Effect of temperature on Colletotrichum truncatum growth, and evaluation of its inoculum potential in soybean seed germination

Soybean production in the system of organic agriculture is not very demanding, and this has been well documented both through experimental results and commercial production. However, one of the biggest problems in organic production is the lack of adequate pre-sowing treatments. Therefore, the aim of this study was to examine the effect of the electrostatic field. This is a physical treatment that was first used for seed treatment in the 18th century but has mostly been neglected since then. Seeds of five soybean genotypes with differently colored seed coats (yellow, green, dark green, brown, and black) were included in this study. The seeds were exposed to different values of direct current (DC) with the following voltages: 0 V (control), 3 V, 6 V, and 9 V, to which the seeds were exposed for 0 min (control), 1 min, and 3 min. After exposing the seeds to the electric field, the physiological properties of seeds and seedlings at the first stage of growth were evaluated. The results show that the effect of the electrostatic field on seed quality depends on the genotype, voltage, and exposure time. The application of DC can be a suitable method for improving seed germination and the initial growth of soybean seedlings. In addition, the results indicate that it is necessary to adjust the DC treatment (voltage and duration of exposure of seeds) to particular genotypes since inadequate treatments may reduce the quality of seeds.

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The expansin EXP1 gene in the elongation zone is induced during soybean embryonic axis germination and differentially expressed in response to ABA and PEG treatments

Seed Science Research ◽

10.1017/s0960258520000379 ◽

2020 ◽

pp. 1-9

Author(s):

Nidia H. Montechiarini ◽

Luciana Delgado ◽

Eligio N. Morandi ◽

Néstor J. Carrillo ◽

Carlos O. Gosparini

Keyword(s):

Cell Wall ◽

Seed Germination ◽

Water Availability ◽

Expression Profiles ◽

Soybean Seed ◽

Embryonic Axis ◽

Growth Potential ◽

Elongation Zone ◽

Degenerate Primers ◽

Embryonic Axes

Abstract During soybean seed germination, the expansive growth potential of the embryonic axes is driven by water uptake while cell wall loosening occurs in cells from the elongation zone (EZ). Expansins are regarded as primary promoters of cell wall remodelling in all plant expansion processes, with the expression profiles of the soybean expansins supporting their cell or tissue specificity. Therefore, we used embryonic axes isolated from whole seed and focused on the EZ to study seed germination. Using a suite of degenerate primers, we amplified an abundantly expressed expansin gene at the EZ during soybean embryonic axis germination, which was identified as EXP1 by in silico analyses. Expression studies showed that EXP1 was induced under germination conditions in distilled water and down-regulated by abscisic acid (ABA), which inhibits soybean germination by physiologically restraining expansion. Moreover, we also identified a time window of ABA responsiveness within the first 6 h of incubation in water, after which ABA lost control of both EXP1 expression and embryonic axis germination, thus confirming the early role of EXP1 in the EZ during this process. By contrast, EXP1 levels in the EZ increased even when germination was impaired by osmotically limiting the water availability required to develop the embryonic axes’ growth potential. We propose that these higher EXP1 levels are involved in the fast germination of soybean embryonic axes as soon as water availability is re-established. Taken together, our results show strong EXP1 expression in the EZ and postulate EXP1 as a target candidate for soybean seed germination control.

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Effect of temperature, salinity, light and time of dehiscence on seed germination and seedling morphology of Calotropis procera from urban habitats

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2014.14305 ◽

2015 ◽

Vol 14 (15) ◽

pp. 1275-1282 ◽

Cited By ~ 1

Author(s):

M Galal Tarek ◽

A Farahat Emad ◽

M El Midany Maha ◽

M Hassan Loutfy

Keyword(s):

Seed Germination ◽

Effect Of Temperature ◽

Calotropis Procera ◽

Urban Habitats ◽

Seedling Morphology

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Effect of Temperature on the Short Chain Fatty Acid-induced Inhibition of Lettuce Seed Germination

PLANT PHYSIOLOGY ◽

10.1104/pp.63.1.61 ◽

1979 ◽

Vol 63 (1) ◽

pp. 61-62 ◽

Cited By ~ 19

Author(s):

Robert R. C. Stewart ◽

Alex M. M. Berrie

Keyword(s):

Fatty Acid ◽

Seed Germination ◽

Short Chain Fatty Acid ◽

Chain Fatty Acid ◽

Short Chain ◽

Effect Of Temperature ◽

Lettuce Seed ◽

Lettuce Seed Germination

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Near-infrared spectroscopy used to predict soybean seed germination and vigour

Seed Science Research ◽

10.1017/s0960258518000119 ◽

2018 ◽

Vol 28 (3) ◽

pp. 245-252 ◽

Cited By ~ 7

Author(s):

Maythem Al-Amery ◽

Robert L. Geneve ◽

Mauricio F. Sanches ◽

Paul R. Armstrong ◽

Elizabeth B. Maghirang ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Seed Germination ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Prediction Models ◽

Soybean Seed ◽

Seed Vigour ◽

Near Infrared Spectra ◽

High Germination ◽

Qualitative Models

AbstractRapid, non-destructive methods for measuring seed germination and vigour are valuable. Standard germination and seed vigour were determined using 81 soybean seed lots. From these data, seed lots were separated into high and low germinating seed lots as well as high, medium and low vigour seed lots. Near-infrared spectra (950–1650 nm) were collected for training and validation samples for each seed category and used to create partial least squares (PLS) prediction models. For both germination and vigour, qualitative models provided better discrimination of high and low performing seed lots compared with quantitative models. The qualitative germination prediction models correctly identified low and high germination seed lots with an accuracy between 85.7 and 89.7%. For seed vigour, qualitative predictions for the 3-category (low, medium and high vigour) models could not adequately separate high and medium vigour seeds. However, the 2-category (low, medium plus high vigour) prediction models could correctly identify low vigour seed lots between 80 and 100% and the medium plus high vigour seed lots between 96.3 and 96.6%. To our knowledge, the current study is the first to provide near-infrared spectroscopy (NIRS)-based predictive models using agronomically meaningful cut-offs for standard germination and vigour on a commercial scale using over 80 seed lots.

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Seed germination ecology of Sumatran fleabane (Conyza sumatrensis) in relations to various environmental parameters

Weed Science ◽

10.1017/wsc.2021.56 ◽

2021 ◽

pp. 1-26

Author(s):

Gulshan Mahajan ◽

Asheneel Prasad ◽

Bhagirath Singh Chauhan

Keyword(s):

Seed Germination ◽

Light Intensity ◽

Chloride Concentration ◽

Soil Surface ◽

Control Treatment ◽

Effect Of Temperature ◽

Burial Depth ◽

Germination Ecology ◽

Wide Range ◽

Dark Conditions

Abstract Sumatran fleabane [Conyza sumatrensis (Retz.) Walker] is an emerging weed in the Australian cropping region. Populations resistant to glyphosate have evolved in Australia, creating the demand for information regarding the seed germination ecology of glyphosate-resistant (R) and glyphosate susceptible (S) populations of C. sumatrensis. A study was conducted to examine the effect of temperature, light intensity, salt stress, osmotic stress, and burial depth on the germination and emergence of two populations (R and S) of C. sumatrensis. Both populations were able to germinate over a wide range of alternating day/night temperatures (15/5 to 35/25 C). In light/dark conditions, the R population had higher germination than the S population at 20/10 and 35/25 C. In the dark, the R population had higher germination than the S population at 25/15 C. In the dark, germination was inhibited at 30/20 C and above. Averaged over populations, seed germination of C. sumatrensis was reduced by 97% at zero light intensity (completely dark conditions) compared with full light intensity. Seed germination of C. sumatrensis reduced by 17 and 85% at an osmotic potential of −0.4, and −0.8 MPa, respectively, compared with the control treatment. The R population had lower germination (57%) than the S population (72%) at a sodium chloride concentration of 80 mM. Seed germination was highest on the soil surface and emergence was reduced by 87 and 90% at burial depths of 0.5 and 1.0 cm, respectively. Knowledge gained from this study suggests that a shallow-tillage operation to bury weed seeds in conventional tillage systems, and retention of high residue cover in a zero-till system on the soil surface may inhibit the germination of C. sumatrensis. This study also warrants that the R population may have a greater risk of invasion over a greater part of a year due to germination over a broader temperature range.

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