with the inclusion of RETP in experimental diets. For FN in g·day-1 and g·(kg0.75) -1, no difference was observed (p&gt;0.05) with the inclusion of RETP, but for FN expressed as a percentage of CN, a linear increase (p&lt;0.05) was observed with the inclusion of RETP. Thus, we concluded that diets with up to 15.0% RETP do not alter the pH and NH3-N in ruminal fluid, NI, UN, or NB. In addition, the inclusion of 15.0% RETP has a laxative affect. </p>

scholarly journals Influence of food, storage temperature, and time on the extracorporeal viability of ruminal fluid of cattle

2018 ◽  
Vol 39 (3) ◽  
pp. 1181
Author(s):  
Camila Cecilia Martin ◽  
Ayrton Rodrigo Hilgert ◽  
Erica Cristina Bueno do Prado Guirro
Keyword(s):  
Ambient Temperature ◽  
Storage Temperature ◽  
Rumen Fluid ◽  
Chloride Content ◽  
Water Bath ◽  
Food Storage ◽  
Ruminal Fluid ◽  
Influence Of Food ◽  
Group A

Rumen fluid obtained from slaughter houses is sometimes used for transfaunation. In this study, we evaluated the ruminal fluid of cattle fed on pasture or pasture and concentrate and slaughtered recently. The rumen fluid samples were stored in a water bath, at ambient temperature, cooled, or frozen and subjected to physical, chemical, and microbiological evaluation for 24 h. The color, consistency, and odor changed primarily in the cooled samples, followed by the ambient temperature and water bath samples. The pH of fresh ruminal fluid was 7.5 in the pasture group and 6.4 in the pasture and concentrate group. The methylene blue reduction time of the fresh ruminal fluid was 2.35 min in the pasture group and 1.86 min in the pasture and concentrate group; the best values were observed in the water bath group. The chloride content was 15.7 mEq/l in the pasture group and 16.3±3.6 mEq/l in the pasture and concentrate group. A predominance of gram-negative bacteria was observed. The concentration of protozoa was 51.5 in the pasture group and 47.5 × 104/ml in the pasture and concentrate group, with a slight predominance of small protozoa; motility was better in the water bath samples than in the ambient temperature, cooled, and frozen samples. The viability of ruminal fluid collected from freshly slaughtered cattle was influenced by food provided in vivo; however, changes as a function of time and storage temperature were more remarkable. The rumen fluid was viable for up to 9 h when stored in the water bath (38°C) and 2 h at ambient temperature, and it was observed to be nonviable when subjected to cooling or freezing.

Sign in / Sign up

Export Citation Format

Share Document