Biochemical and growth performance of the aquatic macrophyte Azolla filiculoides to sub-chronic exposure to cylindrospermopsin

This study aimed to investigate the effects of chronic exposure to low levels of dietary aflatoxin B1 (AFB1) on growth performance, apparent total tract digestibility and intestinal health in pigs. In a 102-day experiment, fourteen barrows (Duroc×Landrace×Yorkshire, initial BW = 38.21 ± 0.45 kg) were randomly divided into control (CON, basal diet) and AFB1 groups (the basal diet supplemented with 280 μg/kg AFB1). Results revealed that the AFB1 exposure decreased the final BW, ADFI and ADG in pigs (p < 0.10). AFB1 exposure also decreased the apparent total tract digestibility of dry mater and gross energy at 50 to 75 kg and 105 to 135 kg stages, and decreased the apparent total tract digestibility of ether extract at 75 to 105 kg stage (p < 0.05). Meanwhile, AFB1 exposure increased serum diamine oxidase activity and reduced the mRNA abundance of sodium-glucose cotransporter 1, solute carrier family 7 member 1 and zonula occluden-1 in the jejunal mucosa (p < 0.05). Furthermore, AFB1 exposure decreased superoxide dismutase activity (p < 0.05) and increased 8-hydroxy-2′-deoxyguanosine content (p < 0.10) in jejunal mucosa. AFB1 exposure also increased tumor necrosis factor-α, interleukin-1β and transforming growth factor-β mRNA abundance in jejunal mucosa and upregulated Escherichia coli population in colon (p < 0.05). The data indicated that chronic exposure to low levels of dietary AFB1 suppressed growth performance, reduced the apparent total tract digestibility and damaged intestinal barrier integrity in pigs, which could be associated with the decreased intestinal antioxidant capacity and the increased pro-inflammatory cytokine production.

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Effect of lead nitrate on survival rate and chronic exposure on growth performance of grass carp (Ctenopharyngodon idella)

Journal of Toxicology and Environmental Health Sciences ◽

10.5897/jtehs12.049 ◽

2012 ◽

Vol 4 (10) ◽

pp. 171-174

Author(s):

Esmail Gharedaashi

Keyword(s):

Survival Rate ◽

Growth Performance ◽

Grass Carp ◽

Chronic Exposure ◽

Lead Nitrate ◽

Ctenopharyngodon Idella ◽

Grass Carp Ctenopharyngodon Idella

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Development of apical pits in chloride cells of the gills of Pimephales promelas after chronic exposure to acid water

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076044 ◽

1983 ◽

Vol 41 ◽

pp. 462-463

Author(s):

Richard L. Leino ◽

Jon G. Anderson ◽

J. Howard McCormick

Keyword(s):

Confidence Interval ◽

Chronic Exposure ◽

Lake Superior ◽

Pimephales Promelas ◽

Chloride Cells ◽

Fathead Minnows ◽

Secondary Lamellae ◽

Untreated Water ◽

Water Ph ◽

Flow Through

Groups of 12 fathead minnows were exposed for 129 days to Lake Superior water acidified (pH 5.0, 5.5, 6.0 or 6.5) with reagent grade H2SO4 by means of a multichannel toxicant system for flow-through bioassays. Untreated water (pH 7.5) had the following properties: hardness 45.3 ± 0.3 (95% confidence interval) mg/1 as CaCO3; alkalinity 42.6 ± 0.2 mg/1; Cl- 0.03 meq/1; Na+ 0.05 meq/1; K+ 0.01 meq/1; Ca2+ 0.68 meq/1; Mg2+ 0.26 meq/1; dissolved O2 5.8 ± 0.3 mg/1; free CO2 3.2 ± 0.4 mg/1; T= 24.3 ± 0.1°C. The 1st, 2nd and 3rd gills were subsequently processed for LM (methacrylate), TEM and SEM respectively.Three changes involving chloride cells were correlated with increasing acidity: 1) the appearance of apical pits (figs. 2,5 as compared to figs. 1, 3,4) in chloride cells (about 22% of the chloride cells had pits at pH 5.0); 2) increases in their numbers and 3) increases in the % of these cells in the epithelium of the secondary lamellae.

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The Effect of Vitamin A on the Intermittent Nitrogen Dioxide Gas Exposure in Hamsters

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090865 ◽

1976 ◽

Vol 34 ◽

pp. 176-177

Author(s):

J.C.S. Kim ◽

M.G. Jourden ◽

E.S. Carlisle

Keyword(s):

Electron Microscopy ◽

Vitamin A ◽

Nitrogen Dioxide ◽

Chronic Exposure ◽

Light And Electron Microscopy ◽

Specific Effect ◽

Deficient Diet ◽

Intermittent Exposure ◽

Hypovitaminosis A ◽

Gas Exposure

Chronic exposure to nitrogen dioxide in rodents has shown that injury reaches a maximum after 24 hours, and a reparative adaptive phase follows (1). Damage occurring in the terminal bronchioles and proximal portions of the alveolar ducts in rats has been extensively studied by both light and electron microscopy (1).The present study was undertaken to compare the response of lung tissue to intermittent exposure to 10 ppm of nitrogen dioxide gas for 4 hours per week, while the hamsters were on a vitamin A deficient diet. Ultrastructural observations made from lung tissues obtained from non-gas exposed, hypovitaminosis A animals and gas exposed animals fed a regular commercially prepared diet have been compared to elucidate the specific effect of vitamin A on nitrogen dioxide gas exposure. The interaction occurring between vitamin A and nitrogen dioxide gas has not previously been investigated.

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